穴位埋线对变应性鼻炎大鼠鼻黏膜Th1/Th2，Treg/Th17的影响
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摘要
目的：
变应性鼻炎(AR)是耳鼻喉科常见病,多发病,是导致哮喘发生的主要因素之一。AR已经成为影响全球人群健康的多发性疾病。恩师张勤修教授认为该病主要为肺、脾、肾三脏功能失调,气阳虚衰为本,外感风邪为标,临床辨证分型以虚证为主,特别是肺脾气虚多见,治疗多采用补气固表,健脾益胃,升阳益气的方法。临床实践证明穴位埋线对AR具有良好的治疗效果。本课题通过动物实验验证穴位埋线治疗AR的疗效,并从病理形态、免疫学机制方面探讨其作用机制。
方法：
将健康SPF级SD大鼠70只随机分为空白组(Ⅰ)、AR模型组(Ⅱ)、AR西药组(Ⅲ)、AR穴位假埋线组(Ⅳ)、AR穴位埋线组(V)。采用经我们改进后的卵白蛋白注射及鼻黏膜刺激法进行AR大鼠造模。除空白组外各组予以不同治疗方法,治疗末日在卵白蛋白激发后,观察30分钟,了解大鼠AR症状情况并记录比较各组大鼠变应性鼻炎症状评分,之后立即处死动物,采集大鼠血液、鼻中隔黏膜。在电镜下观察大鼠鼻黏膜病理形态变化,采用ELISA方法检测大鼠血清IgE含量,采用免疫组化方法检测鼻黏膜组织IFN-γ、IL-4,TGF-β1、IL-17、P物质的表达情况。数据采用SPSS17.0软件进行统计分析。
结果：
穴位埋线能明显缓解AR大鼠变应性鼻炎症状(喷嚏、流涕、抓搔鼻部)。与模型组比较,穴位埋线能改善AR大鼠鼻黏膜病理改变,降低AR大鼠血清IgE含量,降低鼻黏膜中IL-4,TGF-β1、IL-17、P物质含量,升高IFN-γ含量。穴位假埋线能降低AR大鼠鼻黏膜中TGF-β1含量。
结论：
本研究通过卵白蛋白致敏法建立变应性鼻炎大鼠,对AR大鼠进行穴位埋线治疗,并设立西药组对(?)通过比较各组免疫指标我们发现：1、变应性鼻炎大鼠血清IgE,鼻黏膜P物质、IL-4、IL-17、TGF-βI表达明显
升高,鼻黏膜INF-γ表达降低,显示鼻黏膜Thl/Th2, Treg/Th17免疫平衡破坏是变应性鼻炎大鼠重要的病理改变。
2、穴位埋线可明显缓解SD大鼠变应性鼻炎相关症状。
3、穴位埋线能降低变应性鼻炎大鼠血清IgE含量；降低鼻黏膜中IL-4, IL-17,TGF-β1含量;升高鼻黏膜INF-γ含量,从而缓解变应性鼻炎炎症反应进程,调节变应性鼻炎大鼠鼻黏膜Thl/Th2,Treg/Th17免疫平衡。
4、穴位埋线与曲安奈德喷鼻剂相比,在对血清IgE,鼻黏膜中P物质、IL-4、 IL-17、TGF-β1.INF-γ的影响方面无明显差异。
Object:
Allergic rhinitis (AR) is a common frequently-occurring disease in otolaryngology, which is one of the main factors leading to asthma and has become a global public health impact. ZhangQinXiu professors consider that the pathogenesis of this disease is the lung, spleen, kidney disorders, Yang failure is basic, pathogeny is actuating factors for this disease. To therapy AR, Professor Zhang uses catgut implantation at acupoints to benefit lung and spleen and to rise Yang and exorcising pathogeny which has been approved effectively in clinic. This topic will approve the effect of catgut implantation at acupoints for AR through the animal experiment and discusses the mechanism based on pathological morphology and immunology.
Method:
Health SPF SD rats were randomly divided into blank group (Ⅰ), AR model group (Ⅱ), AR western medicine group (Ⅲ), AR false catgut implantation at acupoint (Ⅳ), AR catgut implantation at acupoint (Ⅴ). We made AR animal model according the method of improved ovalbumin injection and nasal mucosa stimulation. Every group received different therapies except blank group (Ⅰ). After last therapy day, we gave ovalbumin nasal mucosa stimulation,then we observed and record the AR symptoms to compare the symptom records. We killed SD rats and collected rats blood, septum mucosa to observe rat nasal mucosa pathological change under electron microscopy, to detect rat serum IgE content using ELISA method, to detect nasal mucosa tissue IFN-y, IL-4, TGF-β1, IL-17, SP expression using immunohistochemical method. We used the data SPSS17.0software for statistical analysis.
Outcome:
Catgut implantation at acupoints can obviously alleviate the symptoms allergic rhinitis (sneezing, runny nose, scratching) of AR rats. Compared with model group(Ⅱ), catgut implantation at acupoints can improve pathological changes of AR rat nasal mucosa,reduce IgE content in AR rat serum, reduce IL-4, TGF-β1, IL-17, SP content, increase IFN-γ content in the AR rat nasal mucosa. Catgut implantation at acupoints can reduce TGF-β1content AR rats in nasal mucosa.
Conelus i on:
This research established" allergic rhinitis rats model" by ovalbumin sensitization method. We operated Catgut implantation at acupoints in allergic rhinitis rats, and set up the control group (Triamcinolone acetonide nasal spray), then we found:
1、The serum IgE and nasal mucosa SP, IL-4, IL-17, TGF-β1increased and nasal mucosa INF-y lowered significantly in allergic rhinitis rats, which means Th1/Th2and Treg/Th17immune disbalance of nasal mucosa is the most important pathogenesis in AR rats.
2^'Catgut implantation at acupoints'can alleviate symptoms of AR rats.
3^'Catgut implantation at acupoints'can reduce IL-4, TGF-β1, IL-17, SP content, increase IFN-γ content in the AR rat nasal mucosa to regulate Th1/Th2and Treg/Th17immune balance in AR rat nasal mucosa.
4、Catgut implantation at acupoints' and Triamcinolone acetonide nasal spray' have similar effect at serum IgE and nasal mucosa substance P, IL-4, IL-17, TGF-β1、INF-γ in allergic rhinitis rats.
变应性鼻炎(AR)是耳鼻喉科常见病,多发病,是导致哮喘发生的主要因素之一。AR已经成为影响全球人群健康的多发性疾病。恩师张勤修教授认为该病主要为肺、脾、肾三脏功能失调,气阳虚衰为本,外感风邪为标,临床辨证分型以虚证为主,特别是肺脾气虚多见,治疗多采用补气固表,健脾益胃,升阳益气的方法。临床实践证明穴位埋线对AR具有良好的治疗效果。本课题通过动物实验验证穴位埋线治疗AR的疗效,并从病理形态、免疫学机制方面探讨其作用机制。
方法：
将健康SPF级SD大鼠70只随机分为空白组(Ⅰ)、AR模型组(Ⅱ)、AR西药组(Ⅲ)、AR穴位假埋线组(Ⅳ)、AR穴位埋线组(V)。采用经我们改进后的卵白蛋白注射及鼻黏膜刺激法进行AR大鼠造模。除空白组外各组予以不同治疗方法,治疗末日在卵白蛋白激发后,观察30分钟,了解大鼠AR症状情况并记录比较各组大鼠变应性鼻炎症状评分,之后立即处死动物,采集大鼠血液、鼻中隔黏膜。在电镜下观察大鼠鼻黏膜病理形态变化,采用ELISA方法检测大鼠血清IgE含量,采用免疫组化方法检测鼻黏膜组织IFN-γ、IL-4,TGF-β1、IL-17、P物质的表达情况。数据采用SPSS17.0软件进行统计分析。
结果：
穴位埋线能明显缓解AR大鼠变应性鼻炎症状(喷嚏、流涕、抓搔鼻部)。与模型组比较,穴位埋线能改善AR大鼠鼻黏膜病理改变,降低AR大鼠血清IgE含量,降低鼻黏膜中IL-4,TGF-β1、IL-17、P物质含量,升高IFN-γ含量。穴位假埋线能降低AR大鼠鼻黏膜中TGF-β1含量。
结论：
本研究通过卵白蛋白致敏法建立变应性鼻炎大鼠,对AR大鼠进行穴位埋线治疗,并设立西药组对(?)通过比较各组免疫指标我们发现：1、变应性鼻炎大鼠血清IgE,鼻黏膜P物质、IL-4、IL-17、TGF-βI表达明显
升高,鼻黏膜INF-γ表达降低,显示鼻黏膜Thl/Th2, Treg/Th17免疫平衡破坏是变应性鼻炎大鼠重要的病理改变。
2、穴位埋线可明显缓解SD大鼠变应性鼻炎相关症状。
3、穴位埋线能降低变应性鼻炎大鼠血清IgE含量；降低鼻黏膜中IL-4, IL-17,TGF-β1含量;升高鼻黏膜INF-γ含量,从而缓解变应性鼻炎炎症反应进程,调节变应性鼻炎大鼠鼻黏膜Thl/Th2,Treg/Th17免疫平衡。
4、穴位埋线与曲安奈德喷鼻剂相比,在对血清IgE,鼻黏膜中P物质、IL-4、 IL-17、TGF-β1.INF-γ的影响方面无明显差异。
Object:
Allergic rhinitis (AR) is a common frequently-occurring disease in otolaryngology, which is one of the main factors leading to asthma and has become a global public health impact. ZhangQinXiu professors consider that the pathogenesis of this disease is the lung, spleen, kidney disorders, Yang failure is basic, pathogeny is actuating factors for this disease. To therapy AR, Professor Zhang uses catgut implantation at acupoints to benefit lung and spleen and to rise Yang and exorcising pathogeny which has been approved effectively in clinic. This topic will approve the effect of catgut implantation at acupoints for AR through the animal experiment and discusses the mechanism based on pathological morphology and immunology.
Method:
Health SPF SD rats were randomly divided into blank group (Ⅰ), AR model group (Ⅱ), AR western medicine group (Ⅲ), AR false catgut implantation at acupoint (Ⅳ), AR catgut implantation at acupoint (Ⅴ). We made AR animal model according the method of improved ovalbumin injection and nasal mucosa stimulation. Every group received different therapies except blank group (Ⅰ). After last therapy day, we gave ovalbumin nasal mucosa stimulation,then we observed and record the AR symptoms to compare the symptom records. We killed SD rats and collected rats blood, septum mucosa to observe rat nasal mucosa pathological change under electron microscopy, to detect rat serum IgE content using ELISA method, to detect nasal mucosa tissue IFN-y, IL-4, TGF-β1, IL-17, SP expression using immunohistochemical method. We used the data SPSS17.0software for statistical analysis.
Outcome:
Catgut implantation at acupoints can obviously alleviate the symptoms allergic rhinitis (sneezing, runny nose, scratching) of AR rats. Compared with model group(Ⅱ), catgut implantation at acupoints can improve pathological changes of AR rat nasal mucosa,reduce IgE content in AR rat serum, reduce IL-4, TGF-β1, IL-17, SP content, increase IFN-γ content in the AR rat nasal mucosa. Catgut implantation at acupoints can reduce TGF-β1content AR rats in nasal mucosa.
Conelus i on:
This research established" allergic rhinitis rats model" by ovalbumin sensitization method. We operated Catgut implantation at acupoints in allergic rhinitis rats, and set up the control group (Triamcinolone acetonide nasal spray), then we found:
1、The serum IgE and nasal mucosa SP, IL-4, IL-17, TGF-β1increased and nasal mucosa INF-y lowered significantly in allergic rhinitis rats, which means Th1/Th2and Treg/Th17immune disbalance of nasal mucosa is the most important pathogenesis in AR rats.
2^'Catgut implantation at acupoints'can alleviate symptoms of AR rats.
3^'Catgut implantation at acupoints'can reduce IL-4, TGF-β1, IL-17, SP content, increase IFN-γ content in the AR rat nasal mucosa to regulate Th1/Th2and Treg/Th17immune balance in AR rat nasal mucosa.
4、Catgut implantation at acupoints' and Triamcinolone acetonide nasal spray' have similar effect at serum IgE and nasal mucosa substance P, IL-4, IL-17, TGF-β1、INF-γ in allergic rhinitis rats.
引文
1.孔维佳,等.耳鼻咽喉头颈外科学[M].第二版.北京：人民卫生出版社,2010：288.
2. Housquet J, Aahl R, Khaltaev N. Global alliance chronic reapiratory disease [J].Allergy,2007, 62:216-223.
3.王士贞.中医耳鼻咽喉科学[M].北京：中国中医药出版社,2006：124.
4.黄桂锋,卢健敏,熊大经,谢慧.培土生金法对脾气虚变应性鼻炎大鼠鼻黏膜组织STAT-6mRNA表达的影响.江西中医学院学报,2012,24(2)：67-69.
5.熊大经.主编.实用中医耳鼻咽喉口齿科学.上海：上海科学技术出版社.2001
6.刘继辉.曲安奈德治疗变应性鼻炎68例.中国药业,2012,21(21)：93-94.
7.赵义造,黄益灯,黄加云等.电针对变应性鼻炎大鼠模型IL-4及IFN-γ表达的影响[J].浙江中医杂志,2010,45(12)：916-918.
8.王静云.电针对过敏性鼻炎动物模型的干预作用及其免疫学机制研究.博士论文.2005年,辽宁中医药大学.
9.郑祥容,温木生.埋线疗法治百病[M].北京：人民军医出版社,2002.
10. Knipping S, Holzhausen HJ, Riederer A, et al.Allergic and idiopathic rhinitis:an ultrastructural study[J].Eur Arch Otorhinolaryngol,2009,266(8):1249-56.
11. Schaper C, Gustavus B, Koch B, et al.Effect of fluticasone on neuropeptides in nasal lavage in persistent allergic rhinitis[J]. Investig Allergol Clin Immunol.2010,20(3):214-21.
12. Weaver CT, et al.Interplay between the TH17 and TReg cell lineages:a (co-)evolutionary perspective[J].Nat Rev Immunol.2009 Dec;9(12):883-9. Review.
13. Eastaff-Leung N, et al. Foxp3+ regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J].Clin Immunol.2010 Jan;30(1):80-9.
14. RadK, et al.Th17 and allergy[J].Allergol Int.2008 Jun;57(2):121-34. Review.
15.Oboki f infectious on susceptibility to autoimmune and allergic diseases[J]. N Engl J Med,2002, 347(12):911-920.
16.Maes T, Tournoy KG, Joos GF.Gene Therapy for Allergic Airway Diseases[J]. Curr Allergy Asthma Rep.2011 Jan 18. [Epub ahead of print]
17. Chalastras T, et al. Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis:investigation using a liquid-based method[J]. Laryngol Otol.2008;122(7):700-6.
18. Bach JF. The effect oulovic S,et al. Grass pollen immunotherapy induces Foxp3-expressing CD4+ CD25+cells in the nasal mucosa[J]. Allergy Clin Immunol.2008,121(6):1467-72.
19.Kay AB. Allergy and allergic diseases:first of two partsE[J]. NEngl J Med,2001,344(1):303713.
20.Oboki K, et al.Thl7 and allergy[J].Allergol Int.2008 Jun;57(2):121-34. Review.
21.Ciprandi G, et al. Peripheral Th-17 cells in allergic rhinitis:New evidence[J].Int Immunopharmacol. 2010;10(2):226-9.
22.Weaver CT, et al.Interplay between the TH17 and TReg cell lineages:a coevolutionary perspective[J].Nat Rev Immunol.2009 Dec;9(12):883-9. Review.
23. Eastaff-Leung N, et al. Foxp3+regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J]. Clin Immunol.2010;30(1):80-9.
24.赵丽,马雄,邱德凯,Th17细胞/调节性T细胞平衡与自身免疫性肝病[J].胃肠病学.2009,4(6)：107-108.
25. Korn,Bettelli E, Oukka M, Kuehroo VK.IL-17 and Th17 Cells[J]. Arnu Rev Immunol.2009;27:485-517.
26.韩德民主编,副主编张罗,董震,王德云,林小平等.变应性鼻炎[M],人民卫生出版社,2007：149.
27.Redington AE, Roche WR, Holgate ST, et al. Co-localization of immunoreactive transforming growth factor-1 and decorin in bronchial biopsies from asthmatic and normal subjects[J]. Pathol.1998;186:410-415.
28.Blobe GC, Schiemann WP, Lodish HF. Role of transforming growth factor-in human disease[J]. N Engl J Med.2000;342:1350-1358.
29.崔瑾,欧桂珍,杨涛等.穴位埋线对脾虚大鼠免疫功能影响的实验研究[J].中国医药学报,1999,14(6)：14-16.
30.张光奇,向开维,杨孝芳,等.穴位埋线对实验性大鼠溃疡性结肠炎黏附分子CD44 CD54及白细胞介素2的影响[J].中国针灸,2002,22(11)：765.
31.韦玲菊,赵星星,杜艳.穴位埋线治疗变应性鼻炎的诊治概况[J].辽宁中医药大学学报.2009,11(12)：203-204.
32.华兴邦,李辞蓉,周浩良,等.大鼠穴位图谱的研制[J].实验动物与动物实验,1991,1：1-5.
33.黄桂锋.培土生金法治疗脾气虚变应性鼻炎大鼠的实验研究.博士论文,2010,成都中医药大学.
34.崔鲁佳,阮岩,李婵娟,秦丽娜.变应性鼻炎小鼠模型的建立与IL-4、IFN-γ水平检测.中国新技术新产品,2012,8：254.
35. Temelkovski J, Hogan SP, Shepherd DP, Foster PS, Kumar RK.An improved murine model of asthma: selective airway inflammation, epithelial lesions and increased methacholine responsiveness following chronic exposure to aerosolised allergen[J]. Thorax,1998:53:849-851
36.Takahashi N, Ar amaki Y, Tsuchiya S. Allergic rhinitis model with Brown Norwayr at and evaluation o f antialler gic dr ugs [J]. J Pharmacobio Dy n,1990,13(7):414.
37. Tanka K, Okam oto Y. A nasal aller gy model developed in the guinea pig by intr anasal applicat ion of 2, 4-to luene diiso cyanate [J]. I nt Ar chs Allergy Appl Immun,1988,85(4):392.
38.余洪猛,文三立,刘志刚,方勇,王华.豚草花粉变应原致过敏性鼻炎豚鼠模型的建立[J],上海实验动物科学,2001,21(4)：212-214.
39.余洪猛,文三立.蛔虫变应原致过敏性鼻炎豚鼠模型建立[J].上海实验动物科学,2000,20(4)：217-219.
40.黄山,许利平,周丽娟,等.鼻康胶囊治疗大鼠过敏性鼻炎的机理探讨[J].中成药,2006,28(4)：564-566.
41.张志明,孙树岩,季文樾.真菌性抗原致变应性鼻炎豚鼠模型建立仁[J].中国耳鼻咽喉头颈科,2005,12(5)：313-315.
42. Lange RW, etal [J]. ToxiCol SCi.1999;50(1):64-71.
43.张鑫悦,卢良,刘利兵,等.嶂螂致变应性鼻炎豚鼠模型的建立[J].第四军医大学学报,2009,30(3)：279-281.
44.陈子眠.抗过敏实验方法及过敏反应动物模型研究进展[J].云南中医学院学报.2000,23(3)：11-18.
45.胡艳菲,胡长庚,吴月琴,唐方.卵白蛋白诱发大鼠过敏性鼻炎模型不同方法的比较[J].吉林中医药.2011,31(10)：1023-1025.
46.章如新,江德胜,李兆基等.p物质能神经阻滞剂治疗变应性鼻炎的实验研究[J].中华耳鼻咽喉科杂志,1994,29(5)：282-285.
47.张德波,杨幼琪,冯诚,等.过敏反应与致敏剂量和致敏时间的关系研究[J].中国药品标准,2007(3)：20.
48.田道法.益气温阳活血法治疗变应性鼻炎.江苏中医药,2007,39(2)：3-4.
49.程坚,蔡外娇,张新民.中医药治疗变应性鼻炎辨证用药特点及分析.中华中医药杂志,2012,27(7)：1947-1950.
50.阮岩,陈蔚.王德鉴教授治疗鼻鼽经验介绍.新中医,2006,38(3)：12-13
51.严道南,吴继勇,马华安,周家璇,邢光前,郭小红,胡小燕,朱春晖.益气温阳方治疗变应性鼻炎肺脾虚寒证的临床研究.南京中医药大学学报,2012,28(6)：509-512.
52.杨占军,阮岩,廖榴业,王慧敏,张奕.温肾补阳法对变应性鼻炎患者转录因子T-bet/GATA-3表达的影响中医临床研究.2012,4(8)：6-7.
53.张世中,严道南.鼻敏温阳方治疗肾阳虚型变应性鼻炎20例疗效观察.云南中医中药杂志,2012,33(6)：29-30.
54.李淑良.从肾虚论治变应性鼻炎.江苏中医药,2007,39(2)：4
55.宋卫军,李湘,谢强.变应性鼻炎300例中医辨证分析.中国社区医师：医学专业,2012,14(8)：219.
56.陶敏慧,魏效峰,谢强,刘新红.谢强运用“温督祛霾”针灸疗法治疗变应性鼻炎的临床经验.中国民族民间医药杂志,2011,20(12)：138
57.王秀玲,胡金秀,谢强,黄冰林.应用谢强“升阳祛霾”法治疗变应性鼻炎60例临床观察.实用中西医结合临床.2009,9(6)：48-49.
58.苏妆.背俞穴主治规律分析[J].医学综述,2008,14(13)：2076-2078.
59.郑祥容,温木生.埋线疗法治百病[M].北京：人民军医出版社,2002.
60.韦玲菊,赵星星,杜艳.穴位埋线治疗变应性鼻炎的诊治概况[J].辽宁中医药大学学报,2009：11(12)：203-204.
61.华兴邦,李辞蓉,周浩良,等.大鼠穴位图谱的研制[J].实验动物与动物实验,1991,1：1-5.
62.Gawlik R, DuBuske L. Mediator release of neuroprptides after nasal provocation in perennial allergic rhinitis patients. Rhinology.2010,48(2):206-210.
63.Cima K, Voqelsinqer H, Kahler CM. Sensory neuropeptides are potent chemoattractants for human basophils in vitro.Requl Pept.2010,160 (1-3):42-48.
64. Pfaar O, Raap U, Holz M, et al. Pathophysiology of itching and sneezing in allergic rhinitis. Swiss Med Wkly.2009 24;139(3-4):35-40.
65. Sarin S, Undem B, Sanico A, et al. The role of the nervous system in rhinitis. J Allergy Clin Immunol. 2006,118(5):999-1016.
66. Heppt W, Dinh Q T, Cryer A. Phenotypic alteration of neuropeptide containing nerve fibres in seasonal intermittent allergic rhinitis. Clin Exp Allergy,2004,34:1105-1110.
67.杨旭东,孙光明,许学谷,等.组胺H3受体激动剂IMETIT对大鼠变应性鼻炎作用的初步探讨.临床耳鼻咽喉头颈外科杂志.2010,2(12)：559-563
68.陈金湘,杨瑞喜,黄之春.变应性鼻炎神经肽与肥大细胞关系的试验研究.临床耳鼻咽喉头颈外科杂志.2007,21(20)：947-949.
69.章如新,刘国钧,温武,等.P物质在变应性鼻炎中对一氧化氮作用的研究[J].第二军医大学学报,2005,26(6)：654-656.
70. Asadi S, Alysandratos KD, Angelidou A,et al. Substance P (SP) Induces Expression of Functional Corticotropin-Releasing Hormone Receptor-1 (CRHR-1) in Human Mast Cells. J Invest Dermatol. 2012,132 (2):324-329.
71.韩德民.变应性鼻炎[M].人民卫生出版社,第一版,2007：80.
72. Sergio Romagnani. The role of lymphocytes in allergic disease [J].Journal of Allergy and Clinical Immunology,2000,105(3):399.
73.席斌,田道法.变应性鼻炎肺气虚证与血清IL-4和IFN-γ相关性的实验研究.中国中西医结合耳鼻咽喉科杂志,2007,15(3)：166-170.
74. Bach JF.The effect of infectious on susceptibility to autoimmune and allergic diseases[J].N Engl J Med,2002,347(12):911-920.
75.Knipping S, Holzhausen HJ, Riederer A, et al.Allergic and idiopathic rhinitis:an ultrastructural study[J].Eur Arch Otorhinolaryngol,2009,266(8):1249-56.
76.Schaper C, Gustavus B, Koch B, et al.Effect of fluticasone on neuropeptides in nasal lavage in persistent allergic rhinitis[J].Investig Allergol Clin Immunol.2010,20(3):214-21.
77. Maes T, Tournoy KG, Joos GF.Gene Therapy for Allergic Airway Diseases[J]. Curr Allergy Asthma Rep.2011 Jan 18. [Epub ahead of print]
78.Chalastras T, et al. Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis:investigation using a liquid-based method[J]. Laryngol Otol.2008,122(7):700-6.
79. Durham SR. Gould HJ, Thienes CP, et al. Local control of e-gene expression in B cells of the mucosa in hayfever patients following allergen challenge[J]. Allergy C lin Immunol,1996,97(2):297-302.
80.许丽娟.变态反应性鼻炎的某些研究进展[J].中国中西医结合杂志,2002,10(1)：50.
81.黄少鹤,张棍生.变应性鼻炎患者白细胞介素4、γ干扰素水平与1gE的相关性研究[J].福建医药杂志,2004,26(2)：14-16.
82.李钦,杜英华,陈彦林等.鼻腔吸入IFN-γ治疗变应性鼻炎的实验研究.山东大学耳鼻喉眼学报,2007,21(1)：51-53.
83.刘国梁,施举红,林耀光,等T-bet基因修饰树突细胞对哮喘模型小鼠气道炎症的阻抑和逆转作用[J].中华医学杂志,2009,89(8)：519-523.
84.赵义造,黄益灯,黄加云等.电针对变应性鼻炎大鼠模型IL-4及IFN-γ表达的影响[J],浙江中医杂志,2010，45(12)：916-918.
85.Oboki K, et al.Th17 and allergy[J].Allergol Int.2008 Jun;57(2):121-34. Review.
86.Ciprandi G, et al. Peripheral Th-17 cells in allergic rhinitis:New evidence[J].Int Immunopharmacol. 2010;10(2):226-9.
87.Weaver CT, et al.Interplay between the TH17 and TReg cell lineages:a coevolutionary perspective[J].Nat Rev Immunol.2009 Dec;9(12):883-9. Review.
88. Eastaff-Leung N, et al. Foxp3+regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J].J Clin Immunol.2010;30(1):80-9.
89. ParkH,Liz, Yangxo.etal. A distinct lineage of CD4+T cells regulates tissue inflammation by Producing interleukinl7[J].Natlmmunol,2005,6(11):1133-1141.
90. Sallusto F, Lanzaveeehia A. Heterogeneity of CD4+memory T cells:functional modules for tailored immunity[J]. Eur J Immunol.2009;39:2076-2082.
91. StockingerB.VeldheenM. Dirgerentiation and function of Th 17 T cells. Curt OPin.ImmunoI, 2007,19:281-286.
92. Dong C.TH17 cells indevelopment:an updated view of their molecular identity and genetic programming[J]. Nat Rev Immunol.2008;8:337-348.
93. Mangan PR,Harrington LE,Quinn DB,et al.Transforming growth factor-beta induces development of the Th17 lineage[J].Nature,2006,441(7090):231-234.
94. Korn,Bettelli E.Oukka M, Kuehroo VK.IL-17 and Th17 Cells[J]. Arnu Rev Immunol.2009;27:485-517.
95. Nakae S,Nambu A,Sudo K,et al. Suppression of immune induction Of collagen-induced arthritis in IL-17-deficient mice[J].Immunol,2003,171 (11):6173-6177.
96. Koenders MI. Lubberts E, Oppers-walgreenb, et al. Blocking of interleukin-17 during reactivation o experimentalanhritis prevents joint inflammation and bone erosion by decreasing RANKL and interleukin-1[J].Am J Pathol,2005,167(1):141-149.
97.赵丽,马雄,邱德凯.Th17细胞/调节性T细胞平衡与自身免疫性肝病[J].胃肠病学,2009,4(6)：107-108.
98. Harrington LE,HattonRD,ManganPR,etal.Interzeukin17-produeingeD4+Effector T cells develop Via a lineage distinct from the T helper type 1 and 2 lineages[J]. Nature Immunol,2005,6(11):1123-1132.
99. Sakaguchi s, Sakaguchi N, Asano M, et al. Immunologic self-toleranee maintained by activated T cells expressing IL-2 receptoralpha-chains(CD25). Breakdown of a single mechanism of selftolerance causes various autoimmune diseases[J]. J Immunol,1995,155(3):1151-1164.
100. Nistala K,Moncrieffe H, Newton KR,Varsani H,,Hunter P, Wedderburn LR. Interleukin-17-Producing T cells are enriched in the joints of children with arthritis, but have a Reciprocal relationship to regulatory T cell numbers [J].Arthritis Rheum.2008:58:875-887.
101. Moses H L,Branum E L,Proper J A,et al.Transforming growth factor production by chemically transformed cells[J]. Cancer Res,1981,41(7):2842-2848.
102.陈芳,李晓艳T-bet、TGF-β在过敏性鼻炎中的研究进展[J].山东大学耳鼻喉眼学报,2012,26(2)：83-85.
103. Karagiannidis C,Akdis M,Holopainen P,et al.Glucocorticoids up-regulate FOXP3 expression and regulatory T cells in asthma[J] J Allergy Clin Immunol,2004,114(6):1425-1433.
104.韩德民主编,副主编张罗,董震,王德云,林小平等.变应性鼻炎,人民卫生出版社2007：149.
105. Chen C H,Seguin-Devaux C,Burke N A,et al.Transforming growth factor beta blocks Tec kinase phosphorylation, Ca2+ influx, and NFATc translocation causing inhibition of T cell differentiation[J]. Exp Med,2003,197(12):1689-1699.
106. Scherf W,Burdach S,Hansen G. Reduced expression of transforming growth factor beta 1 exacerbates pathology in an experimental asthma model[J]. Eur J Immunol,2005,35(1):198-206.
107. Redington AE, Roche WR, Holgate ST, et al. Co-localization of immunoreactive transforming growth factor-1 and decorin in bronchial biopsies from asthmatic and normal subjects[J].J Pathol. 1998,186:410-415.
108. Blobe GC, Schiemann WP, Lodish HF. Role of transforming growth factor-in human disease[J]. N Engl J Med.2000;342:1350-1358.
109. Salib R J,Howarth P H.Transforming growth factor-beta in allergic inflammatory disease of the upper airways:friend or foe?[J].Clin Exp Allergy,2009,39(8):1128-1135.
110.Olsson N,Piek E,ten Dijke P,et al. Human mast cell migration in response to members of the transforming growth factor-beta family[J].J Leukoc Biol,2000,67(3):350-356.
111. Ouyang Y,Miyata M,Hatsushika K,et al. TGF-beta signaling may play a role in the development of goblet cell hyperplasia in a mouse model of allergic rhinitis[J]. Allergol Int,2010,59(3):313-319.
112. Barth RF. Boron neutron capture therapy at the crossroads:challengesand opportunities[J].Appl Radiat Isot,2009,67(7-8):S3-6.
113. Taylor A, Verhagen J, Akdis C A, et al. T regulatory cells in allergy and health:a question of allergen specificityand balance[J]. Int Arch Allergy Immunol,2004,135(1):73-82.
114. V ignola AM, Chan ez P, Ch iappara G, et al.Transforming growth factor-beta express ion in m ucosal biopsies in asthma and chronic bronchitis. J Am JRespir C ritC areMed,1997;156:591-9.
115. Pudd icombe SM, Polosa R, R ichterA, et al. Involvement of the epidermal growth factor receptor in epithelial repair in asthma. J FASEB J,2000; 14:1362-74.
116. Kenyon NJ, Ward RW, Grew GM,et al.TGF-1 causes airway fibrosis and increased collagen Ⅰ and Ⅲ mRNA in mice. Thorax.2003;58:772-777.)
117.刘争,陆翔,王恒.转化生长因子β1白细胞介素-6及11和17在变应性鼻炎患者鼻黏膜中的表达.临床耳鼻咽喉科杂志,2006,20(14)：625-627.
118.雷霏、许晶,徐洪君,等.实验性变应性鼻炎豚鼠鼻黏膜中TGF-β1表达及意义[J].中国老年学杂志2008,28(6)：533-534.
119. Zhang H, Phan S. Inhibition of myofibroblast apoptosis by transforming growth factor-1. Am J Respir Cell Mol Bio.1999;21:658-65.
120.于睿莉,朱冬冬,董震.中华耳鼻咽喉头颈外科杂志.2006；41(10)：773-776.
121. Lee, SS,Won, TB.Kim, JW,et al. Effects of Dexamethasone on the Expression of Transforming Growth Factor-[beta] in the Mouse Model of Allergic Rhinitis [J]. Laryngoscope.2007;117(8): 1323-1328.
122.杨长树.曲安奈德鼻喷雾剂治疗儿童过敏性鼻炎的临床疗效观察.中国实用医学,2011,6(25)：129-130.
123.介华.曲安奈德鼻喷雾剂在过敏性鼻炎中的临床应用.中国实用医学,2012,7(21)：183-184.
124.崔雪峰,田建华.曲安奈德鼻喷雾剂治疗变应性鼻炎的临床观察.承德医学院学报,2010,27(1)：18-19.
125.谷小鸣,方薇,方润琪.曲安奈德鼻喷雾剂和伯克纳喷鼻剂治疗过敏性鼻炎的平行对照试验.中华临 床医学杂志,2004,5(7)：45-46.
1.王士贞.中医耳鼻咽喉科学[M].北京：国中医药出版社,2006：124.
2.刘兆琳,侯慎英,赵立新.过敏煎治疗变应性鼻炎90例[J].河北中医,2005,27(3)：227.
3.朱正民,耿以安,陈虹.小青龙汤加减治疗过敏性鼻炎[J].湖北中医杂志,2004,26(5)： 38-39.
4.贾德蓉.浅谈“从肝入手、分期论治变应性鼻炎”[J].中医耳鼻喉科学研究杂志,2010,9(1)：32-33.
5.郭强中,李云英.柴胡桂枝汤治疗过敏性鼻炎经验[J].中医药报,2008,7(4)：53-54.
6.彭建中.清晨发病从肝论治验案举隅[J].北京中医药大学学报,2002,25(6)：66.
7.张士国.复方辛黄滴鼻液治疗鼻炎1637例疗效观察[J].山东中医杂志,2001,20(5)：
34-38
8.郑昌雄.中医耳鼻咽喉科学[M].上海：上海科技教育出版社,第一版.1995.
9.彭向军.中药加塞鼻剂治疗过敏性鼻炎40例体会[J].甘肃中医,2002,(4)：25.
10.赵义造,黄益灯,黄加云等.电针对变应性鼻炎大鼠模型IL-4及IFN-γ表达的影响[J].浙江中医杂志,2010,45(12)：916-918.
11.王静云.电针对过敏性鼻炎动物模型的干预作用及其免疫学机制研究.博士论文.2005年,辽宁中医药大学.
12.何天有,李惠琴,赵耀东,等.透刺为主治疗过敏性鼻炎60例[J].中国针灸,2006,26(2)：110.
13石学敏.针灸学[M].北京.中国中医药出版社.2002.
14.杨冠军,刘艳丽,许卫国.隔姜灸背俞穴治疗过敏性鼻炎60例[J].中国针灸,2001,21(3)：148.
15.胡志平,李小军,黄克伟.隔附子饼灸治疗过敏性鼻炎82例[J].上海针灸杂志,2005,24(9)：39.
16.周琦,曹明根,张雷.赵氏灸法治疗常年性过敏性鼻炎70例[J].上海中医药杂志,2001,35(9)：27.
17.寻满湘.穴位注射治疗变应性鼻炎[J].中国中西医结合耳鼻咽喉科杂志,2002(4)：180-181.
18.刘明清,谢小明,刘俐,等.穴位注射治疗变应性鼻炎的疗效观察[J].上海针灸杂志,2006(8)：10-11.
19.杨杰.卡介菌多糖核酸穴位注射辅助治疗常年性变应性鼻炎的疗效观察[J].中医药临床杂志,2009(6)：539-540.
20.郑祥容,温木生.埋线疗法治百病[M].北京：人民军医出版社,2002.
21.韦玲菊,赵星星,杜艳.穴位埋线治疗变应性鼻炎的诊治概况[J].辽宁中医药大学学报,2009：11(12)：203-204.
22.田雪秋,席中原,牟开金.穴位埋线治疗过敏性鼻炎50例[J].中医外治杂志,2011,20(6)：23.
23.邹昆.穴位埋线配合艾灸治疗变应性鼻炎疗效观察.中医药临床杂志,2011,23(7)：599-600.
24.秦尔奇,苑家敏.穴位敷贴治疗变应性鼻炎研究进展[J].中医研究,2012,25(2)：74-76.
25.陈传江,吴强,林栋.经络假说与穴位贴敷作用机理探讨[J].福建中医学院学报,2006,16(1)：26—28.
26.郎笑梅.夏季穴位敷贴治疗过敏性鼻炎30例[J].中医药临床杂志,2007,19(1)：31-32.
27.罗开涛,边晓东.穴位贴敷治疗过敏性鼻炎60例[J].浙江中医杂志,2011,46(1)：23-24.
28.刘光荣.内服中药苍耳辛夷散外加穴位按摩治疗过敏性鼻炎39例[J].按摩与导引,2004,20(04)：26.
29.赵国瑞,赵长云,林禄.中药滴鼻配合穴位按摩治疗过敏性鼻炎106例疗效观察[J].中国中医药科技,2002,9(2)：119.
30.李漫.耳穴贴压法治疗变应性鼻炎的疗效观察[J].医学综述2007,13(23)：1888-1889.
31.唐泳华.王不留行耳穴贴敷治疗变应性鼻炎[J].中国民间疗法,2001,9(02)：25.
32.齐伟,王朝辉.耳穴贴压治疗过敏性鼻炎50例临床观察[J].长春中医药大学学报,2008,24(6)：721.
33.朱向阳,邓耀成.耳穴药物注射法治疗过敏性鼻炎[J].针灸临床杂志,2000,16(06)：46-47.
34.吴震西,张锦秀.发泡膏贴治过敏性鼻炎64例[J].中医杂志,1988,09：44.
35.汤之明.斑鳌浸出液发泡治疗常年性变态反应性鼻炎的疗效及其血清总IgE的形响[J].中国中西医结合杂志,1995,15(6)：334.
36.杜昌华.耳背刺血治疗过敏性鼻炎60例[J],实用中医药杂志,1998,14(7)：37.
37.姚靳里拔罐疗法治疗常年性变态反应性鼻炎68例小结[J]中国中西医结合耳鼻咽喉科杂志,2008(5)：211.
38.孔维佳,周梁,许庚等.耳鼻咽喉头颈外科学[M].北京：人民卫生出版社,2005：75.
39.孔维佳,等.耳鼻咽喉头颈外科学[M].第二版.北京：人民卫生出版社,2010：288.
40. Leung R, Ho P. Asthma, allergy, and atopy in the three southeast Asian population[J]. Thorax.1994;49:1205-1210.
41. Celedon JC, Palmer LJ. Weiss ST, et al. Asthma, rhinitis, and skin test reactivity to aerollergens in families of asthmatic subjects in Anqing, china[J]. Am J Respir Crit Care Med.2001:163:1108-1112.
42.中华医学会耳鼻咽喉科学分会,中华耳鼻咽喉科杂志编辑委员会.变应性鼻炎诊断及疗效评定标准[J].中华耳鼻咽喉科杂志.1998：33：134-135.
43. Housquet J, Aahl R, Khaltaev N. Global alliance chronic reapiratory disease [J]. Allergy,2007,62:216-223.
44. BousquetJ, vanCauwenbergeP, KhaltaevN. Allergic rhinitis and its impact on asthma[J].J Allergy Clinlmmunol,2001,108(5):147-334.
45. Bousquet J, Khaltaev N, Cruz AA, et al. Allergic Rhinitis and its Impact on Asthma (ARIA) 2008 update(in collaboration with the World Health Organization, GA2LEN and AllerGen) [J]. Allergy,2008,63:S8-160.
46. Brozek JL, Bousquet J, Baena-Cagnani CE, et al. Allergic Rhinitis and its Impact on Asthma (ARIA)guidelines:2010 Revision [J]. J Allergy Clin Immunol, 2010,126:466-476.
47.孔维佳,周梁,许庚等.耳鼻咽喉头颈外科学[M].北京：人民卫生出版社,2005：75-76
48.中华耳鼻咽喉头颈外科杂志编委会鼻科组。变应性鼻炎诊断和治疗指南解读[J].中华耳鼻咽喉头颈外科杂志,2009,44(12)：972-976.
49.韩德民主编.变应性鼻炎[M].人民卫生出版社,2007,109-117。
50.张罗,周兵,韩德民,等.变应性鼻炎研究进展(一)：发病机制[J].耳鼻咽喉：头颈外科,2003,10(5)：316-320.
51. Strachan DP. Hayfever, hygiene and house hold size[J].BVJ,1989; 299:1259-1260.
52.Strachan DP. Familysize, infection and atopy:the first decade of the "hygiene hypothesis" [J]. Thorax,2000;55(Suppl1)2-10.
53. Bachert C, Gevaert P, van Cauwenberge P. Staphylococcus aureus enterotoxin:a key in airway disease? [J]. Allergy,2002,57(6):480-487.
54.孔维佳,周梁,许庚等.耳鼻咽喉头颈外科学[M].北京：人民卫生出版社,2005：77
55. Shiomori T, Yoshida S, Miyamoto H, Makishima K. Relationship of nasal carriage of Staphylococcus aureus to pathogenesis of perennial allergic rhinitis [J]. J Allergy Clin Immunol,2000,105(3):449-454.
56. Rossi RE, Monasterolo G. Prevalence of serum IgE antibodies to the Staphylococcus aureus enterotoxins(SEA, SEB, SEC, SED, TSST-1) in patients with persistent allergic rhinitis [J]. Int Arch Allergy Immunol,2004,133 (3):261-266.
57. Holt PG, Macaubas C, Stumbles PA, et al.The role of allergy in the development of asthma. Nature.1999;402:12-17.
58.孔维佳,周梁,许庚等.耳鼻咽喉头颈外科学[M].北京：人民卫生出版社,2005：77
59. Vamey VA, Jacobson MR, Sudderick RM, et al. Immuunohistilogy of thenasal mucosa following allergen-induced rhinitis. Identification of activated T lymphocytes, eosinophils, and neutrohpils. Am Rev Respire Dis.1992, 146:170-176.
60.Robinson DS, Hamid G, Ying S, et al. Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma[J]. NEng J Med.1992,326:298-304
61. Knipping S, Holzhausen HJ, Riederer A, et al. Allergic and idiopathic rhinitis: an ultrastructural study[J].Eur Arch Otorhinolaryngol, 2009,266(8):1249-56.
62.Schaper C, Gustavus B, Koch B, et al. Effect of fluticasone on neuropeptides in nasal lavage in persistent allergic rhinitis[J].J Investig Allergol Clin Immunol.2010,20(3):214-21.
63.Weaver CT, et al. Interplay between the TH17 and TReg cell lineages:a (co-)evolutionary perspective[J]. Nat Rev Immunol.2009;9(12):883-9.
64. Eastaff-Leung N, et al. Foxp3+ regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J].J Clin Immunol.2010 Jan; 30(1):80-9.
65.Maes T, Tournoy KG, Joos GF.Gene Therapy for Allergic Airway Diseases[J]. Curr Allergy Asthma Rep.2011 Jan 18. [Epub ahead of print]
66. Chalastras T, et al. Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis:investigation using a liquid-based method[J].J Laryngol Otol. 2008;122(7):700-6.
67. Bach JF. The effect of infectious on susceptibility to autoimmune and allergic diseases[J]. N Engl J Med,2002,347(12):911-920.
68.Kay AB. Allergy and allergic diseases:first of two parts[J]. NEngl J Med,2001,344(1):303713.
69.Oboki K, et al.Th17 and allergy[J]. Allergol Int.2008 Jun;57(2):121-34. Review.
70.Ciprandi G, et al. Peripheral Th-17 cells in allergic rhinitis:New evidence[J]. Int Immunopharmacol.2010;10(2):226-9.
71. Weaver CT, et al. Interplay between the TH17 and TReg cell lineages:a coevolutionary perspective[J]. Nat Rev Immunol.2009;9(12):883-9. Review.
72. Eastaff-Leung N, et al. Foxp3+regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J].J Clin Immunol. 2010;30(1):80-9.
2. Housquet J, Aahl R, Khaltaev N. Global alliance chronic reapiratory disease [J].Allergy,2007, 62:216-223.
3.王士贞.中医耳鼻咽喉科学[M].北京：中国中医药出版社,2006：124.
4.黄桂锋,卢健敏,熊大经,谢慧.培土生金法对脾气虚变应性鼻炎大鼠鼻黏膜组织STAT-6mRNA表达的影响.江西中医学院学报,2012,24(2)：67-69.
5.熊大经.主编.实用中医耳鼻咽喉口齿科学.上海：上海科学技术出版社.2001
6.刘继辉.曲安奈德治疗变应性鼻炎68例.中国药业,2012,21(21)：93-94.
7.赵义造,黄益灯,黄加云等.电针对变应性鼻炎大鼠模型IL-4及IFN-γ表达的影响[J].浙江中医杂志,2010,45(12)：916-918.
8.王静云.电针对过敏性鼻炎动物模型的干预作用及其免疫学机制研究.博士论文.2005年,辽宁中医药大学.
9.郑祥容,温木生.埋线疗法治百病[M].北京：人民军医出版社,2002.
10. Knipping S, Holzhausen HJ, Riederer A, et al.Allergic and idiopathic rhinitis:an ultrastructural study[J].Eur Arch Otorhinolaryngol,2009,266(8):1249-56.
11. Schaper C, Gustavus B, Koch B, et al.Effect of fluticasone on neuropeptides in nasal lavage in persistent allergic rhinitis[J]. Investig Allergol Clin Immunol.2010,20(3):214-21.
12. Weaver CT, et al.Interplay between the TH17 and TReg cell lineages:a (co-)evolutionary perspective[J].Nat Rev Immunol.2009 Dec;9(12):883-9. Review.
13. Eastaff-Leung N, et al. Foxp3+ regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J].Clin Immunol.2010 Jan;30(1):80-9.
14. RadK, et al.Th17 and allergy[J].Allergol Int.2008 Jun;57(2):121-34. Review.
15.Oboki f infectious on susceptibility to autoimmune and allergic diseases[J]. N Engl J Med,2002, 347(12):911-920.
16.Maes T, Tournoy KG, Joos GF.Gene Therapy for Allergic Airway Diseases[J]. Curr Allergy Asthma Rep.2011 Jan 18. [Epub ahead of print]
17. Chalastras T, et al. Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis:investigation using a liquid-based method[J]. Laryngol Otol.2008;122(7):700-6.
18. Bach JF. The effect oulovic S,et al. Grass pollen immunotherapy induces Foxp3-expressing CD4+ CD25+cells in the nasal mucosa[J]. Allergy Clin Immunol.2008,121(6):1467-72.
19.Kay AB. Allergy and allergic diseases:first of two partsE[J]. NEngl J Med,2001,344(1):303713.
20.Oboki K, et al.Thl7 and allergy[J].Allergol Int.2008 Jun;57(2):121-34. Review.
21.Ciprandi G, et al. Peripheral Th-17 cells in allergic rhinitis:New evidence[J].Int Immunopharmacol. 2010;10(2):226-9.
22.Weaver CT, et al.Interplay between the TH17 and TReg cell lineages:a coevolutionary perspective[J].Nat Rev Immunol.2009 Dec;9(12):883-9. Review.
23. Eastaff-Leung N, et al. Foxp3+regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J]. Clin Immunol.2010;30(1):80-9.
24.赵丽,马雄,邱德凯,Th17细胞/调节性T细胞平衡与自身免疫性肝病[J].胃肠病学.2009,4(6)：107-108.
25. Korn,Bettelli E, Oukka M, Kuehroo VK.IL-17 and Th17 Cells[J]. Arnu Rev Immunol.2009;27:485-517.
26.韩德民主编,副主编张罗,董震,王德云,林小平等.变应性鼻炎[M],人民卫生出版社,2007：149.
27.Redington AE, Roche WR, Holgate ST, et al. Co-localization of immunoreactive transforming growth factor-1 and decorin in bronchial biopsies from asthmatic and normal subjects[J]. Pathol.1998;186:410-415.
28.Blobe GC, Schiemann WP, Lodish HF. Role of transforming growth factor-in human disease[J]. N Engl J Med.2000;342:1350-1358.
29.崔瑾,欧桂珍,杨涛等.穴位埋线对脾虚大鼠免疫功能影响的实验研究[J].中国医药学报,1999,14(6)：14-16.
30.张光奇,向开维,杨孝芳,等.穴位埋线对实验性大鼠溃疡性结肠炎黏附分子CD44 CD54及白细胞介素2的影响[J].中国针灸,2002,22(11)：765.
31.韦玲菊,赵星星,杜艳.穴位埋线治疗变应性鼻炎的诊治概况[J].辽宁中医药大学学报.2009,11(12)：203-204.
32.华兴邦,李辞蓉,周浩良,等.大鼠穴位图谱的研制[J].实验动物与动物实验,1991,1：1-5.
33.黄桂锋.培土生金法治疗脾气虚变应性鼻炎大鼠的实验研究.博士论文,2010,成都中医药大学.
34.崔鲁佳,阮岩,李婵娟,秦丽娜.变应性鼻炎小鼠模型的建立与IL-4、IFN-γ水平检测.中国新技术新产品,2012,8：254.
35. Temelkovski J, Hogan SP, Shepherd DP, Foster PS, Kumar RK.An improved murine model of asthma: selective airway inflammation, epithelial lesions and increased methacholine responsiveness following chronic exposure to aerosolised allergen[J]. Thorax,1998:53:849-851
36.Takahashi N, Ar amaki Y, Tsuchiya S. Allergic rhinitis model with Brown Norwayr at and evaluation o f antialler gic dr ugs [J]. J Pharmacobio Dy n,1990,13(7):414.
37. Tanka K, Okam oto Y. A nasal aller gy model developed in the guinea pig by intr anasal applicat ion of 2, 4-to luene diiso cyanate [J]. I nt Ar chs Allergy Appl Immun,1988,85(4):392.
38.余洪猛,文三立,刘志刚,方勇,王华.豚草花粉变应原致过敏性鼻炎豚鼠模型的建立[J],上海实验动物科学,2001,21(4)：212-214.
39.余洪猛,文三立.蛔虫变应原致过敏性鼻炎豚鼠模型建立[J].上海实验动物科学,2000,20(4)：217-219.
40.黄山,许利平,周丽娟,等.鼻康胶囊治疗大鼠过敏性鼻炎的机理探讨[J].中成药,2006,28(4)：564-566.
41.张志明,孙树岩,季文樾.真菌性抗原致变应性鼻炎豚鼠模型建立仁[J].中国耳鼻咽喉头颈科,2005,12(5)：313-315.
42. Lange RW, etal [J]. ToxiCol SCi.1999;50(1):64-71.
43.张鑫悦,卢良,刘利兵,等.嶂螂致变应性鼻炎豚鼠模型的建立[J].第四军医大学学报,2009,30(3)：279-281.
44.陈子眠.抗过敏实验方法及过敏反应动物模型研究进展[J].云南中医学院学报.2000,23(3)：11-18.
45.胡艳菲,胡长庚,吴月琴,唐方.卵白蛋白诱发大鼠过敏性鼻炎模型不同方法的比较[J].吉林中医药.2011,31(10)：1023-1025.
46.章如新,江德胜,李兆基等.p物质能神经阻滞剂治疗变应性鼻炎的实验研究[J].中华耳鼻咽喉科杂志,1994,29(5)：282-285.
47.张德波,杨幼琪,冯诚,等.过敏反应与致敏剂量和致敏时间的关系研究[J].中国药品标准,2007(3)：20.
48.田道法.益气温阳活血法治疗变应性鼻炎.江苏中医药,2007,39(2)：3-4.
49.程坚,蔡外娇,张新民.中医药治疗变应性鼻炎辨证用药特点及分析.中华中医药杂志,2012,27(7)：1947-1950.
50.阮岩,陈蔚.王德鉴教授治疗鼻鼽经验介绍.新中医,2006,38(3)：12-13
51.严道南,吴继勇,马华安,周家璇,邢光前,郭小红,胡小燕,朱春晖.益气温阳方治疗变应性鼻炎肺脾虚寒证的临床研究.南京中医药大学学报,2012,28(6)：509-512.
52.杨占军,阮岩,廖榴业,王慧敏,张奕.温肾补阳法对变应性鼻炎患者转录因子T-bet/GATA-3表达的影响中医临床研究.2012,4(8)：6-7.
53.张世中,严道南.鼻敏温阳方治疗肾阳虚型变应性鼻炎20例疗效观察.云南中医中药杂志,2012,33(6)：29-30.
54.李淑良.从肾虚论治变应性鼻炎.江苏中医药,2007,39(2)：4
55.宋卫军,李湘,谢强.变应性鼻炎300例中医辨证分析.中国社区医师：医学专业,2012,14(8)：219.
56.陶敏慧,魏效峰,谢强,刘新红.谢强运用“温督祛霾”针灸疗法治疗变应性鼻炎的临床经验.中国民族民间医药杂志,2011,20(12)：138
57.王秀玲,胡金秀,谢强,黄冰林.应用谢强“升阳祛霾”法治疗变应性鼻炎60例临床观察.实用中西医结合临床.2009,9(6)：48-49.
58.苏妆.背俞穴主治规律分析[J].医学综述,2008,14(13)：2076-2078.
59.郑祥容,温木生.埋线疗法治百病[M].北京：人民军医出版社,2002.
60.韦玲菊,赵星星,杜艳.穴位埋线治疗变应性鼻炎的诊治概况[J].辽宁中医药大学学报,2009：11(12)：203-204.
61.华兴邦,李辞蓉,周浩良,等.大鼠穴位图谱的研制[J].实验动物与动物实验,1991,1：1-5.
62.Gawlik R, DuBuske L. Mediator release of neuroprptides after nasal provocation in perennial allergic rhinitis patients. Rhinology.2010,48(2):206-210.
63.Cima K, Voqelsinqer H, Kahler CM. Sensory neuropeptides are potent chemoattractants for human basophils in vitro.Requl Pept.2010,160 (1-3):42-48.
64. Pfaar O, Raap U, Holz M, et al. Pathophysiology of itching and sneezing in allergic rhinitis. Swiss Med Wkly.2009 24;139(3-4):35-40.
65. Sarin S, Undem B, Sanico A, et al. The role of the nervous system in rhinitis. J Allergy Clin Immunol. 2006,118(5):999-1016.
66. Heppt W, Dinh Q T, Cryer A. Phenotypic alteration of neuropeptide containing nerve fibres in seasonal intermittent allergic rhinitis. Clin Exp Allergy,2004,34:1105-1110.
67.杨旭东,孙光明,许学谷,等.组胺H3受体激动剂IMETIT对大鼠变应性鼻炎作用的初步探讨.临床耳鼻咽喉头颈外科杂志.2010,2(12)：559-563
68.陈金湘,杨瑞喜,黄之春.变应性鼻炎神经肽与肥大细胞关系的试验研究.临床耳鼻咽喉头颈外科杂志.2007,21(20)：947-949.
69.章如新,刘国钧,温武,等.P物质在变应性鼻炎中对一氧化氮作用的研究[J].第二军医大学学报,2005,26(6)：654-656.
70. Asadi S, Alysandratos KD, Angelidou A,et al. Substance P (SP) Induces Expression of Functional Corticotropin-Releasing Hormone Receptor-1 (CRHR-1) in Human Mast Cells. J Invest Dermatol. 2012,132 (2):324-329.
71.韩德民.变应性鼻炎[M].人民卫生出版社,第一版,2007：80.
72. Sergio Romagnani. The role of lymphocytes in allergic disease [J].Journal of Allergy and Clinical Immunology,2000,105(3):399.
73.席斌,田道法.变应性鼻炎肺气虚证与血清IL-4和IFN-γ相关性的实验研究.中国中西医结合耳鼻咽喉科杂志,2007,15(3)：166-170.
74. Bach JF.The effect of infectious on susceptibility to autoimmune and allergic diseases[J].N Engl J Med,2002,347(12):911-920.
75.Knipping S, Holzhausen HJ, Riederer A, et al.Allergic and idiopathic rhinitis:an ultrastructural study[J].Eur Arch Otorhinolaryngol,2009,266(8):1249-56.
76.Schaper C, Gustavus B, Koch B, et al.Effect of fluticasone on neuropeptides in nasal lavage in persistent allergic rhinitis[J].Investig Allergol Clin Immunol.2010,20(3):214-21.
77. Maes T, Tournoy KG, Joos GF.Gene Therapy for Allergic Airway Diseases[J]. Curr Allergy Asthma Rep.2011 Jan 18. [Epub ahead of print]
78.Chalastras T, et al. Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis:investigation using a liquid-based method[J]. Laryngol Otol.2008,122(7):700-6.
79. Durham SR. Gould HJ, Thienes CP, et al. Local control of e-gene expression in B cells of the mucosa in hayfever patients following allergen challenge[J]. Allergy C lin Immunol,1996,97(2):297-302.
80.许丽娟.变态反应性鼻炎的某些研究进展[J].中国中西医结合杂志,2002,10(1)：50.
81.黄少鹤,张棍生.变应性鼻炎患者白细胞介素4、γ干扰素水平与1gE的相关性研究[J].福建医药杂志,2004,26(2)：14-16.
82.李钦,杜英华,陈彦林等.鼻腔吸入IFN-γ治疗变应性鼻炎的实验研究.山东大学耳鼻喉眼学报,2007,21(1)：51-53.
83.刘国梁,施举红,林耀光,等T-bet基因修饰树突细胞对哮喘模型小鼠气道炎症的阻抑和逆转作用[J].中华医学杂志,2009,89(8)：519-523.
84.赵义造,黄益灯,黄加云等.电针对变应性鼻炎大鼠模型IL-4及IFN-γ表达的影响[J],浙江中医杂志,2010，45(12)：916-918.
85.Oboki K, et al.Th17 and allergy[J].Allergol Int.2008 Jun;57(2):121-34. Review.
86.Ciprandi G, et al. Peripheral Th-17 cells in allergic rhinitis:New evidence[J].Int Immunopharmacol. 2010;10(2):226-9.
87.Weaver CT, et al.Interplay between the TH17 and TReg cell lineages:a coevolutionary perspective[J].Nat Rev Immunol.2009 Dec;9(12):883-9. Review.
88. Eastaff-Leung N, et al. Foxp3+regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J].J Clin Immunol.2010;30(1):80-9.
89. ParkH,Liz, Yangxo.etal. A distinct lineage of CD4+T cells regulates tissue inflammation by Producing interleukinl7[J].Natlmmunol,2005,6(11):1133-1141.
90. Sallusto F, Lanzaveeehia A. Heterogeneity of CD4+memory T cells:functional modules for tailored immunity[J]. Eur J Immunol.2009;39:2076-2082.
91. StockingerB.VeldheenM. Dirgerentiation and function of Th 17 T cells. Curt OPin.ImmunoI, 2007,19:281-286.
92. Dong C.TH17 cells indevelopment:an updated view of their molecular identity and genetic programming[J]. Nat Rev Immunol.2008;8:337-348.
93. Mangan PR,Harrington LE,Quinn DB,et al.Transforming growth factor-beta induces development of the Th17 lineage[J].Nature,2006,441(7090):231-234.
94. Korn,Bettelli E.Oukka M, Kuehroo VK.IL-17 and Th17 Cells[J]. Arnu Rev Immunol.2009;27:485-517.
95. Nakae S,Nambu A,Sudo K,et al. Suppression of immune induction Of collagen-induced arthritis in IL-17-deficient mice[J].Immunol,2003,171 (11):6173-6177.
96. Koenders MI. Lubberts E, Oppers-walgreenb, et al. Blocking of interleukin-17 during reactivation o experimentalanhritis prevents joint inflammation and bone erosion by decreasing RANKL and interleukin-1[J].Am J Pathol,2005,167(1):141-149.
97.赵丽,马雄,邱德凯.Th17细胞/调节性T细胞平衡与自身免疫性肝病[J].胃肠病学,2009,4(6)：107-108.
98. Harrington LE,HattonRD,ManganPR,etal.Interzeukin17-produeingeD4+Effector T cells develop Via a lineage distinct from the T helper type 1 and 2 lineages[J]. Nature Immunol,2005,6(11):1123-1132.
99. Sakaguchi s, Sakaguchi N, Asano M, et al. Immunologic self-toleranee maintained by activated T cells expressing IL-2 receptoralpha-chains(CD25). Breakdown of a single mechanism of selftolerance causes various autoimmune diseases[J]. J Immunol,1995,155(3):1151-1164.
100. Nistala K,Moncrieffe H, Newton KR,Varsani H,,Hunter P, Wedderburn LR. Interleukin-17-Producing T cells are enriched in the joints of children with arthritis, but have a Reciprocal relationship to regulatory T cell numbers [J].Arthritis Rheum.2008:58:875-887.
101. Moses H L,Branum E L,Proper J A,et al.Transforming growth factor production by chemically transformed cells[J]. Cancer Res,1981,41(7):2842-2848.
102.陈芳,李晓艳T-bet、TGF-β在过敏性鼻炎中的研究进展[J].山东大学耳鼻喉眼学报,2012,26(2)：83-85.
103. Karagiannidis C,Akdis M,Holopainen P,et al.Glucocorticoids up-regulate FOXP3 expression and regulatory T cells in asthma[J] J Allergy Clin Immunol,2004,114(6):1425-1433.
104.韩德民主编,副主编张罗,董震,王德云,林小平等.变应性鼻炎,人民卫生出版社2007：149.
105. Chen C H,Seguin-Devaux C,Burke N A,et al.Transforming growth factor beta blocks Tec kinase phosphorylation, Ca2+ influx, and NFATc translocation causing inhibition of T cell differentiation[J]. Exp Med,2003,197(12):1689-1699.
106. Scherf W,Burdach S,Hansen G. Reduced expression of transforming growth factor beta 1 exacerbates pathology in an experimental asthma model[J]. Eur J Immunol,2005,35(1):198-206.
107. Redington AE, Roche WR, Holgate ST, et al. Co-localization of immunoreactive transforming growth factor-1 and decorin in bronchial biopsies from asthmatic and normal subjects[J].J Pathol. 1998,186:410-415.
108. Blobe GC, Schiemann WP, Lodish HF. Role of transforming growth factor-in human disease[J]. N Engl J Med.2000;342:1350-1358.
109. Salib R J,Howarth P H.Transforming growth factor-beta in allergic inflammatory disease of the upper airways:friend or foe?[J].Clin Exp Allergy,2009,39(8):1128-1135.
110.Olsson N,Piek E,ten Dijke P,et al. Human mast cell migration in response to members of the transforming growth factor-beta family[J].J Leukoc Biol,2000,67(3):350-356.
111. Ouyang Y,Miyata M,Hatsushika K,et al. TGF-beta signaling may play a role in the development of goblet cell hyperplasia in a mouse model of allergic rhinitis[J]. Allergol Int,2010,59(3):313-319.
112. Barth RF. Boron neutron capture therapy at the crossroads:challengesand opportunities[J].Appl Radiat Isot,2009,67(7-8):S3-6.
113. Taylor A, Verhagen J, Akdis C A, et al. T regulatory cells in allergy and health:a question of allergen specificityand balance[J]. Int Arch Allergy Immunol,2004,135(1):73-82.
114. V ignola AM, Chan ez P, Ch iappara G, et al.Transforming growth factor-beta express ion in m ucosal biopsies in asthma and chronic bronchitis. J Am JRespir C ritC areMed,1997;156:591-9.
115. Pudd icombe SM, Polosa R, R ichterA, et al. Involvement of the epidermal growth factor receptor in epithelial repair in asthma. J FASEB J,2000; 14:1362-74.
116. Kenyon NJ, Ward RW, Grew GM,et al.TGF-1 causes airway fibrosis and increased collagen Ⅰ and Ⅲ mRNA in mice. Thorax.2003;58:772-777.)
117.刘争,陆翔,王恒.转化生长因子β1白细胞介素-6及11和17在变应性鼻炎患者鼻黏膜中的表达.临床耳鼻咽喉科杂志,2006,20(14)：625-627.
118.雷霏、许晶,徐洪君,等.实验性变应性鼻炎豚鼠鼻黏膜中TGF-β1表达及意义[J].中国老年学杂志2008,28(6)：533-534.
119. Zhang H, Phan S. Inhibition of myofibroblast apoptosis by transforming growth factor-1. Am J Respir Cell Mol Bio.1999;21:658-65.
120.于睿莉,朱冬冬,董震.中华耳鼻咽喉头颈外科杂志.2006；41(10)：773-776.
121. Lee, SS,Won, TB.Kim, JW,et al. Effects of Dexamethasone on the Expression of Transforming Growth Factor-[beta] in the Mouse Model of Allergic Rhinitis [J]. Laryngoscope.2007;117(8): 1323-1328.
122.杨长树.曲安奈德鼻喷雾剂治疗儿童过敏性鼻炎的临床疗效观察.中国实用医学,2011,6(25)：129-130.
123.介华.曲安奈德鼻喷雾剂在过敏性鼻炎中的临床应用.中国实用医学,2012,7(21)：183-184.
124.崔雪峰,田建华.曲安奈德鼻喷雾剂治疗变应性鼻炎的临床观察.承德医学院学报,2010,27(1)：18-19.
125.谷小鸣,方薇,方润琪.曲安奈德鼻喷雾剂和伯克纳喷鼻剂治疗过敏性鼻炎的平行对照试验.中华临 床医学杂志,2004,5(7)：45-46.
1.王士贞.中医耳鼻咽喉科学[M].北京：国中医药出版社,2006：124.
2.刘兆琳,侯慎英,赵立新.过敏煎治疗变应性鼻炎90例[J].河北中医,2005,27(3)：227.
3.朱正民,耿以安,陈虹.小青龙汤加减治疗过敏性鼻炎[J].湖北中医杂志,2004,26(5)： 38-39.
4.贾德蓉.浅谈“从肝入手、分期论治变应性鼻炎”[J].中医耳鼻喉科学研究杂志,2010,9(1)：32-33.
5.郭强中,李云英.柴胡桂枝汤治疗过敏性鼻炎经验[J].中医药报,2008,7(4)：53-54.
6.彭建中.清晨发病从肝论治验案举隅[J].北京中医药大学学报,2002,25(6)：66.
7.张士国.复方辛黄滴鼻液治疗鼻炎1637例疗效观察[J].山东中医杂志,2001,20(5)：
34-38
8.郑昌雄.中医耳鼻咽喉科学[M].上海：上海科技教育出版社,第一版.1995.
9.彭向军.中药加塞鼻剂治疗过敏性鼻炎40例体会[J].甘肃中医,2002,(4)：25.
10.赵义造,黄益灯,黄加云等.电针对变应性鼻炎大鼠模型IL-4及IFN-γ表达的影响[J].浙江中医杂志,2010,45(12)：916-918.
11.王静云.电针对过敏性鼻炎动物模型的干预作用及其免疫学机制研究.博士论文.2005年,辽宁中医药大学.
12.何天有,李惠琴,赵耀东,等.透刺为主治疗过敏性鼻炎60例[J].中国针灸,2006,26(2)：110.
13石学敏.针灸学[M].北京.中国中医药出版社.2002.
14.杨冠军,刘艳丽,许卫国.隔姜灸背俞穴治疗过敏性鼻炎60例[J].中国针灸,2001,21(3)：148.
15.胡志平,李小军,黄克伟.隔附子饼灸治疗过敏性鼻炎82例[J].上海针灸杂志,2005,24(9)：39.
16.周琦,曹明根,张雷.赵氏灸法治疗常年性过敏性鼻炎70例[J].上海中医药杂志,2001,35(9)：27.
17.寻满湘.穴位注射治疗变应性鼻炎[J].中国中西医结合耳鼻咽喉科杂志,2002(4)：180-181.
18.刘明清,谢小明,刘俐,等.穴位注射治疗变应性鼻炎的疗效观察[J].上海针灸杂志,2006(8)：10-11.
19.杨杰.卡介菌多糖核酸穴位注射辅助治疗常年性变应性鼻炎的疗效观察[J].中医药临床杂志,2009(6)：539-540.
20.郑祥容,温木生.埋线疗法治百病[M].北京：人民军医出版社,2002.
21.韦玲菊,赵星星,杜艳.穴位埋线治疗变应性鼻炎的诊治概况[J].辽宁中医药大学学报,2009：11(12)：203-204.
22.田雪秋,席中原,牟开金.穴位埋线治疗过敏性鼻炎50例[J].中医外治杂志,2011,20(6)：23.
23.邹昆.穴位埋线配合艾灸治疗变应性鼻炎疗效观察.中医药临床杂志,2011,23(7)：599-600.
24.秦尔奇,苑家敏.穴位敷贴治疗变应性鼻炎研究进展[J].中医研究,2012,25(2)：74-76.
25.陈传江,吴强,林栋.经络假说与穴位贴敷作用机理探讨[J].福建中医学院学报,2006,16(1)：26—28.
26.郎笑梅.夏季穴位敷贴治疗过敏性鼻炎30例[J].中医药临床杂志,2007,19(1)：31-32.
27.罗开涛,边晓东.穴位贴敷治疗过敏性鼻炎60例[J].浙江中医杂志,2011,46(1)：23-24.
28.刘光荣.内服中药苍耳辛夷散外加穴位按摩治疗过敏性鼻炎39例[J].按摩与导引,2004,20(04)：26.
29.赵国瑞,赵长云,林禄.中药滴鼻配合穴位按摩治疗过敏性鼻炎106例疗效观察[J].中国中医药科技,2002,9(2)：119.
30.李漫.耳穴贴压法治疗变应性鼻炎的疗效观察[J].医学综述2007,13(23)：1888-1889.
31.唐泳华.王不留行耳穴贴敷治疗变应性鼻炎[J].中国民间疗法,2001,9(02)：25.
32.齐伟,王朝辉.耳穴贴压治疗过敏性鼻炎50例临床观察[J].长春中医药大学学报,2008,24(6)：721.
33.朱向阳,邓耀成.耳穴药物注射法治疗过敏性鼻炎[J].针灸临床杂志,2000,16(06)：46-47.
34.吴震西,张锦秀.发泡膏贴治过敏性鼻炎64例[J].中医杂志,1988,09：44.
35.汤之明.斑鳌浸出液发泡治疗常年性变态反应性鼻炎的疗效及其血清总IgE的形响[J].中国中西医结合杂志,1995,15(6)：334.
36.杜昌华.耳背刺血治疗过敏性鼻炎60例[J],实用中医药杂志,1998,14(7)：37.
37.姚靳里拔罐疗法治疗常年性变态反应性鼻炎68例小结[J]中国中西医结合耳鼻咽喉科杂志,2008(5)：211.
38.孔维佳,周梁,许庚等.耳鼻咽喉头颈外科学[M].北京：人民卫生出版社,2005：75.
39.孔维佳,等.耳鼻咽喉头颈外科学[M].第二版.北京：人民卫生出版社,2010：288.
40. Leung R, Ho P. Asthma, allergy, and atopy in the three southeast Asian population[J]. Thorax.1994;49:1205-1210.
41. Celedon JC, Palmer LJ. Weiss ST, et al. Asthma, rhinitis, and skin test reactivity to aerollergens in families of asthmatic subjects in Anqing, china[J]. Am J Respir Crit Care Med.2001:163:1108-1112.
42.中华医学会耳鼻咽喉科学分会,中华耳鼻咽喉科杂志编辑委员会.变应性鼻炎诊断及疗效评定标准[J].中华耳鼻咽喉科杂志.1998：33：134-135.
43. Housquet J, Aahl R, Khaltaev N. Global alliance chronic reapiratory disease [J]. Allergy,2007,62:216-223.
44. BousquetJ, vanCauwenbergeP, KhaltaevN. Allergic rhinitis and its impact on asthma[J].J Allergy Clinlmmunol,2001,108(5):147-334.
45. Bousquet J, Khaltaev N, Cruz AA, et al. Allergic Rhinitis and its Impact on Asthma (ARIA) 2008 update(in collaboration with the World Health Organization, GA2LEN and AllerGen) [J]. Allergy,2008,63:S8-160.
46. Brozek JL, Bousquet J, Baena-Cagnani CE, et al. Allergic Rhinitis and its Impact on Asthma (ARIA)guidelines:2010 Revision [J]. J Allergy Clin Immunol, 2010,126:466-476.
47.孔维佳,周梁,许庚等.耳鼻咽喉头颈外科学[M].北京：人民卫生出版社,2005：75-76
48.中华耳鼻咽喉头颈外科杂志编委会鼻科组。变应性鼻炎诊断和治疗指南解读[J].中华耳鼻咽喉头颈外科杂志,2009,44(12)：972-976.
49.韩德民主编.变应性鼻炎[M].人民卫生出版社,2007,109-117。
50.张罗,周兵,韩德民,等.变应性鼻炎研究进展(一)：发病机制[J].耳鼻咽喉：头颈外科,2003,10(5)：316-320.
51. Strachan DP. Hayfever, hygiene and house hold size[J].BVJ,1989; 299:1259-1260.
52.Strachan DP. Familysize, infection and atopy:the first decade of the "hygiene hypothesis" [J]. Thorax,2000;55(Suppl1)2-10.
53. Bachert C, Gevaert P, van Cauwenberge P. Staphylococcus aureus enterotoxin:a key in airway disease? [J]. Allergy,2002,57(6):480-487.
54.孔维佳,周梁,许庚等.耳鼻咽喉头颈外科学[M].北京：人民卫生出版社,2005：77
55. Shiomori T, Yoshida S, Miyamoto H, Makishima K. Relationship of nasal carriage of Staphylococcus aureus to pathogenesis of perennial allergic rhinitis [J]. J Allergy Clin Immunol,2000,105(3):449-454.
56. Rossi RE, Monasterolo G. Prevalence of serum IgE antibodies to the Staphylococcus aureus enterotoxins(SEA, SEB, SEC, SED, TSST-1) in patients with persistent allergic rhinitis [J]. Int Arch Allergy Immunol,2004,133 (3):261-266.
57. Holt PG, Macaubas C, Stumbles PA, et al.The role of allergy in the development of asthma. Nature.1999;402:12-17.
58.孔维佳,周梁,许庚等.耳鼻咽喉头颈外科学[M].北京：人民卫生出版社,2005：77
59. Vamey VA, Jacobson MR, Sudderick RM, et al. Immuunohistilogy of thenasal mucosa following allergen-induced rhinitis. Identification of activated T lymphocytes, eosinophils, and neutrohpils. Am Rev Respire Dis.1992, 146:170-176.
60.Robinson DS, Hamid G, Ying S, et al. Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma[J]. NEng J Med.1992,326:298-304
61. Knipping S, Holzhausen HJ, Riederer A, et al. Allergic and idiopathic rhinitis: an ultrastructural study[J].Eur Arch Otorhinolaryngol, 2009,266(8):1249-56.
62.Schaper C, Gustavus B, Koch B, et al. Effect of fluticasone on neuropeptides in nasal lavage in persistent allergic rhinitis[J].J Investig Allergol Clin Immunol.2010,20(3):214-21.
63.Weaver CT, et al. Interplay between the TH17 and TReg cell lineages:a (co-)evolutionary perspective[J]. Nat Rev Immunol.2009;9(12):883-9.
64. Eastaff-Leung N, et al. Foxp3+ regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J].J Clin Immunol.2010 Jan; 30(1):80-9.
65.Maes T, Tournoy KG, Joos GF.Gene Therapy for Allergic Airway Diseases[J]. Curr Allergy Asthma Rep.2011 Jan 18. [Epub ahead of print]
66. Chalastras T, et al. Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis:investigation using a liquid-based method[J].J Laryngol Otol. 2008;122(7):700-6.
67. Bach JF. The effect of infectious on susceptibility to autoimmune and allergic diseases[J]. N Engl J Med,2002,347(12):911-920.
68.Kay AB. Allergy and allergic diseases:first of two parts[J]. NEngl J Med,2001,344(1):303713.
69.Oboki K, et al.Th17 and allergy[J]. Allergol Int.2008 Jun;57(2):121-34. Review.
70.Ciprandi G, et al. Peripheral Th-17 cells in allergic rhinitis:New evidence[J]. Int Immunopharmacol.2010;10(2):226-9.
71. Weaver CT, et al. Interplay between the TH17 and TReg cell lineages:a coevolutionary perspective[J]. Nat Rev Immunol.2009;9(12):883-9. Review.
72. Eastaff-Leung N, et al. Foxp3+regulatory T cells, Th17 effector cells, and cytokine environment in inflammatory bowel disease[J].J Clin Immunol. 2010;30(1):80-9.