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画眉草弯孢霉菌株QZ-2000与马唐互作及其影响因素的研究
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摘要
马唐属(Digitaria)杂草广泛分布于世界的热带和温带地区,是危害玉米、棉花、大豆、高粱、花生、甘蔗、烟草等秋熟旱作田的世界性恶性杂草。目前对马唐属杂草主要通过人工、机械和化学的方法进行防除,但效果并不十分理想,并且带来很大的负面影响。画眉草弯孢霉(Curvularia eragrostidis J. A. Meyer)属于真菌界、半知菌类、腔菌目、腔菌科、弯孢霉属(Curvularia Boedijn.),画眉草弯孢霉菌株QZ-2000作为新开发的生物除草剂——敌散克(Disancu)的马唐致病菌,对马唐具有较强的致病性。此前的相关研究已经涉及到菌株QZ-2000的生物学、大批量生产工艺、剂型研制、寄主范围、室内和田间除草剂效果评价等方面。但是,有关该真菌与寄主间的相互作用的研究较少,这种互作关系可以揭示画眉草弯孢霉菌株QZ-2000对马唐防控的机制,而这正关系到最终的防除效果和产品的稳定性。因此,本文围绕画眉草弯孢霉菌株QZ-2000与防治对象马唐的相互作用,开展侵染过程中防除对象的反应机制和大批量生产方法以及孢子贮藏对产品效果影响的研究,为研发效果稳定的敌散克产品提供理论基础。
     本文首先介绍了画眉草弯孢霉菌株QZ-2000与马唐互作的生理生化、叶绿素荧光动力学参数的变化和基因差异表达,揭示了两者的互作机制;最后讨论了画眉草弯孢霉菌株QZ-2000孢子活力的影响因素,提出了保持和提高孢子致病力的方式,为发挥画眉草弯孢霉菌株QZ-2000在与马唐互作中的效力、开发生物除草剂奠定基础。
     使用画眉草弯孢霉菌株QZ-2000孢子悬浮液喷施马唐幼苗,从处理后第一天开始,连续测定马唐叶绿素、可溶性蛋白和丙二醛的含量;并利用聚丙烯酰胺凝胶电泳适时测定酯酶同工酶。与对照相比,各处理马唐叶片的叶绿素和可溶性蛋白含量呈下降趋势,丙二醛含量呈上升趋势;酯酶同工酶带也有差异。孢子悬浮液与低剂量化学除草剂复配处理也有类似效果,马唐叶绿素和可溶性蛋白含量下降幅度和丙二醛含量上升幅度最大,低剂量化学除草剂处理的三种指标变化幅度最小。真菌孢子在与杂草互作中的强弱是相对的,与低剂量化学除草剂复配可以增强画眉草弯孢霉菌株QZ-2000孢子的致病力,使其在与马唐互作中占有优势。表明画眉草弯孢霉菌株QZ-2000孢子以及与低剂量化学除草剂复配,对马唐幼苗的生长抑制效果最佳。荧光动力学研究显示,画眉草弯孢霉菌株QZ-2000与化学除草剂复配或使用画眉草弯孢霉菌株QZ-2000悬浮液单独处理,均可降低马唐光合系统原初光化学反应的最大量子速率、光合系统Ⅱ光化学最大效率、阻碍光合作用过程中的电子传递,进而降低马唐的光合作用。
     利用cDNA-AFLP技术对画眉草弯孢霉菌株QZ-20005×105,1×106两种浓度的孢子液和12h,24h两种保湿时间条件下侵染马唐的基因表达谱进行分析。结果表明,接种高浓度的孢子对马唐的致病性更强。利用256对选择性扩增扩引物组合共获得了差异条带1214条,其中有518个上调表达的转录衍生片段。对其中的46条进行克隆,测序,得到35个TDF,其中25个序列在Blastx GenBank非冗余的公共序列数据库检索出同源序列,有6个是上调基因,参与了信号转导、能量代谢、细胞生长和发育、应激反应、脱落酸生物合成和应激。这些反应是马唐被画眉草弯孢霉菌株QZ-2000侵染后致病的重要途径。研究结果为进一步研究画眉草弯孢霉菌株QZ-2000侵染马唐的发病机理和作为生物除草剂的致病菌防除马唐提供了基础。
     保持和提高孢子的活力可以增强其致病力,探讨影响孢子活力的因素,明确保持孢子致病力的方式是成功开发生物除草剂的关键之一。研究了画眉草弯孢霉孢子的传代次数和核酸含量与贮藏时间的关系以及对孢子活力的影响。在4℃冰箱保藏期1-8年间,随着保藏时间的延长,RNA含量的变化幅度大于DNA,两者的含量均呈现下降趋势,其萌发率和致病力也随着贮藏时间的延长和核酸含量的下降而降低。贮藏3年,孢子萌发率不足20%;贮藏4年,萌发率降至10%以下,致病力极低,基本失去致病作用;贮藏5年失去致病力;贮藏6年孢子失去活力,不能萌发。在20代以内,画眉草弯孢霉菌株QZ-2000随着传代次数增加,孢子萌发率、萌发芽管长度和致病力呈现下降趋势,但差异不明显。研究了杂粮作为固体基质以及改进生产方式对提高孢子活力的影响。与麦麸等固体培养基比较,所供试验的杂粮克产孢量普遍较高,其中以小米的克产孢量最高;改进孢子培养方式,对孢子产量、萌发芽管长度等有明显促进作用,但对孢子萌发率的促进效果不显著。综合产孢量、萌发率及萌发芽管长度三个方面的因素,小米是马唐生防菌株QZ-2000产孢最适培养基组分。
     为了了解孢子的形态与萌发的关系,观察了画眉草弯孢霉菌株QZ-2000的孢子,用苯胺蓝染色后进行显微观察,每个孢子由四个部分组成,中部两部分较大,染色较浅,表明具有丰富的原生质;两端的两部分小而圆尖,染色较深,表明原生质含量较少。两者之间由通道相连,孢子的两端部各有一个孔口,芽管由此萌发孔伸出。中部原生质通过通道经两端向外伸出萌发芽管。这种结构对理解孢子的形态特点具有一定意义。
Curvularia eragrostidis J. A. Meyer, a species of fungi, belongs to family Dematiaceae, Moniliales, Deuteromycetes. Its strain QZ-2000as a key component of Disancu, a newly-developed bioherbicide, has a virulent pathogenicity to Large crabgrass (Digitaria sanguinalis (L.) Scop.).Large crabgrass, one of the worst weeds in dryland agricultural areas, is distributed throughout tropical and temperate regions of the world and is ranked fourth on a list of serious weeds in these regions. The manual, mechanical and chemical control techniques have been developed for this weed, but the successful results was not made yet. The previous studies have involved in biological characteristics, mass-production techniques, formulation, host range and effectiveness bioassay in greenhouse and fields for the strain QZ-2000. The interaction between the strain and host has not been fully studied, which may link to improve the stability and efficacy of this bioherbicide product. Therefore, the interaction of the strain QZ-2000and the target Large crabgrass is studied to provide theoretical basis for a development of Disancu product. Those studies include influencing factors in conidial viability through improvement of the mass-production of conidia of C. eragrostidis strain QZ-2000and storage, and synergy of low-rate herbicide and responses of the host to the inoculum infection.
     The physiological indexes such as chlorophyll content, soluble protein and MDA and enzymes in large crabgrass were tested after treatment of conidial suspension of C. eragrostidis, low dosage chemical herbicide alone and their mixture. The treatment of C. eragrostidis strain QZ-2000mixtured with low dosage chemical herbicide caused the maximum reduction of chlorophyll content and soluble protein and maximum increase of MDA content. The esterase isozymes demonstrated various activities in treated large crabgrass plants by polyacrylamide gel electrophoresis. The results indicate that C. eragrostidis strain QZ-2000working with low dosage chemical herbicide enhanced inhibition of large crabgrass seedling growth. The fluorescence kinetics parameters demonstrated that treatment of conidial suspension of C. eragrostidis alone or combination with low-rate herbicides caused Large crabgrass to reduce the PHI(PO) of PS Ⅱ and its activity and impede electron transfer and finally to decrease photosynthesis rate.
     Gene expression profiles of large crabgrass infected by C. eragrostidis strain QZ-2000at the treatment regime at two concentrations of conidia and two dew durations were analyzed by cDNA amplified fragment length polymorphisms (cDNA-AFLP). Inoculum strength was more determinant of gene expression than dew duration. A total of256primer combinations were used for selective amplification and1214transcript-derived fragments (TDFs) were selected for their differential expression. Of these,518up-regulated differentially expressed TDFs were identified. Forty-six differential cDNA fragments were chosen to cloned and35of them were successfully cloned and sequenced, of which25were homologous to genes of known function according to the GenBank database. Only6genes were up-regulated in C. eragrostidis-inoculated large crabgrass, with functions involved in signal transduction, energy metabolism, cell growth and development, stress responses, abscisic acid biosynthesis and response. It appears that a few pathways may be important parts of the pathogenic strategy of C. eragrostidis strain QZ-2000on large crabgrass. Our study provides the fundamentals to further study the pathogenic mechanism, screen for optimal C. eragrostidis strains as potential mycoherbicide and apply this product to control Digitaria sanguinalis.
     Fungal spore has the advantages of highly virulence and easy to storage. Ingredient of solid substrate had a great effect on the spore quantity of Curvularia eragrostidis strain QZ-2000. Coarse cereals were evaluated as better solid substrates for sporulation of C. eragrostidis compared to sideline products. Sporulation, length of gemma of conidia was increased obviously and germination rate unobviously with improved culture mode. The millet seed as substrate improved the sporulation. Germination rate, length of gemma and pathogenicity of conidia was gradually decreased with the serial passage generation increased in20generation, but the difference was insignificant. The relationship between the DNA and RNA content of C. eragrostidis conidium and its storage duration was studied. During1-8years' storage, the DNA and RNA content of C. eragrostidis conidium decreased with storage duration extension. The bigger reduction happened in the RNA content than in the DNA content. In20generations of successive transfers of culture, there was a reducing trend in germination rate, tube length and pathogenicity of conidia with a serial passage generation extension despite insignificant difference.
     Conidia of C. eragrostidis strain QZ-2000stained with aniline blue were observed under a light microscope. The results demonstrated that a conidium was composed of four cells. The bigger two cells with full of protoplasm located in the middle. One cell each pole had a germ pore at end respectively. The protoplast within the bigger cells connected to that with the smaller cells through a thin channel. Germination tube grew out of one or both of the small cells.
引文
1. Agarwal S K, Jin K L. Roll of small RNAs in host-microbe interactions. Annual Review of Phytopathology,2010,48:225-246.
    2. Alam M, Sattar A, Chauahuri P K, Janardhanan K K and Husain A. Isolation purifiction and characterization of a phytotoxin produced by Curvularia andropogonis. Plant Science Limerrick, 1977,123:47-55.
    3. Altieri M A. Liebman weed manegementin agroecosystems. CRC Press, Inc. Florida,1988
    4. Altman J and Campbell C L. Effects of herbicides on plant diseases. Annual Review of Phytopathology,1977,15:361-385.
    5. Amsellem Z, Zidack N K, Charles Quimby Jr P, and Gressel J. Long-term dry preservation of viable mycelia of two mycoherbicidal organisms. Crop Protection,1999,18:643-649.
    6. Amsellen Z, Sharon A, Gressel J. Abolition of selectivity of two mycoherbicidal organisms and enhanced virulence of a virulent fungus by an invert emulsion. Phytopathology,1991,81:925-929.
    7. Amsellen Z B, Cohen B A and Gressel J. Engineering hypervirulence in a mycoherbicidal fungus for efficient weed control. Nature Biotechnology.2002,20:1035-1039.
    8. Armond P A, Bjorkman O, Staehelin L A. Dissociation of supramolecular complexes in chloroplast membranes A manifestation of heat damage to the photosynthetic apparatus. Biochimica et BiophysicaActa (BBA)-Biomembranes,1980,601:433-442.
    9. Ash G J. The science, art and business of successful bioherbicides. Biological Control,2010,52(7): 230-240.
    10. Auld B A, Smith H E, Qiang S. Control of cocklebur with a combination of Alternaria zinniae and low rates of imazaquin [A]. In:Anonymous ed. Proceedings of the 16thAsian2Pacific weed science society conference [C]. Mylaysia:Asian-Pacific Weed Science Society,1997.345-357.
    11. Auld B A. Economic aspects of biological weed control with plant pathogens [A]. In:TeBeest D O. ed. Microbial control of weed [C]. New York:Chapman and Hall,1991.262-273.
    12. Auld B A. Hetherington S D, Smith H E. Weed Biology and Management,2003,3(2):61-67.
    13. Bailey K L, Boyetchko S M, Langke T. Social and economic druvers shaping the future of biological control:A Canadian perspective on the faceors affecting the development and use of microbial biopesticedes. Biological Control,2010,52(7):221-229.
    14. Bannon J S. CASSTTM herbicide (Alternaria cassiae):a case history of a mycoherbicide. American Journal of Alternative Agriculture,1988,2:73-76.
    15. Bassam B J, Caetano-Anolles G, Gresshoff P M. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry,1991,196:80-83.
    16. Bachem CWB, Oomen RJF, Visser RGF. Transcript imaging with cDNA-AFLP:a step-by-step protocol. Plant Molecular Biology Reporter,1998,16:157-157.
    17. Blackshaw R E. Manage weed in conservation tillage. Proceedings In Lethbridge Reduced Tillage Conference,1995:30-32.
    18. Boyetchko S M. Principles of biological weed control with microorganism. Hort Science.1997, 32(2):201-205.
    19. Boyette C D, Abbas H K, and Connick W J. Evaluation of Fusarium oxysporum as a potential bioherbicide for sicklepod(Cassia obtusifolia), coffee senna(C. occidentalis), and hemp sesbania(Sesbania exaltata). Weed Science.1993,41:678-681.
    20. Boyette C D, Quimby P C, Connick W J Jr, Daigle D J and Fulgham F E. Progress in the production, formulation, and applicationof mycoherbicides. in TeBeest D O, ed. Microbial Control of weeds. Chapman and Hall, New York,1991,209-222.
    21. Boyette C D, Templeton G E, andOliver L R. Texas gourd (Cucubita texana) control with Fusarium solani f.sp. cucurbitae. Weed Science.1985,32:649-654.
    22. Boyette CD, Hoagland RE, Weaver MA. Biocontrol efficacy of Colletotrichum truncatumforhemp sesbania (Sesbania exaltata) is enhanced with unrefined corn oil and surfactant. Weed Biology and Management,2007,7(1):70-76.
    23. Browers R C. Commercialization of CollegoTM-an industrialist's view. Weed Science.1986,34: 24-25.
    24. Cardina J, Litrell R H, and Hanlin R. Anthracnose of Florida beggarweed (Desmodium tortuosum) caused by Colletotrichum truncatum. Weed Science.1988,36:329-334.
    25. Chandramohan S, Charudattan R. Control of seven grasses with a mixture of three fungal pathogens with restricted host ranges. Biological Control.2001,22:246-255.
    26. Charudattan R and Dinoor A. Biological control of weeds using plant pathogens accomplishments and limitations. Crop Protection,2000,19:691-695.
    27. Charudattan R, Linda S B, Klupfel M and Osman A. Biocontrol efficacy of Cercospora rodmanii on waterhyacinth. Phytopathology,1985,75:1263-1269.
    28. Charudattan R. The mycoherbicide approach with plant pathogens. In TeBeest D O. Microbial control of weed. New York Chapman and Hall.1991,24-57.
    29. Charudattan, R. Integrated control of wateryacinth(Eichhornia crassipes) with a pathogen, insects, and herbicides. Weed Science.1986,34(Suppl.1):26-30.
    30. Charudattan, R. Integrated control of wateryacinth (Eichhornia crassipes) with a pathogen, insects, and herbicides. Weed Science.1986,34(Suppl.l):26-30.
    31. Chen J, Lin R M, Gao Z G. The influence of Curvularia lunata toxin on defense enzyme activities and its function in the induced resistance of corn. Acta Phytopathologica Sinica.2002,32:43-48.
    32. Chen S G, Dai X B, Qiang S, et al. Action of tenuazonic acid, a natural phytotoxin, on
    photosystem II of apinach. Environmental and Experimental Botany,2008,62:679-689.
    33. Chen S, Yin C, Oiang S, et al. Chloroplastic oxidative burst induced by tenuazonie acid, a natural photosynthesis inhibitor, trig gem cell nectosis in Eupatorium adenophorum Spreng. Biochimica et Biophysica Acta-Bioenergetics,2010,1797:391-405.
    34. Connick W J Jr, Boyette C D, and McAlpine J R. Formulation of myco-herbicides using a pasta-like process. Biological Control.1991,11:281-287.
    35. Daigle D J, Connnick W J Jr. Formularting mycoherbicides. In:The Mycota,X Industrial Applacations(ed. By Osiewacz A.D.). Springer Verlag, Berlin,2002:275-388.
    36. Daigle D J, Connick W J Jr. Formulation and application technology for microbial weed control.In:Hoagland,R.E.(Ed.),Micribes and Microbial Products as Herbicides. American Chemical Society, Washington, DC.1990,288-304.
    37. Davies K A,Deloronoil I, Foster S J, Lli D, Johnstone K, Ashby A M, Evidence for a role of cutinase in pathogenicity of Pyrenopeziza brassicae on brassicas. Physilological arid Molecular Plant Pathology,2000,57:63-75
    38. Dayan FE, Duke S O, Grossmann K. Herbicides as probes in plant biology. Weed Sicence,2010, 58:340-350.
    39. Duke S O, Powles S B. Glyphosate:a once-in-a-century herbicide. Pest Management Science,2008, 64:319-25.
    40. Duke S O. Herbicides and pharmaceutical relationships. Weed Sicence,2010,58(7):334-339.
    41. Eduardo E. Trujillo. History and success of plant pathogens for biological control of introduced weeds in Hawaii. Biological Control,2005,33:113-122.
    42. Egley G H, Hanks J E, and Boyette C D. Invert emulsion droplet size and myco-herbicidal activity of Colletotrichum truncation. Weed Technology,1993,7:417-424.
    43. Elzein A, Kroschel J. and Dorette Miiller-Stover D M, Effects of inoculum type and propagule concentration on shelf life of Pesta formulations containing Fusarium oxysporum Foxy 2, a potential mycoherbicide agent for Striga spp. Biological Control,2004, (30)2:203-211.
    44. Eyherabide J J, Calvino P A, Forcella F, Gabriela C, Eradat Oskoui K. Solaria help predict in-crop weed densities. Weed Technology,2003,17(1):166-172.
    45. Fan C Y, Wolfram Koeller W. Diversity of cutinases from plant pathogenic fungi; differential and sequential expression of cutiaolytic esterases by Alternaria brassicicola. FEMS Microbiology Letters,1998,158:33-38.
    46. Gaines T A, Zhang W, Wang D, Stephen T C and Dale L S. Gene amplification confers glyphosate resistance in Amaranthus palmeri. Proceedings of the National Academy of Sciences,2010,107: 1029-1034.
    47. Geng R M, Zhang J P, Yu L Q. Helminthosporium gramineum Rabehn f. sp. Echinochloae conidia for biological control of barnyardgrass. Weed Sicence,2009,57:554-561.
    48. Ghorbanl R, Seel W, Litterick A and Leifert C. Evaluation of Alternaria alternata for biological control of Amaranthus retroflexus. Weed Science,2000,48:474-480.
    49. Gonzalez C V, Ibarra S E, Piccoli P N, Botto J F and Boccalandro H E. Phytochrome B increases drought tolerance by enhancing ABA sensitivity in Arabidopsis thaliana. Plant, Cell Environment, 2012,35:1958-1968.
    50. Goodwin P H. A molecular weed-mycoherbicide interaction:Colletotrichum gloeosporioides f. Sp. malvae and round-leaved mallow, Malva pusilla. Canadian Journal of Plant Pathology.2001, 23:28-35.
    51. Guo J, Jiang R H, Kamphuis L G, Govers F. A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans. Fungal Genetical Biology.2006,43:111-123.
    52. Hetherington S D, Smith H E, Scanes M G and Auld B A. Effects of some environmental conditions on the effectiveness of Drechslera avenacea (Curtis ex Cooke) Shoem:a potential bioherbicidal organism for Avenafatua L. Biological Control,2002,24:103-109.
    53. Hoagland R E. Chemical interaction with Bioherbicides to improve efficacy. Weed Technology, 1996,10:651-674.
    54. Hoagland R E, Boyette C D, Abbas HK. Myrothecium verru-cariaisolates and formulations as bioherbicide agents forkud-zu. Biocontrol Science and Technology,2007,17(7):721-731.
    55. Holm L G, Plucknett D L, Pancho J V, Gerberger J P. The World's Worst weeds Distribution and Biology.1977, The University Press of Hawaii, Honolulu, Hawaii, USA.
    56. Huber L. Modelingleaf wetness in relation to plant disease epidemiology. Annual Review of Phytopathology.1992,30:553-577.
    57. Hynes R K, Chumala P B, Hupka D., Peng G. A complex coacervate formulation for delivery of Colletotrichum truncatum 00-003B1. Weed Technology,2010,24:185-192.
    58. Jensen R D, Driscoll M P. Pathological anatoing of dactylis glomerata infected by stagono sporarenaria. Phytopathology,1982,72:146-151.
    59. Jiang S J, Qiang S, Zhu YZ, Dong YF. Isolation and phytotoxicity of a,p-dehydrocurvularin, a metabolite from Curvularia eragrostidis and characterization of its modes of action. Annals of Applied Biology,2008,152:103-111.
    60. Johnson G A, Mortensen D A, Martin A R. A simulation of herbicide use based on weed spatial distribution. Weed Research,1995,35(3):197-205.
    61. Johnson D A, Baudoin A M. Mode of infection and factors affecting disease incidence of loose smut of crabgrass. Biological Control,1997,10:92-97.
    62. Johnstone G B, Bailey L B. Resistance to fungal penetration in Gramineae. Phytopathology,1985, 70:273-279.
    63. Kadir J B, Charudattan R and Berger R D. Effects of epidemiological factors on levels of disease caused by Dactyiaria higginsii on Cyperus rotundus. Weed Science.2000,48:61-68.
    64. Kelly N. Byer, Gary Peng, Thomas M. Wolf, Brian C. Caldwell, Spray retention and its eVect on weed control by mycoherbicides. Biological Control,2006,37:307-313.
    65. Kenney D S. Devine(?)-the way it development-an industrialist's view. Weed Science,1986,34: 15-16.
    66. Kim T J, Rossi F S, Neal J C. Inter-and intra-specific variation in crabgrass (Digitaria spp.). Weed Science Society of American Abstracts,1997,37:112.
    67. Kim J C, Choi G J, Heung T K. Pathogenicity and pyrenocine production of Curvularia inaequales straind from zoysia grass, Plant Disease,2000,84:684-688.
    68.. Kinane J and Oliver R P. Evidence that the appressorial development in barley powdery mildew is controlled by MAP kinase activity in conjunction with the cAMP pathway. Fungal Genetics and Biology,2003,39:94-102.
    69. King C A, Oliver L R. A model of predicting large crabgrass emergence as influcenced by temperature and water potential. Weed Science,1994,42:561-567.
    70. King K S, Gill G S. Studies on critcal period of weed competition in corn. Weed Science,1973, 5:1-6.
    71. Krause G H, Weis E. Chlorophyll fluorescence and photosynthesis:the basics. Annual Review of Plant Physiology and Plant Molecular Biology,1991,42:313-349.
    72. Kropp B R, Darrow H. The effect of surfactants and some herbicides on teliospore viability in Puccinia thlaspeos (Schub.). Crop Protection.2006,25:369-374.
    73. Krishna K, Singh R N, Kumar K. Leaf spot of barley caused by Curvularia lunata. Indian Phytopathology,2002,55:533.
    74. Lamm R D, Slaugter D C, Giles D K. Precision weed control system for cotton. Transactions of the ASAE,2002,45(1):231-238.
    75. Laurindo F R M, Pescatore L A, De Castro Fernandes D. Protein disulfide isomerase in redox cell signaling and homeostasis. Free Radical Biology and Medicine,2012,52:1954-1969.
    76. Li X, Wang C, Sun H and Li T. Establishment of the total RNA extraction system for lily bulbs with abundant polysaccharides. African Journal of Biotechnology,2011,10 (78):17907-17915.
    77. Li Y, Sun Z, Zhang X, Xu L, Chen Sand Li M. Reseach progress on microbial herbicides. Crop Protection,2003,22:247-252.
    78. Loretta Ortiz-Ribbing, Martin M. Williams, Potential of Phomopsis amaranthicola and Microsphaeropsis amaranthi, as bioherbicides for several weedy Amaranthus species. Crop Protection,2006,25:39-46.
    79. Lu DZ, Bai SE, Li XJ and Wang GQ. Study on the population ecology and control of Ascendent Crabgrass in summer grain crops in Hebei province. Acta Phytoecologica Sinica,1995,1:55-63.
    80. Lugo M, Talbert R E. Large crabgrass and smooth pigweed interference in snap bean[C]//Proceedings of the Annual Meeting-Arkansas Horticultural Society. Arkansas State Horticultural Society,1989:110.
    81. Makowski R D, Mortensen K. The first mycoherbicide in Canada:Colletotrichum gloeosporioides f. sp. malvae for round-leaf mallow control [A]. Richardson R G(ed.). Proceedings of the 2nd Internantional Weed Congress[C]. Melbourne:Weed Science Society of Victoria,1992,298-300.
    82. Makowski R M D. Effect of inoculum concentration, temperature, dew period, and plant growth stage on disease of round-leaved mallow and velvetleaf by Colletotrichum g loeosp orioid es f. sp. malvae. Phytopathology,1993,83:1229-1234.
    83. Malc E, Dzierzbicki P, Kaniak A, Skoneczna A and Ciesla Z. Inactivation of the 20S proteasome maturase, Umplp, leads to the instability of mtDNA in Saccharomyces cerevisiae. Mutation Research,2009,669:95-103.
    84. Martin M P, Junjper P E. Ultrastructure of lesions produced by Cercospora beticola in leaves of Beta Vulgaris. Plant Payhology,1979,15:13-26.
    85. Martin A, Lang D, Hanke S T, Mueller S J, Sarnighausen E, Vervliet-Scheebaum M and Reski R. Targeted gene knockouts reveal overlapping functions of the five Physcomitrella patens FtsZ isoforms in chloroplast division, chloroplast shaping, cell patterning, plant development, and gravity sensing. Molecular Plant,2009,6:1359-1372.
    86. Mena P J, Fernandez L L. New records of Cuvularia for sugarcane'(Saccharum sp. hybrid) in Cuba. Revista Iberoamericana de Micolagia,1993,10:2-4.
    87. Mohanbabu R, Sajeena A, Seetharaman k, Vidhyasekaran P, Rangasamy P, Som Prakash M, Som Prakash M, Senthil Raja A and Biji K R. Advances in bioherbicides development-an overiew. Crop Protection,2002,22(2):253-260.
    88. Monks D M, Schultheis J R. Critical weed-free period for large crabgrass in transplanted watermelon. Weed Science,1998,46:530-532.
    89. Monteiro F T, Vieira B S, Barreto R W. Curvularia lunata and Phyllachora spp.:two fungal pathogens of the grassy weed Hymenachne amplexicaulis from Brazil. Australasian Plant Pathology,2003,32:449-453.
    90. Mortenson K. The potential of an endemic fungus Colletotrichum gloeosporiodesf. sp. malvae for biological control of round-leaved mallow (Malva pusilla) and velvetleaf (Abutilon theophrastis). Weed Science,1988,36:473-478.
    91. Ondzighi C A, Christopher D A, Cho E J, Chang S C and Staehelin L A. Arabidopsis protein disulfide isomerase-5 inhibits cysteine proteases during trafficking to vacuoles before programmed cell death of the endothelium in developing seeds. The Plant Cell,2008,8: 2205-2220.
    92. Palmer W A, Heard T A, Sheppard A W. A review of Australian classical biological of weeds programs and research activities over the p ast 12 years. Biological Control,2010,52(7):271-287.
    93. Paula H, Michereff S J, Oliveira V S. Variability of Curvularia eragrostidis strains causing leaf blight of yam (Dioscorea cayennensis) in Pernambuco. Brasil. Boletin Micologico,2000, 15:85-92.
    94. Peng G, Boyetchko S M. Effect of variable dew temperatures on infection of green foxtail by Pyricularia setariae, Drechslera gigantea, and Exserohilum rostratum. Biological Control,2006, 39:539-546.
    95. Prom L K, Waniska R D, Kollo A I and Rooney W L. Response of eight sorghum cultivars inoculated with Fusarium thapsinum, Curvularia lunata, and a mixture of the two fungi. Crop Protection,2003,22:623-628.
    96. Qiang S. Zhu Y Z, Summerell B A, Li Y H. Mycelium of Alternaria alternata as a potential biological control agent for Eupatorium adenophorum. Biocontrol Science & Technology,2006, 16(7):653-668.
    97. Qiang S, Wang L, Wei R, Bing Zhou, Shiguo Chen, Yunzhi Zhu, Yunfa Dong, and Chuanfu An. Bioassay of the herbicidal actibity of AAC-Txiin produced by Alternaria alternate isolated from Ageratina adenophora. Weed Technology,2010,24:197-201.
    98. Quimby P C Jr, Fulgham F. E., Boyette C. D.,and Connick W J, Jr. An invert emulsion replaces dew in biocontrol of sicklepod Apreliminary study.1989,264-270 in:Pesticide Formulations and Application Systems. Vol.8, ASTM STP980. and Materials, Phila-delphia, PA.
    99. Rosado A, Schapire A L, Bressan R A, Harfouche A L, Hasegawa P M, Valpuesta V and Botella M A. The Arabidopsis tetratricopeptide repeat-containing protein TTL1 is required for osmotic stress responses and abscisic acid sensitivity. Plant physiology.2006,142:1113-1126.
    100. Rubiales D, Niks R E. Avoidance of rust infection by some genotypes of Hordeum chilense due to their relative inability to induce the formation of appressoria. Phsiological and Molecular Plant Pathology.1996,49;89-101.
    101. Ryals J A, Neuenschwander U H, Willits M G, Molina A, Steiner H Y, Hunt M D. Systenic acquired resistance. Plant Cell,1996,8:1809-1819.
    102. Saha A, Dasgupta S and Saha D. Discovery of Curvularia eragrostidis on tea (Camellia sinensis (L.)O. Ktze) leaves from clonal-cutting nurseries in North Bengal. Environment and Ecology, 2001,19:846-848.
    103. Sands D C, Ford E J and Miller R V. Genetic manipulation of broad host range fungi for biological control of weeds. Weed Technology,1990,4:471-474.
    104. Schnick P J, Stewart-Wade S M and Boland G J.2,4-D and Sclerotinia minor to control common dandelion. Weed Science,2002,50:173-178.
    105. Schroeder D, Mueller S H, Stinson C A S. European weed survey in 10 major crop systems to identify targets for biological control. Weed Research,1993,33:449-458.
    106. Seo P J, Park J M, Kang S K, Kim S G and Park C M. An Arabidopsis senescence-associated protein SAG29 regulates cell viability under high salinity. Planta,2011,233:189-200.
    107. Seo M, Peeters A J M, Koiwai H, Oritani T, Marion-Poll A, Zeevaart J A D, Koornneef M, Kamiya Y and Koshba T. The Arabidopsis aldehyde oxidase 3 (AAO3) gene product catalyzes the final step in abscisic acid biosynthesis in leaves. Proceedings of the National Academy of Sciences of the United States of America,2000,23:12908-12913.
    108. Shabana Y M. The use of oil emulsions for improving the efficacy of Alternaria eichhorniae as a mycoherbicide for waterhyacinth (Eichhornia crassipes). Biological Control,2005,32:78-89
    109. Shabana Y M, Stoever D M, Sauerborn J. Granular Pesta formulation of Fusarium oxysporum f. sp. orthoceras for biological control of sunflower broomrape:efficacy and shelf-life, Biological Control,2003,26:189-201.
    110. Shabana Y M, Charudattan R, James T D and Elwakil M A. An evaluation of hydrophilic polymers for formulating the bioherbicide agents Alternaria cassiae and A.eichhorniae. Weed Technology,1997,11:212-220.
    111. Shabana Y M. The use of oil emulsions for improving the efficacy of Alternaria eichhorniae as a mycoherbicide for waterhyacinth (Eichhornia crassipes). Biological Control,2005,32:78-89.
    112. Singh A K, Sinha R K P, Sinha R P. Cuvularia lunata:a major pathogen for leaf blight of wheat in Bihar, India. Journal of Applied Biology.1995,5:83-84.
    113.Singh R S. Some fruit rots of water melon in North India. Journal of Mycology and Plant Pathology,1984,14(3):279-280.
    114. Sisterna M N and Bello G M D. Curvularia protuberata, a new seed-borne pathogen of rice. Acta Phytopathologica et Entomologica Hungarica,1998,33:111-114.
    115. Smith D A, Hallett S G. Interactions between chemical herbicides and the candidate bioherbicide Microsphaeropsis amaranthi. Weed Science,2006:54(3):532-537.
    116.Srivastava A, Juttner F, Strasser R J. Action of the allelochemical, fischerellin A, on photosystem II. Biochimica et Biophysica Acta,1998,1364:326-336.
    117. St Leger R J and Screen S. Genetic improvement of fungi for insect and weed control. In Butt TM, Jackson C and Morgan N (ed.), Fungal biocontrol agents:progress, problems and potential. CABI, Wallingford, United Kingdom.2002.
    118.Strasser R J, Srivastava A, Tsimilli-Michael M. The fluorescence transient as a tool to characterize and screen photosynthetic samples [M]. In Probing Photosynthesis:Mechanism, Regulation and Adaptation. London:Taylor and Francis,2000,445-483.
    119.Strasser R J, Tsimilli-Michael M, Srivastava A. Analysis of the chlorophyll a fluorescence transient, Chlorophyll Fluorescence:A Signature of Photosynthesis [M]. Netherlands:Kluwer Academic Publishers Press,2004,1-45, Chap.12.
    110. Strasser R J, Srivastava A and Govindjee. Polyphasic chlorophylla fluorescence transient in plants and cyanobacteria, Photochemistry and Photobiology,1995,61:32-42.
    111. TeBeest D O and Templeton G E. Mycoherbicides progress in the biological control of weeds. Plant Disease,1985,69 (1):6-10.
    112. Templeton G E. Status of weed control with plant pathogens, in Charudattan R. and Walker H L. Biological control of weeds with plant pathogens. New York Wiley,1982,29-35.
    113. Tilley A M, Walker H L. Evaluation of Curvularia intermedia (Cochliobolus intermedius) as a potential microbial herbicide for large crabgrass (Digitaria sanguinalis). Biological Control,2002, 25:12-21.
    114. Voigt C A, Schafer W, Salomon S. A secreted lipase of Fusarium graminearum is a virulence factor required for infection of cereals, Plant Journal,2005,42(3):364-375.
    115.Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hoenes M, Friters A, Pot J, Paleman J, Kuiper M and Zabeau M. AFLP:a new technique for DNA fingerprinting. Nucleic Acids Research, 1995,23:4407-4414.
    116. Vurro M, Zonno M C, Evidente A, Andolfi A and Montemurro P. Enhancement of efficacy of Ascochyta caulina to control Chenopodium albumn by use of phytotoxins and reduced rates of herbicides. Biological Control,2001,21 (2):182-190.
    117. Walker H L and Tilley A M. Control of crabgrass with a fungal pathogen. United States Patent: 5952264,1997.
    118. Walker H L. Alternaria macrospora as a potential biolocontrol agent for spurred anoda: production of spores for field studies.U.S.Dep.Agrc.Adv. Agric. Technology South Service. (ISSN 0193-3728) No.12.1980,5.
    119. Walke H L. Granular formulations of Alternaria macrospora for control of spurred anoda(Anoda cristata). Weed Science,1981,29:342-245.
    120. Walker R H, Wehtje G and Richburg J S. Interference and control of large crabgrass (Digitaria sanguinalis) and southern sandbur(Cenchrus echinatus) in forage bermudagrass(Cynodon dactylon). Weed Technology,1998,12:707-711.
    121. Wang F, Peng Z, Qiang S, Xu L. Interaction of plant epicuticular waxes and extracellular esterases of Curvularia eragrostidis during infection of Digitaria sanguinalis and Festuca arundinacea by the fungus. International Journal of Molecular Sciences,2006,7 (9):346-357.
    122. Wang F, Peng Z, Qiang S, Zhu YZ and Xu LL. Effects of epicuticular wax from Digitaria sanguinalis and Festuca arundinacea on infection by Curvularia eragrostidis. Australasian Plant Pathology,2008,37,43-52.
    123. Wang JS, Wang XM, Yuan BH, Qiang S. Differential gene expression for Curvularia eragrostidis pathogenic incidence in crabgrass(Digitaria sanguinalis) revealed by cDNA-AFLP analysis. Plos One,2013,8(10):e75430
    124. Watson A K. Current advances in bioherbicide research. In:Proceedings of the Brighton Crop Protection Conference. Weeds. UK.1989,987-996.
    125. Webster T M, MacDonald G E. A Survey of Weeds in Various Crops in Georgia. Weed Technology,2001,15(4):771-790.
    126. Weston L A. Cover crops and herbicide influence on row crop seeds establishment in no-tillage culture. Weed Science,1990,38:166-171.
    127. Weston L A. Utilization of allelopathy weed management in agroecosystens. Agronomy Journal, 1996,88:860-866.
    128. Wezel A. Weed vegetation and land use of upland maize fields in north-west Vietnam. GeoJournal,2000,50(4):349-357.
    129. Wiederholt R J, Stoltenberg D E. Large crabgrass [Digitaria sanguinalis (L.) Scop.] resistance to several acetyl-CoA carboxylase inhibitors. The Proceedings of North Central Weed Science Society. 1993,48:20.
    130. WiederholtI R J, Stoltenberg D E. Cross-resistance of a large crabgrass (Digitaria sanguinalis) accessions to aryloxyphenoxypropionate and cyclohexanedione herbicides. Weed Technology,1995, 9:518-524.
    131. Wiederholt R. J, Stoltenberg, D. E. Similar fitness between large crabgrass (Digitaria sanguinalis) accessions resistant or susceptible to acetyl-coenzyme A carboxylase inhibitors. Weed Technology,1996,10 (1):42-49.
    132. Winder R S. and Van Dyke C G. The pathogenecity, virulene, and biocontrol potential of two Bipolaris species of johnsongrass(Sorghum halepense). Weed Science,1990,38:89-94.
    133. Wymore L A, Watson A K. and Gotlieb A R. Ineraction between Colletotrichum coccodes and thidiazuron for control of velvetleaf (Abutilon theophrasti). Weed Science,1987,35:377-383.
    134. Xueyan Li, Chunxia Wang, Hongmei Sun, Tianlai Li. Establishment of the total RNA extraction system for lily bulbs with abundant polysaccharides. African Journal of Biotechnology,2011,10 (78):17907-17915.
    135. Yesbergenova Z, Yang G H, Oron E, Soffer D, Fluhr R and Sagi M. The plant Mo-hydroxylases aldehyde oxidase and xanthine dehydrogenase have distinct reactive oxygen species signatures and are induced by drought and abscisic acid. Plant Journal,2005,6:862-876.
    136. Zahran E, Sauerborn J, Abbasher A A, Ahmed E A, Mohukker R I, Karlovsky P, Mohamed E Aand Miiller-Stover D.'Pesta" and alginate delivery systems of Fusarium spp. for biological control of Striga hermonthica (Del.) Benth. under Sudanese field conditions. Biological Control, 2008,44:160-168.
    137. Zhang W, Wolf T M, Bailey K L, Mortensen K and Boyetchko S M. Screening of Adjuvants for bioherbicide formulations with Colletotrichum spp. and Phoma spp. Biologycal Control,2003, 26:95-108.
    138. Zhang W and Waston A K. Characterization of growth and conidia production of Exserohium monceras on different substrates. Biocontrol Sciences and Technology,1997,7:75-861.
    139. Zimdahl R L. Fundamentals of weed science [M].2nd ed. San Diego. California:Academic Press,1999,247-269.
    140. Zhu Y, Qiang S. Isolation, Pathogenicity and Safety of Curvularia eragrostidis Isolate QZ-2000 as a Bioherbicide Agent for Large Crabgrass (Digitaria sanguinalis). Biocontrol Science and Technology,2004,14(8):769-782.
    141.暴增海,马桂珍,杨文兰等.玉米弯孢霉叶斑病的初侵染来源及几种杀菌剂的室内毒力测定[J].吉林农业大学学报,2002,24(4):53-57
    142.卞彦,谈建康,张继林.钠盐胁迫对小麦叶片中过氧化氢和核酸含量的影响[J].南京林业大学学报,2000,3(24):65-67.
    143.曾任森,林象联,骆世明,曾强,谭惠芬.蟛蜞菊的生化他感作用及生化他感作用物的分离鉴定[J].生态学报,1996,1:20-27.
    144.陈乾锦,陈家骅,张玉珍等.烟田杂草的综合防除[J].植物医生,2001,14(1):27-28..
    145.陈勇,倪汉文.尖角突脐孢对稻田稗草的防除效果[J].植物保护学报,2001,28(1):73-76.
    146.陈勇强,但汉斌,郭富常.国外微生物除草剂的研究及应用现状[J].天津农业科学,1998,4(2):5-9
    147.陈燕芳,丁伟,丁吉林,郭文明,尚德斌.天然产物除草剂研究进展[J].杂草科学,2007(2):1-5.
    158.程亮,朱海霞,郭青云.3株镰刀菌的鉴定、对野燕麦的致病力及其对5种作物的影响[J].中国生物防治,2010,26(S1):84-89.
    149.程曦,田彩娟,李爱宁,邱金龙.植物与病原微生物互作分子基础的研究进展[J].遗传,2012,34(2):134-144.
    150.崔延堂,李智选,任毅等.冷季型草坪杂草的季节性发生与演替[J].生态学杂志,2003,22(2):20-23.
    151.邓功成,刘朝贵.不同培养基对鸡腿蘑菌丝体同工酶影响的研究[J].西南农业大学学报(自然科学版),2004,1:55-57.
    152.丁国昌,林思祖,曹光球等.渗透胁迫下邻羟基苯甲酸对1年生杉木幼苗叶绿素含量的影响[J].西南林学院学报,2008,28(1):39-42.
    153.杜金河,吕述斗.淮北夏大豆田杂草发生及化除技术研究[J].农药,2003,42(2):35-37.
    154.付颖,叶非.生物源除草剂研究与使用进展[J].农药,2002,41(5):7-11.
    155.高昭远,干静娥.菟丝子的生物防除—“鲁保一号”的研究进展[J].生物防治通报,1992,8(4):173-175.
    156.高同军.生物除草剂敌散克(Disancu)工业化生产、剂型、配方及田间控草技术研究.硕十学位论文南京农业大学.2006
    157.高柱平,李孙荣.夏花生田马唐的生态经济阈值、生态经济除草阈值模型的研究[J].植物保护学报,1989,2:139-143
    158.耿锐梅,曹长代,董世峰,冯全福,陈志强,蒋彩虹;胡海州;张玉;罗成刚.植物寄主与病原真菌互作研究进展[J].中国果菜,2011,4:23-26.
    159.郭凤根,李扬汉.园林菟丝子生防真菌喷施条件的研究[J].中国生物防治,2000,16(2):81-83.
    160.古燕翔,王代军.外源诱导物水杨酸对草坪型高羊茅弯孢霉叶斑病抗性影响的研究[J].中国草地,2003,25(4):56-61.
    161.郝建华,强胜.被子植物有性和无融合生殖过程中胼胝质壁的动态变化及其生理学意义[J].植物生理学通讯,2006,42:141-147.
    162.胡萃,王韧.国外应用微生物防治杂草的进展,生物防治通报[J],1987,3(1):35-38.
    163.贺春萍,付相芹,张荣意等.西瓜弯孢霉叶枯病的初步研究[J].热带农业科学,2000,(1):14-20.
    164.胡萃,王韧.国外应用微生物防治杂草的进展,生物防治通报[J],1987,3(1):35-38.
    165.韩烈刚,韩烈保,刘荣堂等.几种除草剂对马唐和稗草的灭效[J].北京林业大学学报,2000,22(2):9-11.
    166.何晨阳,王金生.抗病植物的防卫反应和机制[J].生物学通报,1994,5:9-10.
    167.胡树香,张福远,王琳,黄开玉.夏作大豆田杂草一次性防除技术研究[J].山东农业科学,2000,3:37-38.
    168.黄春艳.利用植物病原真菌防除杂草研究进展.黑龙江农业科学[J],2000,3:43-44.
    169.黄世文,余柳青.淡紫灰吸水链霉菌及其紫外诱变菌株用于害物生防研究[J].农业生物技术学报,2000,8(1):79-84
    170.黄世文,余柳青,Watson A K.影响链格孢菌生长及产孢的因子[J].中国生物治,2001,17(17):16-19.
    171.黄世文,余柳青,Watson A K.影响链格孢菌生长及产孢的因子[J].中国生物防治,2001,17(1):10-151.
    172.黄世文,余柳青,段桂芳等.一种筛选稗草生防潜力菌的简易生测方法[J].中国生物防治,2004,20(1):53-56.
    173.黄思良Alternaria alternata菌群的菌丝融合及附着胞形成的研究[J].植物病理学报,1998,1:67.
    174.姜德锋,李孙荣,由振国.北京地区夏玉米田马唐治理的生态经济阈值研究[J].莱阳农学院学报,1996,3:44-47.
    175.姜德锋,陈洁敏,林文彬等.玉米田杂草马唐的生长特性研究[J].莱阳农学院学报,2000,17(2):113-115.
    176.姜述君,强胜,朱云枝.画眉草弯孢霉菌除草剂活性化合物的分离鉴定及其生物活性测定[J].植物保护学报,2006,33(3):313-318.
    177.姜述君.画眉草弯孢霉菌主要致病毒素的鉴定及其致病机理和除草剂潜力的研究.博士学位论文南京农业大学.2005
    178.姜述君,杨云强,石园园,范文艳,戴凌燕.生防菌Alteria amarnthi-3对反枝苋的防治效果[J].植物保护学报,2010,39(1):78-82.
    179.姜述君,强胜.马唐生防菌画眉草弯孢霉毒素α,β-dehydrocurvularin对马唐叶绿体功能的影响[J].植物病理学报,2005,35(4):312-216.
    180.姜述君,强胜.马唐生防菌画眉草弯孢霉毒素α,β-dehydrocurvularin对马唐叶片PSⅡ的影响[J].中国农业学,2005,38(7):1373-1378.
    181.金爱武,胡综超,张卓文.雷竹鞭侧芽发育过程中核酸含量、过氧化物酶和淀粉酶同工酶的变化[J].竹子研究汇刊,1990,1(15):15-20.
    182.孔琼,杨红玉,王云月,霍达,胡海林,刘振华,秦丽娟.植物与病原物互作中的细胞成序化死亡[J].植物保护,2012,38(6):1-6.
    183.李铷,董锦艳,向梅梅.微生物源除草剂的研究、应用现状及展望[J].杂草科学,2004,4:1-7
    184.李香菊,褚建君,李扬汉等.农达与乙阿合剂桶混防除免耕夏玉米田杂草[J].江苏农业科学,2000,(2):46-49.
    185.李香菊,王贵启,王勤等.农达与乙阿合剂桶混防除免耕玉米田杂草试验报告[J].河北师范大学学报(自然科学版),2001,25(2):239-242,250.
    186.李香菊,王贵启,段美生等.免耕夏玉米田马唐的生物学特性与治理措施[J].河北农业科学,2003,7(1):16-21.
    187.李扬汉,张宗俭,王建书,冀玲慧.有关真菌除草剂研究的进展[J].生物防治通报,1994,10(1):35-39.
    188.刘焕禄,刘亦学,刘晓琳,张学文,赵士敏.微生物除草剂研究概况与建议[J].天津农学院学报[J],2000,7(4):36-39.
    189.刘志海,朱全让.鲁保一号菌[M].山东科技出版社,1980.
    190.刘俊松,张上隆.柑橘花芽分化期结果和未结果树核酸含量变化[J].西南大学学报(自然科学版),2008,6(30):56-59.
    191.刘世鹏,刘济明,曹娟云,陈宗礼.水分胁迫对枣苗抗氧化物酶活性及其核酸含量的影响[J].安徽农业科学,2006,34(8):1525-1527.
    192.卢向阳,吴罗罗,李孙荣,李秉礼,李辉.阿特拉津与丁草胺混用防除夏玉米田杂草的研究[J].农药科学与管理,1992,1:29-33.
    193.路艳娜.复合生物除草剂敌散克(Disanca)的剂型研究.硕十学位论文南京农业大学.2010
    194.吕德滋,白素娥,李香菊,王贵启.升马唐种群生态及其田间密度调控指标的研究[J].植物生态学报,1995,1:55-63.
    195.强胜.链格孢菌作为真菌除草剂防除紫茎泽兰潜力的研究[D].南京农业大学博十学位论文.1998.
    196.强胜.杂草科学面向生物科学时代的机遇与挑战(上)[J].世界农业,2001,4:37-39
    197.强胜.杂草科学面向生物科学时代的机遇与挑战(下)[J].世界农业,2001,5:42-43
    198.强胜主编,杂草学[M].北京:中国农业出版社,2001:2.
    199.强胜,陈世国.生物除草剂研发现状及其面临的机遇与挑战[J].杂草科学,2011,29(1):1-6.
    200.强胜,郭爱民,李扬汉.大批量生产百日草链格孢菌孢子的技术[J]中国生物防治,1997,13(4):169-1721
    201.强胜,胡金良.江苏省棉区棉田杂草群落发生分布规律的数量分析[J].生态学报,1999,19(6):810-816.
    202.强胜,李广英.南京市草坪夏季杂草分布特点及防除措施研究[J].草业学报,2000,9(1):48-54.
    203.强胜,宋小玲,戴为民.抗除草剂转基因作物面临的机遇与挑战及其发展策略[J].农业生物技术学报,2010,18(1):114-125.
    204.邱诞根,涂依琴,朱金星等.禾耐斯防除甘薯地杂草的效果[J].农药,2001,40(8):34.
    205.沈萍.微生物学[M].北京:高等教育出版社,2001,182-861.
    206.石洁,刘玉瑛,魏利民等.玉米弯孢菌叶斑病病原菌的研究[J].沈阳农业大学学报,2000,31(5):479-481.
    207.申继忠.微生物农药剂型加工研究进展[J].中国生物防治,1998,3:34-38.
    208.苏少泉.生物除草剂的研究与开发[J].农药,2004,43(3):97-100.
    209.苏微微.广西龙眼、荔枝、香蕉园杂草调查初报[J].柑桔与亚热带果树信息,2001,17(5):45-46.
    210.唐洪元,王学鹗,喻如俊,陶妍.马唐、千金子对棉花的危害[J].上海农业科技,1987,2:20-22.
    211.唐洪元,王学鹗,胡亚琴.杂草种子寿命的研究[J].植物生态学与地植物学学报,1988,1:72-78.
    212唐洪元,王学鹗,杨姿.中国棉田杂草的分布危害及防除[J].上海农业学报,1989,3:51-58.
    213.王飞.画眉草弯孢霉菌特异性入侵马唐中病原菌酯酶和植物叶表层蜡质及其相互作用.博士学位论文南京农业大学.2006
    214.王怀玉,罗英.基质对VA菌根真菌的侵染及孢子产量的影响[J].四川师范大学学报(自然科学版),2003,26(3):302-305.
    215.王建书,朱云枝,刘贵巧,强胜.画眉草弯孢霉菌株QZ-2000及其靶标若干酶活性测定[J].河南农业科学,2010,1:73-79
    .216.王建书,朱云枝,赵敏,强胜.画眉草弯孢霉菌株QZ-2000与低量化学除草剂复配增效机制[J].河南农业科学,2010,1:65-70
    217.王世梅,黄为一,武济民.链霉菌NND—52菌株及其次生代谢物的鉴定[J].南京农业大学学报,1994,4:54-59.
    218.韦韬,李静,倪汉文.稗草生防菌新月弯孢菌株J15(2)的生物学特性[J].中国生物防治,2009,25(1):54-59.
    219.吴保峰,刁治民,熊亚.微生物除草剂的研究现状及应用前景[J].青海草业,2004,13(2):34-39.
    220.吴宪伟.大豆田除草剂混用的除草效果[J].杂草科学,1995,1:30-31.
    221.向梅梅,曾永三,刘任,陈圣情,蔡军宏,游明龙.莲子草假隔链格孢的寄主范围及对空心莲子草的控制作用[J].植物病理学报,2002,32(3):286-287.
    222.徐春凤.生物除草剂敌散克(Disanke)产业化若干关键技术的研究.硕士论文.南京农业大学2004
    223.徐志德,刘岗山,黄河清等.湖南省甘蔗病虫草害调查报告[J].湖南农业科学,2000,1:26-27.
    224.曾青,强胜.波斯婆婆纳生防菌株QZ-97a的分离鉴定与致病性研究[J].南京农业大学学报,2000,23(3):21-24.
    225.张猛,张天宇.中国弯孢属分类研究Ⅰ.生于禾本科植物上的一个新种和一个中国新记录种[J].菌物学报,2004,23(3):328-330.
    226.张玉聚,宋凤仙,孔建,尚嘉彦,王恒亮,肖敬德.50%禾宝乳油防除大豆与花生田杂草的研究[J].杂草科学,1998,2:25-27.
    227.张宗俭,李扬汉.野燕麦生防真菌燕麦叶枯菌的分离培养及致病性研究[J].中国生物防治,1996,12(4):171-173.
    228.朱海波,赵桂东,周玉梅,李茹,姚东瑞.夏大豆田杂草消长规律及化除技术研究[J].杂草科学,1998,1:38-39.
    229.朱海霞,程亮,郭青云.野燕麦生防菌株HZ-31的分离与致病性研究[J].西北农业学报,2012,21(6):167-173.
    230.朱克明,沈晓昆,谢桐洲.稻鸭共作技术试验初报[J].安徽农业科学,2001,29(2):262-264.
    231.朱云枝,强胜.马唐生防真菌弯孢霉(Curvularia sp.)菌株QZ-200的生物学特性研究[J].植物病理学报,2003,33(6):524-529.
    232.朱云枝,强胜.马唐病原真菌的分离筛选及其致病力测定[J].中国生物防治,2004,20(3):206-210.
    233.朱云枝,强胜.真菌菌株QZ-200对马唐(Digitaria sanguinalis)致病力的影响因子[J].南京农业大学学报,2004,27(2):47-50.
    234.朱云枝,强胜.马唐生防真菌弯孢霉(Curvularia sp.)菌株QZ-200的生物学特性研究[J].南京农业大学学报,2004,27(2):47-50.
    235.周兵,闫晓红,钟娟,蒋平,强胜.链格胞菌毒素细交链格胞酮酸对空心莲子草的致病性[J].浙江农业学报,2010,22(2):215-219.
    236.杨好伟,姚秋菊,刘学芝.干旱胁迫对不同生育时期冬小麦叶片蛋白质及核酸含量的影响[J].河南农业大学学报,2001,35:1-3.
    237.杨瑞先,范晓静,邱思鑫,蔡学清,胡方平.利用cDNA-AFLP内生解淀粉芽孢杆菌Fyll诱导辣椒差异表达基因[J].中国农业科学,2013,46(12):2449-2458.

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