Purification and identification of a novel antimicrobial protein from the rabbit sacculus rotundus and its effect on cellular immune function in mice
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- 英文论文题名：Purification and identification of a novel antimicrobial protein from the rabbit sacculus rotundus and its effect on cellular immune function in mice
- 论文作者：Chen hongwei ; Li yinglun ; Wu junwei ; Liu juan ; Huang qingzhou ; Wei shuyong ; Zhao guangwei ; Zhang zhiqiang ; Hua ling
- 英文论文作者：Chen hongwei ; Li yinglun ; Wu junwei ; Liu juan ; Huang qingzhou ; Wei shuyong ; Zhao guangwei ; Zhang zhiqiang ; Hua ling ; Department of Veterinary Medicine ; Rongchang Campus ; Southwest University ; College of Veterinary Medicine ; Sichuan Agricultural University
- 年：2016
- 作者机构：Department of Veterinary Medicine,Rongchang Campus,Southwest University;College of Veterinary Medicine,Sichuan Agricultural University;
- 英文论文关键词：sacculus rotundus ; antimicrobial protein ; NanoLC-ESI-MS/MS ; cytokines ; lymphocyte proliferation
- 会议召开时间：2016-05-25
- 会议录名称：第七届中国畜牧科技论坛论文集
- 英文会议录名称：Proceeding of the 7th China Animal Husbandry Sci-tech Forum
- 语种：英文
- 分类号：S852.3
- 学会代码：ZGXJ
- 会议名称：第七届中国畜牧科技论坛
- 会议地点：中国重庆
- 主办单位：中国畜牧兽医学会
- 学会名称：中国畜牧兽医学会
- 页数：10
- 文件大小：2189k
- 原文格式：O
- 会议级别：全国
摘要
The aim of this study was to identify theantimicrobial substances in the sacculus rotundus and evaluate their effects on mammalian immune function and molecular mechanisms.Chromatographic techniques were used to purify antimicrobial substances.Components with antimicrobial activity were analyzed by NanoLC-ESI-MS/MS.Immunosuppressed mice were given crude protein extract or purified antimicrobial protein by gavage for 4 weeks,and serum cytokine levels were measured.Splenic lymphocyte proliferation assays were also performed.We obtained histone cluster 1,H2 bcTike,which had antimicrobial activity.We termed this protein rabbit sacculus rotundus antimicrobial histone(RSRAH),according to its primary source.Sequence analysis and alignment suggested that EKTPVKKKARKSAGAAKRKA was a core segment of the protein mediating its antimicrobial activity.Treatment with high doses of the crude protein extract for 14,21,and 28 days significantly increased interferon-γ(IFN-γ) levels compared to the control.Interleukin(IL)-4 levels were also increased significantly on days 7,14,21,and 28 in the experimental group.Moreover,treatment with the crude protein extract stimulated a significant increase in lymphocyte proliferation on days 14 and 28.Administration of antimicrobial protein isolated from the rabbit sacculus rotundus to immunosuppressed mice increased serum IFN-γ and IL-4 levels and induced lymphocyte proliferation,thereby promoting cellular immune function in immunosuppressed mice.
The aim of this study was to identify theantimicrobial substances in the sacculus rotundus and evaluate their effects on mammalian immune function and molecular mechanisms.Chromatographic techniques were used to purify antimicrobial substances.Components with antimicrobial activity were analyzed by NanoLC-ESI-MS/MS.Immunosuppressed mice were given crude protein extract or purified antimicrobial protein by gavage for 4 weeks,and serum cytokine levels were measured.Splenic lymphocyte proliferation assays were also performed.We obtained histone cluster 1,H2 bcTike,which had antimicrobial activity.We termed this protein rabbit sacculus rotundus antimicrobial histone(RSRAH),according to its primary source.Sequence analysis and alignment suggested that EKTPVKKKARKSAGAAKRKA was a core segment of the protein mediating its antimicrobial activity.Treatment with high doses of the crude protein extract for 14,21,and 28 days significantly increased interferon-γ(IFN-γ) levels compared to the control.Interleukin(IL)-4 levels were also increased significantly on days 7,14,21,and 28 in the experimental group.Moreover,treatment with the crude protein extract stimulated a significant increase in lymphocyte proliferation on days 14 and 28.Administration of antimicrobial protein isolated from the rabbit sacculus rotundus to immunosuppressed mice increased serum IFN-γ and IL-4 levels and induced lymphocyte proliferation,thereby promoting cellular immune function in immunosuppressed mice.
The aim of this study was to identify theantimicrobial substances in the sacculus rotundus and evaluate their effects on mammalian immune function and molecular mechanisms.Chromatographic techniques were used to purify antimicrobial substances.Components with antimicrobial activity were analyzed by NanoLC-ESI-MS/MS.Immunosuppressed mice were given crude protein extract or purified antimicrobial protein by gavage for 4 weeks,and serum cytokine levels were measured.Splenic lymphocyte proliferation assays were also performed.We obtained histone cluster 1,H2 bcTike,which had antimicrobial activity.We termed this protein rabbit sacculus rotundus antimicrobial histone(RSRAH),according to its primary source.Sequence analysis and alignment suggested that EKTPVKKKARKSAGAAKRKA was a core segment of the protein mediating its antimicrobial activity.Treatment with high doses of the crude protein extract for 14,21,and 28 days significantly increased interferon-γ(IFN-γ) levels compared to the control.Interleukin(IL)-4 levels were also increased significantly on days 7,14,21,and 28 in the experimental group.Moreover,treatment with the crude protein extract stimulated a significant increase in lymphocyte proliferation on days 14 and 28.Administration of antimicrobial protein isolated from the rabbit sacculus rotundus to immunosuppressed mice increased serum IFN-γ and IL-4 levels and induced lymphocyte proliferation,thereby promoting cellular immune function in immunosuppressed mice.
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