解毒活血汤治疗ⅢA型前列腺炎疗效观察及对大鼠前列腺组织TNF-α、IL-8、IL-10影响的实验研究
|
摘要
[目的]
1.观察解毒活血汤治疗ⅢA型前列腺炎的临床疗效及对前列腺液中TNF-α、IL-8、IL-10及WBC的影响,探求中医药治疗ⅢA型前列腺炎的作用机理并提供实验依据。
2.观察解毒活血汤对慢性非细菌性前列腺炎大鼠前列腺组织TNF-α、 IL-8、IL-10的影响。
[方法]
1.临床观察：按就诊顺序纳入80名ⅢA型慢性前列腺炎患者,随机分为治疗组(解毒活血汤组)和对照组(舍尼通组),每组40人。治疗组以解毒活血汤口服,每次1袋(150ml),日2次,早晚分服。对照组予以舍尼通片,每次1片,日2次,早晚分服。两组均以8周为一个疗程。所有患者受试前后均使用NIH-CPSI表进行症状评分和中医证侯症状评分。在受试前后,分别收集患者的前列腺液保存。所有受试者临床治疗前后使用EL ISA法检测前列腺液中的TNF-α、IL-8、IL-10含量。
2.动物实验：从40只雄性SD大鼠中随机抽取30只,去势后,经皮下多点注射苯甲酸雌二醇造成慢性非细菌性前列腺炎动物模型,剩余10只作为空白对照组(正常组)。造模成功后,慢性非细菌性前列腺炎模型大鼠再随机分成模型组、舍尼通组、解毒活血汤组。解毒活血汤组给药量为1g/kg·d(即4ml/d),舍尼通组给药量为0.025mg/kg·d(即4ml/d),正常组、模型组给予相同剂量的蒸馏水。以上各组均灌胃给药,每日一次,连续给药30d。治疗结束后将各组大鼠称重后处死。取前列腺组织,观察其病理改变,用ELISA法测定前列腺组织匀浆TNF-α、IL-8、IL-10的含量。
[结果]
1.临床研究：①解毒活血汤能有效治疗ⅢA型CPPS,能够明显改善患者临床症状,有效降低NIH-SCPI积分,能明显改善中医证侯症状积分,能够明显降低EPS中WBC含量。②解毒活血汤能够明显降低患者EPS中TNF-α、IL-8的水平。它对ⅢA型CPPS患者的局部免疫功能有调节作用。③EPS中TNF-α、IL-8、IL-10的含量与NIH-CPSI中疼痛症状积分呈正相关(p＜0.05)。
2.动物实验：①病理观察显示,模型组大鼠前列腺组织表现为慢性炎症的病理变化。其前列腺腺体普遍与周围组织粘连,腺体呈暗红色或棕黄色,质稍硬。显微镜下主要表现为淋巴细胞和单核细胞的浸润,间质水肿及纤维组织增生,前列腺上皮破坏、萎缩及脱落,前列腺管壁的增生或破坏。对前列腺病理切片的分级进行统计分析,显示模型组大鼠与正常大鼠前列腺组织病理表现差异有统计学意义(P＜0.01),表明造模成功。②解毒活血汤组大鼠前列腺上皮组织基本恢复正常,管壁结构完整,间质水肿消失。病理分级统计结果显示解毒活血汤组及舍尼通组与模型组比较,差异有统计学意义(P＜0.01,P＜0.05)。③模型组大鼠前列腺组织TNF-α、IL-8的水平较正常组升高(P＜0.01)；解毒活血汤组大鼠前列腺组织TNF-α、L-8的水平较模型组明显降低(P＜0.01),IL-10的水平较模型组差异无显著性意义(P＞0.05)。
[结论]
1.解毒活血汤能有效治疗ⅢA型CPPS,能够明显改善患者临床症状,有效降低NIH-SCPI积分,能明显改善中医证侯症状积分,能够明显降低EPS中WBC含量,消除炎症,减轻患者的病痛,是目前值得提倡的治疗方法和临证思维方法。
2.ⅢA型CPPS,疼痛症状积分与细胞因子TNF-α、IL-8、IL-10呈正相关,可作为ⅢA型CPPS病情和治疗效果制定的主要参考指标。
3.慢性非细菌性前列腺炎模型大鼠前列腺组织中TNF-α、IL-8、IL-10水平升高,说明细胞因子参与慢性非细菌性前列腺炎发病过程,通过对其调节,能够达到减轻或消除前列腺组织炎症反应,达到治疗作用。
4.解毒活血汤能够减轻或消除大鼠前列腺组织的炎症反应,修复其破坏的组织结构,对前列腺组织起到明显保护作用。为临床研究提供了实验依据。
5.解毒活血汤能够降低模型大鼠前列腺组织内促炎性因子TNF-α、 IL-8的水平,从而减轻前列腺组织炎症反应的程度。
Object:1. To explore the mechanism and provide experimental basis of traditional Chinese medicine's treatment in type Ⅲa prostatitis, on the basis of observing the curative effect of the Detoxicating and activating blood Decoction's treatment in type III A prostatitis and its influence inTNF-α、IL-8、 IL-10and WBC in prostatic fluid.
2. To observe the effect of Detoxicating and activating blood Decoction on TNF-α,IL-8,DL-10in rats prostate tissue with chronic nonbacterial prostatitis.
Methods:1. The clinical observation:Bring into80type ⅢA patients with chronic prostatitis according to treatment's sequence, divide into the treatment group (Detoxicating and activating blood Decoction group) and the control group (Cernilton group) randomly, n=40. The treatment group take Detoxicating and activating blood Decoction150ml each, twice per day, morning and evening hours service. The Control group take Cernilton slices each one for one pill and twice per day, morning and evening hours service. The two groups are in a course of treatment with eight weeks. All patients use the NIH-CPSI table symptom score before and after the test. Patients'prostatic fluid were collected and saved before and after the treatment.The content of TNF-α、 IL-8、DL-10in all subjects'prostatic fluid should be detected by ELISA before and after the clinical treatment.
2. Animal experiments:selected30male SD rats from the40ones randomly, castrated and injected subcutaneously with estradiol benzoate and caused to be the animal models of chronic non-bacterial prostatitis. The remaining10as the blank control group (normal group). After the modeling, the chronic non-bacterial prostatitis rat model were divided into model group, Cernilton group and Detoxicating and activating blood soup group randomly. The dosage of Detoxicating and activating blood soup group is1g/kg·d (4ml/d), the Cernilton group's is0.025mg/kg·d (4ml/d),the normal group and the model group were given the same dosage with distilled water. These groups were administered orally, once daily, administered for30d continuously. The rats were killed after weighting at the end of treatment. Take the prostates' issue to observe their pathological changes, measure the content of TNF-α、IL-8、IL-10in homogenates of prostates'tissue by ELISA.
Results:1. Clinical study:①Detoxicating and activating blood soup can treat type IIIA CPPS effectively, improve patients'clinical symptoms significantly, reduce the points of NIH-SCPI effectively, lessen the TCM syndrome score obviously, decrease the content of the WBC in EPS.②Detoxicating and activating blood soup can significantly reduce the levels of TNF-α IL-8in patients'EPS. It has the localized immune function regulation to type Ⅲa CPPS patients.③The levels of TNF-α、IL-8、IL-10in EPS and the score of pain symptom of the NIH-CPSI was correlated positively (p<0.05).
2. Animal experiments:①pathological observation show that the pathological changes of the prostate tissue of model group rats are chronic inflammation. The prostate gland generally adhere the surrounding tissue, glands are dark red or brown and its quality are a little stiff. Under the microscope,their performance are mainly lymphocytes and mononuclear cell infiltration, interstitial edema and fibrous tissue hyperplasia, prostate epithelial damage, atrophy and loss, prostate wall hyperplasia or destruction. The statistical analysis of classification of pathological slice of prostate grand biopsy show difference significantly (P<0.01) between model rats and normal rat indicate that the model was successful.②Detoxicating and activating blood soup group rats'prostate epithelial tissue returned to be normal, the wall structural integrity, interstitial edema disappeared. Pathological classification statistics show the difference was statistically significant (P<0.01, P<0.05) among the comparison of the Detoxicating and activating blood soup groups the Cernilton group and the model group.③The levels of TNF-a, IL-8in model group rats'prostate tissue have more increasing than the normal group's (P<0.01); the levels of TNF-a, IL-8in the rats'prostate tissue of the Detoxicating and activating blood soup group compared with the model group's were significantly lower (P<0.01),while the levels of EL-10compared with model group's don't have difference obviously (P>0.05).
Conclusion:
1.The treament of Detoxicating and activating blood Decoction can treat type III A CPPS effectively, improve patients'clinical symptoms significantly, reduce the points of NEH-SCPI effectively, lessen the TCM syndrome score obviously, decrease the content of the WBC in EPS, eliminate inflammation and reduce the patient's pain, which is a recommendable treatment method and Clinical Thoughts.
2.Type Ⅲa CPPS's scores of pain symptom and the cytokine of TNF-α、IL-8、 IL-10were positively related, which can be formulated as the reference index of patient's condition and treatment effective of type IIIA CPPS.
3. The levels of TNF-α、IL-8、IL-10in prostates'tissue of rat model with chronic non-bacterial prostatitis were increased, which indicate that the cytokines are involved in the incidence of inflammation of chronic non-bacterial prostatitis. We can achieve a therapeutic effect by the mean of its regulation which can alleviate or eliminate the inflammation in the prostates'tissue.
4.Detoxicating and activating blood Decoction can reduce or eliminate the inflammatory response of rat prostate tissue, improve the destruction of its organizational structure, which play a significant protective effect on prostate tissue and complement the experimental basis for clinical studies.
5. Detoxicating and activating blood Decoction can reduce the levels of promote inflammatory factor of TNF-a and IL-8in prostates'tissue of the rat model, thereby reducing the extent of inflammation in prostate tissue.
1.观察解毒活血汤治疗ⅢA型前列腺炎的临床疗效及对前列腺液中TNF-α、IL-8、IL-10及WBC的影响,探求中医药治疗ⅢA型前列腺炎的作用机理并提供实验依据。
2.观察解毒活血汤对慢性非细菌性前列腺炎大鼠前列腺组织TNF-α、 IL-8、IL-10的影响。
[方法]
1.临床观察：按就诊顺序纳入80名ⅢA型慢性前列腺炎患者,随机分为治疗组(解毒活血汤组)和对照组(舍尼通组),每组40人。治疗组以解毒活血汤口服,每次1袋(150ml),日2次,早晚分服。对照组予以舍尼通片,每次1片,日2次,早晚分服。两组均以8周为一个疗程。所有患者受试前后均使用NIH-CPSI表进行症状评分和中医证侯症状评分。在受试前后,分别收集患者的前列腺液保存。所有受试者临床治疗前后使用EL ISA法检测前列腺液中的TNF-α、IL-8、IL-10含量。
2.动物实验：从40只雄性SD大鼠中随机抽取30只,去势后,经皮下多点注射苯甲酸雌二醇造成慢性非细菌性前列腺炎动物模型,剩余10只作为空白对照组(正常组)。造模成功后,慢性非细菌性前列腺炎模型大鼠再随机分成模型组、舍尼通组、解毒活血汤组。解毒活血汤组给药量为1g/kg·d(即4ml/d),舍尼通组给药量为0.025mg/kg·d(即4ml/d),正常组、模型组给予相同剂量的蒸馏水。以上各组均灌胃给药,每日一次,连续给药30d。治疗结束后将各组大鼠称重后处死。取前列腺组织,观察其病理改变,用ELISA法测定前列腺组织匀浆TNF-α、IL-8、IL-10的含量。
[结果]
1.临床研究：①解毒活血汤能有效治疗ⅢA型CPPS,能够明显改善患者临床症状,有效降低NIH-SCPI积分,能明显改善中医证侯症状积分,能够明显降低EPS中WBC含量。②解毒活血汤能够明显降低患者EPS中TNF-α、IL-8的水平。它对ⅢA型CPPS患者的局部免疫功能有调节作用。③EPS中TNF-α、IL-8、IL-10的含量与NIH-CPSI中疼痛症状积分呈正相关(p＜0.05)。
2.动物实验：①病理观察显示,模型组大鼠前列腺组织表现为慢性炎症的病理变化。其前列腺腺体普遍与周围组织粘连,腺体呈暗红色或棕黄色,质稍硬。显微镜下主要表现为淋巴细胞和单核细胞的浸润,间质水肿及纤维组织增生,前列腺上皮破坏、萎缩及脱落,前列腺管壁的增生或破坏。对前列腺病理切片的分级进行统计分析,显示模型组大鼠与正常大鼠前列腺组织病理表现差异有统计学意义(P＜0.01),表明造模成功。②解毒活血汤组大鼠前列腺上皮组织基本恢复正常,管壁结构完整,间质水肿消失。病理分级统计结果显示解毒活血汤组及舍尼通组与模型组比较,差异有统计学意义(P＜0.01,P＜0.05)。③模型组大鼠前列腺组织TNF-α、IL-8的水平较正常组升高(P＜0.01)；解毒活血汤组大鼠前列腺组织TNF-α、L-8的水平较模型组明显降低(P＜0.01),IL-10的水平较模型组差异无显著性意义(P＞0.05)。
[结论]
1.解毒活血汤能有效治疗ⅢA型CPPS,能够明显改善患者临床症状,有效降低NIH-SCPI积分,能明显改善中医证侯症状积分,能够明显降低EPS中WBC含量,消除炎症,减轻患者的病痛,是目前值得提倡的治疗方法和临证思维方法。
2.ⅢA型CPPS,疼痛症状积分与细胞因子TNF-α、IL-8、IL-10呈正相关,可作为ⅢA型CPPS病情和治疗效果制定的主要参考指标。
3.慢性非细菌性前列腺炎模型大鼠前列腺组织中TNF-α、IL-8、IL-10水平升高,说明细胞因子参与慢性非细菌性前列腺炎发病过程,通过对其调节,能够达到减轻或消除前列腺组织炎症反应,达到治疗作用。
4.解毒活血汤能够减轻或消除大鼠前列腺组织的炎症反应,修复其破坏的组织结构,对前列腺组织起到明显保护作用。为临床研究提供了实验依据。
5.解毒活血汤能够降低模型大鼠前列腺组织内促炎性因子TNF-α、 IL-8的水平,从而减轻前列腺组织炎症反应的程度。
Object:1. To explore the mechanism and provide experimental basis of traditional Chinese medicine's treatment in type Ⅲa prostatitis, on the basis of observing the curative effect of the Detoxicating and activating blood Decoction's treatment in type III A prostatitis and its influence inTNF-α、IL-8、 IL-10and WBC in prostatic fluid.
2. To observe the effect of Detoxicating and activating blood Decoction on TNF-α,IL-8,DL-10in rats prostate tissue with chronic nonbacterial prostatitis.
Methods:1. The clinical observation:Bring into80type ⅢA patients with chronic prostatitis according to treatment's sequence, divide into the treatment group (Detoxicating and activating blood Decoction group) and the control group (Cernilton group) randomly, n=40. The treatment group take Detoxicating and activating blood Decoction150ml each, twice per day, morning and evening hours service. The Control group take Cernilton slices each one for one pill and twice per day, morning and evening hours service. The two groups are in a course of treatment with eight weeks. All patients use the NIH-CPSI table symptom score before and after the test. Patients'prostatic fluid were collected and saved before and after the treatment.The content of TNF-α、 IL-8、DL-10in all subjects'prostatic fluid should be detected by ELISA before and after the clinical treatment.
2. Animal experiments:selected30male SD rats from the40ones randomly, castrated and injected subcutaneously with estradiol benzoate and caused to be the animal models of chronic non-bacterial prostatitis. The remaining10as the blank control group (normal group). After the modeling, the chronic non-bacterial prostatitis rat model were divided into model group, Cernilton group and Detoxicating and activating blood soup group randomly. The dosage of Detoxicating and activating blood soup group is1g/kg·d (4ml/d), the Cernilton group's is0.025mg/kg·d (4ml/d),the normal group and the model group were given the same dosage with distilled water. These groups were administered orally, once daily, administered for30d continuously. The rats were killed after weighting at the end of treatment. Take the prostates' issue to observe their pathological changes, measure the content of TNF-α、IL-8、IL-10in homogenates of prostates'tissue by ELISA.
Results:1. Clinical study:①Detoxicating and activating blood soup can treat type IIIA CPPS effectively, improve patients'clinical symptoms significantly, reduce the points of NIH-SCPI effectively, lessen the TCM syndrome score obviously, decrease the content of the WBC in EPS.②Detoxicating and activating blood soup can significantly reduce the levels of TNF-α IL-8in patients'EPS. It has the localized immune function regulation to type Ⅲa CPPS patients.③The levels of TNF-α、IL-8、IL-10in EPS and the score of pain symptom of the NIH-CPSI was correlated positively (p<0.05).
2. Animal experiments:①pathological observation show that the pathological changes of the prostate tissue of model group rats are chronic inflammation. The prostate gland generally adhere the surrounding tissue, glands are dark red or brown and its quality are a little stiff. Under the microscope,their performance are mainly lymphocytes and mononuclear cell infiltration, interstitial edema and fibrous tissue hyperplasia, prostate epithelial damage, atrophy and loss, prostate wall hyperplasia or destruction. The statistical analysis of classification of pathological slice of prostate grand biopsy show difference significantly (P<0.01) between model rats and normal rat indicate that the model was successful.②Detoxicating and activating blood soup group rats'prostate epithelial tissue returned to be normal, the wall structural integrity, interstitial edema disappeared. Pathological classification statistics show the difference was statistically significant (P<0.01, P<0.05) among the comparison of the Detoxicating and activating blood soup groups the Cernilton group and the model group.③The levels of TNF-a, IL-8in model group rats'prostate tissue have more increasing than the normal group's (P<0.01); the levels of TNF-a, IL-8in the rats'prostate tissue of the Detoxicating and activating blood soup group compared with the model group's were significantly lower (P<0.01),while the levels of EL-10compared with model group's don't have difference obviously (P>0.05).
Conclusion:
1.The treament of Detoxicating and activating blood Decoction can treat type III A CPPS effectively, improve patients'clinical symptoms significantly, reduce the points of NEH-SCPI effectively, lessen the TCM syndrome score obviously, decrease the content of the WBC in EPS, eliminate inflammation and reduce the patient's pain, which is a recommendable treatment method and Clinical Thoughts.
2.Type Ⅲa CPPS's scores of pain symptom and the cytokine of TNF-α、IL-8、 IL-10were positively related, which can be formulated as the reference index of patient's condition and treatment effective of type IIIA CPPS.
3. The levels of TNF-α、IL-8、IL-10in prostates'tissue of rat model with chronic non-bacterial prostatitis were increased, which indicate that the cytokines are involved in the incidence of inflammation of chronic non-bacterial prostatitis. We can achieve a therapeutic effect by the mean of its regulation which can alleviate or eliminate the inflammation in the prostates'tissue.
4.Detoxicating and activating blood Decoction can reduce or eliminate the inflammatory response of rat prostate tissue, improve the destruction of its organizational structure, which play a significant protective effect on prostate tissue and complement the experimental basis for clinical studies.
5. Detoxicating and activating blood Decoction can reduce the levels of promote inflammatory factor of TNF-a and IL-8in prostates'tissue of the rat model, thereby reducing the extent of inflammation in prostate tissue.
引文
1. Robertson C,Boyle P,Nonis A,etal.International population based studu of urological conditions:the Urepik study.Ⅱ.Comparative prostatitis data [J].Jurol,1999,161(4):32.
2.Wenninger K,Heiman J R,Rothman I,etal.Sickness impact of chronic nonbacterial prostatitis and its correlates [J]. J Urol,1996,155(3):965-968.
3.郭应禄,胡礼泉主编.男科学[M].北京：人民卫生出版社.2004：1485.
4.Razumov SV, Medvedev AA, Chirun NV,etal.Role of cytokines in the diagnosis of chronic prostatitis [J].Urologiia.2003,Nov-Dec(6):25-28.
5.Alexander RB,Ponniah,Hasday,etal.Elevated levels of proinflammatory cytokines in the semen of patients with chronic prostatitis.chronic pelvic pain syndrome [J].Urology.1998,52:744-749.
6.Dinaarello CA(2000).Proinflammatorycytokines[J].Chest.118:503-508.
7.Steven M.Opal,VeraA.DePalo,(2000).Anti-Inflammatory Cytokines [J]. Chest,117:1162-1172.
8.Hochreiter WW, Hruz P, Danuser H, Weidner W,Studer UE.Evaluation of symptoms in men with chronic pelvic pain[J].Prostate,2002,21(6):69-74.
9.陆德铭主编.中医外科学[M].上海：上海科学技术出版社.1997：204.
10.王万春,严张仁.解毒活血汤治疗慢性非细菌性前列腺炎的疗效观察[J].中华男科学杂志,2007,13(3)：275-276.
11.吴在德,吴肇汉.《外科学》.人民卫生出版社.2008年第七版：660-661.
12.那彦群,孙光.《中国泌尿外科疾病》.人民卫生出版社.2009,4-5.
13.国家中医药管理局.中华人民共和国中医药行业标准·中医病证诊断疗效标准.第一版.南京：南京大学出版社,1994：50.
14.中国中西医结合学会男科专业委员会.慢性前列腺炎中西医结合诊疗指南[J].中国中西医结合杂志,2007,27(11)：1052-1056.
15.郑筱萸.中医药新药临床研究指导原则(试行).北京：中国医药科技出版社,2002,112.
16.Nuckel JC, Nyberg LM. Hennenfet Ml. Recearch guidelines for chronic prostatis:consensus report from the first national institutes of health international prostatitis collaborative network Urology 1999; 54:229.
17.贾玉森,李曰庆,孙明杰等.前列腺炎栓治疗非特异性慢性前列腺炎(湿热夹瘀证)104例临床与实验研究[J].中医杂志,1999,40(2)：98-99.
18.国家中医药管理局.中华人民共和国中医药行业标准·中医病证诊断疗效标准.第一版.南京：南京大学出版社,1994：50.
19.顾方六主编.现代前列腺病学[M].第一版.北京：人民军医出版社,2002：521-593.
20.李兰群,王传航,刘春英等.慢性前列腺炎中医证型分布频率研究[J].中华中医药杂志,2005,20(4)：245-246.
21.于洪波,孙则禹.尿动力学异常与慢性前列腺炎诊治关系的探讨[J].中华男科学杂志,2001,7(4)：240-242.
22.Zabokowski T,Peterek J,S RAWAZR b.The results of treament of chronic prostatitis on the background of anaerobic bacterial and fugal infection[J].Wiad Lek,1999,52:456-461.
23.毛普德,王建军.衣原体与支原体性前列腺炎的诊断和治疗[J].四川医学,2002,23(10)：1013-1014.
24.梁铭,林有坤.慢性前列腺炎患者滴虫感染情况分析[J].中国麻风皮肤病杂志,2003,19(5)：461-462.
25.张亚强.活血化瘀是治疗慢性前列腺炎的有效治则和方法[J].中国中西医结合杂志,2002,22(3)：218-219.
26.张亚强.前列腺方治疗前列腺炎血瘀证的临床与实验研究[J].中国中西结合杂志,1998,18(9)：534-536.
27. Roberts RO, LieberMM, Bostwick DG, etal. Areview of clinical and pathological prostatitis syndromes[J]. Urology,1997,49(6):809-821.
28.唐孝达.慢性前列腺炎.慢性盆腔疼痛综合征诊治中值得商榷的问题[J].中华男科学杂志,2003,9(6)：403-406.
29.周晓辉,周智恒,刘忠德.自身免疫因素导致慢性非细菌性前列腺炎的研究进展[J].中国中西医结合外科杂志,2003,9(3)：241-243.
30.Hoehreiter ww, Nadler RB, Koch AE, etal. Evaluation of the eytokines interleukin 8 and epithelial neutrophil aetivating peptide 78 as indicators of inflammation in prostatic seretions[J]. Urology,2000, 56(6):1025-1029.
31.周晓辉,韩蕾,周智恒等.免疫性慢性非细菌性前列腺炎大鼠模型的形态学与分子生物学特性[J].中华男科学杂志,2005,11(4)：290-295.
32.Sehaeffer AJ, Knauss JS, Landis JR, etal. Leukocyte and bacterial counts do not correlate with severity of symptoms in men with chronic prostatitis:the National Institutes of Health Chronic Prostatitis Cohort Study[J]. JUrol,2002,168:1048-1053.
33.唐孝达.慢性前列腺炎诊断与治疗进展[J].中国男科学杂志,2002,16(3)：193-196.
34.Shortliffe LM,Wehner N.The characterization of bacterial and nonbacterial proststitis by prostatic immunoglobulins.Medicine.1986,Nov,65(6):399-414.
35.Alexander RB,Ponniah,Hasday,etal.Elevate levels of proinflammatory cytokines in the semen of patients with chronic prostatitis.chronic pelvic pain syndrome[J].Urology.1998,52:744-749.
36.Batstone GR,Doble A,Gaston JS.Autoimmune T cell responses to seminal plasma in chronic pelvic pain inflammation(CPPS)[J].Clinical and Experimental mmunology,2002,128(2):302-303.
37.Ponniah S,Arah I,Alexander RB. PSA is a candidate self-antigen in autoimmune chronic prostatitis/ chronic pelvic pain syndrome[J]. Prostate,2000,44(1):49-50.
38.李树平,孟双艳等.慢性非细菌性前列腺炎患者血清和前列腺液4种细胞因子检测及意义[J]中华男科学杂志,2006,1：25-27.
39.李树平,孟双艳等.慢性前列腺炎患者血清细胞因子的水平变化及其意义[J]医学理论与实践,2006,3：266-267.
40.汪岩.赵忠文,王立波.前列腺液中细胞因子(IL-6、TNF-a)在慢性前列腺炎中的意义[J].自求恩医科大学学报,1999.25(4)：402.
41.孙一呜,刘丽.前列腺水丸对慢性细菌性前列腺炎大鼠血清IL-6和TNF-a水平的影响[J].中华男科学杂志,2006,5：470-472.
42.吴永吉,刘硕.慢性非细菌性前列腺炎症状与前列腺液中白介素一10水平的关系[J].医师进修杂志2005,28(10：37-38.
43.The efects of interleukin-10 and-8 in chronic prostatitis. Zhong Hua Nan Ke Xue。2004,10(7):486-490.
44.John H,Barghom A,Funke G,etal. Noninflammatory chronic pelvicpain syndrome:immunological study in blood,ejaculate and prostate tissue [J].Euro Urol,2001,39:72-78.
45.赵海军,韩保健,侯成玉.慢性非细菌性前列腺炎患者前列腺液中TNF-α、IL-10水平变化及意义[J].山东医药2007,47(21)：52-53.
46.Miller LJ, Fischer KA, Goralnick SJ. elaf. Interleukin.10 lcvels in seminal plasma:implications for chronic prostatitis-chronic pelvic pain syndrome[J]. JUrol,2002,167(2 Pt 1):753-756.
47.刘广省.土茯苓的药理研究进展[J].中药材,2001.24(8)：615-616.
48.芦绪芳.金银花的研究现状[J].时珍国医国药,2006,17(5)：843-844.
49李雪征,金光洙.萆薢的研究进展[J].中国野生植物资源,2002,21(5)：8-10.
50.邱静帆,殷颖.车前子的药学研究及其应用[J].浙江中西医结合杂志,2002,12(1)：55.
51.吕刚,李婷婷.石菖蒲的研究进展[J].时珍国医国药,2006,17(7)：1291-1292.
52.徐富一,金正子,申舜植.王不留行与硬叶女娄菜止痛、消炎效能的比较研究[J].河南中医学院学报,2006,21(2)：31-32.
53.马丽.穿山甲的药理及临床研究[J].中医药研究,2002.18(2)：46-47.
54.伍晓春,陆豫.虎杖的药理作用及临床应用研究进展[J].中医药信息,2005.22(2)：22-25.
55.赵兴梅,徐光忠,李建利等.川牛膝和怀牛膝的现代药理研究概况[J].华西药学杂志,2004,19(3)：205-207.
56.吕清文,丛雅勤.延胡索药理作用及临床应用浅议[J].内蒙古中医药,2005,01(01)：26.
57.史桂兰,胡志浩.甘草酸药理作用及临床应用研究进展[J].天津市医药科学杂志,2003,4(9)：11-12.
58..Loschen G. Ebeling L. Inhibition of the arachidonatc metabolism by an extract from rye pollen[J]. Drug Res.1991,41:162-167.
59.张亚强,卢建新,吴志奎等.丹蒲胶囊对慢性前列腺炎患者前列腺液IL-2、IL-6水平的影响[J].中国中西医结合杂志,2002.22(11)：828-829.
60.魏武然,张唯力,戴君勇.大鼠慢性非细菌性前列腺炎模型的建立.中国男科学杂志,2006,20(1)：222-224
61.徐叔云,卞如濂,陈修主编.药理实验方法学.第3版.北京：人民卫生出版社,2002：1557：911
62.Lang MD.Nickel C,Olson ME,et al.Rat model of experimentally induced abactedal pmstatitis[J]. Prostate 2000,45:201.
63.戴苏林,朱进,魏秀德,等.前列腺康栓对大鼠实验性非细菌性前列腺炎的抑制作用.中成药.1990,12(8)：27-28.
64.张亚强,刘猷枋.中药前列腺汤对实验性前列腺炎病理模型的影响.中国中西医结合杂志.1991,11(8)：480-482.
65.唐风珍,侯蕙茹,张景云,等.大鼠前列腺炎的制备方法.中国药理学通报.1998,14(2)：187-188.
66.Harritl MT Feldberg RS, Lau KM et aL. Exlxessing of proinflamatory genes during estrogen-induced inflammation of the rat prorate[J].Prorate 2000,44:19.
67.Michael J. Wlison,Mildced Woodson, Carol Wiehr,et al. Matrix metalloproteinases in the pathogencsis of estradiol-induced nonbactefial prostatitis in the lateral prostate lobe of the Wistar rat. Experimental and molecular Pathology [J].2004:77:7-17.
68. Kwon SM, Kim SI, Chun DC, et al. Development of rat prostatitis model by oral administration of isoflavone and its characteristics[J], Yonsei Med J,2001,42(4):395-404.
69.叶伟成,薛慈民,徐兆东等.免疫佐剂法制作慢性非细菌性前列腺炎小鼠模型的方法.中国男科学杂志2001.15(1)：29.
70. Keetch DW, Humphrey P, Ratliff TL. Development of a mouse model for nonbacterial prostatitis[J]. J Urol,1994,152(1):247-250.
71.Paulis G, Conti E, Voliani S, Bertozzi MA, et al. Evaluation of the cytokines in genital secretions of patients with chronic prostatitis[J]. Arch Ital Urol Androl,2003,75(4):179-186.
72.张庆云,莫曾南,刘硒碲.姜黄素对慢性非细菌性前列腺炎大鼠TNF-α、TL-6和IL-8表达的影响.中华男科学杂志,2010,16(1)：84-88.
73.王洪志,刘朝东,韦超IFN-γ、TNF-a和IL-10在慢性非细菌性前列腺炎大鼠中的表达及意义.重庆医科大学学报,2009,34(9)：1187-1190.
74.Miller LJ, Fischer KA, Goralnick SJ, et aL. Interleukin-10 levels in seminal plasma:implications for chronic prostatitis-chronic pelvic pain syndrome[J]. J Urol,2002,167(2):753-756.
75.赵海军,韩保健,侯成玉.慢性非细菌性前列腺炎患者前列腺液中TNF-α、IL-10水平变化及意义.山东医药,2007(21)：52-53.
[1]Robertson C, Boyle P, Nonis A,etal.International population based studu of urological conditions:the Urepik study. II.Comparative prostatitis data [J].Jurol,1999,161(4):32.
[2]Wenninger K, Heiman J R, Rothman I, etal. Sickness impact of chronic nonbacterial prostatitis and its correlates [J]. J Urol,1996,155(3):965-968.
[3]郭应禄,胡礼泉主编.男科学[M].北京：人民卫生出版社.2004：1485.
[4]郭霭春.黄帝内经素问校注[M].北京：人民军医出版社,1992年,第一版：571.
[5]李用粹.证治汇补[M].北京：科技卫生出版社,卷八：441.
[6]张景岳.景岳全书[M].北京：人民卫生出版社,1991年,第一版：640.
[7]伊广迁,李永占.明清十八家名医医案[M].北京：中国中医药出版社,1997：325-326.
[8]沈时誉.医衡[M].上海：上海书店,1985：67.
[9]程仲龄.医学心悟[M].北京：科学技术文献出版社,1996,第一版：159.
[10]谢映卢.得心集医案.珍本医学集成本[M].上海：世界书局,1936,112.
[11]叶天士.临证指南医案[M].北京：华夏出版社,1995：303-307.
[12]巢元方.诸病源候论[M].北京：人民卫生出版社,1982：128.
[13]林佩琴.类证治裁[M].北京：中国中医药出版社,1997年,第一版：197.
[14]徐春甫.古今医统大全[M].北京：人民卫生出版社,1991年：965.
[15]张锡纯.医学衷中参西录[M].石家庄：河北科技出版社,1985年：487.
[16]何梦瑶.医碥[M].上海：上海科学技术出版社,1982年,第一版：232.
[17]李兰群,周强.李曰庆论治慢性前列腺炎经验[J].山东中医杂志2002.12(12)：745-746.
[18]吴少刚,王琦治疗慢性前列腺炎的临床思维[J].山东中医杂志1994,13(10)：438-439.
[19]王天明.慢性前列腺炎的辨治体会[J].河南中医药学刊,2002,17(6)：64-65.
[20]张耀圣.谈前列腺炎复发的预防[J].光明中医,200217(4)：23-24.
[21]徐福松.辨证与辨病论治慢性前列腺炎[J].男科学报1999,5(1)：6-11.
[22]龚翰林、邹飞、李俊涛等.王久源教授辨治慢性前列腺炎临床经验[J].四川中医2005,23(3)：6-7.
[23]唐学会.郭军教授治疗慢性前列腺炎经验[J].河南中医2007,27(4)：15-16.
[24]石志超.从血瘀精道论治慢性前列腺炎[J].辽宁中医杂志,2001,28(11)：663-664.
[25]李承功.周智恒治疗前列腺疾病经验[J].江西中医药2002,33(2)：5.
[26]陆德铭主编.中医外科学[M].上海：上海科学技术出版社,1997：204-205.
[27]刘建邦,徐永明.辨证治疗慢性前列腺炎32例体会[J].新疆中医药,2002,20(1)：18.
[28]刘宁飞.辨证治疗慢性前列腺炎50例[J].湖南中医药导报,1997,3(4)：53.
[29]邹方伟,姜兆友,霍振国.辨证施治慢性前列腺炎102例[J].山东中医杂志,2003,22(5)：285.
[30]彭煜.泉宁方治疗非细菌性前列腺炎的临床观察[J].上海中医药杂志,2004,38(5)：35-36.
[31]王权胜,唐振宇.程氏萆藓分清饮治疗慢性前列腺炎40例效果观察[J].现代中西医结合杂志,2004,13(12)：1571-1572.
[32]叶志雄,谢长才.黄柏前列汤治疗慢性非细菌性前列腺炎的临床观察[J].湖北中医杂志,2005,27(6)：27-28.
[33]徐新建,周智恒.清热化瘀法治疗慢性非细菌性前列腺炎80例[J].中国中西医结合外科杂志.2005.11(3)：243-244.
[34]张朝德.疏肝通络法治疗慢性非细菌性前列腺炎疗效观察[J].吉林中医药,2004.24(9)：34-35.
[35]高洪寿,任平.从肝论治慢性非细菌性前列腺炎的疗效观察[J].中国男科学杂志,2002.16(4)：332-333.
[36]曾晔,赖海标,钟亮等.坐盆汤坐浴治疗慢性非细菌性前列腺炎的临床观察[J].中医外治杂志,2005.14(2)：16-17.
[37]孙天成,李君森,程美.六味地黄汤加味配合温热水坐浴治疗慢性无菌性前列腺炎[J].
中国临床医药研究杂志,2007(173：44.
[38]崔飞婵,苏斌,蔡东.中药灌肠加外敷治疗慢性非细菌性前列腺炎52例[J].新中医,2004.36(7)：67-68.
[39]罗建华,张西芝.“盆腔灵”直肠灌注治疗慢性非细菌性前列腺炎疗效观察[J].男科学报,1998.4(1)：62-63.
[40]陈其华,席建元.五圣消炎栓直肠给药治疗慢性前列腺炎90例[J].中国中医药信息杂志,2003.10(10)：53-54
[41]王尉,何恢绪,胡卫列等.前列腺内直接注射川参通治疗慢性非细菌性前列腺炎／慢性骨盆疼痛综合征[J].中华男科学杂志,2004.10(3)：182-187.
[42]刘紫庭,廖海球,杨菊云.川参通直接注入前列腺治疗慢性非细菌性前列腺炎／慢性盆腔疼痛综合征[J].中华男科学杂志,2005.11(9)：706-707.
[43]薄立宏.双黄连粉针剂膀胱内灌注治疗慢性前列腺炎18例[J].中医研究,1997.10(4)：34-35.
[44]吴玉清,李承功.中药口服合双黄连尿道灌注治疗慢性前列腺炎60例[J].中国民间疗法,2002.10(2)：24-25.
[45]于峰.自我按摩治疗慢性前列腺炎45例[J].按摩与导引,1997.3：42
[46]郑文华.清利化瘀汤加前列腺按摩治疗慢性非细菌性前列腺炎96例[J].广西中医学院学报,2006.9(2)：39-40.
[47]刘继能.自然特异功治疗慢性前列腺36例[J].气功与科学,1994.4：19.
[48]何天有,赵耀东,雒成林.针刺“三阴穴”治疗慢性前列腺炎临床观察[J].中国针灸,2004,24(10)：697—698.
[49]何馨.针灸治疗慢性前列腺炎36例临证观察[J].中医药学刊,2001.19(4)：387.
[50]葛继魁,葛书翰.深刺白环俞为主治疗慢性前列腺炎临床观察[J].中国针灸,2001,21(2)：73.
[51]孙自学,吴宏东.芒针治疗慢性非细菌性前列腺炎的临床研究[J].中医药学刊,2003.21(1)：77-78.
[52]张培永,王霄鹏.艾灸会阴与坐浴对照治疗非细菌性前列腺炎90例[J].中医外治杂志,1999.8(5)：13-14.
[53]陈昭典.前列腺炎综合征的历史演变与进展[J].中国男科学杂志,2003.17(6)：633-368.
[54]Drach GW, Fair WR, Meates EM. etal. Classification of benign diseases associated with prostatic Pain:prostatitis and prostatodynia[J]. Jurol 1978:120-266
[55]Krieger JN. Nyberg LJr, Nickel JC. NIH consensus definition and classification of prostatitis[J]. JAMA 1999.21:282(3):236-237。
[56]石理华,李瑛,李辉等.诊断为慢性非细菌性前列腺炎患者的前列腺液和前列腺组织中细菌基因的检测[J].武警医学报,2007.16(1)：26-27
[57]Nickel JC.Prostatitis:Evolving and management strategies[J].Urol clin North AM,1999,26(4):737-751
[58]Shoskes DA, Albakri Q, Thomas K, etal[J]. JUrol 2002; 168(1):331-335
[59]邓春华,梁宏,梅骅.前列腺内尿液返流在慢性前列腺炎发病中的作用[J].中华泌尿外科杂 志,1998,19(6)：288-298.
[60]陈琦,魏莎莉.慢性非细菌性前列腺炎／慢性盆腔疼痛综合征发病机制研究进展.国外医学泌尿系统分册,2005,25(5)：629
[61]谢辉,杨亦荣.前列腺痛患者情绪障碍的分析及治疗[J].临床泌尿外科杂志,2002,17(6)：288-289
[62]徐英民,高维忠,夏术阶等.普拉宗与泌尿灵联用治疗慢性非细菌性前列腺炎和前列腺痛的疗效观察[J].实用男科杂志,1996,2(1)：21-23.
[63]埃杜,莫曾南.特拉唑嗪治疗慢性前列腺炎／慢性盆腔疼痛综合征的系统评价[J].广西医学,2007,29(6)：789-792.
[64]ItoR, IshiM, YamaahitaS,etal. Antiprostatic hypertrophic action of Cernihon. pollen extract[J]. Pharmacometrics,1986.3:1-11.
[65]阎洪涛,廖勇,龚百生等.舍尼通治疗ⅢA型前列腺炎的临床疗效[J].中华男科学杂志,2004,10(12)：930-931.
[66]叶章群,蓝儒竹,王少刚等.舍尼通和抗生素联合用药治疗慢性非细菌性前列腺炎临床研究[J].中华男科学杂志,2006,12(9)：807-810.
[67]Persson BE,Ronquist G,EKb lomM. Ameliorative efect of allop urinol on nonbacterial prostatitis:a parallel double-blind controlled study[J].J Urol.l996.155(3):961-964.
[68]张培升,陈琴.抗菌药对慢性非细菌性前列腺炎的疗效观察[J].海峡药学,2001,13(3)：58-59.
[69]许明,张永康.抗菌药对慢性非细菌性前列腺炎的治疗作用[J].临床泌尿外科杂志,2000,15(5)：195-196.
[70]范华,甘肖余.慢性前列腺炎122例治疗体会[J].广西医学,2005,27(3)：416-417.
[71]蒋建光.经皮(会阴)穿刺行药物注射治疗慢性前列腺炎1640例[J].实用中西医结合临床,2007,7(4)：6-7.
[72]杨振玲, 卜宏民.骶管阻滞治疗慢性前列腺炎80例临床研究[J].河南外科学杂志,2005,11(5)：66.
[73]ZHOU Zhan-song,SONG Be,NIE Fa-chuan,et al.Treatment of intractable chronic pelvic pain syndrome by injecting a compound of Bupivacaine and Fentanyl into sacral spinal space[J].Journal of Medical Colleges of PLA.2006,21(4):258-260.
[74]黄慧,龙媛媛,柳青.尿道内药物灌注治疗非细菌性前列腺炎的观察[J].中国民康医学杂志.2004,16(11)：692。
[75]王爱民,杨斌,刘巧等.体外高频热疗治疗慢性前列腺炎临床疗效观察[J].临床皮肤科杂志,2004,33(12)：764
[76]林芝,黎承阳.射频热疗治疗慢性前列腺炎36例总结[J].右江医学杂志,1998,26(2)：77-78
[77]Nickel JC, Downey J, Feliciano AE, etal. Repetitive prostatic massagetherapy for chronic refractory pro statitis:the phililppin e expe rievee[J].TechUrol,1999,5(3):146-151
[78]周巧奇,朱湘生.前列腺按摩配合特拉唑嗪治疗慢性前列腺炎86例临床观察[J].中国性科学,2007,16(1)：22-23
[79]胡跃刚.慢性前列腺的药物综合治疗临床观察[J].中国医院药学杂志,2006,26(11)：1386-1387
[80]游秀英,宋万蝉.双囊三腔管前列腺药物灌注治疗非淋菌性前列腺炎100例临床分析[J].右江民族医学院学报,2006,6：996-997.
[81]倪颖,朱晓群,江汉群.综合心理、行为干预治疗慢性非细菌性前列腺炎效果观察[J].中国行为 医学科学,2002,11(6)：656-657.
[82]戴胜国,冷静,吕坚伟.中西医结合综合治疗慢性前列腺炎2284例分析[J].中国男科学杂志,2002,16(41)：302-304
[83]郑海滨,崔艳荣.综合疗法治疗慢性前列腺炎临床应用研究[J].中华男科学杂志.2004,10(9)：701-703
[84]吕建林.中西医结合治疗慢性前列腺炎150例疗效观察[J].新中医,2003,35(1)：42-43
[85]江先汉,郭燕东,张文海等.中西医结合治疗慢性前列腺炎的临床总结[J].现代医药卫生,2004,20(12)：1074-1075
[86]李云武,陈金雄,郑清水.中西医结合治疗慢性前列腺炎60例[J].实用中医药杂志,2002,18：30—31.
2.Wenninger K,Heiman J R,Rothman I,etal.Sickness impact of chronic nonbacterial prostatitis and its correlates [J]. J Urol,1996,155(3):965-968.
3.郭应禄,胡礼泉主编.男科学[M].北京：人民卫生出版社.2004：1485.
4.Razumov SV, Medvedev AA, Chirun NV,etal.Role of cytokines in the diagnosis of chronic prostatitis [J].Urologiia.2003,Nov-Dec(6):25-28.
5.Alexander RB,Ponniah,Hasday,etal.Elevated levels of proinflammatory cytokines in the semen of patients with chronic prostatitis.chronic pelvic pain syndrome [J].Urology.1998,52:744-749.
6.Dinaarello CA(2000).Proinflammatorycytokines[J].Chest.118:503-508.
7.Steven M.Opal,VeraA.DePalo,(2000).Anti-Inflammatory Cytokines [J]. Chest,117:1162-1172.
8.Hochreiter WW, Hruz P, Danuser H, Weidner W,Studer UE.Evaluation of symptoms in men with chronic pelvic pain[J].Prostate,2002,21(6):69-74.
9.陆德铭主编.中医外科学[M].上海：上海科学技术出版社.1997：204.
10.王万春,严张仁.解毒活血汤治疗慢性非细菌性前列腺炎的疗效观察[J].中华男科学杂志,2007,13(3)：275-276.
11.吴在德,吴肇汉.《外科学》.人民卫生出版社.2008年第七版：660-661.
12.那彦群,孙光.《中国泌尿外科疾病》.人民卫生出版社.2009,4-5.
13.国家中医药管理局.中华人民共和国中医药行业标准·中医病证诊断疗效标准.第一版.南京：南京大学出版社,1994：50.
14.中国中西医结合学会男科专业委员会.慢性前列腺炎中西医结合诊疗指南[J].中国中西医结合杂志,2007,27(11)：1052-1056.
15.郑筱萸.中医药新药临床研究指导原则(试行).北京：中国医药科技出版社,2002,112.
16.Nuckel JC, Nyberg LM. Hennenfet Ml. Recearch guidelines for chronic prostatis:consensus report from the first national institutes of health international prostatitis collaborative network Urology 1999; 54:229.
17.贾玉森,李曰庆,孙明杰等.前列腺炎栓治疗非特异性慢性前列腺炎(湿热夹瘀证)104例临床与实验研究[J].中医杂志,1999,40(2)：98-99.
18.国家中医药管理局.中华人民共和国中医药行业标准·中医病证诊断疗效标准.第一版.南京：南京大学出版社,1994：50.
19.顾方六主编.现代前列腺病学[M].第一版.北京：人民军医出版社,2002：521-593.
20.李兰群,王传航,刘春英等.慢性前列腺炎中医证型分布频率研究[J].中华中医药杂志,2005,20(4)：245-246.
21.于洪波,孙则禹.尿动力学异常与慢性前列腺炎诊治关系的探讨[J].中华男科学杂志,2001,7(4)：240-242.
22.Zabokowski T,Peterek J,S RAWAZR b.The results of treament of chronic prostatitis on the background of anaerobic bacterial and fugal infection[J].Wiad Lek,1999,52:456-461.
23.毛普德,王建军.衣原体与支原体性前列腺炎的诊断和治疗[J].四川医学,2002,23(10)：1013-1014.
24.梁铭,林有坤.慢性前列腺炎患者滴虫感染情况分析[J].中国麻风皮肤病杂志,2003,19(5)：461-462.
25.张亚强.活血化瘀是治疗慢性前列腺炎的有效治则和方法[J].中国中西医结合杂志,2002,22(3)：218-219.
26.张亚强.前列腺方治疗前列腺炎血瘀证的临床与实验研究[J].中国中西结合杂志,1998,18(9)：534-536.
27. Roberts RO, LieberMM, Bostwick DG, etal. Areview of clinical and pathological prostatitis syndromes[J]. Urology,1997,49(6):809-821.
28.唐孝达.慢性前列腺炎.慢性盆腔疼痛综合征诊治中值得商榷的问题[J].中华男科学杂志,2003,9(6)：403-406.
29.周晓辉,周智恒,刘忠德.自身免疫因素导致慢性非细菌性前列腺炎的研究进展[J].中国中西医结合外科杂志,2003,9(3)：241-243.
30.Hoehreiter ww, Nadler RB, Koch AE, etal. Evaluation of the eytokines interleukin 8 and epithelial neutrophil aetivating peptide 78 as indicators of inflammation in prostatic seretions[J]. Urology,2000, 56(6):1025-1029.
31.周晓辉,韩蕾,周智恒等.免疫性慢性非细菌性前列腺炎大鼠模型的形态学与分子生物学特性[J].中华男科学杂志,2005,11(4)：290-295.
32.Sehaeffer AJ, Knauss JS, Landis JR, etal. Leukocyte and bacterial counts do not correlate with severity of symptoms in men with chronic prostatitis:the National Institutes of Health Chronic Prostatitis Cohort Study[J]. JUrol,2002,168:1048-1053.
33.唐孝达.慢性前列腺炎诊断与治疗进展[J].中国男科学杂志,2002,16(3)：193-196.
34.Shortliffe LM,Wehner N.The characterization of bacterial and nonbacterial proststitis by prostatic immunoglobulins.Medicine.1986,Nov,65(6):399-414.
35.Alexander RB,Ponniah,Hasday,etal.Elevate levels of proinflammatory cytokines in the semen of patients with chronic prostatitis.chronic pelvic pain syndrome[J].Urology.1998,52:744-749.
36.Batstone GR,Doble A,Gaston JS.Autoimmune T cell responses to seminal plasma in chronic pelvic pain inflammation(CPPS)[J].Clinical and Experimental mmunology,2002,128(2):302-303.
37.Ponniah S,Arah I,Alexander RB. PSA is a candidate self-antigen in autoimmune chronic prostatitis/ chronic pelvic pain syndrome[J]. Prostate,2000,44(1):49-50.
38.李树平,孟双艳等.慢性非细菌性前列腺炎患者血清和前列腺液4种细胞因子检测及意义[J]中华男科学杂志,2006,1：25-27.
39.李树平,孟双艳等.慢性前列腺炎患者血清细胞因子的水平变化及其意义[J]医学理论与实践,2006,3：266-267.
40.汪岩.赵忠文,王立波.前列腺液中细胞因子(IL-6、TNF-a)在慢性前列腺炎中的意义[J].自求恩医科大学学报,1999.25(4)：402.
41.孙一呜,刘丽.前列腺水丸对慢性细菌性前列腺炎大鼠血清IL-6和TNF-a水平的影响[J].中华男科学杂志,2006,5：470-472.
42.吴永吉,刘硕.慢性非细菌性前列腺炎症状与前列腺液中白介素一10水平的关系[J].医师进修杂志2005,28(10：37-38.
43.The efects of interleukin-10 and-8 in chronic prostatitis. Zhong Hua Nan Ke Xue。2004,10(7):486-490.
44.John H,Barghom A,Funke G,etal. Noninflammatory chronic pelvicpain syndrome:immunological study in blood,ejaculate and prostate tissue [J].Euro Urol,2001,39:72-78.
45.赵海军,韩保健,侯成玉.慢性非细菌性前列腺炎患者前列腺液中TNF-α、IL-10水平变化及意义[J].山东医药2007,47(21)：52-53.
46.Miller LJ, Fischer KA, Goralnick SJ. elaf. Interleukin.10 lcvels in seminal plasma:implications for chronic prostatitis-chronic pelvic pain syndrome[J]. JUrol,2002,167(2 Pt 1):753-756.
47.刘广省.土茯苓的药理研究进展[J].中药材,2001.24(8)：615-616.
48.芦绪芳.金银花的研究现状[J].时珍国医国药,2006,17(5)：843-844.
49李雪征,金光洙.萆薢的研究进展[J].中国野生植物资源,2002,21(5)：8-10.
50.邱静帆,殷颖.车前子的药学研究及其应用[J].浙江中西医结合杂志,2002,12(1)：55.
51.吕刚,李婷婷.石菖蒲的研究进展[J].时珍国医国药,2006,17(7)：1291-1292.
52.徐富一,金正子,申舜植.王不留行与硬叶女娄菜止痛、消炎效能的比较研究[J].河南中医学院学报,2006,21(2)：31-32.
53.马丽.穿山甲的药理及临床研究[J].中医药研究,2002.18(2)：46-47.
54.伍晓春,陆豫.虎杖的药理作用及临床应用研究进展[J].中医药信息,2005.22(2)：22-25.
55.赵兴梅,徐光忠,李建利等.川牛膝和怀牛膝的现代药理研究概况[J].华西药学杂志,2004,19(3)：205-207.
56.吕清文,丛雅勤.延胡索药理作用及临床应用浅议[J].内蒙古中医药,2005,01(01)：26.
57.史桂兰,胡志浩.甘草酸药理作用及临床应用研究进展[J].天津市医药科学杂志,2003,4(9)：11-12.
58..Loschen G. Ebeling L. Inhibition of the arachidonatc metabolism by an extract from rye pollen[J]. Drug Res.1991,41:162-167.
59.张亚强,卢建新,吴志奎等.丹蒲胶囊对慢性前列腺炎患者前列腺液IL-2、IL-6水平的影响[J].中国中西医结合杂志,2002.22(11)：828-829.
60.魏武然,张唯力,戴君勇.大鼠慢性非细菌性前列腺炎模型的建立.中国男科学杂志,2006,20(1)：222-224
61.徐叔云,卞如濂,陈修主编.药理实验方法学.第3版.北京：人民卫生出版社,2002：1557：911
62.Lang MD.Nickel C,Olson ME,et al.Rat model of experimentally induced abactedal pmstatitis[J]. Prostate 2000,45:201.
63.戴苏林,朱进,魏秀德,等.前列腺康栓对大鼠实验性非细菌性前列腺炎的抑制作用.中成药.1990,12(8)：27-28.
64.张亚强,刘猷枋.中药前列腺汤对实验性前列腺炎病理模型的影响.中国中西医结合杂志.1991,11(8)：480-482.
65.唐风珍,侯蕙茹,张景云,等.大鼠前列腺炎的制备方法.中国药理学通报.1998,14(2)：187-188.
66.Harritl MT Feldberg RS, Lau KM et aL. Exlxessing of proinflamatory genes during estrogen-induced inflammation of the rat prorate[J].Prorate 2000,44:19.
67.Michael J. Wlison,Mildced Woodson, Carol Wiehr,et al. Matrix metalloproteinases in the pathogencsis of estradiol-induced nonbactefial prostatitis in the lateral prostate lobe of the Wistar rat. Experimental and molecular Pathology [J].2004:77:7-17.
68. Kwon SM, Kim SI, Chun DC, et al. Development of rat prostatitis model by oral administration of isoflavone and its characteristics[J], Yonsei Med J,2001,42(4):395-404.
69.叶伟成,薛慈民,徐兆东等.免疫佐剂法制作慢性非细菌性前列腺炎小鼠模型的方法.中国男科学杂志2001.15(1)：29.
70. Keetch DW, Humphrey P, Ratliff TL. Development of a mouse model for nonbacterial prostatitis[J]. J Urol,1994,152(1):247-250.
71.Paulis G, Conti E, Voliani S, Bertozzi MA, et al. Evaluation of the cytokines in genital secretions of patients with chronic prostatitis[J]. Arch Ital Urol Androl,2003,75(4):179-186.
72.张庆云,莫曾南,刘硒碲.姜黄素对慢性非细菌性前列腺炎大鼠TNF-α、TL-6和IL-8表达的影响.中华男科学杂志,2010,16(1)：84-88.
73.王洪志,刘朝东,韦超IFN-γ、TNF-a和IL-10在慢性非细菌性前列腺炎大鼠中的表达及意义.重庆医科大学学报,2009,34(9)：1187-1190.
74.Miller LJ, Fischer KA, Goralnick SJ, et aL. Interleukin-10 levels in seminal plasma:implications for chronic prostatitis-chronic pelvic pain syndrome[J]. J Urol,2002,167(2):753-756.
75.赵海军,韩保健,侯成玉.慢性非细菌性前列腺炎患者前列腺液中TNF-α、IL-10水平变化及意义.山东医药,2007(21)：52-53.
[1]Robertson C, Boyle P, Nonis A,etal.International population based studu of urological conditions:the Urepik study. II.Comparative prostatitis data [J].Jurol,1999,161(4):32.
[2]Wenninger K, Heiman J R, Rothman I, etal. Sickness impact of chronic nonbacterial prostatitis and its correlates [J]. J Urol,1996,155(3):965-968.
[3]郭应禄,胡礼泉主编.男科学[M].北京：人民卫生出版社.2004：1485.
[4]郭霭春.黄帝内经素问校注[M].北京：人民军医出版社,1992年,第一版：571.
[5]李用粹.证治汇补[M].北京：科技卫生出版社,卷八：441.
[6]张景岳.景岳全书[M].北京：人民卫生出版社,1991年,第一版：640.
[7]伊广迁,李永占.明清十八家名医医案[M].北京：中国中医药出版社,1997：325-326.
[8]沈时誉.医衡[M].上海：上海书店,1985：67.
[9]程仲龄.医学心悟[M].北京：科学技术文献出版社,1996,第一版：159.
[10]谢映卢.得心集医案.珍本医学集成本[M].上海：世界书局,1936,112.
[11]叶天士.临证指南医案[M].北京：华夏出版社,1995：303-307.
[12]巢元方.诸病源候论[M].北京：人民卫生出版社,1982：128.
[13]林佩琴.类证治裁[M].北京：中国中医药出版社,1997年,第一版：197.
[14]徐春甫.古今医统大全[M].北京：人民卫生出版社,1991年：965.
[15]张锡纯.医学衷中参西录[M].石家庄：河北科技出版社,1985年：487.
[16]何梦瑶.医碥[M].上海：上海科学技术出版社,1982年,第一版：232.
[17]李兰群,周强.李曰庆论治慢性前列腺炎经验[J].山东中医杂志2002.12(12)：745-746.
[18]吴少刚,王琦治疗慢性前列腺炎的临床思维[J].山东中医杂志1994,13(10)：438-439.
[19]王天明.慢性前列腺炎的辨治体会[J].河南中医药学刊,2002,17(6)：64-65.
[20]张耀圣.谈前列腺炎复发的预防[J].光明中医,200217(4)：23-24.
[21]徐福松.辨证与辨病论治慢性前列腺炎[J].男科学报1999,5(1)：6-11.
[22]龚翰林、邹飞、李俊涛等.王久源教授辨治慢性前列腺炎临床经验[J].四川中医2005,23(3)：6-7.
[23]唐学会.郭军教授治疗慢性前列腺炎经验[J].河南中医2007,27(4)：15-16.
[24]石志超.从血瘀精道论治慢性前列腺炎[J].辽宁中医杂志,2001,28(11)：663-664.
[25]李承功.周智恒治疗前列腺疾病经验[J].江西中医药2002,33(2)：5.
[26]陆德铭主编.中医外科学[M].上海：上海科学技术出版社,1997：204-205.
[27]刘建邦,徐永明.辨证治疗慢性前列腺炎32例体会[J].新疆中医药,2002,20(1)：18.
[28]刘宁飞.辨证治疗慢性前列腺炎50例[J].湖南中医药导报,1997,3(4)：53.
[29]邹方伟,姜兆友,霍振国.辨证施治慢性前列腺炎102例[J].山东中医杂志,2003,22(5)：285.
[30]彭煜.泉宁方治疗非细菌性前列腺炎的临床观察[J].上海中医药杂志,2004,38(5)：35-36.
[31]王权胜,唐振宇.程氏萆藓分清饮治疗慢性前列腺炎40例效果观察[J].现代中西医结合杂志,2004,13(12)：1571-1572.
[32]叶志雄,谢长才.黄柏前列汤治疗慢性非细菌性前列腺炎的临床观察[J].湖北中医杂志,2005,27(6)：27-28.
[33]徐新建,周智恒.清热化瘀法治疗慢性非细菌性前列腺炎80例[J].中国中西医结合外科杂志.2005.11(3)：243-244.
[34]张朝德.疏肝通络法治疗慢性非细菌性前列腺炎疗效观察[J].吉林中医药,2004.24(9)：34-35.
[35]高洪寿,任平.从肝论治慢性非细菌性前列腺炎的疗效观察[J].中国男科学杂志,2002.16(4)：332-333.
[36]曾晔,赖海标,钟亮等.坐盆汤坐浴治疗慢性非细菌性前列腺炎的临床观察[J].中医外治杂志,2005.14(2)：16-17.
[37]孙天成,李君森,程美.六味地黄汤加味配合温热水坐浴治疗慢性无菌性前列腺炎[J].
中国临床医药研究杂志,2007(173：44.
[38]崔飞婵,苏斌,蔡东.中药灌肠加外敷治疗慢性非细菌性前列腺炎52例[J].新中医,2004.36(7)：67-68.
[39]罗建华,张西芝.“盆腔灵”直肠灌注治疗慢性非细菌性前列腺炎疗效观察[J].男科学报,1998.4(1)：62-63.
[40]陈其华,席建元.五圣消炎栓直肠给药治疗慢性前列腺炎90例[J].中国中医药信息杂志,2003.10(10)：53-54
[41]王尉,何恢绪,胡卫列等.前列腺内直接注射川参通治疗慢性非细菌性前列腺炎／慢性骨盆疼痛综合征[J].中华男科学杂志,2004.10(3)：182-187.
[42]刘紫庭,廖海球,杨菊云.川参通直接注入前列腺治疗慢性非细菌性前列腺炎／慢性盆腔疼痛综合征[J].中华男科学杂志,2005.11(9)：706-707.
[43]薄立宏.双黄连粉针剂膀胱内灌注治疗慢性前列腺炎18例[J].中医研究,1997.10(4)：34-35.
[44]吴玉清,李承功.中药口服合双黄连尿道灌注治疗慢性前列腺炎60例[J].中国民间疗法,2002.10(2)：24-25.
[45]于峰.自我按摩治疗慢性前列腺炎45例[J].按摩与导引,1997.3：42
[46]郑文华.清利化瘀汤加前列腺按摩治疗慢性非细菌性前列腺炎96例[J].广西中医学院学报,2006.9(2)：39-40.
[47]刘继能.自然特异功治疗慢性前列腺36例[J].气功与科学,1994.4：19.
[48]何天有,赵耀东,雒成林.针刺“三阴穴”治疗慢性前列腺炎临床观察[J].中国针灸,2004,24(10)：697—698.
[49]何馨.针灸治疗慢性前列腺炎36例临证观察[J].中医药学刊,2001.19(4)：387.
[50]葛继魁,葛书翰.深刺白环俞为主治疗慢性前列腺炎临床观察[J].中国针灸,2001,21(2)：73.
[51]孙自学,吴宏东.芒针治疗慢性非细菌性前列腺炎的临床研究[J].中医药学刊,2003.21(1)：77-78.
[52]张培永,王霄鹏.艾灸会阴与坐浴对照治疗非细菌性前列腺炎90例[J].中医外治杂志,1999.8(5)：13-14.
[53]陈昭典.前列腺炎综合征的历史演变与进展[J].中国男科学杂志,2003.17(6)：633-368.
[54]Drach GW, Fair WR, Meates EM. etal. Classification of benign diseases associated with prostatic Pain:prostatitis and prostatodynia[J]. Jurol 1978:120-266
[55]Krieger JN. Nyberg LJr, Nickel JC. NIH consensus definition and classification of prostatitis[J]. JAMA 1999.21:282(3):236-237。
[56]石理华,李瑛,李辉等.诊断为慢性非细菌性前列腺炎患者的前列腺液和前列腺组织中细菌基因的检测[J].武警医学报,2007.16(1)：26-27
[57]Nickel JC.Prostatitis:Evolving and management strategies[J].Urol clin North AM,1999,26(4):737-751
[58]Shoskes DA, Albakri Q, Thomas K, etal[J]. JUrol 2002; 168(1):331-335
[59]邓春华,梁宏,梅骅.前列腺内尿液返流在慢性前列腺炎发病中的作用[J].中华泌尿外科杂 志,1998,19(6)：288-298.
[60]陈琦,魏莎莉.慢性非细菌性前列腺炎／慢性盆腔疼痛综合征发病机制研究进展.国外医学泌尿系统分册,2005,25(5)：629
[61]谢辉,杨亦荣.前列腺痛患者情绪障碍的分析及治疗[J].临床泌尿外科杂志,2002,17(6)：288-289
[62]徐英民,高维忠,夏术阶等.普拉宗与泌尿灵联用治疗慢性非细菌性前列腺炎和前列腺痛的疗效观察[J].实用男科杂志,1996,2(1)：21-23.
[63]埃杜,莫曾南.特拉唑嗪治疗慢性前列腺炎／慢性盆腔疼痛综合征的系统评价[J].广西医学,2007,29(6)：789-792.
[64]ItoR, IshiM, YamaahitaS,etal. Antiprostatic hypertrophic action of Cernihon. pollen extract[J]. Pharmacometrics,1986.3:1-11.
[65]阎洪涛,廖勇,龚百生等.舍尼通治疗ⅢA型前列腺炎的临床疗效[J].中华男科学杂志,2004,10(12)：930-931.
[66]叶章群,蓝儒竹,王少刚等.舍尼通和抗生素联合用药治疗慢性非细菌性前列腺炎临床研究[J].中华男科学杂志,2006,12(9)：807-810.
[67]Persson BE,Ronquist G,EKb lomM. Ameliorative efect of allop urinol on nonbacterial prostatitis:a parallel double-blind controlled study[J].J Urol.l996.155(3):961-964.
[68]张培升,陈琴.抗菌药对慢性非细菌性前列腺炎的疗效观察[J].海峡药学,2001,13(3)：58-59.
[69]许明,张永康.抗菌药对慢性非细菌性前列腺炎的治疗作用[J].临床泌尿外科杂志,2000,15(5)：195-196.
[70]范华,甘肖余.慢性前列腺炎122例治疗体会[J].广西医学,2005,27(3)：416-417.
[71]蒋建光.经皮(会阴)穿刺行药物注射治疗慢性前列腺炎1640例[J].实用中西医结合临床,2007,7(4)：6-7.
[72]杨振玲, 卜宏民.骶管阻滞治疗慢性前列腺炎80例临床研究[J].河南外科学杂志,2005,11(5)：66.
[73]ZHOU Zhan-song,SONG Be,NIE Fa-chuan,et al.Treatment of intractable chronic pelvic pain syndrome by injecting a compound of Bupivacaine and Fentanyl into sacral spinal space[J].Journal of Medical Colleges of PLA.2006,21(4):258-260.
[74]黄慧,龙媛媛,柳青.尿道内药物灌注治疗非细菌性前列腺炎的观察[J].中国民康医学杂志.2004,16(11)：692。
[75]王爱民,杨斌,刘巧等.体外高频热疗治疗慢性前列腺炎临床疗效观察[J].临床皮肤科杂志,2004,33(12)：764
[76]林芝,黎承阳.射频热疗治疗慢性前列腺炎36例总结[J].右江医学杂志,1998,26(2)：77-78
[77]Nickel JC, Downey J, Feliciano AE, etal. Repetitive prostatic massagetherapy for chronic refractory pro statitis:the phililppin e expe rievee[J].TechUrol,1999,5(3):146-151
[78]周巧奇,朱湘生.前列腺按摩配合特拉唑嗪治疗慢性前列腺炎86例临床观察[J].中国性科学,2007,16(1)：22-23
[79]胡跃刚.慢性前列腺的药物综合治疗临床观察[J].中国医院药学杂志,2006,26(11)：1386-1387
[80]游秀英,宋万蝉.双囊三腔管前列腺药物灌注治疗非淋菌性前列腺炎100例临床分析[J].右江民族医学院学报,2006,6：996-997.
[81]倪颖,朱晓群,江汉群.综合心理、行为干预治疗慢性非细菌性前列腺炎效果观察[J].中国行为 医学科学,2002,11(6)：656-657.
[82]戴胜国,冷静,吕坚伟.中西医结合综合治疗慢性前列腺炎2284例分析[J].中国男科学杂志,2002,16(41)：302-304
[83]郑海滨,崔艳荣.综合疗法治疗慢性前列腺炎临床应用研究[J].中华男科学杂志.2004,10(9)：701-703
[84]吕建林.中西医结合治疗慢性前列腺炎150例疗效观察[J].新中医,2003,35(1)：42-43
[85]江先汉,郭燕东,张文海等.中西医结合治疗慢性前列腺炎的临床总结[J].现代医药卫生,2004,20(12)：1074-1075
[86]李云武,陈金雄,郑清水.中西医结合治疗慢性前列腺炎60例[J].实用中医药杂志,2002,18：30—31.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |