紫花地丁提取物对耐环丙沙星大肠杆菌耐药性消除作用研究
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摘要
目的:观察中药紫花地丁提取物对大肠杆菌环丙沙星耐药性的消除作用。方法:通过对标准菌株K88~S人工诱导获得两株环丙沙星耐药株,K88~C、K88~Z,并从临床分离的菌株中选取了一多重耐药株RC,以此作为本试验的受试菌株。采用微量棋盘稀释法测定紫花地丁与环丙沙星的联合抑菌浓度指数(FIC);以紫花地丁提取物为消除剂,以SDS作为对照,采用影印培养法,质粒提取及琼脂糖凝胶电泳观察紫花地丁提取物对耐环丙沙星大肠杆菌耐药质粒的消除;观察紫花地丁提取物亚抑菌浓度培养的大肠杆菌对环丙沙星的耐药性消除率;采用荧光测定法测定环丙沙星在细菌体内的蓄积浓度,观察紫花地丁提取物对环丙沙星在菌体内蓄积量的影响。
结果:紫花地丁提取物与环丙沙星联用时对大肠杆菌K88~S,K88~C,K88~Z,RC,所测定FIC指数均为0.5～1,表明二者具有相加作用。紫花地丁提取物亚抑菌浓度作用24h,对耐药菌株K88~C,K88~Z,RC的消除率分别为0.6%、0.8%和1.2%;延长作用至48小时,消除率分别为分别为1.0%、0.8%和1.4%。而对照组SDS(100μg/ml)作用24h,的消除率分别为0.8%、0.8%和1.2%;延长作用至48小时,其消除率可分别提高为1.4%、1.8%和2.0%。经X2检验,两种药物对细菌耐药
Objective:
To study the eliminating effects of herba violae extract on resistance of E. coli to ciprofloxacin
Methods:
Two antibiotic-resistant strains (K88~Cand K88~Z) and a multidrug resistance strain (RC) isolated from E coli K88~S via artificial induction and from young pigs with diarrhea respectively were used as test organisms. The fractional inhibitory concentration (FIC) was detected to evaluate the inhibitory effects of the combination use of herba violae extract and ciprofloxacin on the test organisms above by chessboard dilution method. The methods of replica plating, rapid plasmid extraction and agarose gel electrophoresis were used to observe the eliminating effects of herba violae extract on R plasmid. The accumulative concentration of ciprofloxacin in test organisms treated with herba violae extract was detected by methods of fluorometry.
Results:
结果:紫花地丁提取物与环丙沙星联用时对大肠杆菌K88~S,K88~C,K88~Z,RC,所测定FIC指数均为0.5～1,表明二者具有相加作用。紫花地丁提取物亚抑菌浓度作用24h,对耐药菌株K88~C,K88~Z,RC的消除率分别为0.6%、0.8%和1.2%;延长作用至48小时,消除率分别为分别为1.0%、0.8%和1.4%。而对照组SDS(100μg/ml)作用24h,的消除率分别为0.8%、0.8%和1.2%;延长作用至48小时,其消除率可分别提高为1.4%、1.8%和2.0%。经X2检验,两种药物对细菌耐药
Objective:
To study the eliminating effects of herba violae extract on resistance of E. coli to ciprofloxacin
Methods:
Two antibiotic-resistant strains (K88~Cand K88~Z) and a multidrug resistance strain (RC) isolated from E coli K88~S via artificial induction and from young pigs with diarrhea respectively were used as test organisms. The fractional inhibitory concentration (FIC) was detected to evaluate the inhibitory effects of the combination use of herba violae extract and ciprofloxacin on the test organisms above by chessboard dilution method. The methods of replica plating, rapid plasmid extraction and agarose gel electrophoresis were used to observe the eliminating effects of herba violae extract on R plasmid. The accumulative concentration of ciprofloxacin in test organisms treated with herba violae extract was detected by methods of fluorometry.
Results:
引文
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[1]陆承平.兽医微生物学[M]. 北京:中国农业出版社 2001.215～217
[2]樊丽红,张中直,杨汉春.禽大肠杆菌 DNA 旋转酶活性改变与其对氟喹诺酮类药物耐药性的关系研究[J].畜牧兽医学报,1999,30(1):69
[3]王红宁,刘世贵,刘书亮,等.规模化猪场仔猪白痢的研究[J].中国预防兽医学报,1999,21(4):261
[4]朱力军.动物大肠杆菌耐药性的变化趋势[J].中国兽药杂志,2001,35(2):l6～18
[5]周继红,朱国强,庄国宏.鸡致病性大肠杆菌的分离鉴定.中国畜禽传染病,1997,6(19):27～29
[6]哈尔滨兽医研究所.兽医微生物学[M].北京:中国农业科学院,1997.235～237
[7]倪语星,洪秀华. 细菌耐药性监测与抗感染治疗[M]. 北京:人民军医出版社,2002.47～85
[8]曹澍泽.兽医微生物学及免疫学技术[M].北京: 北京农业技术出版社,1992.58～63
[9]Serrano FJ,Munoz RM,Vidal SS,et al. Epidemiological aspects of ciprofloxaine resistant Escherichia coli at a general hospital[J]. Rev Esp Quimioter. 2000,13(1):44～50.
[10]陈孝进,彭 兰.5 年临床常见病原菌对环丙沙星耐药趋势[J].中华医院感染学杂志.2006, 16(2):216
[11]张健骈,李跃龙,陈鲜花,等.禽致病性大肠杆菌对氟喹诺酮类药物交叉耐药性研究[J].广东农业科学,2005,(2):74-76
[12]陈群.黄芩与止痢灵对大肠杆菌 R 质粒消除作用的研究[J].中国微生态学杂志,1998, 10(2):80-82
[13]康梅.三黄片对大肠杆菌耐药质粒消除作用的研究[J].华西药学杂志,l999,l4(5～ 6):406～408
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[1]陆承平.兽医微生物学[M]. 北京:中国农业出版社 2001.215～217
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[4]朱力军.动物大肠杆菌耐药性的变化趋势[J].中国兽药杂志,2001,35(2):l6～18
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[10]陈孝进,彭 兰.5 年临床常见病原菌对环丙沙星耐药趋势[J].中华医院感染学杂志.2006, 16(2):216
[11]张健骈,李跃龙,陈鲜花,等.禽致病性大肠杆菌对氟喹诺酮类药物交叉耐药性研究[J].广东农业科学,2005,(2):74-76
[12]陈群.黄芩与止痢灵对大肠杆菌 R 质粒消除作用的研究[J].中国微生态学杂志,1998, 10(2):80-82
[13]康梅.三黄片对大肠杆菌耐药质粒消除作用的研究[J].华西药学杂志,l999,l4(5～ 6):406～408
[14]邓旭明,于录,阎继业.外输泵抑制剂对喹诺酮抗凝固酶阴性葡萄球菌活性的影响[J].广州医药,2002,33(3):49～51
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