A Genome-Scale Analysis of the PIN Gene Family Reveals Its Functions in Cotton Fiber Development
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- 英文论文题名：A Genome-Scale Analysis of the PIN Gene Family Reveals Its Functions in Cotton Fiber Development
- 论文作者：Zhang Yuzhou ; He Peng ; Yang Zuoren ; Huang Gai ; Wang Limin ; Pang Chaoyou ; Xiao Hui ; Zhao Peng ; Yu Jianing ; Xiao Guanghui
- 英文论文作者：Zhang Yuzhou ; He Peng ; Yang Zuoren ; Huang Gai ; Wang Limin ; Pang Chaoyou ; Xiao Hui ; Zhao Peng ; Yu Jianing ; Xiao Guanghui ; College of Life Sciences ; Shaanxi Normal University ; Institute of Cotton Research of Chinese Academy of Agricultural Sciences ; Peking University
- 年：2017
- 作者机构：College of Life Sciences,Shaanxi Normal University;Institute of Cotton Research of Chinese Academy of Agricultural Sciences;Peking University;
- 英文论文关键词：cotton ; PIN-formed ; auxin efflux carriers ; fiber development ; expression patterns
- 会议召开时间：2017-08-07
- 会议录名称：中国农学会棉花分会2017年年会暨第九次会员代表大会论文汇编
- 语种：英文
- 分类号：S562
- 学会代码：MHXB
- 会议名称：中国农学会棉花分会2017年年会暨第九次会员代表大会
- 会议地点：中国河南郑州
- 主办单位：中国农学会棉花分会
- 学会名称：《棉花学报》编辑部
- 页数：1
- 文件大小：42k
- 原文格式：O
- 会议级别：全国
摘要
The PIN-FORMED(PIN) protein,the most important polar auxin transporter,plays a critical role in the distribution of auxin and controls multiple biological processes.However,characterizations and functions of this gene family have not been identified in cotton.Here,we identified the PIN gene family in Gossypium hirsutum,G.arboreum and G.raimondii.This gene family was divided into seven subgroups.A chromosomal distribution analysis showed that GhPIN genes were evenly distributed in eight chromosomes and that the whole genome and dispersed duplications were the main duplication events for GhPIN expansion.qRT-PCR analysis showed a tissue-specific expression pattern for GhPIN.Likely due to the cis-element variations in their promoters,transcripts of PIN6 and PIN8 genes from the At(tetraploid genome orginated from G.arboreum) subgenome and PIN1 a from the Dt(tetraploid genome orginated from G.raimondii) subgenome in G.hirsutum was significantly increased compared with the transcripts in the diploids.The differential regulation of these PIN genes after the polyploidization may be conductive to fiber initiation and elongation.Exogenously applied auxin polar transport inhibitor significantly suppressed fiber growth,which is consistent with the essential function of these PIN genes in regulating cotton fiber development.Furthermore,the overexpression of GhPIN1a_Dt,GhPIN6_At,and GhPIN8_At in Arabidopsis promoted the density and length of trichomes in leaves.
The PIN-FORMED(PIN) protein,the most important polar auxin transporter,plays a critical role in the distribution of auxin and controls multiple biological processes.However,characterizations and functions of this gene family have not been identified in cotton.Here,we identified the PIN gene family in Gossypium hirsutum,G.arboreum and G.raimondii.This gene family was divided into seven subgroups.A chromosomal distribution analysis showed that GhPIN genes were evenly distributed in eight chromosomes and that the whole genome and dispersed duplications were the main duplication events for GhPIN expansion.qRT-PCR analysis showed a tissue-specific expression pattern for GhPIN.Likely due to the cis-element variations in their promoters,transcripts of PIN6 and PIN8 genes from the At(tetraploid genome orginated from G.arboreum) subgenome and PIN1 a from the Dt(tetraploid genome orginated from G.raimondii) subgenome in G.hirsutum was significantly increased compared with the transcripts in the diploids.The differential regulation of these PIN genes after the polyploidization may be conductive to fiber initiation and elongation.Exogenously applied auxin polar transport inhibitor significantly suppressed fiber growth,which is consistent with the essential function of these PIN genes in regulating cotton fiber development.Furthermore,the overexpression of GhPIN1a_Dt,GhPIN6_At,and GhPIN8_At in Arabidopsis promoted the density and length of trichomes in leaves.
The PIN-FORMED(PIN) protein,the most important polar auxin transporter,plays a critical role in the distribution of auxin and controls multiple biological processes.However,characterizations and functions of this gene family have not been identified in cotton.Here,we identified the PIN gene family in Gossypium hirsutum,G.arboreum and G.raimondii.This gene family was divided into seven subgroups.A chromosomal distribution analysis showed that GhPIN genes were evenly distributed in eight chromosomes and that the whole genome and dispersed duplications were the main duplication events for GhPIN expansion.qRT-PCR analysis showed a tissue-specific expression pattern for GhPIN.Likely due to the cis-element variations in their promoters,transcripts of PIN6 and PIN8 genes from the At(tetraploid genome orginated from G.arboreum) subgenome and PIN1 a from the Dt(tetraploid genome orginated from G.raimondii) subgenome in G.hirsutum was significantly increased compared with the transcripts in the diploids.The differential regulation of these PIN genes after the polyploidization may be conductive to fiber initiation and elongation.Exogenously applied auxin polar transport inhibitor significantly suppressed fiber growth,which is consistent with the essential function of these PIN genes in regulating cotton fiber development.Furthermore,the overexpression of GhPIN1a_Dt,GhPIN6_At,and GhPIN8_At in Arabidopsis promoted the density and length of trichomes in leaves.
引文