冷胁迫下二倍体野生种瑟伯氏棉和三裂稀转录组比较分析（英文）
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- 英文论文题名：Comparative Analysis of Two Different Cotton Diploid Wild Species,Gossypium thurberi and Gossypium trilobum,under Cold Stress by RNA-Seq
- 论文作者：蔡小彦 ; 许艳超 ; 周忠丽 ; 王星星 ; 王玉红 ; 张振梅 ; 刘方 ; 王坤波
- 英文论文作者：Cai Xiaoyan ; Xu Yanchao ; Zhou Zhongli ; Wang Xingxing ; Wang Yuhong ; Zhang Zhenmei ; Liu Fang ; Wang Kunbo ; State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences
- 年：2017
- 作者机构：棉花生物学国家重点实验室/中国农业科学院棉花研究所;
- 英文论文关键词：Gossypium thurberi ; G.trilobum ; cold stress ; diploid wild cotton ; RNA-Seq ; CBF
- 会议召开时间：2017-08-07
- 会议录名称：中国农学会棉花分会2017年年会暨第九次会员代表大会论文汇编
- 语种：英文
- 分类号：S562
- 学会代码：MHXB
- 会议名称：中国农学会棉花分会2017年年会暨第九次会员代表大会
- 会议地点：中国河南郑州
- 主办单位：中国农学会棉花分会
- 学会名称：《棉花学报》编辑部
- 页数：1
- 文件大小：47k
- 原文格式：O
- 会议级别：全国
摘要
Cotton(Gossypium)is one of the important fiber crops of the world.Cold stress affects the nor-mal growth of cotton in every period.Although it is a complicated mechanism to response cold stress,RNA-Seq technology provides a new strategy to reveal that.The diploid species Gossypium thurberi and Gossypium trilobum exposed to cold stress(4℃)were used to study the cotton cold tolerance mechanisms by the next-generation RNA-Seq technology.Phenotypic and physiological analysis proved that G.thurberi was better at regulating to adapt cold stress than G.trilobum.We mapped RNA-Seq data to reference genome(G.raimondii),then 23 726 and 23 820 genes had been found in G.thurberi and G.trilobum,severally.What is more,there are 4227 and 4174 differentially expressed genes(DEGs)were found in G.thurberi and G.trilobum,respectively.Furthermore,we found signal transduction,transcription factors,and carbohydrate transport and metabolism play important roles in responding to cold stress by DEGs enrichment analysis.And,in this study,we found that G.thurberi accumulated higher levels ofabscisic acid(ABA)than G.trilobum.ABA-mediated CBF4 signal network have been predicted by our data.ICE2,CBF4,RAP2.7,key genes of ABA biosynthesis,that belong to CBF4 regulated network,were identified and analyzed.And,these genes expression were up-regulated because of cold stress.These data suggest that ABA mediated CBF4 regulated network also play a core role in cotton.Therefore,this study not only provides materias and a basic theory for cotton cold tolerance mechanism,but also a new idea to study abiotic stress deeply.
Cotton(Gossypium) is one of the important fiber crops of the world.Cold stress affects the normal growth of cotton in every period.Although it is a complicated mechanism to response cold stress,RNA-Seq technology provides a new strategy to reveal that.The diploid species Gossypium thurberi and Gossypium trilobum exposed to cold stress(4 ℃) were used to study the cotton cold tolerance mechanisms by the next-generation RNA-Seq technology.Phenotypic and physiological analysis proved that G.thurberi was better at regulating to adapt cold stress than G.trilobum.We mapped RNA-Seq data to reference genome(G.raimondii),then 23 726 and 23 820 genes had been found in G.thurberi and G.trilobum,severally.What is more,there are 4227 and 4174 differentially expressed genes(DEGs) were found in G.thurberi and G.trilobum,respectively.Furthermore,we found signal transduction,transcription factors,and carbohydrate transport and metabolism play important roles in responding to cold stress by DEGs enrichment analysis.And,in this study,we found that G.thurberi accumulated higher levels of abscisic acid(ABA) than G.trilobum.ABA-mediated CBF4 signal network have been predicted by our data.ICE2,CBF4,RAP2.7,key genes of ABA biosynthesis,that belong to CBF4 regulated network,were identified and analyzed.And,these genes expression were up-regulated because of cold stress.These data suggest that ABA mediated CBF4 regulated network also play a core role in cotton.Therefore,this study not only provides materias and a basic theory for cotton cold tolerance mechanism,but also a new idea to study abiotic stress deeply.
Cotton(Gossypium) is one of the important fiber crops of the world.Cold stress affects the normal growth of cotton in every period.Although it is a complicated mechanism to response cold stress,RNA-Seq technology provides a new strategy to reveal that.The diploid species Gossypium thurberi and Gossypium trilobum exposed to cold stress(4 ℃) were used to study the cotton cold tolerance mechanisms by the next-generation RNA-Seq technology.Phenotypic and physiological analysis proved that G.thurberi was better at regulating to adapt cold stress than G.trilobum.We mapped RNA-Seq data to reference genome(G.raimondii),then 23 726 and 23 820 genes had been found in G.thurberi and G.trilobum,severally.What is more,there are 4227 and 4174 differentially expressed genes(DEGs) were found in G.thurberi and G.trilobum,respectively.Furthermore,we found signal transduction,transcription factors,and carbohydrate transport and metabolism play important roles in responding to cold stress by DEGs enrichment analysis.And,in this study,we found that G.thurberi accumulated higher levels of abscisic acid(ABA) than G.trilobum.ABA-mediated CBF4 signal network have been predicted by our data.ICE2,CBF4,RAP2.7,key genes of ABA biosynthesis,that belong to CBF4 regulated network,were identified and analyzed.And,these genes expression were up-regulated because of cold stress.These data suggest that ABA mediated CBF4 regulated network also play a core role in cotton.Therefore,this study not only provides materias and a basic theory for cotton cold tolerance mechanism,but also a new idea to study abiotic stress deeply.
引文