水曲柳药材的质量标准研究
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摘要
水曲柳为木樨科梣属植物,其树皮具有抗炎镇痛的效应。常被作为秦皮的替代品使用,主要含香豆素类成分,其中作为秦皮药材鉴定的主要成分秦皮甲素和秦皮乙素的差异很大,并且有些产地的水曲柳中还根本检测不到,目前还没有相应的标准来衡量水曲柳的质量。本文采用HPLC法对水曲柳皮进行了含量测定,从整体上对水曲柳药材的质量进行控制,为水曲柳的开发提供质量控制标准。
实验中采用HPLC法对水曲柳药材中Mandshurin的含量进行研究,实验结果显示样品的稳定性、重现性较好而且3批样品的相似度较高。说明该法测定结果稳定,重现性好,且方法简便、快速、可靠,实际可行,为水曲柳的质量控制提供了新的手段。所以该方法可用于水曲柳药材的鉴定及质量评价。
Fraxinus mandshurica Rupr was Oleaceae Fraxinus plant, it's bark has the effect of anti-inflammation and analgesia,and it is usually used as the succedaneum of the ash brak, its main component is coumarin.The main appraisement index of ash brak drugs is esculin and esculetin,and their difference was very large, at the same time,it was difficult to detect them in the Fraxinus mandshurica Rupr of some habitat, at the present time there are not corresponding standard to judge the quality of the Fraxinus mandshurica Rupr.In this thesis,the content of the Fraxinus mandshurica Rupr were identified by HPLC method, and the quality of the Fraxinus mandshurica Rupr was entirely controlled,so the quality control specification was supplied for the development of the Fraxinus mandshurica Rupr.
In this paper, The Mandshurin content of the Fraxinus mandshurica Rupr was studied by HPLC method. The results were shown that the similarity of the three samples was high,so the measure results of HPLC method was steady,and the repeatability was good,at the same time,the instruments was common,the method was simple、fast、credibility、practice and feasible, so the new facility was supplied for the quality controlling of the Fraxinus mandshurica Rupr,and the HPLC method could be used as the quality evaluation and identification of the Fraxinus mandshurica Rupr.
实验中采用HPLC法对水曲柳药材中Mandshurin的含量进行研究,实验结果显示样品的稳定性、重现性较好而且3批样品的相似度较高。说明该法测定结果稳定,重现性好,且方法简便、快速、可靠,实际可行,为水曲柳的质量控制提供了新的手段。所以该方法可用于水曲柳药材的鉴定及质量评价。
Fraxinus mandshurica Rupr was Oleaceae Fraxinus plant, it's bark has the effect of anti-inflammation and analgesia,and it is usually used as the succedaneum of the ash brak, its main component is coumarin.The main appraisement index of ash brak drugs is esculin and esculetin,and their difference was very large, at the same time,it was difficult to detect them in the Fraxinus mandshurica Rupr of some habitat, at the present time there are not corresponding standard to judge the quality of the Fraxinus mandshurica Rupr.In this thesis,the content of the Fraxinus mandshurica Rupr were identified by HPLC method, and the quality of the Fraxinus mandshurica Rupr was entirely controlled,so the quality control specification was supplied for the development of the Fraxinus mandshurica Rupr.
In this paper, The Mandshurin content of the Fraxinus mandshurica Rupr was studied by HPLC method. The results were shown that the similarity of the three samples was high,so the measure results of HPLC method was steady,and the repeatability was good,at the same time,the instruments was common,the method was simple、fast、credibility、practice and feasible, so the new facility was supplied for the quality controlling of the Fraxinus mandshurica Rupr,and the HPLC method could be used as the quality evaluation and identification of the Fraxinus mandshurica Rupr.
引文
[1]孙三省等.中国梣属植物的研究.植物研究.1985(1).
[2]中华人民共和国药典委员会.中华人民共和国药典(2000版)一部.化学工业出版社,2000.
[3]刘慎谔等,东北木本植物图志[M]科学出版社.1955.
[4]荆涛,张林玉.水曲柳的生长规律[J]内蒙古农业大学学报,2004,25(1):36-39.
[5]崔晓阳,宋金凤,屈明华.土壤水势对水曲柳幼苗水分生态的影响[J]应用生态学报,2004,(12):2237-2244.
[6]王红梅,杨金英,张学政,丰宝林.Ca2+对低温胁迫下水曲柳叶片生理指标的影响[J]东北林业大学学报,2004,32(4):14-15.
[7]吴楚,王政权,范志强等.不同氮浓度和形态比例对水曲柳幼苗叶绿素合成、光合作用以及生物量分配的影响[J].植物生态学报,2003,27(6):771-779.
[8]沈洁梅.水曲柳板材干燥处理的研究[J].工艺与设备,2003(2):19-22.
[9]孙艳玲,鹿振友,何龙.水曲柳木材裂纹尖端应力强度因子研究(英文)[J].Forestry Studies in China,2000,2(2):76-79.
[10]李丰,冯运明,许明怡等.白蜡嫁接水曲柳优树采穗圃建立方法[J].宁夏农林科技,2002,(2):37-38.
[11]Sansei,et al.,Stadies on the Chinese Crude Drug "Forsythiae Fructus",viThe Structure and Antibacterial Activity of Suspersaside isolated from ForsythiaSuspensa,Chem.Pharm.Bull.,1982,32(12),4548-4553.
[12]J.M.Dinwood ie.Initializes smelt of the influence of moisture content and level of stressing on rate of creep and time to failure.W.S.T.1981.15.P125-144.
[13]Nicotra,A.B.,R.L.Chazdon,andS.V.B.Iriarte.1999.Spatialheterogeneityoflightandwoodyseedlingregenerationintropicalw etforests J.Ecology80:6,1908-1926.
[14]Kostova I,Dinchev D,Mikhova B,Iossifova T.Epoxyconiferyl alcohol form Fraxinus oxycarp bark[J],Phytochemistry 2000,53:827.
[15]Dou YH,Bao N,Chen HY,et al.A dynamically modified microflu-idic poly(dimethylsiloxane) chip with electrochemical detection for biological analysis.Electrophoresis,2002,2320,23(20):3558.
[16]Rugger M,Dewey E,Hobbie L,Brown D,Bernasconi P,Turner J,Muday G,Estelle M.Reduced NPA_binding in the tir3 mutant of Arabidopsis is associated with a reduction in polar auxin transport and diverse morphological defects.Plant Cell,1997,9,9:745-757.
[17]Ellsworth D S.CO2 enrichment in a maturing pine forest:are CO2 exchange and water status in the canopy affected.Plant,Cell and Environ,1999,22,22:461-472.
[18]方圣鼎,陈仁通,韦璧瑜.中药现代化的思考[J].中草药,2007,(05).
[19]Sylvia H.Dancan et al.Inhibitory activity of gut bacteria against Escherichia coli O157 mediated by dietary plant metaolites[J].FEMS Microbiology letters,1998,164:283-288.
[20]Effect of angroside in Scrophularia ningpoensis on synthesis of platelet cAMP and plasma PGI_2/TXA_2 in rabbits.Academic Journal of Second Military Medical University,2004,(08):2.
[21]原忠.中药制剂质量评价的研究现状[J]国外医学.中医中药分册,1997,(03).
[22]Paci A,Caire-Maurisier AM,Rieutord A et al.Dual-mode gradient HPLC procedure for the simultaneous determination of chloroquine and proguanil.Journal of Pharmaceutical and Biomedical Analysis,2002,271,27(1):1-7.
[23]Ivanovska N,Iossifova T,Vassileva E,Kostova I.Methods Find Exp Clin Pharmacol 1994,16:557.
[24]Sunil KT,Swapan KM,Anup B,et at.Methoxylated flavones of Fortunella Japonica.Phytochemistry,1975,141,14(1):309.
[25]Chia-Yihchun et al,European Journal of Pharmacology,2001,416;26-32.
[26]Noguchi et al.,Effects of piroxicam and wsculetin on the MDA-MB-231 huaman,Prostaglandins,Leukotrienes Essent [J],Fatty Acids,1995,53:325-329.
[27]China Pharmacopoeia Committee.Pharmacopoeia of the People's Republic of China,2005 volume Ⅰ.Beijing:Chemical Industry Press,2005,:279.
[28]Xiaomin Wang at al.Determination of Ginsenosides in Plant Extracts from Panax ginseng and Panax quinquefolius L.By LC/MS/MS.Anal.Chem,1999,718,71(8):1579-1584.
[29]王宝琴.中药标准化回顾.中成药[J],2000,2(1):22-26.
[30]郑志华.加强中药质量标准研究是实现中药现代化的必要前提.中国药业[J].2001,10(2):4-6.
[31]李毓群.提高中药制剂质量的若干建议.中国中药杂志[J].2002.27(6):471-474.
[32]谢培山.中医药传统文化与现代质量控制[J]中国中西医结合杂志,1998,(11).
[33]Sticher O.Quality Of Ginkgo preparations.Planba Medica[J].1993,(59):2-11.
[34]谢培山.中药制剂色谱指纹图谱(图像)鉴别.中成药[J].2000,(6):391-395.
[35]邹汉法,汪海林.生物色谱技术分离、鉴定和筛选中药活性成分[J]世界科学技术,2000,(02).
[36]高夏红.中草药XRD指纹图谱方法学研究及中药XRD指纹图谱数据库建立[D]四川大学,2005
[37]王晃,李梦龙,覃洁萍,刘仲义.建立中药指纹图谱 保证中药产品质量[J]成都中医药大学学报,2002,(03).
[38]Wei W,Luo GA,Hua GY,et al.Capillary electrochromatographic separation of basic compounds with bare silica as stationary phase.J Chromatogr A,1998,8171-2,817(1-2):65.
[39]姚新生,胡柯.中药复方的现代化研究[J]化学进展,1999,(02).
[40]Romblum RN.Pituitary lession in emiocerebeal injuries[J].Aech.Path.1969,88,88:242.
Nei M.Estimation of Average Heterozygosity and Genetic Distancee from a Small Number of Individuals.Genetics,1978,89,89:583.
[41]Talapatra S K et al.On the chemistry of Indian Orchidaceae plants-Ⅱ.Dengibsin and dengibsinin,the first natural fluorenone derivatives from Dendrobium gibsonii Lindl.Tetrahedron,1985,4113,41(13):2765.
[42]Behr D et al.Studies on Orchidaceae glycosides part 6.Three steroid glycosides of the stigmastare type from Dendrobium ochreatum.Phytochemistry,1976,159,15(9):1403.
[43]葛恩慈.当前中药质量存在的问题及对策[J]浙江中医学院学报,1997,(03).
[44]苏薇薇,刘晓勇,吴忠.传统中药与中药现代化[J]中药材,2000,(08).
[45]M.Perrut,Advances in super critical fluid chromatography,J.Chromatogr.A,1994,658,293-313.
[46]董嘉德,超声技术在中药提取中的应用,中国药业,2002,11(11),55.
[47]杨景亮主编,《中医药工程研究与应用》,北京,中国中医药出版社.
[48]孙严彤.长白山花曲柳和水曲柳化学成份的研究:硕士学位论文.吉林,吉林大学,2004.
[2]中华人民共和国药典委员会.中华人民共和国药典(2000版)一部.化学工业出版社,2000.
[3]刘慎谔等,东北木本植物图志[M]科学出版社.1955.
[4]荆涛,张林玉.水曲柳的生长规律[J]内蒙古农业大学学报,2004,25(1):36-39.
[5]崔晓阳,宋金凤,屈明华.土壤水势对水曲柳幼苗水分生态的影响[J]应用生态学报,2004,(12):2237-2244.
[6]王红梅,杨金英,张学政,丰宝林.Ca2+对低温胁迫下水曲柳叶片生理指标的影响[J]东北林业大学学报,2004,32(4):14-15.
[7]吴楚,王政权,范志强等.不同氮浓度和形态比例对水曲柳幼苗叶绿素合成、光合作用以及生物量分配的影响[J].植物生态学报,2003,27(6):771-779.
[8]沈洁梅.水曲柳板材干燥处理的研究[J].工艺与设备,2003(2):19-22.
[9]孙艳玲,鹿振友,何龙.水曲柳木材裂纹尖端应力强度因子研究(英文)[J].Forestry Studies in China,2000,2(2):76-79.
[10]李丰,冯运明,许明怡等.白蜡嫁接水曲柳优树采穗圃建立方法[J].宁夏农林科技,2002,(2):37-38.
[11]Sansei,et al.,Stadies on the Chinese Crude Drug "Forsythiae Fructus",viThe Structure and Antibacterial Activity of Suspersaside isolated from ForsythiaSuspensa,Chem.Pharm.Bull.,1982,32(12),4548-4553.
[12]J.M.Dinwood ie.Initializes smelt of the influence of moisture content and level of stressing on rate of creep and time to failure.W.S.T.1981.15.P125-144.
[13]Nicotra,A.B.,R.L.Chazdon,andS.V.B.Iriarte.1999.Spatialheterogeneityoflightandwoodyseedlingregenerationintropicalw etforests J.Ecology80:6,1908-1926.
[14]Kostova I,Dinchev D,Mikhova B,Iossifova T.Epoxyconiferyl alcohol form Fraxinus oxycarp bark[J],Phytochemistry 2000,53:827.
[15]Dou YH,Bao N,Chen HY,et al.A dynamically modified microflu-idic poly(dimethylsiloxane) chip with electrochemical detection for biological analysis.Electrophoresis,2002,2320,23(20):3558.
[16]Rugger M,Dewey E,Hobbie L,Brown D,Bernasconi P,Turner J,Muday G,Estelle M.Reduced NPA_binding in the tir3 mutant of Arabidopsis is associated with a reduction in polar auxin transport and diverse morphological defects.Plant Cell,1997,9,9:745-757.
[17]Ellsworth D S.CO2 enrichment in a maturing pine forest:are CO2 exchange and water status in the canopy affected.Plant,Cell and Environ,1999,22,22:461-472.
[18]方圣鼎,陈仁通,韦璧瑜.中药现代化的思考[J].中草药,2007,(05).
[19]Sylvia H.Dancan et al.Inhibitory activity of gut bacteria against Escherichia coli O157 mediated by dietary plant metaolites[J].FEMS Microbiology letters,1998,164:283-288.
[20]Effect of angroside in Scrophularia ningpoensis on synthesis of platelet cAMP and plasma PGI_2/TXA_2 in rabbits.Academic Journal of Second Military Medical University,2004,(08):2.
[21]原忠.中药制剂质量评价的研究现状[J]国外医学.中医中药分册,1997,(03).
[22]Paci A,Caire-Maurisier AM,Rieutord A et al.Dual-mode gradient HPLC procedure for the simultaneous determination of chloroquine and proguanil.Journal of Pharmaceutical and Biomedical Analysis,2002,271,27(1):1-7.
[23]Ivanovska N,Iossifova T,Vassileva E,Kostova I.Methods Find Exp Clin Pharmacol 1994,16:557.
[24]Sunil KT,Swapan KM,Anup B,et at.Methoxylated flavones of Fortunella Japonica.Phytochemistry,1975,141,14(1):309.
[25]Chia-Yihchun et al,European Journal of Pharmacology,2001,416;26-32.
[26]Noguchi et al.,Effects of piroxicam and wsculetin on the MDA-MB-231 huaman,Prostaglandins,Leukotrienes Essent [J],Fatty Acids,1995,53:325-329.
[27]China Pharmacopoeia Committee.Pharmacopoeia of the People's Republic of China,2005 volume Ⅰ.Beijing:Chemical Industry Press,2005,:279.
[28]Xiaomin Wang at al.Determination of Ginsenosides in Plant Extracts from Panax ginseng and Panax quinquefolius L.By LC/MS/MS.Anal.Chem,1999,718,71(8):1579-1584.
[29]王宝琴.中药标准化回顾.中成药[J],2000,2(1):22-26.
[30]郑志华.加强中药质量标准研究是实现中药现代化的必要前提.中国药业[J].2001,10(2):4-6.
[31]李毓群.提高中药制剂质量的若干建议.中国中药杂志[J].2002.27(6):471-474.
[32]谢培山.中医药传统文化与现代质量控制[J]中国中西医结合杂志,1998,(11).
[33]Sticher O.Quality Of Ginkgo preparations.Planba Medica[J].1993,(59):2-11.
[34]谢培山.中药制剂色谱指纹图谱(图像)鉴别.中成药[J].2000,(6):391-395.
[35]邹汉法,汪海林.生物色谱技术分离、鉴定和筛选中药活性成分[J]世界科学技术,2000,(02).
[36]高夏红.中草药XRD指纹图谱方法学研究及中药XRD指纹图谱数据库建立[D]四川大学,2005
[37]王晃,李梦龙,覃洁萍,刘仲义.建立中药指纹图谱 保证中药产品质量[J]成都中医药大学学报,2002,(03).
[38]Wei W,Luo GA,Hua GY,et al.Capillary electrochromatographic separation of basic compounds with bare silica as stationary phase.J Chromatogr A,1998,8171-2,817(1-2):65.
[39]姚新生,胡柯.中药复方的现代化研究[J]化学进展,1999,(02).
[40]Romblum RN.Pituitary lession in emiocerebeal injuries[J].Aech.Path.1969,88,88:242.
Nei M.Estimation of Average Heterozygosity and Genetic Distancee from a Small Number of Individuals.Genetics,1978,89,89:583.
[41]Talapatra S K et al.On the chemistry of Indian Orchidaceae plants-Ⅱ.Dengibsin and dengibsinin,the first natural fluorenone derivatives from Dendrobium gibsonii Lindl.Tetrahedron,1985,4113,41(13):2765.
[42]Behr D et al.Studies on Orchidaceae glycosides part 6.Three steroid glycosides of the stigmastare type from Dendrobium ochreatum.Phytochemistry,1976,159,15(9):1403.
[43]葛恩慈.当前中药质量存在的问题及对策[J]浙江中医学院学报,1997,(03).
[44]苏薇薇,刘晓勇,吴忠.传统中药与中药现代化[J]中药材,2000,(08).
[45]M.Perrut,Advances in super critical fluid chromatography,J.Chromatogr.A,1994,658,293-313.
[46]董嘉德,超声技术在中药提取中的应用,中国药业,2002,11(11),55.
[47]杨景亮主编,《中医药工程研究与应用》,北京,中国中医药出版社.
[48]孙严彤.长白山花曲柳和水曲柳化学成份的研究:硕士学位论文.吉林,吉林大学,2004.