Toxic effects of phoxim on rats and the protective role of vitamin E
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- 英文论文题名：Toxic effects of phoxim on rats and the protective role of vitamin E
- 论文作者：Jing Zhang ; Wentao Song ; Yuecheng Sun ; Anshan Shan
- 英文论文作者：Jing Zhang ; Wentao Song ; Yuecheng Sun ; Anshan Shan ; Institute of Animal Nutrition ; Northeast Agricultural University
- 年：2016
- 作者机构：Institute of Animal Nutrition,Northeast Agricultural University;
- 英文论文关键词：Phoxim ; Vitamin E ; SD rats ; Toxic effect ; Protective Role
- 会议召开时间：2016-10-21
- 会议录名称：中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集
- 语种：英文
- 分类号：S859.79
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
- 会议地点：中国湖北武汉
- 主办单位：中国畜牧兽医学会动物营养学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：78k
- 原文格式：O
- 会议级别：全国
摘要
Phoxim,a typical organophosphorus pesticide(OP),is a broad-spectrum insecticide for the control of lepidopteran pests,and widely used in agriculture,domestic and veterinary in China.The increased use of phoxim,seen as less of a threat to the environment due to the high effectiveness and low persistence.Chronic exposure to small amounts of pesticide leads to deleterious effects on metabolism,neuronal stimulation,hormonal secretion,and etc.Several organs such as pancreas,liver,muscles,kidney and brain can be affected by pesticide.Some nutrients have modifying effect on the toxicity of contaminants.Interactions between nutrients and contaminants can enhance the protection against negative effects of unwanted.For example,vitamin E(tocopherols),flavonoids,and fatty acids amend the toxicity of pesticide.Vitamin E(a-tocopherol) is thought to be an important chain breaking antioxidant and it prevents the production of lipid peroxides by scavenging free radicals which are toxic byproducts of many metabolic processes in biological membranes.The protective effect of vitamin E on pesticide induced oxidative stress has been reported.The experiment was conducted to evaluate the toxic effects of phoxim on rats and the protective role of vitamin E.A single factor design was adopted,a total of 48 SD rats were divided into 4 treatments randomly(each treatment represented by 3 replicates of 4 rats),including 3 experimental treatments:phoxim(180 mg/kg BW),vitamin E(200 mg/kg BW),phoxim(180 mg/kg BW)+vitamin E(200 mg/kg BW) and control group.The phoxim was administered orally which dissolve in soybean oil.The dosing volume was 5mL.During the experiment period,the rats were given sterile food and water ad libitum.The Sprague-Dawley rats were acclimated for a period of one week.Rats were kept in plastic rat cages,and had free access to food and water.Weigh SD rats' weight and record at approximately 8:30 a.m.on each day,calculate phoxim requirements.The study commenced at the beginning of the 42-day-old and lasted for 30 days.The results showed as follows:1) Compare with the control group,exposure to low concentration of phoxim lead to the injuries of SD rat,such as decreased the average daily gain(ADG),average daily feed intake(ADFI) and body weight significantly(P<0.01).2)Compared with the control group,the content of GGT,ALP,ROS in serum increased(P<0.05),while CHE,BUN,CR reduced significantly(P<0.05).3)Phoxim triggered oxidative stress,which is mainly reflected in altered the content of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)significantly(P<0.01)and malondialdehyde(MDA)(P<0.05).4)In addition,the results indicated that phoxim affected the inflammatory cytokines,genes expression of detoxification enzymes,which play important roles in organophosphorus pesticide resistance,and injury in the liver and kidney of SD rat.5)On the other hand,the results demonstrated that added vitamin E in pesticide group modified the phoxim toxicity in rat,such as increased the ADG,ADFI and the expression of detoxification enzymes(CYP450)(P<0.05).Vitamin E decreased the phoxim-induced oxidative stress,the content of MDA,CAT decreased significantly(P<0.05),while the content of GSH,SOD increased(P<0.05).Compared with the phoxim(180 mg/kgBW)group,increased the content of GLB in serum increased(P<0.05)and ALT,BUN reduced significantly(P<0.05)in group 3(phoxim+vitamin E).In conclusion,the results indicated that vitamin E modified the phoxim-induced toxicity in rat.
Phoxim,a typical organophosphorus pesticide(OP),is a broad-spectrum insecticide for the control of lepidopteran pests,and widely used in agriculture,domestic and veterinary in China.The increased use of phoxim,seen as less of a threat to the environment due to the high effectiveness and low persistence.Chronic exposure to small amounts of pesticide leads to deleterious effects on metabolism,neuronal stimulation,hormonal secretion,and etc.Several organs such as pancreas,liver,muscles,kidney and brain can be affected by pesticide.Some nutrients have modifying effect on the toxicity of contaminants.Interactions between nutrients and contaminants can enhance the protection against negative effects of unwanted.For example,vitamin E(tocopherols),flavonoids,and fatty acids amend the toxicity of pesticide.Vitamin E(a-tocopherol) is thought to be an important chain breaking antioxidant and it prevents the production of lipid peroxides by scavenging free radicals which are toxic byproducts of many metabolic processes in biological membranes.The protective effect of vitamin E on pesticide induced oxidative stress has been reported.The experiment was conducted to evaluate the toxic effects of phoxim on rats and the protective role of vitamin E.A single factor design was adopted,a total of 48 SD rats were divided into 4 treatments randomly(each treatment represented by 3 replicates of 4 rats),including 3 experimental treatments:phoxim(180 mg/kg BW),vitamin E(200 mg/kg BW),phoxim(180 mg/kg BW)+vitamin E(200 mg/kg BW) and control group.The phoxim was administered orally which dissolve in soybean oil.The dosing volume was 5mL.During the experiment period,the rats were given sterile food and water ad libitum.The Sprague-Dawley rats were acclimated for a period of one week.Rats were kept in plastic rat cages,and had free access to food and water.Weigh SD rats' weight and record at approximately 8:30 a.m.on each day,calculate phoxim requirements.The study commenced at the beginning of the 42-day-old and lasted for 30 days.The results showed as follows:1) Compare with the control group,exposure to low concentration of phoxim lead to the injuries of SD rat,such as decreased the average daily gain(ADG),average daily feed intake(ADFI) and body weight significantly(P<0.01).2)Compared with the control group,the content of GGT,ALP,ROS in serum increased(P<0.05),while CHE,BUN,CR reduced significantly(P<0.05).3)Phoxim triggered oxidative stress,which is mainly reflected in altered the content of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)significantly(P<0.01)and malondialdehyde(MDA)(P<0.05).4)In addition,the results indicated that phoxim affected the inflammatory cytokines,genes expression of detoxification enzymes,which play important roles in organophosphorus pesticide resistance,and injury in the liver and kidney of SD rat.5)On the other hand,the results demonstrated that added vitamin E in pesticide group modified the phoxim toxicity in rat,such as increased the ADG,ADFI and the expression of detoxification enzymes(CYP450)(P<0.05).Vitamin E decreased the phoxim-induced oxidative stress,the content of MDA,CAT decreased significantly(P<0.05),while the content of GSH,SOD increased(P<0.05).Compared with the phoxim(180 mg/kgBW)group,increased the content of GLB in serum increased(P<0.05)and ALT,BUN reduced significantly(P<0.05)in group 3(phoxim+vitamin E).In conclusion,the results indicated that vitamin E modified the phoxim-induced toxicity in rat.
Phoxim,a typical organophosphorus pesticide(OP),is a broad-spectrum insecticide for the control of lepidopteran pests,and widely used in agriculture,domestic and veterinary in China.The increased use of phoxim,seen as less of a threat to the environment due to the high effectiveness and low persistence.Chronic exposure to small amounts of pesticide leads to deleterious effects on metabolism,neuronal stimulation,hormonal secretion,and etc.Several organs such as pancreas,liver,muscles,kidney and brain can be affected by pesticide.Some nutrients have modifying effect on the toxicity of contaminants.Interactions between nutrients and contaminants can enhance the protection against negative effects of unwanted.For example,vitamin E(tocopherols),flavonoids,and fatty acids amend the toxicity of pesticide.Vitamin E(a-tocopherol) is thought to be an important chain breaking antioxidant and it prevents the production of lipid peroxides by scavenging free radicals which are toxic byproducts of many metabolic processes in biological membranes.The protective effect of vitamin E on pesticide induced oxidative stress has been reported.The experiment was conducted to evaluate the toxic effects of phoxim on rats and the protective role of vitamin E.A single factor design was adopted,a total of 48 SD rats were divided into 4 treatments randomly(each treatment represented by 3 replicates of 4 rats),including 3 experimental treatments:phoxim(180 mg/kg BW),vitamin E(200 mg/kg BW),phoxim(180 mg/kg BW)+vitamin E(200 mg/kg BW) and control group.The phoxim was administered orally which dissolve in soybean oil.The dosing volume was 5mL.During the experiment period,the rats were given sterile food and water ad libitum.The Sprague-Dawley rats were acclimated for a period of one week.Rats were kept in plastic rat cages,and had free access to food and water.Weigh SD rats' weight and record at approximately 8:30 a.m.on each day,calculate phoxim requirements.The study commenced at the beginning of the 42-day-old and lasted for 30 days.The results showed as follows:1) Compare with the control group,exposure to low concentration of phoxim lead to the injuries of SD rat,such as decreased the average daily gain(ADG),average daily feed intake(ADFI) and body weight significantly(P<0.01).2)Compared with the control group,the content of GGT,ALP,ROS in serum increased(P<0.05),while CHE,BUN,CR reduced significantly(P<0.05).3)Phoxim triggered oxidative stress,which is mainly reflected in altered the content of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)significantly(P<0.01)and malondialdehyde(MDA)(P<0.05).4)In addition,the results indicated that phoxim affected the inflammatory cytokines,genes expression of detoxification enzymes,which play important roles in organophosphorus pesticide resistance,and injury in the liver and kidney of SD rat.5)On the other hand,the results demonstrated that added vitamin E in pesticide group modified the phoxim toxicity in rat,such as increased the ADG,ADFI and the expression of detoxification enzymes(CYP450)(P<0.05).Vitamin E decreased the phoxim-induced oxidative stress,the content of MDA,CAT decreased significantly(P<0.05),while the content of GSH,SOD increased(P<0.05).Compared with the phoxim(180 mg/kgBW)group,increased the content of GLB in serum increased(P<0.05)and ALT,BUN reduced significantly(P<0.05)in group 3(phoxim+vitamin E).In conclusion,the results indicated that vitamin E modified the phoxim-induced toxicity in rat.
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