锦灯笼的活性成分及质量评价研究
|
摘要
锦灯笼为茄科酸浆属植物Physalis alkekengi L.var.franchetii(Mast.)Makino,具有清热解毒,利咽,化痰,利尿等功效,在我国许多地区都有分布,是常用的清热解毒药,在临床上有很好的疗效。但锦灯笼的质量标准目前尚不完善。
本文在综述了酸浆属植物化学成分和锦灯笼药理活性研究的基础上,运用现代色谱分离技术对锦灯笼的化学成分进行了系统的分离,并对其中的酸浆苦味素类成分进行了体外抗肿瘤活性和构效关系的研究。在此基础上,采用HPLC法同时测定了锦灯笼中2种酸浆苦味素类主要成分的含量;同时研究了酸浆苦味素D单体在锦灯笼药材中的稳定性。
1锦灯笼化学成分研究
利用多种色谱分离技术,从锦灯笼宿萼的乙醇提取物中共分离得到37个化合物,经理化和光谱数据鉴定出其中31个化合物。其中酸浆苦味素类9个,黄酮类10个,酚类5个,苯丙素类3个,其他类型的化合物4个。在已鉴定的31个化合物中,有1个新化合物,即5α-羟基-25,27-二氢-4,7-二去氢-7-脱氧新酸浆苦味素A(5α-hydroxy-25,27-dihydro-4,7-didehydro-7-deoxyneophysalin A),11个为酸浆属内首次分离得到,1个为种内首次分得。
对从锦灯笼中分离得到的酸浆苦味素类化合物进行了体外抗肿瘤活性研究,结果显示:酸浆苦味素类化合物对人肝癌细胞系Bel 7402具有一定的生长抑制作用。其中,化合物PA-9对人肝癌细胞系Bel 7402的抑制率最高,化合物PA-12最低。但是化合物PA-9对人肝正常细胞系LO-2的细胞毒性也较大。
利用MTT法,通过用人前列腺癌细胞LNCAP,PC-3进行测试,发现从锦灯笼中分离得到的1个新化合物在不同浓度下都具有细胞生长抑制作用,IC_(50)值分别为:5.266,0.7285μg ml~(-1)。
2锦灯笼的质量评价研究
建立了HPLC-DAD法同时测定锦灯笼中2种酸浆苦味素类成分含量的方法,并且测定了20批锦灯笼药材、4批苦蘵、1批天泡果,以及锦灯笼药材不同部位、苦蘵药材不同部位、天泡果药材不同部位和锦灯笼鲜果及干果中2种酸浆苦味素的含量。实验结果表明:锦灯笼药材中酸浆苦味素D是主要的酸浆苦味素类成分,20批锦灯笼药材中酸浆苦味素D的平均含量为2.025mg/g(即0.202%),该成分可认定是锦灯笼的特征性成分,因此建议药典中应该相应的以这种成分为指标进行质量控制,是比较合理的;苦藏中酸浆苦味素A为主要成分,天泡果中未检出这2种成分。锦灯笼不同部位,宿萼中这2种成分的含量最高;整株植物中,酸浆苦味素D的含量最高,进一步说明锦灯笼中酸浆苦味素D为主要的酸浆苦味素类成分。苦蘵药材中仅检出酸浆苦味素A,且果实和宿萼中的含量最高;天泡果药材中2种酸浆苦味素均未被检出。锦灯笼鲜果中酸浆苦味素D的含量略高于干果。
3锦灯笼化学成分的稳定性研究
采用化学动力学方法研究了温度、pH值和光照等因素对酸浆苦味素D的化学稳定性的影响。实验结果表明,酸浆苦味素D在酸性、中性、弱碱性条件中均较为稳定,而随着pH值的增大,t_(1/2)骤然减小,酸浆苦味素D的降解速度加快,即其在强碱性条件下很不稳定。在加热试验中,根据阿累尼乌斯经验公式计算得到酸浆苦味素D的活化能＞100 kJ·mol~(-1),说明酸浆苦味素D在12小时内几乎不受到温度的影响。酸浆苦味素D在自然光中的半衰期要小于在紫外光照射下,说明酸浆苦味素D在自然光照射下的降解速度更快,且不适宜长时间光照。因此,建议酸浆苦味素D对照品应保存在避光的环境下,对照品溶液应现配现用。
同时对锦灯笼药材中酸浆苦味素D的稳定性进行了研究。实验结果表明,锦灯笼药材30%甲醇提取液中的酸浆苦味素D在酸性、中性、弱碱性溶液中均较为稳定,而在pH＞12.0的溶液中,则完全被破坏,溶液颜色由棕黄色迅速变为亮黄色。95℃以下的加热温度对酸浆药材中的酸浆苦味素D几乎无影响,降解反应活化能Ea为6722.2kJ·mol~(-1),说明降解反应很慢,在此条件下锦灯笼药材中的酸浆苦味素D很稳定。自然光及紫外光对锦灯笼药材中的酸浆苦味素D的稳定性无影响。
Jindenglong,Calyx seu fructus physalis,a commonly used Chinese herb,is the dried calyxes of Physalis alkekengi L.var.franchetii(Mast.) Makino(Family Solanaceae).Its action is considered as clearing away heat and toxic material, relieving sore throat,dissipating phlegm,diureses and so on.It is distributed in most areas of China and is used widely as a treatment for different illnesses.
Based on the review on the chemical constituents and pharmacological research of Physalis,the chemical constituents of Physalis alkekengi var.francheti and their antitumor activities were researched.On the basis of systematic research on its chemical constituents,the thesis focused on the quality-related topics of Jindenglong, such as(1)quantitative determination of 2 major physalins simultaneously in Jindenglong using HPLC method,(2) the stability study on physalin D.The results were summarized as below.
1 Chemical Constituents of Physalis alkekengi var.francheti
By means of many different chromatographic methods such as silica gel, sephadex LH-20 and ODS column chromatography,37 compounds have been separated from the calyxes of Physalis alkekengi var.francheti and 31 of them were identified by chemical and spectral methods,including 9 physalins,10 flavonoids,5 phenols,3 coumarins and 4 other miscellaneous compounds.Among them,there is one new compound identified as 5α-hydroxy-25,27-dihydro-4,7-didehydro-7-deoxyneophysalin A.11 compounds were isolated from the Physaliphorous for the first time,and 1 compound was found from this plant for the first time.
The antitumor activities of the new neophysalin and eight known physalins isolated from the calyxes of Physalis alkekengi var.francheti were tested.It was found that all physalins inhibited cell growth against Bel 7402 cell,in which compound PA-9 was the most potent and PA-11 was the most inpotent.However, compound PA-9 can also inhibite the normal human liver cells LO-2 seriously.
The cytotoxic activities of the new compound were evaluated according to MTT assay against the PC-3(human prostate cancer) and LNCaP(human prostate cancer) cell lines.The cytotoxicity data(IC_(50) values) are 5.266 and 0.7285μg ml~(-1), respectively.
2 Quality assessments on Physalis alkekengi var.francheti
A novel method has been developed for quantitative determination of 2 major physalins simultaneously by HPLC method in 20 samples of P.alkekengi var. francheti as well as 4 samples of P.angulata,1 sample of P.minima,different parts of P.alkekengi var.francheti、P.angulata and P.minima.
3 Stability and kinetics study of physalin D
The chemical dynamics of physalin D was investigated by using HPLC with DAD detection.The influence factors of pH value,temperature and light on the stability of physalin D in solution were carried out respectively.The results indicated that physalin D was degraded when pH value exceeded 10.The optimal stability condition was at normal temperature,pH＜10 and away from light.
本文在综述了酸浆属植物化学成分和锦灯笼药理活性研究的基础上,运用现代色谱分离技术对锦灯笼的化学成分进行了系统的分离,并对其中的酸浆苦味素类成分进行了体外抗肿瘤活性和构效关系的研究。在此基础上,采用HPLC法同时测定了锦灯笼中2种酸浆苦味素类主要成分的含量;同时研究了酸浆苦味素D单体在锦灯笼药材中的稳定性。
1锦灯笼化学成分研究
利用多种色谱分离技术,从锦灯笼宿萼的乙醇提取物中共分离得到37个化合物,经理化和光谱数据鉴定出其中31个化合物。其中酸浆苦味素类9个,黄酮类10个,酚类5个,苯丙素类3个,其他类型的化合物4个。在已鉴定的31个化合物中,有1个新化合物,即5α-羟基-25,27-二氢-4,7-二去氢-7-脱氧新酸浆苦味素A(5α-hydroxy-25,27-dihydro-4,7-didehydro-7-deoxyneophysalin A),11个为酸浆属内首次分离得到,1个为种内首次分得。
对从锦灯笼中分离得到的酸浆苦味素类化合物进行了体外抗肿瘤活性研究,结果显示:酸浆苦味素类化合物对人肝癌细胞系Bel 7402具有一定的生长抑制作用。其中,化合物PA-9对人肝癌细胞系Bel 7402的抑制率最高,化合物PA-12最低。但是化合物PA-9对人肝正常细胞系LO-2的细胞毒性也较大。
利用MTT法,通过用人前列腺癌细胞LNCAP,PC-3进行测试,发现从锦灯笼中分离得到的1个新化合物在不同浓度下都具有细胞生长抑制作用,IC_(50)值分别为:5.266,0.7285μg ml~(-1)。
2锦灯笼的质量评价研究
建立了HPLC-DAD法同时测定锦灯笼中2种酸浆苦味素类成分含量的方法,并且测定了20批锦灯笼药材、4批苦蘵、1批天泡果,以及锦灯笼药材不同部位、苦蘵药材不同部位、天泡果药材不同部位和锦灯笼鲜果及干果中2种酸浆苦味素的含量。实验结果表明:锦灯笼药材中酸浆苦味素D是主要的酸浆苦味素类成分,20批锦灯笼药材中酸浆苦味素D的平均含量为2.025mg/g(即0.202%),该成分可认定是锦灯笼的特征性成分,因此建议药典中应该相应的以这种成分为指标进行质量控制,是比较合理的;苦藏中酸浆苦味素A为主要成分,天泡果中未检出这2种成分。锦灯笼不同部位,宿萼中这2种成分的含量最高;整株植物中,酸浆苦味素D的含量最高,进一步说明锦灯笼中酸浆苦味素D为主要的酸浆苦味素类成分。苦蘵药材中仅检出酸浆苦味素A,且果实和宿萼中的含量最高;天泡果药材中2种酸浆苦味素均未被检出。锦灯笼鲜果中酸浆苦味素D的含量略高于干果。
3锦灯笼化学成分的稳定性研究
采用化学动力学方法研究了温度、pH值和光照等因素对酸浆苦味素D的化学稳定性的影响。实验结果表明,酸浆苦味素D在酸性、中性、弱碱性条件中均较为稳定,而随着pH值的增大,t_(1/2)骤然减小,酸浆苦味素D的降解速度加快,即其在强碱性条件下很不稳定。在加热试验中,根据阿累尼乌斯经验公式计算得到酸浆苦味素D的活化能＞100 kJ·mol~(-1),说明酸浆苦味素D在12小时内几乎不受到温度的影响。酸浆苦味素D在自然光中的半衰期要小于在紫外光照射下,说明酸浆苦味素D在自然光照射下的降解速度更快,且不适宜长时间光照。因此,建议酸浆苦味素D对照品应保存在避光的环境下,对照品溶液应现配现用。
同时对锦灯笼药材中酸浆苦味素D的稳定性进行了研究。实验结果表明,锦灯笼药材30%甲醇提取液中的酸浆苦味素D在酸性、中性、弱碱性溶液中均较为稳定,而在pH＞12.0的溶液中,则完全被破坏,溶液颜色由棕黄色迅速变为亮黄色。95℃以下的加热温度对酸浆药材中的酸浆苦味素D几乎无影响,降解反应活化能Ea为6722.2kJ·mol~(-1),说明降解反应很慢,在此条件下锦灯笼药材中的酸浆苦味素D很稳定。自然光及紫外光对锦灯笼药材中的酸浆苦味素D的稳定性无影响。
Jindenglong,Calyx seu fructus physalis,a commonly used Chinese herb,is the dried calyxes of Physalis alkekengi L.var.franchetii(Mast.) Makino(Family Solanaceae).Its action is considered as clearing away heat and toxic material, relieving sore throat,dissipating phlegm,diureses and so on.It is distributed in most areas of China and is used widely as a treatment for different illnesses.
Based on the review on the chemical constituents and pharmacological research of Physalis,the chemical constituents of Physalis alkekengi var.francheti and their antitumor activities were researched.On the basis of systematic research on its chemical constituents,the thesis focused on the quality-related topics of Jindenglong, such as(1)quantitative determination of 2 major physalins simultaneously in Jindenglong using HPLC method,(2) the stability study on physalin D.The results were summarized as below.
1 Chemical Constituents of Physalis alkekengi var.francheti
By means of many different chromatographic methods such as silica gel, sephadex LH-20 and ODS column chromatography,37 compounds have been separated from the calyxes of Physalis alkekengi var.francheti and 31 of them were identified by chemical and spectral methods,including 9 physalins,10 flavonoids,5 phenols,3 coumarins and 4 other miscellaneous compounds.Among them,there is one new compound identified as 5α-hydroxy-25,27-dihydro-4,7-didehydro-7-deoxyneophysalin A.11 compounds were isolated from the Physaliphorous for the first time,and 1 compound was found from this plant for the first time.
The antitumor activities of the new neophysalin and eight known physalins isolated from the calyxes of Physalis alkekengi var.francheti were tested.It was found that all physalins inhibited cell growth against Bel 7402 cell,in which compound PA-9 was the most potent and PA-11 was the most inpotent.However, compound PA-9 can also inhibite the normal human liver cells LO-2 seriously.
The cytotoxic activities of the new compound were evaluated according to MTT assay against the PC-3(human prostate cancer) and LNCaP(human prostate cancer) cell lines.The cytotoxicity data(IC_(50) values) are 5.266 and 0.7285μg ml~(-1), respectively.
2 Quality assessments on Physalis alkekengi var.francheti
A novel method has been developed for quantitative determination of 2 major physalins simultaneously by HPLC method in 20 samples of P.alkekengi var. francheti as well as 4 samples of P.angulata,1 sample of P.minima,different parts of P.alkekengi var.francheti、P.angulata and P.minima.
3 Stability and kinetics study of physalin D
The chemical dynamics of physalin D was investigated by using HPLC with DAD detection.The influence factors of pH value,temperature and light on the stability of physalin D in solution were carried out respectively.The results indicated that physalin D was degraded when pH value exceeded 10.The optimal stability condition was at normal temperature,pH＜10 and away from light.
引文
[1]中华人民共和国卫生部药典委员会.中华人民共和国药典一部[M].北京,化学工业出版社,2005:250-251.
[2]周静,王莉,李艳.酸浆果实营养成分分析[J].营养学报,1997,19(2):244-245.
[3]许亮,孙鹏,王冰,杨燕云.酸浆的本草考证[J].中医药学刊,2005,23(5):908-909.
[4]江苏新医学院.中药大辞典(下)[M].上海,上海科学技术出版社,1986:2532-2535.
[5]中华本草编委会.中华本草[M].上海,上海科学技术出版社,第7册.1999:286-290
[6]中国科学院中国植物志编辑委员会.中国植物志[M].北京,科学出版社,第67卷(第1分册).1987:53-59.
[7]Mstsuura T.,Kawai M.,Nakashima R.,Bitter principles of Physalis alkekengi var.franchetii:Structure of physalin A[J].Tetrahedron Lett.,1969,(14):1083-1086.
[8]Mstsuura T.,Kawai M.,Physalis alkekengi var.franchetii:Structure of physalin B[J].Tetrahedron Lett.,1969,(22):1765-1766.
[9]Kawai M.,Mstsuura T.,The structure of physalin C:A bitter principle of Physalis alkekengi var.franchetii[J].Tetrahedron,1970,26(7):1743-1745.
[10]Row Ramachandra L.,Reddy Sambi K.,Sarma Subrahmanya N.,Matsuura T.,Nakashima R.,New physalins form Physalis angulata and Physalis lancifolia.Stucture and reaction of physalins D,I,G and K[J].Phytochemistry,1980,19:1175-1181.
[11]Row Ramachandra L.,Sarma Subrahmanya N.,Matsuura T.,Nakashima R.,Physalins E and H,new physalins form Physalis angulata and Physalis lancifolia[J].Phytochemistry,1978,17:1641-1645.
[12]Row Ramachandra L.,Sarma Subrahmanya N.,Reddy Sambi K.,Matsuura T.,Nakashima R.,The structure of physalins F and J form Physalis angulata and Physalis lancifolia[J].Phytochemistry,1978,17:1647-1650.
[13]Kawai M.,Mstsuura T.,Kyuno S.,Matsuki H.,Takenaka M.,Katsuoka T.,Butsugan Y.,Saito K.,A new physalin from Physalis alkekengi:structure of physalin L[J].Phytochemistry,1987,26(12):3313-3317.
[14]Kawai M.,Ogura T.,Nakanishi M.,Matsuura T.,Butsugan Y.,Mori Y.,Harada K.,Structure of physalin M isolated from Physalis alkekengi var.franchetii[J].Bull.Chem.Soc.Jpn.,1988,61:2696-2698.
[15]Kawai M.,Ogura T.,Makino B.,Matsumoto A.,Yamamura H.,Butsugan Y.,Hayashi M.,Physalin N and O from Physalis alkekengi[J].Phytochernistry,1992,31(12):4299-4302.
[16]Makino B.,Kawai M.,Yasushi I.,Yamamura H.,Butsugan Y.,Ogawa K.,Hayashi M.,Physalins possessing an endoperoxy structure from Physalis alkekengi var.franchetii.Structural revision of physalin K[J].Bull.Chem.Soc.Jpn.,1995,68:219-226.
[17]Makino B.,Kawai M.,Kito K.,Yamamura H.,Butsugan Y.,New physalins possessing an additional carbon-crabon bond from Physalis alkekengi var.franchetii[J].Tetrahedron,1995,51(46):12529-12538.
[18]Makino B.,Kawai M.,Ogura T.,Nakanishi M.,Yamamura H.,Butsugan Y.,Structural revision of physalin H isolated from Physalis angulata[J].Journal of Natural Products,1995,58(11):1668-1674.
[19]Kawai M.,Yamamoto T.,Makino B.,Yamamura H.,Araki S.,Butsuqan Y.,Saito K.,The structure of physalin T from Physalis alkekengi var.franchetii[J].J Asian Nat Prod Res,2001,3(3):199-205.
[20]Sunayama R.,Kuroyanagi M.,Umehara K.,Ueno A.,Physalin and neophysalins from Physalis alkekengi var.franchetii and their differentiation inducing activity[J].Phytochemistry,1993,34(2):529-533.
[21]陈荣,梁敬钰,杨洋,刘睿,酸浆化学成分及酸浆苦素G的结构订正[J].中国天然药物,2007,5(3):186-189.
[22]Ping-Chung Kuo,Tsung-Hsiao Kuo,Amooru G.Damu,Chung-Ren Su,E-Jian Lee,Tian-Shung Wu,Rexen Shu,ChouoMing Chen,Kenneth F.Bastow,Tzu-Hsuan Chen,Kuo-Hsiung Lee,Physanolide A,a novel skeleton steroids,and other cytotoxic principles from Physalis angulata[J].Organic Letters,2006,8(14):2953-2956.
[23]Amooru G.Damu,Ping-Chung Kuo,Chung-Ren Su,Tsung-Hsiao Kuo,Tzu-Hsuan Chen,Kenneth F.Bastow,Kuo-Hsiung Lee,Tian-Shung Wu,Isolation,structure,and structure-cytotoxic activity relationships of withanolides and physalins from Physalis angulata[J].J.Nat.Prod.,2007,(70):1146-1152.
[24]Kawai M.,Ogura T.,Butsugan Y.,Taga T.,Hayashi M.,Benzilic acid rearrangement-type reaction of physalins to neophysalins.Structural revision of one of the dehydration products of physalin A[J].Tetrahedron,1991,47(12/13):2103-2110.
[25]Kawai M.,Matsumoto A.,Makino B.,Mori H.,Ogura T.,Butsugan Y.,Ogawa K.,Hayashi M.,The structure of physalin P,a neophysalin from Physalis alkekengi[J].Bull.Chem.Soc.Jpn.,1993,66:1299-1300.
[26]Rong Chen,Jing-Yu Liang,Rui Liu,Two novel neophysalins from Physalis aIkekengi var.franchetii[J].Helvetica Chimica Acta,2007,90:963-966.[27]Shinya Nagafuji,Hikaru Okabe,Hiroshige Akahane et al.,Trypanocidal constituents in plant withanolides from the aerial parts of Physalis angulata[J].Bio.Pharm.Bull.,2004,27:193-197.
[28]O.E.Vasina,N.D.Abdullaev,N.K.Abubakirov,Withanolides of physalis Ⅸ physangulide-the first natural 22s-withasteroid[J].Chem.Nat.Compd.,1990,26:304-307.
[29]Saeed Ahmad,Abdul Malik,Nighat Afza et al.,A new withanolide glycoside from Physalis peruvina[J].J.Nat.Prod,1990,62:493-494.
[30]G.L.Silva,A.Pacciaroni,J.C.Oberti et al.,A pregnane structurally related to withanolides Physalis viscose[J].Phytochemistry,1993,34:871-873.
[31]Anothony C.Waiss Jr.,Carl A.Euiger,William F.Haddon et al.,Insect inhibitory steroidal saccharide esters from Physalisperuvina[J].J.Nat.Prod.,1993,56:1365-1372.
[32]Kazushi Shingu,Shaji Yahara,Toshihiro Nohara et al.,Three new withanolides,physalin A,B and D from Physalis angulata[J].Chem.Pharm.Bull.,1992,40(8):2088-2091.
[33]Chiu-Ming Chen,Zong-Tsi Chen,Chiu-Hsiang Hsieh et al.,Withangulatin A,a new withanolide from Physalis angulata[J].Heterocycles,1990,31(7):1371 - 1375.
[34]Kazushi Shingu,Shoji Yahara,Hikaru Okabe et al.,Three new withanolides physalins E,F and G from Physalis angulata L.[J].Chem.Pharm.Bull.,1992,40(9):2448-2451.
[35]Kazushi Shingu,Mariko Miyagawa,Shoji Yahara et al.,Physapruins A ans B,two new withanolides from Physalis pruinosa Bailey[J].Chem.Pharm.Bull.,1993,41(10):1873-1875.
[36]Saeed Ahmad,Abdul Malik,Rehana Yasmin,Withanolides from Physalis peruviana[J].Phytochemistry,1999,50:647-651.
[37]Laurence N.Dinah,Satyajit D.Sarker,Vladimir Sik,28-hydroxy withanolide E from Physalis peruviana[J].Phytochemistry,1997,44(3):509-512.
[38]Kazushi Shingu,Nobuhiro Marubayashi,Ikuhiko Veda et al.,Physapruins C,a new withanolide from Physalis angulata L.[J].Chem.Pharm.Bull.,1991,39(6):1591-1593.
[39]Saeed Ahmad,Pehana Yasmin,Abdul Malik,New withanolide glycosides from Physalis perubiana L.[J].Chem.Pharm.Bull.,1999,47(4):477-480.
[40]Emma Maldonado,Fernando R.Torres,Mahinda Matinez et al.,18-acetoxywithanolides from Physalis chenopodifolia[J].Planta Med.,2004,70:59-64.
[41]Perez-Castorena,A.-L.,Oropeza,R.F.,Vazquez,A.R.,Martinez,M.,Maldonado,E.Labdanes and withanolides from Physalis coztomatl[J].J.Nat.Prod.,2006,69(7):1029-1033.
[42]Itoh T.,Butsugan Y.,[J].Phytochemistry,1974,13(10):2239-2241.
[43]N.K.Sharma,D.K.Kulshreshtha,J.S.Yandon et al.,[J].Phytochemistry,1974,13(10):2239-2245.
[44]L.Ramachandta Row,K.Sambi Reddy,N.Subtahsiana Samna et al.,[J].Phytochemistry,1980,19(8):1175-1181.
[45]Itoh T.,Teruo M.,Ruka N.et al.,[J].Phytochemistry,1977,16(11):1723-1728.
[46]李静,李娟,李德坤,锦灯笼化学成分的研究Ⅰ[J].中草药,2002,33(8):692-693.
[47]王明东,杨松松,锦灯笼化学成分及药理作用综述[J].辽宁中医学院学报,2005,7(4):341-342.
[48]梁慧,才谦,锦灯笼果实化学成分的研究[J].中华中医药学刊,2007,25(8):1677-1679.
[49]赵倩,邱莉,卜光明,曲戈霞,邱峰,酸浆宿萼的化学成分[J].沈阳药科大学学报,2006,23(3):151-155.
[50]邱莉,姜志虎,刘红霞,陈丽霞,曲戈霞,邱峰,酸浆宿萼的黄酮苷类化学成分[J].沈阳药科大学学报,2007,24(12):727-730.
[51]马哲.锦灯笼宿萼化学成分的分离与鉴定.辽宁中医药大学硕士毕业论文.2007.5
[52]Ser N.A.,Flavonoids from Physalis minima[J].Phytochemistry,1988,27(11):3708-3709.
[53]N.Ismail,M.Alam,A novel cytotoxic flavonoid glycoside from Physalis angulata[J].Fitoterapia,2001,72:676-679.
[54]Naoki Asano,1β-amino-2α,3β,5β-trihydroxycycloheptane from Physalis alkekengi var.franchetii[J].Phytochemistry,1996,42(3):719-721.
[55]Hideo Y.,Kuniko N.,Studies on the alkaloids of the roots ofPhysalis alkekengi.Isolation of 3α-tigloyloxytropane[J].Chem.Pharm.Bull.,1965,13(2):217-220.
[56]Naoki A.,Atsushi K.,Kengo O.,Haruhisa K.,Katsuhiko M.,Calystrgins of Physalis alkekengi var.franchetii(Solanaceae) Structure determination and their glycosidase inhibitory activities[J].Eur J.Biochem.,1995,229:369-376.
[57]Keith Basey,Brian A.Mcgaw,Jack G.Woolley,Phygrine,an alkaloid from Physalis species[J].Phytochemistry,1992,31(12):4173-4176.
[58]周静,王莉,李艳,鲁建疆,酸浆果实营养成分分析[J].营养学报,1997,19(2):243-245.
[59]吕春平,李娟,吕冬梅,锦灯笼中无机元素含量测定[J].广东微量元素科学,2000,7(6):55-56.
[60]李泽鸿,郝殿明,刘树英,刘洪章,锦灯笼宿萼中营养元素的测定研究[J].世界元素医学,2007,14(4):50-52.
[61]杨晓虹,田丁丁,周小平,酸浆宿萼无机元素和氨基酸含量的测定[J].白求恩医科大学学报,2001,27(1):17-18.
[62]葛玉,段玉峰,刘俊花,肖红,黑龙江酸浆果实及宿萼的成分分析[J].营养学报,2006,28(6):529-532.
[63]蕙伯棣,王亚静,裴凌鹏,李京,张艳,酸浆浆果和宿萼中类胡萝卜素组成分析[J].中国药学杂志2006,41(8):583-585.
[64]韩阳花,酸浆有效成分的初步研究.新疆大学硕士学位论文,2005.
[65]赵倩,董艳丽,曲戈霞,邱峰,酸浆宿萼挥发性成分的研究[J].中药研究与信息,2005,7(4):183-184.
[66]韩阳花,高莉,刘丽艳,酸浆果实中多糖的提取及含量测定[J].食品科学,2006,27(2):154-157.
[67]刘鹏举,薛小刚,马镝,阚国仕,水溶性酸浆果多糖的提取及清除自由基作用的研究[J].食品科技,2007,(12):96-98.
[68]王萍,付勇,苗雨,毛酸浆果实多糖的提取[J].食品工业科技,2008,(2):204-206.
[69]葛玉,段玉峰,刘俊花,刘平,酸浆果多糖不同提取工艺研究[J].食品科学,2006,27(8):178-183.
[70]周静,李艳,酸浆果多糖的提取及含量测定[J].数理医药与杂志,2000,13(3):242-243.
[71]葛玉,段玉峰,刘俊花,张欣,酸浆果萼水溶性多糖提取工艺的比较[J].东北林业大学学报,2006,34(5):73-75.
[72]李娟,李静,锦灯笼化学成分的研究Ⅱ[J].中草药,2002,33(9):788-789.
[73]武海燕,索全伶,李俊,药用植物酸浆的研究进展[J].内蒙古石油化工,2007,(2):5-8.
[74]程瑛琨,李蕾,王丹梅,滕利荣,酸浆宿萼色素的提取工艺及稳定性的研究[J].食品工业科技,2006,27(12):58-62.
[75]甄清,李静,李勇,李文君,李娟,锦灯笼宿萼提物体外抗菌作用研究[J].天然产物研究与开发,2006,(18):273-274.
[76]袁昌衡,周启贵,杨飞中,80种中药水煎液对淋球菌的抑制试验[J].中国药学杂志,1997,17(11):508-509.
[77]宋立人,现代中药学大词典(下册).北京,人民卫生出版社,1999,2311-2314.
[78]李萍,盛巡,王晓中,张建华,鲍朝辉,何延良,丁瑞,酸浆苦素B对活化多形核中性粒细胞化学发光及H_2O_2产生的影响[J].中草药,1996,27(5):284-287.
[79]李萍,盛巡,王晓中,活化嗜中性粒细胞[Ca~(2+)]Ⅰ的变化及锦灯笼酸浆苦素B的影响[J].中国病理生理杂志,1996,12(6):660.
[80]王灿岭,翁何霞,酸浆水提取物的抗炎作用研究[J].中医研究,2007,20(1):17-18.
[81]吕春平,王宏芳,李静,李娟,锦灯笼抗炎作用实验研究[J].现代预防医学,2007,34(12):2213-2215.
[82]李德坤,锦灯笼化学成分的研究.吉林大学硕士学位论文,2001.
[83]吉林省中医中药研究所,长白山植物药志[M].吉林人民出版社1982:100-105.
[84]汪丽燕,木犀草素对冠脉血流动力学的实验研究[J].中国病理学通报,1992,8(5):388-390.
[85]王和平,徐美术,孙亮,宋旭波,锦灯笼降血糖作用的实验研究[J].中医药信息,2004,21(1):53-54.
[86]宋旭波,锦灯笼制剂及其生物活性研究.黑龙江中医药大学硕士学位论文,2003.
[87]李芳莲,范恩学,徐丹,邵静,中药挂金灯的降血脂作用实验研究[J].中国老年学杂志,2006,26(1):91-93.
[88]Antoun MD.,Abramson D.,Tyson RL.,Potential antitumor agents.ⅩⅦ.physalin B and 25,26-epidihydrophysalin C from Witheringia coccoloboides[J].J Nat.Prod.,1981,44:579-585.
[89]Han R.,Recent progress in the study of anti-cancer drugs originating from plants and traditional medicines in China[J].J Chin.Med.Sci.,1994,9:61-69.
[90]Dornberger K.,The potential antineoplastic acting constituents of Physalis alkekengi vat.frenchetii Mast.[J].Pharmazie,1986,41(4):265-268.
[91]Lei Ma,Mumtaz All,Mohammad Arfan,Li-Guang Lou,Li-Hong Hu,Withaphysanolide A,a novel C-27 norwithanolide skeleton,and other cytotoxic compounds from Physalis divericata[J].Tetrahedron Letters,2007,48:449-452.
[92]Lei Ma,Chuan-Ming Xie,Jia Li,Feng-Chang Lou,Li-Hong Hu,Daturametelins H,I,and J:three new withanolide glycosides from Datura metel L.[J].Chemistry & Biodiversity,2006,3:180-186.
[93]Muhammad Iqbal C.,Sammer Y.,Samreen,Syed Adnan Ali S.,Shakil A.,Atta-UR-Rahman,Biotransformation of physalin H and leishrnanicidal activity of its transformed products[J].Chem.Pharm.Bull.,2006,54(7):927-930.
[94]Muhammad lqbal C,,Sammer Y.,Samreen,Syed Adnan Ali S.,Shakil A.,Atta-UR-Rahman,Antileishmanial physalins from Physalis minima[J].Chemistry & Biodiversity,2005,2:1164-1173.
[95]A.H.Januario,E.Rodrigues,R.C.L.R.Pietro,S.Kashima,D.N.Sata,S.C.Franca,Antimycobacterial physalins from Physalis angulata L.(Solanaceae)[J].Phytotherapy Research,2002,16:445-448.
[96]Eloi S.Garcia,Daniele P.Castro,Ivone M.Ribeiro,Therezinha C.B Tomassini,Patricia Azambuja,Trypanosama rangeli:Effects of physalin B on the immune reactions of the infected larvae of Rhodnius prolixus[J].Experimental Parasitology,2006,112:37-43.
[97]Milena B.P.Soares,Moema C.Bellintani,Ivone M.Ribeiro,Therezinha C.B.Tomassini,Ricardo Ribeiro dos Santos,Inhibition of macrophage activation and lipophysaccaride-induced death by seco-steroids purified from Physalis angulata L.[J].European Journal of Pharmacology,2003,459:107-112.
[98]Lei Ma,Xian-Wen Gan,Qing-ping He,Hai-yun Bai,Mohammad Arfan,Feng-chang Lou,Li-hong Hu,Cytotoxic withaphysalins from Physalis minima[J].Helvetica Chimica Acta,2003,90:1406-1419.
[99]M.B.P.Soares,D.Brustolim,L.A.Santos,M.C.Bellintani,F.P.Paiva,Y.M.Ribeiro,T.C.B.Tomassini,R.Ribeiro dos Santo,Physalins B,F,and G,seco-steroids purified from Physalis angulata L.,inhibit lymphocyte function and allogeneic transplant rejection[J].International Immunopharmacology,2006,6:408-414.
[100]Nadia J.Jacobo-Herrera,Paul Bremner,Nieves Marquez,Mahabir P.Gupta,Simon Gibbons,Eduardo Munoz,Michael Heinrich,Physalins from Witheringia solanacea as mudulators of the NF-κB cascade[J].J.Nat.Prod.,2006,69:328-331.
[101]Liqiong Fang,Hee-Byung Chai,Juan J.Castillo,Djaja D.Soejarto,Norman R.Rarnsworth, Geoffrey A.Cordell,John M.Pezzuto,A.Douglas Kinggom,Cytotoxic counstituents of Brachistus stramoniifolius[J].Phytotherapy Research,2003,17:520-523.
[102]龚珊,单立冬,张玉英,郭试瑜,挂金灯镇痛作用的实验观察[J].苏州大学学报(医学版),2002,22(4):380-382.
[103]裴凌鹏,董福慧,孔焕宇,惠伯棣,酸浆提取物改善老龄大鼠抗氧化功能的研究[J].北京中医药大学学报,2007,30(10):688-690.
[104]杨春荣,单静颖,郑素芹,锦灯笼提取物对DPPH自由基清除作用的探讨[J].黑龙江医药科学,2008,31(3):42.
[105]刘寿山,中药文献研究摘要(1920-1961),1963:742.
[106]Vessal M.,Mehrani HA.,Omrani GH.,Effects of an aqueous extract of Physalis alkekengi fruit on estrus cycle,reproduction and uterine creation kinase BB-isozymein rats[J].J.Ethnopharmacol,1991,34(1):69-78.
[107]帕提古丽,马合木提,高莉史博,酸浆花萼色素的提取及理化性质研究[J].食品科学,2004,25(9):35.
[108]王玮,锦灯笼的营养保健功能及药用价值[J].中国食物与营养,2008,(3):54-56.
[109]曹妹芳,刘瑾,张剑华,急咽口服液质量标准研究[J].中成药,1999,21(1):46-47.
[110]杨建刚,清咽利喉方治疗急性咽炎40例[J].吉林中医药,2001,(4):33.
[111]周玲,酸浆果药用食疗方[J].饮食指南,2005,(4):71.
[112]廖木兰,灯笼草外治寻常疣[J].新中医,2004,36(8):74.
[113]郭文扬,酸浆的利用及栽种[J].特种经济动植物,2001,(1):30.
[114]武海燕,索全伶,李俊,药用植物酸浆的研究进展[J].内蒙古石油化工,2007,(2):5-8.
[115]王家东,王荣荣,酸浆开发利用研究的进展[J].农技服务,2007,24(11):109一112.
[116]谭雨清,孟晓敏,刘振扬,红姑娘(挂金灯)原汁饮料加工[J].食品科技,2003,(12):64-66.
[117]王立江,匡明,红姑娘果醋表面发酵法工艺的研究[J].江苏调味副食品,2006,(2):121-125.
[118]张丽,刘奉先,薄层扫描法测定锦灯笼中酸浆果素的含量[J].中医药信息,2000,(5):56.
[119]殷莉梅,韩静,潘岳峰,李智,单娜,锦灯笼含片娇味工艺的研究[J].中国中药杂志,2008,33(13):1635-1637.
[120]杨文菊,敬思群,杨洁,新疆酸浆宿萼总黄酮含量测定方法的研究[J].新疆大学学报(自 然科学版),2007,24(3):339-341.
[121]敬思群,郑力,苏俊,酸浆宿萼总黄酮和多糖的提取及含量测定[J].食品研发与开发,2008,29(6):82-84.
[122]敬思群,阿依努尔,艾合买提江,酸浆宿萼总黄酮提取工艺的研究[J].新疆大学学报,2008,25(1):88-91.
[123]Sen G.,Mulchandani N.B.,Patankar A.V.,Reversed-phase high performance liquid chromatographic separation and analysis of some physalins(13,14-seco- 16,24-cyclo-steroids)[J].Journal of Chromatography,1980,198(2):203-206.
[124]于婷,刘玉强,穆春旭,冯雪,赵红丹,林长旭,才谦,锦灯笼果实中4,7-二脱氢新酸浆苦素B的含量测定[J].辽宇中医杂志,2008,35(4):584-586.
[125]赵红丹,林长旭,尹程鑫,于婷,冯雪,才谦,锦灯笼宿萼醇提物中4,7-二脱氢新酸浆苦素B的含量测定[J].辽宁中医药大学学报,2008,10(4):129-131.
[126]许亮,徐秋阳,王冰,王荣祥,高效液相色谱法测定锦灯笼药材中木犀草素的含量研究[J].时珍国医国药,2006,17(4):575-576.
[127]徐保利,王冰,辽宁各地酸浆宿存萼中木犀草素含量测定[J].山西中医学院学报,2008,10(4):50-52.
[128]王和平,张晓燕,宋旭波,李艳凤,李秋红,HPLC法测定锦灯笼口服液中木犀草素的含量[J].黑龙江医药,2004,17(1):10-12.
[129]许亮,刘红,于荣军,杨燕云,王冰,康廷国,锦灯笼配方颗粒中木犀草素的HPLC 法含量测定[J].中华中医药学刊,2008,26(3):653-654.
[130]施蕊,贾凌云,孙启时,于丹丹,RP-HPLC测定酸浆药材中3种有效成分的含量[J].药物分析杂志,2008,28(2):260-263.
[131]施蕊,贾凌云,孙启时,酸浆药材及其提取物高效液相色谱特征[J].中国中药杂志,2007,32(20):2194-2196.
[132]许亮,王冰,贾天柱,锦灯笼与兔儿伞两种药材的挥发油成分研究[J].中成药,2007,29(12):1840-1843.
[1]Reiko Sunayama,Masanori Kuroyanagi,Kaoru Umehara,Akira Ueno.Physalin and neophysalins from Physalis alkekengi var.francheti and their differentiation inducing activity[J].Phytochemistry,1993,34(2):529-533.
[2]Masao Kawai,Akihide Matsumoto,Bunsho Makino,Hideyuki Mori,Toichi Ogura,Yasuo Butsugan,Kiyoshi Ogawa,Mitsuo Hayashi.The structure of physalin P,a neophysalin from Physalis alkekengi[J].The Chemical Society of Japan,1993,(66):1299-1300.
[3]Liqiong Fang,Hee Byung Chai,Juan J.Castillo,Djaja D.Soejarto,Norman R.Farnsworth,Geoffrey A.Cordell,Johm M.Pezzuto,A.Douglas Kinghorn.Cytotoxic constituents of Brachistus stramoniifolius[J].Phytotherapy Research,2003,(12):520-523.
[4]Masao Kawai,Toichi Ogura,Bunsho Makino,Akihide Matsumoto,Hatsuo Yamamura,Yasuo Butsugan,Mrrsuo Hayashi.Physalins N and O from Physalis alkekengi[J].Phytochemistry,1992,31(12):4299-4320.
[5]Bunsho Makino,Masao Kawai,Toichi Ogura,Masaki Nakanishi,Hatsuo Yamamura,Yasuo Butsugan.Structural revision of physalin H isolated from Physalis angulata[J].Journal of NaturalProducts,1995,58(11):1668-1674.
[6]Masao Kawai,Toichi Ogura,Yasuo Butsugan,Tooru Taga,Mitsuo Hayashi.Benzilic acid rearrangement-type reaction of physlins to neophysalins.Structure revision of one of the dehydroation products of physalin A[J].Tetrahedron,1991,47(12):2103-2110.
[7]L.Ramachandra Row,N.Subrahmanya Sarrna,Teruo Matsuura,Ruka Nakashima.Physalins E and H,new physalins from Physalis angulata and P.lancifolia[J].Phytochemistry,1978,(17):1641-1645.
[8]贾凌云,孙启时,黄顺旺.滁菊花中黄酮类化学成分的分离与鉴定[J].中国药物化学杂志2003,13(3):159-161.
[9]王宇杰,孙启时.金钱草的化学成分研究[J].中国药物化学杂志,2005,15(6):357-359.
[10]周凌云,张祥华,陈昌祥.丽江产红景天的化学成分研究[J].天然产物研院与开发,2004,16(5):410-414.
[11]何桂霞,裴刚,周天达,周雪仙.瑶族藤茶中杨梅素的分离与结构鉴定[J].中国民族医药杂志,2000,6(1):40-42.
[12]Jorge M.David,Frederico G.Cruz,Maria Lenise S.Guedes,Juceni P.Chavez.Flavonol glycosides from Davilla flexuosa[J].J.Braz.Chem.Soc.,1996,7(2):115-118.
[13]梁侨丽.扁豆花化学成分研究[J].中国药科大学学报,1996,27(4):206-207.
[14]邱鹰昆,窦德强,裴玉萍,吉川雅之,松田久司,陈英杰.仙人掌的化学成分研究[J].中国药科大学学报,2005,36(3):213-215.
[15]张思巨,王怡薇,刘丽,于江泳,扈继萍.锁阳化学成分研究Ⅱ[J].中国药学杂志,2007,42(13):974-976.
[16]杨维力,田军,丁立生.白苞裸蒴的化学成分[J].中国中药杂志,2001,26(1):43-45.
[17]赵东保,张卫,李明静,刘绣华.项羽菊化学成分研究[J].中国中药杂志,2006,31(22):1869-1872
[18]Christina Vafiadi,Evangelos Topakas,Ken K.Y.Wong,Ian D.Suckling,Paul Christakopoulos.Mapping the hydrolytic and synthetic selectivity of a type C feruloyl esterase (StFaeC) from Sporotrichum thermophile using alkyl ferulates[J].Tetrahedron:Asymmetry,2005,(16):373-379.
[19]董礼,李磊,廖志华,陈敏,孙敏.柴胡红景天化学成分的研究[J].西北植物学报,2007,27(12):2564-2567.
[20]肖永庆,李丽,游小琳,谷口雅颜.川芎化学成分研究[J].中国中药杂志,2007,27(7):519-521.
[21]章为,徐康平,周应军,谭桂山.两色紫金牛化学成分研究[J].中南药学,2007,5(5):444-448.
[22]姚巍,林文艳,周长新,赵昱.蒙古蒲公英化学成分研究[J].中国中药杂志2007,32(10):926-928.
[23]Kadali S.Sagar,Chia-Chuan Chang,Wei-Kung Wang,Jung-Yaw Lin and Shoei-Sheng Lee,Preparation and anti-HIV activities of retrojusticidin B analogs and azalignans[J].Bioorganic and Medical Chemistry,2004,(12):4045-4054.
[24]古海峰,陈若芸,孙玉华,邢建国.香青兰化学成分研究Ⅱ[J].中国中药杂志,2005,30(9):677-679.
[25]Jesper Jarsen,Dan Staerk,Claus Comett,Steen H.Hansen,Jerzy W.Jaroszewski.Identification of reaction products between drag substances and excipients by HPLC-SPE-NMR:Ester and amide formation between citric acid and 5-aminosalicylic acid[J].Journal of Pharmaceutical and Biomedical Analysis,2009,(49):839-842.
[1]Sen G.,Mulchandani N.B.,Patankar A.V.,Reversed-phase high performance liquid chromatographic separation and analysis of some physalins(13,14-seco-16,24-cyclo-steroids)[J].Journal of Chromatography,1980,198(2):203-206.
[2]于婷,刘玉强,穆春旭,冯雪,赵红丹,林长旭,才谦,锦灯笼果实中4,7-二脱氢新酸浆苦素B的含量测定[J].辽宁中医杂志,2008,35(4):584-586.
[3]赵红丹,林长旭,尹程鑫,于婷,冯雪,才谦,锦灯笼宿萼醇提物中4,7-二脱氢新酸浆苦素B的含量测定[J].辽宇中医药大学学报,2008,10(4):129-131.
[4]许亮,徐秋阳,王冰,王荣祥,高效液相色谱法测定锦灯笼药材中木犀草素的含量研究[J].时珍国医国药.2006,17(4):575-576.
[5]徐保利,王冰,辽宁各地酸浆宿存萼中木犀草素含量测定[J].山西中医学院学报,2008,10(4):50-52.
[6]王和平,张晓燕,宋旭波,李艳凤,李秋红,HPLC法测定锦灯笼口服液中木犀草素的含量[J].黑龙江医药,2004,17(1):10-12.
[7]许亮,刘红,于荣军,杨燕云,王冰,康廷国,锦灯笼配方颗粒中木犀草素的HPLC法含量测定[J].中华中医药学刊,2008,26(3):653-654.
[8]施蕊,贾凌云,孙启时,于丹丹,RP-HPLC测定酸浆药材中3种有效成分的含量[J].药物分析杂志,2008,28(2):260-263.
[9]施蕊,贾凌云,孙启时,酸浆药材及其提取物高效液相色谱特征[J].中国中药杂志,2007,32(20):2194-2196.
[1]Yue X D,Qu G W,Li G S,et al.Stability of salvianolic acid B in water solution[J].Chinese Traditional and Herbal Drugs,2005,36(2):49-51.
[2]Ni L J,Wu K F,Zhang L G.Influence of Heating Method on the Quality of Extracts from Radix Salviae Miltiorrhizae[J].Traditional Chinese Drug Research & Clinical Pharmacology,2006,17(1):56-58.
[3]Lin H B,Lin H K.Study on the stability of Tanshinone ⅡA[J].Traditional Chinese Medicinal Research,2005,18(8):19.
[4]Lu W B,Zeng Y E,Zhong J J,et al.Research on the Stability Factors of Tanshinone Ⅱ_A in Preparation of Salvia miltiorrhiza Bge[J],Lishizhen Medicine and Medica Research,2002,11(1):10-12.
[5]胡春霞,佟凤琴,酸浆果皮色素的提取及稳定性研究[J].辽宁师专学报,2001,3(2):91-93.
[6]李萍,盛巡,王晓中,张建华,鲍朝辉,何延良,丁瑞,酸浆苦素B对活化多形核中性粒细胞化学发光及H_2O_2产生的影响[J].中草药,1996,27(5):284-287.
[7]李萍,盛巡,王晓中,活化嗜中性粒细胞[Ca~(2-)]I的变化及锦灯笼酸浆苦素B的影响[J].中国病理生理杂志,1996,2(6):660.
[8]王灿岭,翁何霞,酸浆水提取物的抗炎作用研究[J].中医研究,2007,20(1):17-18.
[9]吕春平,王宏芳,李静,李娟,锦灯笼抗炎作用实验研究[J].现代预_防医学,2007,34(12):2213-2215.
[10]Fu X C,Shen W X,Yao T Y.Physical chemistry(物理化学),4~(th) Ed.,Beijing,Higher Education Press,1990.
[11]Li X F,Fu J,Jin M Z.Study on degradation dynamics of baicalin in the extraction of Radix Sculellariae by water.Jourmal of Modern Food and Pharmaceticals(现代食品与药品杂志),2006,16(4):32-34.
[12]Xu Z S,Cai B C,Zhang X.Research of the stability of ferulic acid in Rhizoma ChuanXiong.Research and Practice of Chinese Medicines(现代中药研究与实践),2004,18(2):24-26.
[2]周静,王莉,李艳.酸浆果实营养成分分析[J].营养学报,1997,19(2):244-245.
[3]许亮,孙鹏,王冰,杨燕云.酸浆的本草考证[J].中医药学刊,2005,23(5):908-909.
[4]江苏新医学院.中药大辞典(下)[M].上海,上海科学技术出版社,1986:2532-2535.
[5]中华本草编委会.中华本草[M].上海,上海科学技术出版社,第7册.1999:286-290
[6]中国科学院中国植物志编辑委员会.中国植物志[M].北京,科学出版社,第67卷(第1分册).1987:53-59.
[7]Mstsuura T.,Kawai M.,Nakashima R.,Bitter principles of Physalis alkekengi var.franchetii:Structure of physalin A[J].Tetrahedron Lett.,1969,(14):1083-1086.
[8]Mstsuura T.,Kawai M.,Physalis alkekengi var.franchetii:Structure of physalin B[J].Tetrahedron Lett.,1969,(22):1765-1766.
[9]Kawai M.,Mstsuura T.,The structure of physalin C:A bitter principle of Physalis alkekengi var.franchetii[J].Tetrahedron,1970,26(7):1743-1745.
[10]Row Ramachandra L.,Reddy Sambi K.,Sarma Subrahmanya N.,Matsuura T.,Nakashima R.,New physalins form Physalis angulata and Physalis lancifolia.Stucture and reaction of physalins D,I,G and K[J].Phytochemistry,1980,19:1175-1181.
[11]Row Ramachandra L.,Sarma Subrahmanya N.,Matsuura T.,Nakashima R.,Physalins E and H,new physalins form Physalis angulata and Physalis lancifolia[J].Phytochemistry,1978,17:1641-1645.
[12]Row Ramachandra L.,Sarma Subrahmanya N.,Reddy Sambi K.,Matsuura T.,Nakashima R.,The structure of physalins F and J form Physalis angulata and Physalis lancifolia[J].Phytochemistry,1978,17:1647-1650.
[13]Kawai M.,Mstsuura T.,Kyuno S.,Matsuki H.,Takenaka M.,Katsuoka T.,Butsugan Y.,Saito K.,A new physalin from Physalis alkekengi:structure of physalin L[J].Phytochemistry,1987,26(12):3313-3317.
[14]Kawai M.,Ogura T.,Nakanishi M.,Matsuura T.,Butsugan Y.,Mori Y.,Harada K.,Structure of physalin M isolated from Physalis alkekengi var.franchetii[J].Bull.Chem.Soc.Jpn.,1988,61:2696-2698.
[15]Kawai M.,Ogura T.,Makino B.,Matsumoto A.,Yamamura H.,Butsugan Y.,Hayashi M.,Physalin N and O from Physalis alkekengi[J].Phytochernistry,1992,31(12):4299-4302.
[16]Makino B.,Kawai M.,Yasushi I.,Yamamura H.,Butsugan Y.,Ogawa K.,Hayashi M.,Physalins possessing an endoperoxy structure from Physalis alkekengi var.franchetii.Structural revision of physalin K[J].Bull.Chem.Soc.Jpn.,1995,68:219-226.
[17]Makino B.,Kawai M.,Kito K.,Yamamura H.,Butsugan Y.,New physalins possessing an additional carbon-crabon bond from Physalis alkekengi var.franchetii[J].Tetrahedron,1995,51(46):12529-12538.
[18]Makino B.,Kawai M.,Ogura T.,Nakanishi M.,Yamamura H.,Butsugan Y.,Structural revision of physalin H isolated from Physalis angulata[J].Journal of Natural Products,1995,58(11):1668-1674.
[19]Kawai M.,Yamamoto T.,Makino B.,Yamamura H.,Araki S.,Butsuqan Y.,Saito K.,The structure of physalin T from Physalis alkekengi var.franchetii[J].J Asian Nat Prod Res,2001,3(3):199-205.
[20]Sunayama R.,Kuroyanagi M.,Umehara K.,Ueno A.,Physalin and neophysalins from Physalis alkekengi var.franchetii and their differentiation inducing activity[J].Phytochemistry,1993,34(2):529-533.
[21]陈荣,梁敬钰,杨洋,刘睿,酸浆化学成分及酸浆苦素G的结构订正[J].中国天然药物,2007,5(3):186-189.
[22]Ping-Chung Kuo,Tsung-Hsiao Kuo,Amooru G.Damu,Chung-Ren Su,E-Jian Lee,Tian-Shung Wu,Rexen Shu,ChouoMing Chen,Kenneth F.Bastow,Tzu-Hsuan Chen,Kuo-Hsiung Lee,Physanolide A,a novel skeleton steroids,and other cytotoxic principles from Physalis angulata[J].Organic Letters,2006,8(14):2953-2956.
[23]Amooru G.Damu,Ping-Chung Kuo,Chung-Ren Su,Tsung-Hsiao Kuo,Tzu-Hsuan Chen,Kenneth F.Bastow,Kuo-Hsiung Lee,Tian-Shung Wu,Isolation,structure,and structure-cytotoxic activity relationships of withanolides and physalins from Physalis angulata[J].J.Nat.Prod.,2007,(70):1146-1152.
[24]Kawai M.,Ogura T.,Butsugan Y.,Taga T.,Hayashi M.,Benzilic acid rearrangement-type reaction of physalins to neophysalins.Structural revision of one of the dehydration products of physalin A[J].Tetrahedron,1991,47(12/13):2103-2110.
[25]Kawai M.,Matsumoto A.,Makino B.,Mori H.,Ogura T.,Butsugan Y.,Ogawa K.,Hayashi M.,The structure of physalin P,a neophysalin from Physalis alkekengi[J].Bull.Chem.Soc.Jpn.,1993,66:1299-1300.
[26]Rong Chen,Jing-Yu Liang,Rui Liu,Two novel neophysalins from Physalis aIkekengi var.franchetii[J].Helvetica Chimica Acta,2007,90:963-966.[27]Shinya Nagafuji,Hikaru Okabe,Hiroshige Akahane et al.,Trypanocidal constituents in plant withanolides from the aerial parts of Physalis angulata[J].Bio.Pharm.Bull.,2004,27:193-197.
[28]O.E.Vasina,N.D.Abdullaev,N.K.Abubakirov,Withanolides of physalis Ⅸ physangulide-the first natural 22s-withasteroid[J].Chem.Nat.Compd.,1990,26:304-307.
[29]Saeed Ahmad,Abdul Malik,Nighat Afza et al.,A new withanolide glycoside from Physalis peruvina[J].J.Nat.Prod,1990,62:493-494.
[30]G.L.Silva,A.Pacciaroni,J.C.Oberti et al.,A pregnane structurally related to withanolides Physalis viscose[J].Phytochemistry,1993,34:871-873.
[31]Anothony C.Waiss Jr.,Carl A.Euiger,William F.Haddon et al.,Insect inhibitory steroidal saccharide esters from Physalisperuvina[J].J.Nat.Prod.,1993,56:1365-1372.
[32]Kazushi Shingu,Shaji Yahara,Toshihiro Nohara et al.,Three new withanolides,physalin A,B and D from Physalis angulata[J].Chem.Pharm.Bull.,1992,40(8):2088-2091.
[33]Chiu-Ming Chen,Zong-Tsi Chen,Chiu-Hsiang Hsieh et al.,Withangulatin A,a new withanolide from Physalis angulata[J].Heterocycles,1990,31(7):1371 - 1375.
[34]Kazushi Shingu,Shoji Yahara,Hikaru Okabe et al.,Three new withanolides physalins E,F and G from Physalis angulata L.[J].Chem.Pharm.Bull.,1992,40(9):2448-2451.
[35]Kazushi Shingu,Mariko Miyagawa,Shoji Yahara et al.,Physapruins A ans B,two new withanolides from Physalis pruinosa Bailey[J].Chem.Pharm.Bull.,1993,41(10):1873-1875.
[36]Saeed Ahmad,Abdul Malik,Rehana Yasmin,Withanolides from Physalis peruviana[J].Phytochemistry,1999,50:647-651.
[37]Laurence N.Dinah,Satyajit D.Sarker,Vladimir Sik,28-hydroxy withanolide E from Physalis peruviana[J].Phytochemistry,1997,44(3):509-512.
[38]Kazushi Shingu,Nobuhiro Marubayashi,Ikuhiko Veda et al.,Physapruins C,a new withanolide from Physalis angulata L.[J].Chem.Pharm.Bull.,1991,39(6):1591-1593.
[39]Saeed Ahmad,Pehana Yasmin,Abdul Malik,New withanolide glycosides from Physalis perubiana L.[J].Chem.Pharm.Bull.,1999,47(4):477-480.
[40]Emma Maldonado,Fernando R.Torres,Mahinda Matinez et al.,18-acetoxywithanolides from Physalis chenopodifolia[J].Planta Med.,2004,70:59-64.
[41]Perez-Castorena,A.-L.,Oropeza,R.F.,Vazquez,A.R.,Martinez,M.,Maldonado,E.Labdanes and withanolides from Physalis coztomatl[J].J.Nat.Prod.,2006,69(7):1029-1033.
[42]Itoh T.,Butsugan Y.,[J].Phytochemistry,1974,13(10):2239-2241.
[43]N.K.Sharma,D.K.Kulshreshtha,J.S.Yandon et al.,[J].Phytochemistry,1974,13(10):2239-2245.
[44]L.Ramachandta Row,K.Sambi Reddy,N.Subtahsiana Samna et al.,[J].Phytochemistry,1980,19(8):1175-1181.
[45]Itoh T.,Teruo M.,Ruka N.et al.,[J].Phytochemistry,1977,16(11):1723-1728.
[46]李静,李娟,李德坤,锦灯笼化学成分的研究Ⅰ[J].中草药,2002,33(8):692-693.
[47]王明东,杨松松,锦灯笼化学成分及药理作用综述[J].辽宁中医学院学报,2005,7(4):341-342.
[48]梁慧,才谦,锦灯笼果实化学成分的研究[J].中华中医药学刊,2007,25(8):1677-1679.
[49]赵倩,邱莉,卜光明,曲戈霞,邱峰,酸浆宿萼的化学成分[J].沈阳药科大学学报,2006,23(3):151-155.
[50]邱莉,姜志虎,刘红霞,陈丽霞,曲戈霞,邱峰,酸浆宿萼的黄酮苷类化学成分[J].沈阳药科大学学报,2007,24(12):727-730.
[51]马哲.锦灯笼宿萼化学成分的分离与鉴定.辽宁中医药大学硕士毕业论文.2007.5
[52]Ser N.A.,Flavonoids from Physalis minima[J].Phytochemistry,1988,27(11):3708-3709.
[53]N.Ismail,M.Alam,A novel cytotoxic flavonoid glycoside from Physalis angulata[J].Fitoterapia,2001,72:676-679.
[54]Naoki Asano,1β-amino-2α,3β,5β-trihydroxycycloheptane from Physalis alkekengi var.franchetii[J].Phytochemistry,1996,42(3):719-721.
[55]Hideo Y.,Kuniko N.,Studies on the alkaloids of the roots ofPhysalis alkekengi.Isolation of 3α-tigloyloxytropane[J].Chem.Pharm.Bull.,1965,13(2):217-220.
[56]Naoki A.,Atsushi K.,Kengo O.,Haruhisa K.,Katsuhiko M.,Calystrgins of Physalis alkekengi var.franchetii(Solanaceae) Structure determination and their glycosidase inhibitory activities[J].Eur J.Biochem.,1995,229:369-376.
[57]Keith Basey,Brian A.Mcgaw,Jack G.Woolley,Phygrine,an alkaloid from Physalis species[J].Phytochemistry,1992,31(12):4173-4176.
[58]周静,王莉,李艳,鲁建疆,酸浆果实营养成分分析[J].营养学报,1997,19(2):243-245.
[59]吕春平,李娟,吕冬梅,锦灯笼中无机元素含量测定[J].广东微量元素科学,2000,7(6):55-56.
[60]李泽鸿,郝殿明,刘树英,刘洪章,锦灯笼宿萼中营养元素的测定研究[J].世界元素医学,2007,14(4):50-52.
[61]杨晓虹,田丁丁,周小平,酸浆宿萼无机元素和氨基酸含量的测定[J].白求恩医科大学学报,2001,27(1):17-18.
[62]葛玉,段玉峰,刘俊花,肖红,黑龙江酸浆果实及宿萼的成分分析[J].营养学报,2006,28(6):529-532.
[63]蕙伯棣,王亚静,裴凌鹏,李京,张艳,酸浆浆果和宿萼中类胡萝卜素组成分析[J].中国药学杂志2006,41(8):583-585.
[64]韩阳花,酸浆有效成分的初步研究.新疆大学硕士学位论文,2005.
[65]赵倩,董艳丽,曲戈霞,邱峰,酸浆宿萼挥发性成分的研究[J].中药研究与信息,2005,7(4):183-184.
[66]韩阳花,高莉,刘丽艳,酸浆果实中多糖的提取及含量测定[J].食品科学,2006,27(2):154-157.
[67]刘鹏举,薛小刚,马镝,阚国仕,水溶性酸浆果多糖的提取及清除自由基作用的研究[J].食品科技,2007,(12):96-98.
[68]王萍,付勇,苗雨,毛酸浆果实多糖的提取[J].食品工业科技,2008,(2):204-206.
[69]葛玉,段玉峰,刘俊花,刘平,酸浆果多糖不同提取工艺研究[J].食品科学,2006,27(8):178-183.
[70]周静,李艳,酸浆果多糖的提取及含量测定[J].数理医药与杂志,2000,13(3):242-243.
[71]葛玉,段玉峰,刘俊花,张欣,酸浆果萼水溶性多糖提取工艺的比较[J].东北林业大学学报,2006,34(5):73-75.
[72]李娟,李静,锦灯笼化学成分的研究Ⅱ[J].中草药,2002,33(9):788-789.
[73]武海燕,索全伶,李俊,药用植物酸浆的研究进展[J].内蒙古石油化工,2007,(2):5-8.
[74]程瑛琨,李蕾,王丹梅,滕利荣,酸浆宿萼色素的提取工艺及稳定性的研究[J].食品工业科技,2006,27(12):58-62.
[75]甄清,李静,李勇,李文君,李娟,锦灯笼宿萼提物体外抗菌作用研究[J].天然产物研究与开发,2006,(18):273-274.
[76]袁昌衡,周启贵,杨飞中,80种中药水煎液对淋球菌的抑制试验[J].中国药学杂志,1997,17(11):508-509.
[77]宋立人,现代中药学大词典(下册).北京,人民卫生出版社,1999,2311-2314.
[78]李萍,盛巡,王晓中,张建华,鲍朝辉,何延良,丁瑞,酸浆苦素B对活化多形核中性粒细胞化学发光及H_2O_2产生的影响[J].中草药,1996,27(5):284-287.
[79]李萍,盛巡,王晓中,活化嗜中性粒细胞[Ca~(2+)]Ⅰ的变化及锦灯笼酸浆苦素B的影响[J].中国病理生理杂志,1996,12(6):660.
[80]王灿岭,翁何霞,酸浆水提取物的抗炎作用研究[J].中医研究,2007,20(1):17-18.
[81]吕春平,王宏芳,李静,李娟,锦灯笼抗炎作用实验研究[J].现代预防医学,2007,34(12):2213-2215.
[82]李德坤,锦灯笼化学成分的研究.吉林大学硕士学位论文,2001.
[83]吉林省中医中药研究所,长白山植物药志[M].吉林人民出版社1982:100-105.
[84]汪丽燕,木犀草素对冠脉血流动力学的实验研究[J].中国病理学通报,1992,8(5):388-390.
[85]王和平,徐美术,孙亮,宋旭波,锦灯笼降血糖作用的实验研究[J].中医药信息,2004,21(1):53-54.
[86]宋旭波,锦灯笼制剂及其生物活性研究.黑龙江中医药大学硕士学位论文,2003.
[87]李芳莲,范恩学,徐丹,邵静,中药挂金灯的降血脂作用实验研究[J].中国老年学杂志,2006,26(1):91-93.
[88]Antoun MD.,Abramson D.,Tyson RL.,Potential antitumor agents.ⅩⅦ.physalin B and 25,26-epidihydrophysalin C from Witheringia coccoloboides[J].J Nat.Prod.,1981,44:579-585.
[89]Han R.,Recent progress in the study of anti-cancer drugs originating from plants and traditional medicines in China[J].J Chin.Med.Sci.,1994,9:61-69.
[90]Dornberger K.,The potential antineoplastic acting constituents of Physalis alkekengi vat.frenchetii Mast.[J].Pharmazie,1986,41(4):265-268.
[91]Lei Ma,Mumtaz All,Mohammad Arfan,Li-Guang Lou,Li-Hong Hu,Withaphysanolide A,a novel C-27 norwithanolide skeleton,and other cytotoxic compounds from Physalis divericata[J].Tetrahedron Letters,2007,48:449-452.
[92]Lei Ma,Chuan-Ming Xie,Jia Li,Feng-Chang Lou,Li-Hong Hu,Daturametelins H,I,and J:three new withanolide glycosides from Datura metel L.[J].Chemistry & Biodiversity,2006,3:180-186.
[93]Muhammad Iqbal C.,Sammer Y.,Samreen,Syed Adnan Ali S.,Shakil A.,Atta-UR-Rahman,Biotransformation of physalin H and leishrnanicidal activity of its transformed products[J].Chem.Pharm.Bull.,2006,54(7):927-930.
[94]Muhammad lqbal C,,Sammer Y.,Samreen,Syed Adnan Ali S.,Shakil A.,Atta-UR-Rahman,Antileishmanial physalins from Physalis minima[J].Chemistry & Biodiversity,2005,2:1164-1173.
[95]A.H.Januario,E.Rodrigues,R.C.L.R.Pietro,S.Kashima,D.N.Sata,S.C.Franca,Antimycobacterial physalins from Physalis angulata L.(Solanaceae)[J].Phytotherapy Research,2002,16:445-448.
[96]Eloi S.Garcia,Daniele P.Castro,Ivone M.Ribeiro,Therezinha C.B Tomassini,Patricia Azambuja,Trypanosama rangeli:Effects of physalin B on the immune reactions of the infected larvae of Rhodnius prolixus[J].Experimental Parasitology,2006,112:37-43.
[97]Milena B.P.Soares,Moema C.Bellintani,Ivone M.Ribeiro,Therezinha C.B.Tomassini,Ricardo Ribeiro dos Santos,Inhibition of macrophage activation and lipophysaccaride-induced death by seco-steroids purified from Physalis angulata L.[J].European Journal of Pharmacology,2003,459:107-112.
[98]Lei Ma,Xian-Wen Gan,Qing-ping He,Hai-yun Bai,Mohammad Arfan,Feng-chang Lou,Li-hong Hu,Cytotoxic withaphysalins from Physalis minima[J].Helvetica Chimica Acta,2003,90:1406-1419.
[99]M.B.P.Soares,D.Brustolim,L.A.Santos,M.C.Bellintani,F.P.Paiva,Y.M.Ribeiro,T.C.B.Tomassini,R.Ribeiro dos Santo,Physalins B,F,and G,seco-steroids purified from Physalis angulata L.,inhibit lymphocyte function and allogeneic transplant rejection[J].International Immunopharmacology,2006,6:408-414.
[100]Nadia J.Jacobo-Herrera,Paul Bremner,Nieves Marquez,Mahabir P.Gupta,Simon Gibbons,Eduardo Munoz,Michael Heinrich,Physalins from Witheringia solanacea as mudulators of the NF-κB cascade[J].J.Nat.Prod.,2006,69:328-331.
[101]Liqiong Fang,Hee-Byung Chai,Juan J.Castillo,Djaja D.Soejarto,Norman R.Rarnsworth, Geoffrey A.Cordell,John M.Pezzuto,A.Douglas Kinggom,Cytotoxic counstituents of Brachistus stramoniifolius[J].Phytotherapy Research,2003,17:520-523.
[102]龚珊,单立冬,张玉英,郭试瑜,挂金灯镇痛作用的实验观察[J].苏州大学学报(医学版),2002,22(4):380-382.
[103]裴凌鹏,董福慧,孔焕宇,惠伯棣,酸浆提取物改善老龄大鼠抗氧化功能的研究[J].北京中医药大学学报,2007,30(10):688-690.
[104]杨春荣,单静颖,郑素芹,锦灯笼提取物对DPPH自由基清除作用的探讨[J].黑龙江医药科学,2008,31(3):42.
[105]刘寿山,中药文献研究摘要(1920-1961),1963:742.
[106]Vessal M.,Mehrani HA.,Omrani GH.,Effects of an aqueous extract of Physalis alkekengi fruit on estrus cycle,reproduction and uterine creation kinase BB-isozymein rats[J].J.Ethnopharmacol,1991,34(1):69-78.
[107]帕提古丽,马合木提,高莉史博,酸浆花萼色素的提取及理化性质研究[J].食品科学,2004,25(9):35.
[108]王玮,锦灯笼的营养保健功能及药用价值[J].中国食物与营养,2008,(3):54-56.
[109]曹妹芳,刘瑾,张剑华,急咽口服液质量标准研究[J].中成药,1999,21(1):46-47.
[110]杨建刚,清咽利喉方治疗急性咽炎40例[J].吉林中医药,2001,(4):33.
[111]周玲,酸浆果药用食疗方[J].饮食指南,2005,(4):71.
[112]廖木兰,灯笼草外治寻常疣[J].新中医,2004,36(8):74.
[113]郭文扬,酸浆的利用及栽种[J].特种经济动植物,2001,(1):30.
[114]武海燕,索全伶,李俊,药用植物酸浆的研究进展[J].内蒙古石油化工,2007,(2):5-8.
[115]王家东,王荣荣,酸浆开发利用研究的进展[J].农技服务,2007,24(11):109一112.
[116]谭雨清,孟晓敏,刘振扬,红姑娘(挂金灯)原汁饮料加工[J].食品科技,2003,(12):64-66.
[117]王立江,匡明,红姑娘果醋表面发酵法工艺的研究[J].江苏调味副食品,2006,(2):121-125.
[118]张丽,刘奉先,薄层扫描法测定锦灯笼中酸浆果素的含量[J].中医药信息,2000,(5):56.
[119]殷莉梅,韩静,潘岳峰,李智,单娜,锦灯笼含片娇味工艺的研究[J].中国中药杂志,2008,33(13):1635-1637.
[120]杨文菊,敬思群,杨洁,新疆酸浆宿萼总黄酮含量测定方法的研究[J].新疆大学学报(自 然科学版),2007,24(3):339-341.
[121]敬思群,郑力,苏俊,酸浆宿萼总黄酮和多糖的提取及含量测定[J].食品研发与开发,2008,29(6):82-84.
[122]敬思群,阿依努尔,艾合买提江,酸浆宿萼总黄酮提取工艺的研究[J].新疆大学学报,2008,25(1):88-91.
[123]Sen G.,Mulchandani N.B.,Patankar A.V.,Reversed-phase high performance liquid chromatographic separation and analysis of some physalins(13,14-seco- 16,24-cyclo-steroids)[J].Journal of Chromatography,1980,198(2):203-206.
[124]于婷,刘玉强,穆春旭,冯雪,赵红丹,林长旭,才谦,锦灯笼果实中4,7-二脱氢新酸浆苦素B的含量测定[J].辽宇中医杂志,2008,35(4):584-586.
[125]赵红丹,林长旭,尹程鑫,于婷,冯雪,才谦,锦灯笼宿萼醇提物中4,7-二脱氢新酸浆苦素B的含量测定[J].辽宁中医药大学学报,2008,10(4):129-131.
[126]许亮,徐秋阳,王冰,王荣祥,高效液相色谱法测定锦灯笼药材中木犀草素的含量研究[J].时珍国医国药,2006,17(4):575-576.
[127]徐保利,王冰,辽宁各地酸浆宿存萼中木犀草素含量测定[J].山西中医学院学报,2008,10(4):50-52.
[128]王和平,张晓燕,宋旭波,李艳凤,李秋红,HPLC法测定锦灯笼口服液中木犀草素的含量[J].黑龙江医药,2004,17(1):10-12.
[129]许亮,刘红,于荣军,杨燕云,王冰,康廷国,锦灯笼配方颗粒中木犀草素的HPLC 法含量测定[J].中华中医药学刊,2008,26(3):653-654.
[130]施蕊,贾凌云,孙启时,于丹丹,RP-HPLC测定酸浆药材中3种有效成分的含量[J].药物分析杂志,2008,28(2):260-263.
[131]施蕊,贾凌云,孙启时,酸浆药材及其提取物高效液相色谱特征[J].中国中药杂志,2007,32(20):2194-2196.
[132]许亮,王冰,贾天柱,锦灯笼与兔儿伞两种药材的挥发油成分研究[J].中成药,2007,29(12):1840-1843.
[1]Reiko Sunayama,Masanori Kuroyanagi,Kaoru Umehara,Akira Ueno.Physalin and neophysalins from Physalis alkekengi var.francheti and their differentiation inducing activity[J].Phytochemistry,1993,34(2):529-533.
[2]Masao Kawai,Akihide Matsumoto,Bunsho Makino,Hideyuki Mori,Toichi Ogura,Yasuo Butsugan,Kiyoshi Ogawa,Mitsuo Hayashi.The structure of physalin P,a neophysalin from Physalis alkekengi[J].The Chemical Society of Japan,1993,(66):1299-1300.
[3]Liqiong Fang,Hee Byung Chai,Juan J.Castillo,Djaja D.Soejarto,Norman R.Farnsworth,Geoffrey A.Cordell,Johm M.Pezzuto,A.Douglas Kinghorn.Cytotoxic constituents of Brachistus stramoniifolius[J].Phytotherapy Research,2003,(12):520-523.
[4]Masao Kawai,Toichi Ogura,Bunsho Makino,Akihide Matsumoto,Hatsuo Yamamura,Yasuo Butsugan,Mrrsuo Hayashi.Physalins N and O from Physalis alkekengi[J].Phytochemistry,1992,31(12):4299-4320.
[5]Bunsho Makino,Masao Kawai,Toichi Ogura,Masaki Nakanishi,Hatsuo Yamamura,Yasuo Butsugan.Structural revision of physalin H isolated from Physalis angulata[J].Journal of NaturalProducts,1995,58(11):1668-1674.
[6]Masao Kawai,Toichi Ogura,Yasuo Butsugan,Tooru Taga,Mitsuo Hayashi.Benzilic acid rearrangement-type reaction of physlins to neophysalins.Structure revision of one of the dehydroation products of physalin A[J].Tetrahedron,1991,47(12):2103-2110.
[7]L.Ramachandra Row,N.Subrahmanya Sarrna,Teruo Matsuura,Ruka Nakashima.Physalins E and H,new physalins from Physalis angulata and P.lancifolia[J].Phytochemistry,1978,(17):1641-1645.
[8]贾凌云,孙启时,黄顺旺.滁菊花中黄酮类化学成分的分离与鉴定[J].中国药物化学杂志2003,13(3):159-161.
[9]王宇杰,孙启时.金钱草的化学成分研究[J].中国药物化学杂志,2005,15(6):357-359.
[10]周凌云,张祥华,陈昌祥.丽江产红景天的化学成分研究[J].天然产物研院与开发,2004,16(5):410-414.
[11]何桂霞,裴刚,周天达,周雪仙.瑶族藤茶中杨梅素的分离与结构鉴定[J].中国民族医药杂志,2000,6(1):40-42.
[12]Jorge M.David,Frederico G.Cruz,Maria Lenise S.Guedes,Juceni P.Chavez.Flavonol glycosides from Davilla flexuosa[J].J.Braz.Chem.Soc.,1996,7(2):115-118.
[13]梁侨丽.扁豆花化学成分研究[J].中国药科大学学报,1996,27(4):206-207.
[14]邱鹰昆,窦德强,裴玉萍,吉川雅之,松田久司,陈英杰.仙人掌的化学成分研究[J].中国药科大学学报,2005,36(3):213-215.
[15]张思巨,王怡薇,刘丽,于江泳,扈继萍.锁阳化学成分研究Ⅱ[J].中国药学杂志,2007,42(13):974-976.
[16]杨维力,田军,丁立生.白苞裸蒴的化学成分[J].中国中药杂志,2001,26(1):43-45.
[17]赵东保,张卫,李明静,刘绣华.项羽菊化学成分研究[J].中国中药杂志,2006,31(22):1869-1872
[18]Christina Vafiadi,Evangelos Topakas,Ken K.Y.Wong,Ian D.Suckling,Paul Christakopoulos.Mapping the hydrolytic and synthetic selectivity of a type C feruloyl esterase (StFaeC) from Sporotrichum thermophile using alkyl ferulates[J].Tetrahedron:Asymmetry,2005,(16):373-379.
[19]董礼,李磊,廖志华,陈敏,孙敏.柴胡红景天化学成分的研究[J].西北植物学报,2007,27(12):2564-2567.
[20]肖永庆,李丽,游小琳,谷口雅颜.川芎化学成分研究[J].中国中药杂志,2007,27(7):519-521.
[21]章为,徐康平,周应军,谭桂山.两色紫金牛化学成分研究[J].中南药学,2007,5(5):444-448.
[22]姚巍,林文艳,周长新,赵昱.蒙古蒲公英化学成分研究[J].中国中药杂志2007,32(10):926-928.
[23]Kadali S.Sagar,Chia-Chuan Chang,Wei-Kung Wang,Jung-Yaw Lin and Shoei-Sheng Lee,Preparation and anti-HIV activities of retrojusticidin B analogs and azalignans[J].Bioorganic and Medical Chemistry,2004,(12):4045-4054.
[24]古海峰,陈若芸,孙玉华,邢建国.香青兰化学成分研究Ⅱ[J].中国中药杂志,2005,30(9):677-679.
[25]Jesper Jarsen,Dan Staerk,Claus Comett,Steen H.Hansen,Jerzy W.Jaroszewski.Identification of reaction products between drag substances and excipients by HPLC-SPE-NMR:Ester and amide formation between citric acid and 5-aminosalicylic acid[J].Journal of Pharmaceutical and Biomedical Analysis,2009,(49):839-842.
[1]Sen G.,Mulchandani N.B.,Patankar A.V.,Reversed-phase high performance liquid chromatographic separation and analysis of some physalins(13,14-seco-16,24-cyclo-steroids)[J].Journal of Chromatography,1980,198(2):203-206.
[2]于婷,刘玉强,穆春旭,冯雪,赵红丹,林长旭,才谦,锦灯笼果实中4,7-二脱氢新酸浆苦素B的含量测定[J].辽宁中医杂志,2008,35(4):584-586.
[3]赵红丹,林长旭,尹程鑫,于婷,冯雪,才谦,锦灯笼宿萼醇提物中4,7-二脱氢新酸浆苦素B的含量测定[J].辽宇中医药大学学报,2008,10(4):129-131.
[4]许亮,徐秋阳,王冰,王荣祥,高效液相色谱法测定锦灯笼药材中木犀草素的含量研究[J].时珍国医国药.2006,17(4):575-576.
[5]徐保利,王冰,辽宁各地酸浆宿存萼中木犀草素含量测定[J].山西中医学院学报,2008,10(4):50-52.
[6]王和平,张晓燕,宋旭波,李艳凤,李秋红,HPLC法测定锦灯笼口服液中木犀草素的含量[J].黑龙江医药,2004,17(1):10-12.
[7]许亮,刘红,于荣军,杨燕云,王冰,康廷国,锦灯笼配方颗粒中木犀草素的HPLC法含量测定[J].中华中医药学刊,2008,26(3):653-654.
[8]施蕊,贾凌云,孙启时,于丹丹,RP-HPLC测定酸浆药材中3种有效成分的含量[J].药物分析杂志,2008,28(2):260-263.
[9]施蕊,贾凌云,孙启时,酸浆药材及其提取物高效液相色谱特征[J].中国中药杂志,2007,32(20):2194-2196.
[1]Yue X D,Qu G W,Li G S,et al.Stability of salvianolic acid B in water solution[J].Chinese Traditional and Herbal Drugs,2005,36(2):49-51.
[2]Ni L J,Wu K F,Zhang L G.Influence of Heating Method on the Quality of Extracts from Radix Salviae Miltiorrhizae[J].Traditional Chinese Drug Research & Clinical Pharmacology,2006,17(1):56-58.
[3]Lin H B,Lin H K.Study on the stability of Tanshinone ⅡA[J].Traditional Chinese Medicinal Research,2005,18(8):19.
[4]Lu W B,Zeng Y E,Zhong J J,et al.Research on the Stability Factors of Tanshinone Ⅱ_A in Preparation of Salvia miltiorrhiza Bge[J],Lishizhen Medicine and Medica Research,2002,11(1):10-12.
[5]胡春霞,佟凤琴,酸浆果皮色素的提取及稳定性研究[J].辽宁师专学报,2001,3(2):91-93.
[6]李萍,盛巡,王晓中,张建华,鲍朝辉,何延良,丁瑞,酸浆苦素B对活化多形核中性粒细胞化学发光及H_2O_2产生的影响[J].中草药,1996,27(5):284-287.
[7]李萍,盛巡,王晓中,活化嗜中性粒细胞[Ca~(2-)]I的变化及锦灯笼酸浆苦素B的影响[J].中国病理生理杂志,1996,2(6):660.
[8]王灿岭,翁何霞,酸浆水提取物的抗炎作用研究[J].中医研究,2007,20(1):17-18.
[9]吕春平,王宏芳,李静,李娟,锦灯笼抗炎作用实验研究[J].现代预_防医学,2007,34(12):2213-2215.
[10]Fu X C,Shen W X,Yao T Y.Physical chemistry(物理化学),4~(th) Ed.,Beijing,Higher Education Press,1990.
[11]Li X F,Fu J,Jin M Z.Study on degradation dynamics of baicalin in the extraction of Radix Sculellariae by water.Jourmal of Modern Food and Pharmaceticals(现代食品与药品杂志),2006,16(4):32-34.
[12]Xu Z S,Cai B C,Zhang X.Research of the stability of ferulic acid in Rhizoma ChuanXiong.Research and Practice of Chinese Medicines(现代中药研究与实践),2004,18(2):24-26.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |