外湿对造模脾虚小鼠过氧化与抗氧化影响的实验研究
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摘要
本实验对劳倦过度加饮食失节法研制的脾虚小鼠模型、模拟潮湿环境因素研制的外湿小鼠模型及脾虚兼外湿小鼠模型在机体过氧化与抗氧化方面进行了比较研究。旨在探讨单纯环境因素——外湿对正常小鼠和脾虚造模小鼠生物膜结构和功能改变的规律及其机制，从分子生物学角度揭示湿邪作用机体产生损伤的机理，同时探讨脾虚生湿与外湿的关系。
实验结果表明：三组模型小鼠均出现了体重的下降，丙二醛(malondialdehyde，MDA)和共轭双烯(conjugated dienes，CD)指标的升高，过氧化氢酶(Catalase，Cat)、超氧化物歧化酶(superoxid dismutase，SOD)和谷胱甘肽过氧化物酶(Glutathione peroxide，GSH-Px)指标的下降，说明各组模型小鼠均出现了脂质过氧化损伤和抗氧化酶活性的下降。三组模型小鼠MDA、CD升高程度及Cat、SOD、GSH-Px降低程度均以脾虚兼外湿组(D组)最为明显，说明脂质过氧化损伤程度和抗氧化酶活性下降程度以D组为最，表明外因即外湿与内因即脾虚协同作用，导致机体脂质过氧化损伤和抗氧化酶活性下降是机体发病的关键。
This experiment is a comparative study on the mice with spleen deficiency modeled through over-strain plus irregular diet, with exopathic dampness, with spleen deficiency plus exopathic dampness on bodies' change of peroxidation-antioxidation. The experiment aims to approach the laws and mechanisms of the change of the structure and function of the biomembrane in normal mice modeled
through single circumstances factor-exopathic dampness and spleen
deficiency, reveal that dampness damage organism after it invaded and probe into the relationship between-water retention due to spleen deficiency and exopathic dampness.
The results show that modeled mice in the three groups of all have the decrease of body weight , the increase of the contents of malondialdehyde (MDA) and conjugated dienes (CD), the decrease of the activity of catalase (Cat), superoxide dismutase (SOD) and glutathione peroxidase (GSHHPx). In these modeled mice in three groups, the group of spleen deficiency plus exopathic dampness (group D) is the greatest in the increasing degree of the contents of MDA and CD and the decreasing degree of the activity of Cat, SOD and GSH-Px, explaining the group D is the greatest in the impairing degree of lipid peroxidation and the decreasing degree of antioxidant enzyme, which indicates the impairing of lipid peroxidation and the decrease of the activity of the antioxidant enzyme by the co-ordinate act of exopathic factor (exopathic dampness) and endopathic factor (spleen deficiency )is the key to the occurrence of disease.
实验结果表明：三组模型小鼠均出现了体重的下降，丙二醛(malondialdehyde，MDA)和共轭双烯(conjugated dienes，CD)指标的升高，过氧化氢酶(Catalase，Cat)、超氧化物歧化酶(superoxid dismutase，SOD)和谷胱甘肽过氧化物酶(Glutathione peroxide，GSH-Px)指标的下降，说明各组模型小鼠均出现了脂质过氧化损伤和抗氧化酶活性的下降。三组模型小鼠MDA、CD升高程度及Cat、SOD、GSH-Px降低程度均以脾虚兼外湿组(D组)最为明显，说明脂质过氧化损伤程度和抗氧化酶活性下降程度以D组为最，表明外因即外湿与内因即脾虚协同作用，导致机体脂质过氧化损伤和抗氧化酶活性下降是机体发病的关键。
This experiment is a comparative study on the mice with spleen deficiency modeled through over-strain plus irregular diet, with exopathic dampness, with spleen deficiency plus exopathic dampness on bodies' change of peroxidation-antioxidation. The experiment aims to approach the laws and mechanisms of the change of the structure and function of the biomembrane in normal mice modeled
through single circumstances factor-exopathic dampness and spleen
deficiency, reveal that dampness damage organism after it invaded and probe into the relationship between-water retention due to spleen deficiency and exopathic dampness.
The results show that modeled mice in the three groups of all have the decrease of body weight , the increase of the contents of malondialdehyde (MDA) and conjugated dienes (CD), the decrease of the activity of catalase (Cat), superoxide dismutase (SOD) and glutathione peroxidase (GSHHPx). In these modeled mice in three groups, the group of spleen deficiency plus exopathic dampness (group D) is the greatest in the increasing degree of the contents of MDA and CD and the decreasing degree of the activity of Cat, SOD and GSH-Px, explaining the group D is the greatest in the impairing degree of lipid peroxidation and the decreasing degree of antioxidant enzyme, which indicates the impairing of lipid peroxidation and the decrease of the activity of the antioxidant enzyme by the co-ordinate act of exopathic factor (exopathic dampness) and endopathic factor (spleen deficiency )is the key to the occurrence of disease.
引文
[1]Ohkawa H, et al. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;9(?)-3(?).
[2]Pryor WA and Castle L. In:Methods in enzymology. Packer L ed. Vol105 New York:Academic press 1984;293.
[3]Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389.
[4]Makarevich OP and Golikool PP. Superoxide dismutase activity in patients during acute periods of various diseases. Lab Delo 1983;6:24.
[5]Austin L, et al. Determination of glutathione reductase and glutathione peroxidase.Anal Biochem 1988; 174:575.
[6]沈自尹．中医虚证辨证参考标准．中西医结合杂志，1986：(10)：598．
[7]张蕴琨，等．力竭性游泳对小鼠脑、肝、肌组织自由基代谢和血清CK、LDH活性的影响．中国运动医学杂志，1995；14(2)：69．
[8]陈孝银，等．湿阻证大鼠胃肠功能的改变及健脾祛湿方药的影响．四川中医，1999；17(1)：4．
[9]王昕，等．伤湿所致大白鼠脾阳虚证动物模型及其机理研究．辽宁中医杂志，1995；22(4)：187．
[10]严灿，等．湿阻证患者免疫调节功能的临床观察及其机理探讨．中国中医基础医学杂志，1998；4(6)：38．
[11]吴丽丽，等．自由基损伤与湿邪致病机理的探讨．江西中医学院学报，2001；13(1)：20．
[12]张六通，等．外湿大鼠关节、肺、大小肠和肝病理学研究．中国中医基础医学杂志，1996；2(3)：35．
[2]Pryor WA and Castle L. In:Methods in enzymology. Packer L ed. Vol105 New York:Academic press 1984;293.
[3]Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389.
[4]Makarevich OP and Golikool PP. Superoxide dismutase activity in patients during acute periods of various diseases. Lab Delo 1983;6:24.
[5]Austin L, et al. Determination of glutathione reductase and glutathione peroxidase.Anal Biochem 1988; 174:575.
[6]沈自尹．中医虚证辨证参考标准．中西医结合杂志，1986：(10)：598．
[7]张蕴琨，等．力竭性游泳对小鼠脑、肝、肌组织自由基代谢和血清CK、LDH活性的影响．中国运动医学杂志，1995；14(2)：69．
[8]陈孝银，等．湿阻证大鼠胃肠功能的改变及健脾祛湿方药的影响．四川中医，1999；17(1)：4．
[9]王昕，等．伤湿所致大白鼠脾阳虚证动物模型及其机理研究．辽宁中医杂志，1995；22(4)：187．
[10]严灿，等．湿阻证患者免疫调节功能的临床观察及其机理探讨．中国中医基础医学杂志，1998；4(6)：38．
[11]吴丽丽，等．自由基损伤与湿邪致病机理的探讨．江西中医学院学报，2001；13(1)：20．
[12]张六通，等．外湿大鼠关节、肺、大小肠和肝病理学研究．中国中医基础医学杂志，1996；2(3)：35．