柴胡疏肝散水提取物在大鼠外翻肠囊实验中的吸收研究
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- 英文论文题名：Investigation of Absorption Characteristics of Extracts from Chaihu-Shu-Gan-San(CSGS)in Rat Everted Gut Scas
- 论文作者：刘洋 ; 贾红梅 ; 于猛 ; 邹忠梅
- 英文论文作者：LIU Yang ; JIA Hong-mei ; YU Meng ; ZOU Zhong-mei ; Tianjin University of Traditional Chinese Medicine ; Institute of Medicinal Plant Development ; Chinese Academy of Medical Sciences and Peking Union Medical College
- 年：2016
- 作者机构：天津中医药大学;中国医学科学院北京协和医学院药用植物研究所;
- 论文关键词：柴胡疏肝散水提取物 ; 外翻肠囊法 ; UPLC ; 累计吸收量 ; 吸收速率常数
- 英文论文关键词：decoction extraction processes from Chaihu-Shu-Gan-San ; everted intestinal sac method ; UPLC ; cumulative amount ; absorption rate constant
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R285.5
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：4
- 文件大小：275k
- 原文格式：O
- 会议级别：全国
摘要
目的:通过建立大鼠外翻肠囊实验模型,考察柴胡舒肝散水提物中6种指标性成分体外肠吸收特征。方法:采用大鼠外翻肠囊实验模型,通过建立UPLC定量分析方法测定不同时间点十二指肠、空肠、回肠中芍药苷、阿魏酸、芸香柚皮苷、柚皮苷、新橙皮苷、甘草酸的肠道吸收量,计算吸收动力学参数,考察上述成分在不同肠段的吸收特征。结果:各肠段累积吸收量(Q)结果显示,除甘草酸外,其他5种成分在各肠段的累积吸收量均具有明显差异;肠吸收速率(Ka)计算结果显示:芍药苷的吸收速率空肠>十二指肠>回肠;阿魏酸、芸香柚皮苷、新橙皮苷的吸收速率十二指肠>空肠>回肠;柚皮苷吸收速率空肠>回肠>十二指肠;甘草酸的吸收速率在空肠吸收好于十二指肠和回肠,但无显著差异。结论:肠囊对柴胡舒肝散水提物中6种指标性成份吸收具有选择性,各成分在不同肠道中的吸收均为线性吸收,其相关系数的平方(R~2)均达到0.9以上,符合零级吸收速率。
OBJECTIVE The research aimed to evaluate the intestinal absorption of extracted by decoction extraction processes from Chaihu-ShuGan-San via everted gut sacs.To investigate the influence of active compounds of Chaihu-Shu-Gan-San(CSGS)decoction extraction on the intestinal absorption of paeoniflorin,ferulic acid,narirutin,naringin,neohesperidin and glycyrrhizic acid,respectively.METHODS:Taking rat everted gut sac model,intestinal sac contents were collected at different time and absorption concentration of the six components above was determined by UPLC,absorption kinetics parameters were calculated for describing their absorption characters in different intestinal segments(duodenum,jejunum and ileum).RESULTS:Absorption of glycyrrhizic acid in duodenum,jejunum and ileum was no significant difference,while the other five components showed large differences;The absorption rate(Ka)show that:the absorption rate of paeoniflorin was jejunum>duodenum>ileum;the absorption rate of ferulic acid,narirutin and neohesperidin was duodenum>jejunum>ileum;the absorption rate of naringin was jejunum>ileum>duodenum;the absorption rate of glycyrrhizic acid in jejunum was better than the other two intestinal segments,while there was no significant difference.CONCLUSION:The absorption of six components of decoction extraction processes from Chaihu-Shu-Gan-San had choices,every component was linearity in different intestine,and the square of coefficient correlation exceed 0.9,which consistent with zero order rate process.
OBJECTIVE The research aimed to evaluate the intestinal absorption of extracted by decoction extraction processes from Chaihu-ShuGan-San via everted gut sacs.To investigate the influence of active compounds of Chaihu-Shu-Gan-San(CSGS)decoction extraction on the intestinal absorption of paeoniflorin,ferulic acid,narirutin,naringin,neohesperidin and glycyrrhizic acid,respectively.METHODS:Taking rat everted gut sac model,intestinal sac contents were collected at different time and absorption concentration of the six components above was determined by UPLC,absorption kinetics parameters were calculated for describing their absorption characters in different intestinal segments(duodenum,jejunum and ileum).RESULTS:Absorption of glycyrrhizic acid in duodenum,jejunum and ileum was no significant difference,while the other five components showed large differences;The absorption rate(Ka)show that:the absorption rate of paeoniflorin was jejunum>duodenum>ileum;the absorption rate of ferulic acid,narirutin and neohesperidin was duodenum>jejunum>ileum;the absorption rate of naringin was jejunum>ileum>duodenum;the absorption rate of glycyrrhizic acid in jejunum was better than the other two intestinal segments,while there was no significant difference.CONCLUSION:The absorption of six components of decoction extraction processes from Chaihu-Shu-Gan-San had choices,every component was linearity in different intestine,and the square of coefficient correlation exceed 0.9,which consistent with zero order rate process.
引文
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[2]王术玲,贾薇,高晓玲.柴胡疏肝散水提液HPLC特征图谱研究及指标成分的测定[J].中成药,2012,12:2384-2388.
[3]陈煜辉,周莺.柴胡疏肝散抗抑郁作用的药理学实验研究[J].江西中医学院学报,2004,04:59-60.
[4]李云辉,张春虎,王素娥,邱娟,胡随瑜,肖桂林.柴胡疏肝散对慢性应激抑郁模型大鼠行为及血浆促肾上腺皮质激素释放激素和促肾上腺皮质激素的影响[J].中西医结合学报,2009,11:1073-1077.
[5]尚立芝,季书,王琦,潘晓丽.柴胡疏肝散的抗肝纤维化作用研究[J].中药药理与临床,2014,05:8-11.
[6]邱新建.柴胡疏肝散促胃肠动力成分的药效学和药物代谢动力学研究[D].中南大学,2011.
[7]陈泣,龚飞鹏,刘兆华,钟凌云,龚千锋.柳穿鱼叶苷和柳穿鱼黄素的大鼠肠外翻吸收研究[J].世界科学技术-中医药现代化,2014,02:352-357.
[8]李娜,陈西敬,吴磊,卢杨,何焱,苏云明.甘草酸对芍药苷大鼠肠吸收的影响[J].时珍国医国药,2008,12:2908-2909.
[9]刘姗姗,李凌军,徐继航.四妙勇安汤中绿原酸、甘草苷、阿魏酸在大鼠离体小肠的吸收动力学研究[J].中南药学,2015,04:369-372.
[10]杨星钢,张立波,潘卫三,张建援.芍药苷大鼠在体肠吸收动力学的研究[J].中国药学杂志,2006,11:854-857.