OX40L及其基因多态性与冠心病严重程度的关系
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摘要
目的
探讨不同类型冠心病患者的血清OX40配体(OX40L)和C反应蛋白(C reactive protein,CRP)的水平及其临床意义。
方法
选取行选择性冠状动脉造影术检查者共458例作为研究对象,根据临床表现及冠状动脉造影的结果分为三组,其中急性冠脉综合征(ACS)177例,稳定性心绞痛(SAP)89例,非特异性胸痛者192例。分别采用ELISA法和免疫透射比浊法测定各组患者的血清OX40L和CRP的浓度,采用方差分析比较不同组患者血清OX40L和CRP的水平;采用直线相关分析分析OX40L与CRP的关系。
结果
(1)急性冠脉综合征(ACS)组OX40L和CRP的水平明显高于稳定型心绞痛(SAP)组和正常对照(CT)组(P<0.01)。
(2)血清OX40L的浓度与CRP的浓度呈正相关(r=0.780, P﹤0.01)。
结论
血清OX40L和CRP的水平与冠心病患者的病情严重程度相关密切,OX40L与CRP一样有可能作为炎症指标,预测冠心病的发生及其预后。
目的
探讨血清OX40配体蛋白(OX40L)的水平与冠状动脉病变程度之间的相关性。
方法
选取行选择性冠状动脉造影术检查者共458例作为研究对象,根据冠状动脉造影结果冠状动脉病变的支数,分为1支病变组、2支病变组、3支病变组和冠脉造影正常的对照组(0支病变组),冠状动脉病变程度由冠状动脉病变支数和Gensini积分表示,采用ELISA法测定患者的血清OX40L的浓度,对OX40L水平与冠脉病变程度进行单因素和多因素分析。
结果
(1)冠状动脉病变各组OX40L的水平显著高于正常对照组(P<0.05或P<0.01),血清OX40L的水平与冠状动脉病变程度和Gensini积分呈正相关(P<0.01)。
(2)多元逐步回归分析结果显示在校正了年龄、高血压和高密度脂蛋白胆固醇(HDL-C)后,血清OX40L的水平与冠状动脉病变总积分(r=4.763,P 结论
冠心病(CHD)患者的血浆OX40L的水平与冠状动脉病变程度密切相关,可能对冠心病有一定的预测和诊断意义。
目的
研究中国重庆地区汉族人群OX40配体蛋白基因(TNFSF4)的单核苷酸多态性在人群中的分布情况,并探讨该多态性与冠心病之间的关系。
方法
对453例患者进行选择性冠状动脉造影术,根据冠状动脉造影结果和临床资料分为二组:冠心病组262例和正常对照组191例,提取各组患者的全血基因组DNA,采用Taqman实时荧光定量PCR法检测TNFSF4 rs3850641(A/G)的单核苷酸多态性。
结果
(1)重庆地区汉族人群中存在TNFSF4 rs3850641(A/G)的单核苷酸多态性,在冠心病组中基因型分布为:AA型70.5%,AG型22.0%、GG型7.5%;在对照组基因型分布为:AA型69.8%,AG型20.8%、GG型9.4%。
(2)冠心病组和对照组之间的基因型频率及等位基因频率均无显著性差异(P>0.05)。
结论
OX40L基因rs3850641(A/G)的单核苷酸多态性与中国重庆地区汉族人群冠心病发病可能无关。
Objective
To study the Clinical significance of the level of serum OX40 ligand (OX40L) and C reactive protein(CRP) in patients with coronary atherosclerotic heart disease(CHD).
Methods
The level of OX40L was measured by enzyme-linked immunosorbent assay(ELISA) in patients with stable angina pectoris(SAP), acute coronary syndromes(ACS),and normal control group. The level of CRP was measured by immunoturbidimetric method,and the severity of coronary artery lesion was evaluated according to coronary angiography.
Results
(1)The levels of OX40L and CRP were significantly higher in acute coronary syndromes(ACS) group than those in stable angina pectoris(SAP) and control groups(P<0.01).
(2)The level of OX40L was positively associated with the level of CRP(r=0.780, P﹤0.01).
Conclusions
The levels of OX40 ligand and CRP are related with the CHD’severity.Serum OX40L and CRP were sensitive markers of inflammation and were helpful for assessment of the severity of disease and outcome prediction in patients with CHD.
Objective
To investigate the correlation of serum OX40 ligand level and severity of coronary lesion in coronary atherosclerotic heart disease (CHD) patients.
Methods
458 patients underwent coronary angiography were divided into 4 groups according to the number of affected blood vessels: normal control group, single vessel damage group, double and three vessels damage groups. The severity of coronary lesion was claasified by the number of affected blood vessels and the CHD Gensini cumulative index. Their level of OX40L was measured by enzyme-linked immunosorbent assay(ELISA).Both univariate and multivariate analyses were employed.
Results
(1)The OX40L serum levels of the CHD patients were significantly higher than those in control group (P<0.01～0.05). Univariate analysis revealed the number of affected vessels was increased along with the increasing OX40L serum level. OX40L levels were positively correlated with the severity of coronary lesion and the Gensini index (P<0.01).
(2) After adjusting co-variations such as ages,hypertension and plasma HDL-C levels,OX40L levels still showed correlation with the Gensini index significantly (r=4.763,P<0.01).
Conclusions
The level of OX40L had association with the severity of angiographic coronary artery disease,which may contribute to the early diagnosis of CHD and the disease prognostic indication.
Objective
To investigate the genotype distribution of TNFSF4 gene polymorphism and to study the relationship between TNFSF4 rs3850641(A/G) gene polymorphisms and coronary heart disease(CHD).
Methods
453 subjects were include:262 coronary heart disease patients(CHD group),191 normal subjects(normal control group).All subjects were confirmed by coronary angiography,and were genotyped for the gene polymorphism of TNFSF4 rs3850641(A/G) by TaqMan real-time PCR.
Results
(1)Han nationality population in Chongqing had TNFSF4 rs3850641(A/G) gene polymorphisms,the frequencies of AA,AG and GG genotypes were 70.5% ,22.0% and 7.5% in CHD group while ones were 69.8%, 20.8% and 9.4% in controls.
(2)There were no significant differences in frequencies of genotype and allele between controls and CHD cases(P>0.05).
Conclusions
TNFSF4 rs3850641(A/G) gene polymorphisms should be independent of occurrence of CHD in Han nationality population in Chongqing.
探讨不同类型冠心病患者的血清OX40配体(OX40L)和C反应蛋白(C reactive protein,CRP)的水平及其临床意义。
方法
选取行选择性冠状动脉造影术检查者共458例作为研究对象,根据临床表现及冠状动脉造影的结果分为三组,其中急性冠脉综合征(ACS)177例,稳定性心绞痛(SAP)89例,非特异性胸痛者192例。分别采用ELISA法和免疫透射比浊法测定各组患者的血清OX40L和CRP的浓度,采用方差分析比较不同组患者血清OX40L和CRP的水平;采用直线相关分析分析OX40L与CRP的关系。
结果
(1)急性冠脉综合征(ACS)组OX40L和CRP的水平明显高于稳定型心绞痛(SAP)组和正常对照(CT)组(P<0.01)。
(2)血清OX40L的浓度与CRP的浓度呈正相关(r=0.780, P﹤0.01)。
结论
血清OX40L和CRP的水平与冠心病患者的病情严重程度相关密切,OX40L与CRP一样有可能作为炎症指标,预测冠心病的发生及其预后。
目的
探讨血清OX40配体蛋白(OX40L)的水平与冠状动脉病变程度之间的相关性。
方法
选取行选择性冠状动脉造影术检查者共458例作为研究对象,根据冠状动脉造影结果冠状动脉病变的支数,分为1支病变组、2支病变组、3支病变组和冠脉造影正常的对照组(0支病变组),冠状动脉病变程度由冠状动脉病变支数和Gensini积分表示,采用ELISA法测定患者的血清OX40L的浓度,对OX40L水平与冠脉病变程度进行单因素和多因素分析。
结果
(1)冠状动脉病变各组OX40L的水平显著高于正常对照组(P<0.05或P<0.01),血清OX40L的水平与冠状动脉病变程度和Gensini积分呈正相关(P<0.01)。
(2)多元逐步回归分析结果显示在校正了年龄、高血压和高密度脂蛋白胆固醇(HDL-C)后,血清OX40L的水平与冠状动脉病变总积分(r=4.763,P
冠心病(CHD)患者的血浆OX40L的水平与冠状动脉病变程度密切相关,可能对冠心病有一定的预测和诊断意义。
目的
研究中国重庆地区汉族人群OX40配体蛋白基因(TNFSF4)的单核苷酸多态性在人群中的分布情况,并探讨该多态性与冠心病之间的关系。
方法
对453例患者进行选择性冠状动脉造影术,根据冠状动脉造影结果和临床资料分为二组:冠心病组262例和正常对照组191例,提取各组患者的全血基因组DNA,采用Taqman实时荧光定量PCR法检测TNFSF4 rs3850641(A/G)的单核苷酸多态性。
结果
(1)重庆地区汉族人群中存在TNFSF4 rs3850641(A/G)的单核苷酸多态性,在冠心病组中基因型分布为:AA型70.5%,AG型22.0%、GG型7.5%;在对照组基因型分布为:AA型69.8%,AG型20.8%、GG型9.4%。
(2)冠心病组和对照组之间的基因型频率及等位基因频率均无显著性差异(P>0.05)。
结论
OX40L基因rs3850641(A/G)的单核苷酸多态性与中国重庆地区汉族人群冠心病发病可能无关。
Objective
To study the Clinical significance of the level of serum OX40 ligand (OX40L) and C reactive protein(CRP) in patients with coronary atherosclerotic heart disease(CHD).
Methods
The level of OX40L was measured by enzyme-linked immunosorbent assay(ELISA) in patients with stable angina pectoris(SAP), acute coronary syndromes(ACS),and normal control group. The level of CRP was measured by immunoturbidimetric method,and the severity of coronary artery lesion was evaluated according to coronary angiography.
Results
(1)The levels of OX40L and CRP were significantly higher in acute coronary syndromes(ACS) group than those in stable angina pectoris(SAP) and control groups(P<0.01).
(2)The level of OX40L was positively associated with the level of CRP(r=0.780, P﹤0.01).
Conclusions
The levels of OX40 ligand and CRP are related with the CHD’severity.Serum OX40L and CRP were sensitive markers of inflammation and were helpful for assessment of the severity of disease and outcome prediction in patients with CHD.
Objective
To investigate the correlation of serum OX40 ligand level and severity of coronary lesion in coronary atherosclerotic heart disease (CHD) patients.
Methods
458 patients underwent coronary angiography were divided into 4 groups according to the number of affected blood vessels: normal control group, single vessel damage group, double and three vessels damage groups. The severity of coronary lesion was claasified by the number of affected blood vessels and the CHD Gensini cumulative index. Their level of OX40L was measured by enzyme-linked immunosorbent assay(ELISA).Both univariate and multivariate analyses were employed.
Results
(1)The OX40L serum levels of the CHD patients were significantly higher than those in control group (P<0.01～0.05). Univariate analysis revealed the number of affected vessels was increased along with the increasing OX40L serum level. OX40L levels were positively correlated with the severity of coronary lesion and the Gensini index (P<0.01).
(2) After adjusting co-variations such as ages,hypertension and plasma HDL-C levels,OX40L levels still showed correlation with the Gensini index significantly (r=4.763,P<0.01).
Conclusions
The level of OX40L had association with the severity of angiographic coronary artery disease,which may contribute to the early diagnosis of CHD and the disease prognostic indication.
Objective
To investigate the genotype distribution of TNFSF4 gene polymorphism and to study the relationship between TNFSF4 rs3850641(A/G) gene polymorphisms and coronary heart disease(CHD).
Methods
453 subjects were include:262 coronary heart disease patients(CHD group),191 normal subjects(normal control group).All subjects were confirmed by coronary angiography,and were genotyped for the gene polymorphism of TNFSF4 rs3850641(A/G) by TaqMan real-time PCR.
Results
(1)Han nationality population in Chongqing had TNFSF4 rs3850641(A/G) gene polymorphisms,the frequencies of AA,AG and GG genotypes were 70.5% ,22.0% and 7.5% in CHD group while ones were 69.8%, 20.8% and 9.4% in controls.
(2)There were no significant differences in frequencies of genotype and allele between controls and CHD cases(P>0.05).
Conclusions
TNFSF4 rs3850641(A/G) gene polymorphisms should be independent of occurrence of CHD in Han nationality population in Chongqing.
引文
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