复方黄芪多糖注射剂的安全性和增强仔猪免疫效果的研究
摘要
选取小白鼠50只随机分为5组进行复方黄芪多糖注射液安全性试验;选取家兔2只分为二组进行局部刺激性试验;选取42日龄断奶仔猪90头随机分为3组,注射不同剂量复方黄芪多糖注射液,观察其对血液指标的影响。选用家兔6只随机分为3组进行家兔体温试验和溶血试验;选用20头21日龄仔猪,随机分为4组进行猪瘟阻断试验;选用1日龄杜×长×大三元哺乳仔猪150头共15窝随机为3组进行“新三针保健”,观察其对仔猪生长性能的影响。结果表明:复方黄芪多糖注射液对小白鼠的LD_(50)为0.95mL;家兔局部注射药物后吸收良好,刺激性低;猪颈部肌内注射3mL/头、6mL/头药物后,血液中RBC、PLT均有一定程度提高,差异显著(P＜0.05),HCT也有一定程度升高,但差异不显著(P＞0.05),而WBC,HGB则都有一定程度下降,但差异不显著(P＞0.05),WBC含量比较高,可能与存在少量炎症有关,血液中总蛋白和白蛋白适当提高,而白球比(A/G)在正常范围之内,与未给药前相比差异不显著(P＞0.05),与对照组相比各项指标差异不显著(P＞0.05),说明复方黄苠多糖注射液几乎无毒,刺激性较小,在3-6mL/头剂量范围对血液生化指标没有明显影响,且对血液生理指标有明显改善作用。家兔体温试验结果表明,试验组与阳性对照组兔体体温变化均大于1.5℃,差异不显著(P＞0.05),并且呈现高稽留热型;在溶血试验中其溶血率＜0.5%,显微观察兔红细胞无溶血破裂现象。说明复方黄芪多糖注射液没有直接杀灭猪瘟疫苗作用,也不会引起溶血。免疫增效试验结果表明,三个试验组猪瘟抗体水平均有较大幅度提高,其阳性率达100%,而对照组抗体水平相对较低。“新三针保健”优于传统三针保健组,其腹泻率低16%,其它发病率也低20%,其仔猪多增重12.6%,但差异不显著(P＞0.05),与对照组差异显著(P＜0.05),能明显增加仔猪血清中免疫球蛋白IgG、IgA的含量,差异显著(P＞0.05)。
In order to study the innocuity tests of compound astragalus polysaccharide injection, 50 Mus musmus-culus were randomly divided into 5 groups. Selected two rabbits into two groups for local irritant test, 90 weaned pigs at 42 days randomly divided into three groups, were injected different dose compound astragalus polysaccharide injection, and were observed blood index of the injection. 6 rabbits were randomized into three groups to study temperature test and hemolytic test, Selected 20 piglets at 21 days, randomly divided into four groups for swine pest blocking test, 150 suckled pigs at 1 day for 15 nests were randomly divided into 3 groups for new three needles care, and to be observed its health effects of pigs' growth performance. The results showed that: the LD50 of the compound astragalus polysaccharide injection for musmus-culus was 0.95mL. After the local injections in rabbits, the stimulating was in a low, was absorbed well. After intramuscular injection at neck for 3mL, 6mL per pig, RBC and PLT in blood were improved in a certain extent, the difference was obvious (P<0.05), and HCT also had increased in certain degree, but there was no significant difference (P>0.05), and the WBC and HGB had been lower in certain degree, but no significant difference (P>0.05), the WBC was higher relatively, it's possible which had some inflammation, the total protein and albumin in blood increased appropriately, the proportionality of albumin and globulin (A/G) were in the normal range, and there was no significant difference before no giving medicine (P>0.05), compared with the control group, all kinds of indexes in blood had no significant difference (P> 0.05). Compound astragalus polysaccharide injection was almost a non-toxic, nonirritant injection; the dose of 3-6mL per pig had no obvious effects on blood biochemical indicators, and a clear improvement on blood physiological indexes. The rabbits body temperature tests showed that, the control group with positive enrichment temperature changes were greater than 1.5 degrees, there is no significant difference (P>0:05), and has high heat; Its hemolytic test showed that the hemolytic rates were lower than 0.5%, rabbit RBC's microexamination was observed had no hemolysis. Showed that it could not kill directly vaccine virus, and it didn't cause hemolysis. The immunopotentiation experimental results showed that, three experimental groups had an antibody levels greatly improved, and it were 100%, and the control group was compared with relatively low level of antibodies. The new three needles health care groups were better than traditional three needles care group, the incidence rate of diarrhea was lower 16%, other illnesses were lower 20%, there were more than 12.6% weight gain, but no significant differences (P> 0.05), and it's difference significant compared with the control group (P<0.05), it could obviously increase the pigs serum levels of immunoglobulin IgG and IgA, and difference significant (P<0.05).
In order to study the innocuity tests of compound astragalus polysaccharide injection, 50 Mus musmus-culus were randomly divided into 5 groups. Selected two rabbits into two groups for local irritant test, 90 weaned pigs at 42 days randomly divided into three groups, were injected different dose compound astragalus polysaccharide injection, and were observed blood index of the injection. 6 rabbits were randomized into three groups to study temperature test and hemolytic test, Selected 20 piglets at 21 days, randomly divided into four groups for swine pest blocking test, 150 suckled pigs at 1 day for 15 nests were randomly divided into 3 groups for new three needles care, and to be observed its health effects of pigs' growth performance. The results showed that: the LD50 of the compound astragalus polysaccharide injection for musmus-culus was 0.95mL. After the local injections in rabbits, the stimulating was in a low, was absorbed well. After intramuscular injection at neck for 3mL, 6mL per pig, RBC and PLT in blood were improved in a certain extent, the difference was obvious (P<0.05), and HCT also had increased in certain degree, but there was no significant difference (P>0.05), and the WBC and HGB had been lower in certain degree, but no significant difference (P>0.05), the WBC was higher relatively, it's possible which had some inflammation, the total protein and albumin in blood increased appropriately, the proportionality of albumin and globulin (A/G) were in the normal range, and there was no significant difference before no giving medicine (P>0.05), compared with the control group, all kinds of indexes in blood had no significant difference (P> 0.05). Compound astragalus polysaccharide injection was almost a non-toxic, nonirritant injection; the dose of 3-6mL per pig had no obvious effects on blood biochemical indicators, and a clear improvement on blood physiological indexes. The rabbits body temperature tests showed that, the control group with positive enrichment temperature changes were greater than 1.5 degrees, there is no significant difference (P>0:05), and has high heat; Its hemolytic test showed that the hemolytic rates were lower than 0.5%, rabbit RBC's microexamination was observed had no hemolysis. Showed that it could not kill directly vaccine virus, and it didn't cause hemolysis. The immunopotentiation experimental results showed that, three experimental groups had an antibody levels greatly improved, and it were 100%, and the control group was compared with relatively low level of antibodies. The new three needles health care groups were better than traditional three needles care group, the incidence rate of diarrhea was lower 16%, other illnesses were lower 20%, there were more than 12.6% weight gain, but no significant differences (P> 0.05), and it's difference significant compared with the control group (P<0.05), it could obviously increase the pigs serum levels of immunoglobulin IgG and IgA, and difference significant (P<0.05).
引文
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