铁及其络合物对银屑病治疗作用实验研究
摘要
【目的】
本研究利用雌激素周期小鼠阴道上皮细胞有丝分裂和鼠尾鳞片表皮颗粒层形成两种银屑病的动物实验模型,探讨铁及络合铁对银屑病的治疗作用。
【方法】
1.铁及络合铁对雌激素周期小鼠模型阴道上皮细胞有丝分裂的影响
使用昆明种雌性小鼠建立雌激素周期小鼠阴道上皮细胞有丝分裂模型,随机分为阴性对照组、阳性对照组,FeCl_3低、中、高剂量组,Fe-EDTA低、中、高剂量组,朴雪口服液组。光学显微镜下观察阴道上皮细胞有丝分裂情况,计算有丝分裂指数。
2.铁及络合铁对鼠尾鳞状表皮模型颗粒层形成的影响
将实验小鼠随机分为9组(同上)。光学显微镜下观察鼠尾鳞片表皮颗粒层形成情况,计数100个鳞片中有颗粒层的鳞片数。
3.模型小鼠组织微量元素含量的测定
取各组小鼠的肝、脾、肾、尾组织经微波消解仪消解后用原子吸收分光光度计分析小鼠各组织中的铜、铁、锌、钙、镁、锰含量。
4.对模型小鼠肝、脾、肾组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)的影响
用试剂盒测定各组小鼠肝、脾、肾组织匀浆中SOD、GSH-Px的活力和MDA含量。
5.对模型小鼠血清IL-2和IL-10含量的影响
用放射免疫法测定各组小鼠血清中IL-2和IL-10含量。
【结果】
1.铁及络合铁对雌激素周期小鼠模型阴道上皮细胞有丝分裂的影响
与阴性对照组相比,各补铁组和阳性对照组均显著降低,各补铁组与阳性对照组比较,差异无统计学意义(P＞0.05),提示各补铁组对小鼠阴道上皮细胞有丝分裂有显著的抑制作用,其效果与MTX相似(P＜0.01)。除阴性对照组外,其他各组进行两两比较,均无统计学差异(P＞0.05)。
2.铁及络合铁对鼠尾鳞状表皮模型颗粒层形成的影响
各补铁组和阳性对照组与阴性对照组相比均显著升高(P＜0.01),各补铁组与阳性对照组无统计学差异(P＞0.05),提示各补铁组对鼠尾上皮鳞片中颗粒层形成有显著的促进作用,效果与MTX相似。除阴性对照组外,其他各组进行两两比较,均无统计学差异(P＞0.05)。
3.模型小鼠组织微量元素含量的测定
脾组织中各补铁组及阳性对照组的铁含量均高于阴性对照组(P＜0.05),阳性对照组与各补铁组比较,差异无统计学意义(P＞0.05)。在肝、肾、尾组织中,各组铁含量两两比较均无统计学意义(P＞0.05)。
肝、脾、肾、尾组织中,各组Cu、Fe、Mg、Mn、Ca5种元素含量分别两两比较均无统计学差异(P＞0.05)。
4.对模型小鼠肝、脾、肾中SOD、GSH-Px和MDA的影响
肝组织中,各补铁组SOD活力及GSH-Px活力降低,MDA含量升高,但差异无统计学意义(P＞0.05)。脾组织中,各补铁组SOD活力,MDA含量升高,但差异无统计学意义(P＞0.05)。肾组织中,各组之间两两比较,SOD活力、MDA含量及GSH-Px活力均无统计学差异(P＞0.05)。
5.对模型小鼠血清IL-2和IL-10含量的影响
阳性对照组和各补铁组血清中的IL-2含量高于阴性对照组,但差异无统计学意义(P＞0.05),各补铁组与阳性对照组比较无统计学意义(P＞0.05)。补铁组间两两比较均无统计学意义(P＞0.05)。
阴性对照组、各补铁组血清中的IL-10含量高于阳性对照组,但是无统计义意义(P＞0.05)。各补铁组间两两比较均无统计学意义(P＞0.05)。
【结论】
1.补铁后雌激素周期模型小鼠阴道上皮细胞有丝分裂受到显著抑制,鼠尾鳞片颗粒层形成显著促进。
2.补铁后只是脾铁含量显著升高,对必需元素铜、铁、锌、镁、锰和钙无明显促排作用。
3.补铁可能影响抗氧化体系,提高机体应激水平。
4.补铁对血清IL-2水平,IL-10水平无明显影响。
Objective
The effect of iron and iron complex on inhibiting psoriasis development in mouse vaginal epithelium model and mouse tail scale epidermis model were explored.
Methods
1.Effects of iron and iron complex on the vaginal epithelium mitosis of estrone periodical mice model.
Female kunming mice were used to establish the epidermis hyperplasia of estrone periodical mice model.They were randomly divided into nine groups: negative control,positive control,low,middle,high dosage of FeCl_3,low,middle, high dosage of Fe-EDTA and iron lactate.The mice were sacrificed by cervical dislocation after the last administration,and the vaginal mitosis index was observed under optical microscope.
2.Effects on mouse mouse-tail scale epidermis models
Mice used as the epidermis parakeratosis model were randomly divided into nine groups indicated as above.The formation of mouse-tail scale epidermis was observed and the numbers of scale with granular layer per 100 scales were examined by optical microscope.
3.Analysis of essential elements.
To investigate concentrations of copper,iron,zinc,calcium,magnesium, manganum and selenium,liver,spleen,kidney and tail of mice in all groups were taken and digested to analyze the elements concentration by atomic absorption spectrophotometry.
4.Effects on recovering the anti-oxidizing system
The activities of SOD,GSH-PX and the content of MDA in the homogenate of liver,kidney and spleen of the mice were determined in each group.
5.Effects on the serum level of IL-2 and IL-10
Radioimmunoassay was applied to measure the serum level of IL-2 and IL-10 in each group.
Results
1.Effects of zinc and zinc complex on mouse vaginal epithelium model
Every iron spiked groups as well as methotrexate group could obviously inhibit the mitosis of vaginal epithelial cells(P＜0.01).No statistical significance was found between each iron spiked group and the positive control group(P＞0.05).
2.Effects on mouse tail scale epidermis models
There was statistical significance between each iron spiked group as well as the positive control and the negative control group(P＞0.05).
3.Effects on removing other essential elements
Iron contents of each iron spiked group and methotrexate group in spleen were higher than that of the negative control group(P＜0.05).No statistical significance was found between the iron spiked group and methotrexate group(P＞0.05).In comparison with iron contents,no statistical significance existed among the groups in the liver, kidney and tail(P＞0.05).
In comparison with copper,zinc,magnesium,manganum and calcium contents, no statistical significance existed among the groups in the liver,spleen,kidney and tail(P＞0.05).
4.Effects on recovering the anti-oxidizing system
In comparison with the corresponding data of the negative control group,the activities of SOD decreased while the contents of MDA increased.However,no statistical significance existed in the livers,kidneys and spleen.
5.Effects on the serum level of IL-2 and IL-10
The serum level of IL-2 in each iron spiked group as well as methotrexate group was higher than that in the negative control group,but no statistical significance existed(P＞0.05).
The serum level of IL-10 increased in each iron spiked group,but there was no statistical significance(P＞0.05).
Conclusion
1.As a result of iron replenishment,the mitosis of vaginal epithelial cells was obviously·inhibited,and the formation of granular layer in mice tail scale was significantly promoted.
2.Iron replenishment could obviously enhance iron contents in spleen and had no obvious effect on other essential elements such as copper,zinc,magnesium, manganum,and calcium.
3.It could also enhance the activity of the antioxidant enzyme.
4.There were no obvious effects on the serum level of IL-2 and IL-10.
本研究利用雌激素周期小鼠阴道上皮细胞有丝分裂和鼠尾鳞片表皮颗粒层形成两种银屑病的动物实验模型,探讨铁及络合铁对银屑病的治疗作用。
【方法】
1.铁及络合铁对雌激素周期小鼠模型阴道上皮细胞有丝分裂的影响
使用昆明种雌性小鼠建立雌激素周期小鼠阴道上皮细胞有丝分裂模型,随机分为阴性对照组、阳性对照组,FeCl_3低、中、高剂量组,Fe-EDTA低、中、高剂量组,朴雪口服液组。光学显微镜下观察阴道上皮细胞有丝分裂情况,计算有丝分裂指数。
2.铁及络合铁对鼠尾鳞状表皮模型颗粒层形成的影响
将实验小鼠随机分为9组(同上)。光学显微镜下观察鼠尾鳞片表皮颗粒层形成情况,计数100个鳞片中有颗粒层的鳞片数。
3.模型小鼠组织微量元素含量的测定
取各组小鼠的肝、脾、肾、尾组织经微波消解仪消解后用原子吸收分光光度计分析小鼠各组织中的铜、铁、锌、钙、镁、锰含量。
4.对模型小鼠肝、脾、肾组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)的影响
用试剂盒测定各组小鼠肝、脾、肾组织匀浆中SOD、GSH-Px的活力和MDA含量。
5.对模型小鼠血清IL-2和IL-10含量的影响
用放射免疫法测定各组小鼠血清中IL-2和IL-10含量。
【结果】
1.铁及络合铁对雌激素周期小鼠模型阴道上皮细胞有丝分裂的影响
与阴性对照组相比,各补铁组和阳性对照组均显著降低,各补铁组与阳性对照组比较,差异无统计学意义(P＞0.05),提示各补铁组对小鼠阴道上皮细胞有丝分裂有显著的抑制作用,其效果与MTX相似(P＜0.01)。除阴性对照组外,其他各组进行两两比较,均无统计学差异(P＞0.05)。
2.铁及络合铁对鼠尾鳞状表皮模型颗粒层形成的影响
各补铁组和阳性对照组与阴性对照组相比均显著升高(P＜0.01),各补铁组与阳性对照组无统计学差异(P＞0.05),提示各补铁组对鼠尾上皮鳞片中颗粒层形成有显著的促进作用,效果与MTX相似。除阴性对照组外,其他各组进行两两比较,均无统计学差异(P＞0.05)。
3.模型小鼠组织微量元素含量的测定
脾组织中各补铁组及阳性对照组的铁含量均高于阴性对照组(P＜0.05),阳性对照组与各补铁组比较,差异无统计学意义(P＞0.05)。在肝、肾、尾组织中,各组铁含量两两比较均无统计学意义(P＞0.05)。
肝、脾、肾、尾组织中,各组Cu、Fe、Mg、Mn、Ca5种元素含量分别两两比较均无统计学差异(P＞0.05)。
4.对模型小鼠肝、脾、肾中SOD、GSH-Px和MDA的影响
肝组织中,各补铁组SOD活力及GSH-Px活力降低,MDA含量升高,但差异无统计学意义(P＞0.05)。脾组织中,各补铁组SOD活力,MDA含量升高,但差异无统计学意义(P＞0.05)。肾组织中,各组之间两两比较,SOD活力、MDA含量及GSH-Px活力均无统计学差异(P＞0.05)。
5.对模型小鼠血清IL-2和IL-10含量的影响
阳性对照组和各补铁组血清中的IL-2含量高于阴性对照组,但差异无统计学意义(P＞0.05),各补铁组与阳性对照组比较无统计学意义(P＞0.05)。补铁组间两两比较均无统计学意义(P＞0.05)。
阴性对照组、各补铁组血清中的IL-10含量高于阳性对照组,但是无统计义意义(P＞0.05)。各补铁组间两两比较均无统计学意义(P＞0.05)。
【结论】
1.补铁后雌激素周期模型小鼠阴道上皮细胞有丝分裂受到显著抑制,鼠尾鳞片颗粒层形成显著促进。
2.补铁后只是脾铁含量显著升高,对必需元素铜、铁、锌、镁、锰和钙无明显促排作用。
3.补铁可能影响抗氧化体系,提高机体应激水平。
4.补铁对血清IL-2水平,IL-10水平无明显影响。
Objective
The effect of iron and iron complex on inhibiting psoriasis development in mouse vaginal epithelium model and mouse tail scale epidermis model were explored.
Methods
1.Effects of iron and iron complex on the vaginal epithelium mitosis of estrone periodical mice model.
Female kunming mice were used to establish the epidermis hyperplasia of estrone periodical mice model.They were randomly divided into nine groups: negative control,positive control,low,middle,high dosage of FeCl_3,low,middle, high dosage of Fe-EDTA and iron lactate.The mice were sacrificed by cervical dislocation after the last administration,and the vaginal mitosis index was observed under optical microscope.
2.Effects on mouse mouse-tail scale epidermis models
Mice used as the epidermis parakeratosis model were randomly divided into nine groups indicated as above.The formation of mouse-tail scale epidermis was observed and the numbers of scale with granular layer per 100 scales were examined by optical microscope.
3.Analysis of essential elements.
To investigate concentrations of copper,iron,zinc,calcium,magnesium, manganum and selenium,liver,spleen,kidney and tail of mice in all groups were taken and digested to analyze the elements concentration by atomic absorption spectrophotometry.
4.Effects on recovering the anti-oxidizing system
The activities of SOD,GSH-PX and the content of MDA in the homogenate of liver,kidney and spleen of the mice were determined in each group.
5.Effects on the serum level of IL-2 and IL-10
Radioimmunoassay was applied to measure the serum level of IL-2 and IL-10 in each group.
Results
1.Effects of zinc and zinc complex on mouse vaginal epithelium model
Every iron spiked groups as well as methotrexate group could obviously inhibit the mitosis of vaginal epithelial cells(P＜0.01).No statistical significance was found between each iron spiked group and the positive control group(P＞0.05).
2.Effects on mouse tail scale epidermis models
There was statistical significance between each iron spiked group as well as the positive control and the negative control group(P＞0.05).
3.Effects on removing other essential elements
Iron contents of each iron spiked group and methotrexate group in spleen were higher than that of the negative control group(P＜0.05).No statistical significance was found between the iron spiked group and methotrexate group(P＞0.05).In comparison with iron contents,no statistical significance existed among the groups in the liver, kidney and tail(P＞0.05).
In comparison with copper,zinc,magnesium,manganum and calcium contents, no statistical significance existed among the groups in the liver,spleen,kidney and tail(P＞0.05).
4.Effects on recovering the anti-oxidizing system
In comparison with the corresponding data of the negative control group,the activities of SOD decreased while the contents of MDA increased.However,no statistical significance existed in the livers,kidneys and spleen.
5.Effects on the serum level of IL-2 and IL-10
The serum level of IL-2 in each iron spiked group as well as methotrexate group was higher than that in the negative control group,but no statistical significance existed(P＞0.05).
The serum level of IL-10 increased in each iron spiked group,but there was no statistical significance(P＞0.05).
Conclusion
1.As a result of iron replenishment,the mitosis of vaginal epithelial cells was obviously·inhibited,and the formation of granular layer in mice tail scale was significantly promoted.
2.Iron replenishment could obviously enhance iron contents in spleen and had no obvious effect on other essential elements such as copper,zinc,magnesium, manganum,and calcium.
3.It could also enhance the activity of the antioxidant enzyme.
4.There were no obvious effects on the serum level of IL-2 and IL-10.
引文
[1]Henseler T.The genetics of psoriasis[J].J Am Acad Dermatol,1997,37:S1-11
[2]欧阳恒,杨志波主编.银屑病的诊断与治疗[M].北京:人民军医出版社,2001.1-2;20;104-111
[3]Oguz Savran F,Diler A,Erzengin D et al.HLA-DRB1 association in Turkish Psoriasis vulgaris patients[J].Swiss Med Wldy,2003,133(39-40):541-543
[4]周宗立,王懿娜,方红.银屑病生物治疗研究进展[J].浙江中西医结合杂志,2003,13(10):659-661
[5]Storbeek K,Holzle E,Schurer N et al.Narrow band(31 lnm)versus conventional broad band UVB with and without dithranol in phototherapy for psoriasis[J].J Am Acad Dermatol,1993,28:227
[6]窦海中,郝平生,姜青松.银屑病临床治疗中的点滴体会[J].中医杂志,2003,44(2):101
[7]Laburte C R,Grossman J,Abi-Roched J.Efficacy and safety of oral cyclosporine A for long-term treatment of chronic severe plaque psoriasis[J].Br J Dermatol,1994,130:366
[8]Culliffe W J,Berth-Jones J,Claudy A et al.Comparative study of calcipotriol ointment and betamethason-17 valerate in patients with psoriasis vulgaris[J].J Am Acad Dermatol,1992,26:736
[9]张雪梅.银屑病治疗进展[J].内蒙古民族大学学报(自然科学版),2004,19(1):92-95
[10]于敬红.银屑病硒水平与病程和病情的关系[J].国外医学医学地理分册,2003,24(4):168-172
[11]王立平,张丽娜,董兵.银屑病及真菌性皮肤病的发病治疗机理初探.微量元素与健康,Studies of Trace Elements and Health.2005,22(1):10-19
[12]涂伟欣.银屑病患者头发中微量元素的测定及研究[J].微量元素与健康研究,1996,13(2):34
[13]李博镏.皮肤病防治358问[M].北京:中国中医药出版社,1998
[14]Van Scot EJ,Bonder RH.Intravaginal and intrarectal screening of antimitotic drugs for topical effectiveness[J].J Invest Dermatol,1971,56(2):132
[15]Jarret A.The physiology and pathophysiology of the skin[J].Lancet.1973, 2(7826):445
[16]孙林潮,刘玉峰.银屑病动物模型研究进展[J].国外医学皮肤性病学分册,1995,21(5):264-267
[17]曹伟,袁玮.海洋生物酸性多糖的抗银屑病作用及对免疫功能的影响[J].中国药理学通报,1997,13(2):160-163
[18]孙爱军,王荣,贾正平.银屑病的药物治疗,第四军医大学学报[J].Fourth Mil Med Univ,2007,28(21):2014-2015
[19]李素芬,罗绪刚,刘彬等.有机微量元素对幼虫的效应及作用机理研究进展.中国畜牧杂志[J].2001,2:51-53
[20]周利梅等.微量元素铁在动物营养中的研究进展.畜禽业[J].2000,12:12-14
[21]孔繁增,周向昭,王建,郜贺荣,张桂梅,安国芝,孟昭影,陈守斌.银屑病人群微量元素营养状况的临床流行病学分析.微量元素与健康研究,2001,18(4):20-22
[22]曹家琪,崔荣,张忠祥,徐汉卿,冯捷.银屑病患者血清微量元素的测定,China J Lepr Skin Dis,2005,21(8):617-619
[23]孙克会,周子亮,王显义.痤疮等五种常见皮肤病患发中微量元素分析[J].微量元素与健康研究,2004(1):12-13.
[24]丁政云,孙笑菊,高顺强等.银屑病患者血清微量元素的测定[J].临床皮肤科杂志,2003,32(9):525.
[25]梁东东,刘子文,张让.寻常型银屑病患者发内微量元素的测定[J].中华皮肤科杂志,1999,32(2):107.
[26]徐伟钦,董承超.银屑病患者头发中微量元素的测定研究[J].微量元素与健康研究,1996,13(2):34-44.
[27]江文,陈兴平.吡啶硫铁气雾剂治疗寻常型银屑病临床疗效评价[J].Central China Medical Journel,2003,27(2):60-62
[28]付军,崔晞,刘振军,刘娜,丁勃,管益涛.锌及络合锌抗银屑病作用的实验研究.山东大学学报(医学版),2006,44(12):1206-1208
[29]B.J.Wang,N.Liu,W.Hu,J.Fu,D.M.Guo,S.E.Wang and X.Cui.The effects of ZnCl2 and Zn-EDTA on the development of psoriasis.Trace elements and electrolytes,2008,25(2):55-59
[30]胡薇,马赛,郭冬梅,季静,杨晓倩,崔晞.亚硒酸钠与维生素C联合应用对 银屑病模型抑制作用的研究.山东大学学报(医学版),2007,45(12):1214-1219
[31]曹国华,陈吉棣,刘小鹏等.缺铁及补铁对鼠脂质过氧化与超氧化物歧化酶的影响[J].中华医学杂志,1991,71(11):623
[32]彭立义,张道杰,叶明亮等.银屑病患者抗氧化酶、脂质过氧化物及肿瘤坏死因子测定的意义[J].前卫医药杂志,1997,14(5):340-341
[33]党倩丽,吕俊霞,李长海.氧自由基在银屑病皮损区作用的研究[J].陕西医学杂志,2002,31(9):791-793
[2]欧阳恒,杨志波主编.银屑病的诊断与治疗[M].北京:人民军医出版社,2001.1-2;20;104-111
[3]Oguz Savran F,Diler A,Erzengin D et al.HLA-DRB1 association in Turkish Psoriasis vulgaris patients[J].Swiss Med Wldy,2003,133(39-40):541-543
[4]周宗立,王懿娜,方红.银屑病生物治疗研究进展[J].浙江中西医结合杂志,2003,13(10):659-661
[5]Storbeek K,Holzle E,Schurer N et al.Narrow band(31 lnm)versus conventional broad band UVB with and without dithranol in phototherapy for psoriasis[J].J Am Acad Dermatol,1993,28:227
[6]窦海中,郝平生,姜青松.银屑病临床治疗中的点滴体会[J].中医杂志,2003,44(2):101
[7]Laburte C R,Grossman J,Abi-Roched J.Efficacy and safety of oral cyclosporine A for long-term treatment of chronic severe plaque psoriasis[J].Br J Dermatol,1994,130:366
[8]Culliffe W J,Berth-Jones J,Claudy A et al.Comparative study of calcipotriol ointment and betamethason-17 valerate in patients with psoriasis vulgaris[J].J Am Acad Dermatol,1992,26:736
[9]张雪梅.银屑病治疗进展[J].内蒙古民族大学学报(自然科学版),2004,19(1):92-95
[10]于敬红.银屑病硒水平与病程和病情的关系[J].国外医学医学地理分册,2003,24(4):168-172
[11]王立平,张丽娜,董兵.银屑病及真菌性皮肤病的发病治疗机理初探.微量元素与健康,Studies of Trace Elements and Health.2005,22(1):10-19
[12]涂伟欣.银屑病患者头发中微量元素的测定及研究[J].微量元素与健康研究,1996,13(2):34
[13]李博镏.皮肤病防治358问[M].北京:中国中医药出版社,1998
[14]Van Scot EJ,Bonder RH.Intravaginal and intrarectal screening of antimitotic drugs for topical effectiveness[J].J Invest Dermatol,1971,56(2):132
[15]Jarret A.The physiology and pathophysiology of the skin[J].Lancet.1973, 2(7826):445
[16]孙林潮,刘玉峰.银屑病动物模型研究进展[J].国外医学皮肤性病学分册,1995,21(5):264-267
[17]曹伟,袁玮.海洋生物酸性多糖的抗银屑病作用及对免疫功能的影响[J].中国药理学通报,1997,13(2):160-163
[18]孙爱军,王荣,贾正平.银屑病的药物治疗,第四军医大学学报[J].Fourth Mil Med Univ,2007,28(21):2014-2015
[19]李素芬,罗绪刚,刘彬等.有机微量元素对幼虫的效应及作用机理研究进展.中国畜牧杂志[J].2001,2:51-53
[20]周利梅等.微量元素铁在动物营养中的研究进展.畜禽业[J].2000,12:12-14
[21]孔繁增,周向昭,王建,郜贺荣,张桂梅,安国芝,孟昭影,陈守斌.银屑病人群微量元素营养状况的临床流行病学分析.微量元素与健康研究,2001,18(4):20-22
[22]曹家琪,崔荣,张忠祥,徐汉卿,冯捷.银屑病患者血清微量元素的测定,China J Lepr Skin Dis,2005,21(8):617-619
[23]孙克会,周子亮,王显义.痤疮等五种常见皮肤病患发中微量元素分析[J].微量元素与健康研究,2004(1):12-13.
[24]丁政云,孙笑菊,高顺强等.银屑病患者血清微量元素的测定[J].临床皮肤科杂志,2003,32(9):525.
[25]梁东东,刘子文,张让.寻常型银屑病患者发内微量元素的测定[J].中华皮肤科杂志,1999,32(2):107.
[26]徐伟钦,董承超.银屑病患者头发中微量元素的测定研究[J].微量元素与健康研究,1996,13(2):34-44.
[27]江文,陈兴平.吡啶硫铁气雾剂治疗寻常型银屑病临床疗效评价[J].Central China Medical Journel,2003,27(2):60-62
[28]付军,崔晞,刘振军,刘娜,丁勃,管益涛.锌及络合锌抗银屑病作用的实验研究.山东大学学报(医学版),2006,44(12):1206-1208
[29]B.J.Wang,N.Liu,W.Hu,J.Fu,D.M.Guo,S.E.Wang and X.Cui.The effects of ZnCl2 and Zn-EDTA on the development of psoriasis.Trace elements and electrolytes,2008,25(2):55-59
[30]胡薇,马赛,郭冬梅,季静,杨晓倩,崔晞.亚硒酸钠与维生素C联合应用对 银屑病模型抑制作用的研究.山东大学学报(医学版),2007,45(12):1214-1219
[31]曹国华,陈吉棣,刘小鹏等.缺铁及补铁对鼠脂质过氧化与超氧化物歧化酶的影响[J].中华医学杂志,1991,71(11):623
[32]彭立义,张道杰,叶明亮等.银屑病患者抗氧化酶、脂质过氧化物及肿瘤坏死因子测定的意义[J].前卫医药杂志,1997,14(5):340-341
[33]党倩丽,吕俊霞,李长海.氧自由基在银屑病皮损区作用的研究[J].陕西医学杂志,2002,31(9):791-793