乌司他丁治疗改善重症中暑后肺泡表面活性物质的减少
详细信息 查看官网全文
- 英文论文题名：The curative effect of ulinastatin on the decrease of pulmonary surfactant in the rats with acute lung injury induced by severe heat-stroke
- 论文作者：陈玉兰 ; 林幼萍 ; 罗家劲 ; 江东新 ; 陈怿
- 英文论文作者：CHEN Yulan ; LIN Youping ; LUO Jiajing ; JIANG Dongxin ; CHEN Yi ; Department of Critical Care Medicine ; The Fifth People's Hospital of Dongguan/Dongguan Hospital Affiliated to Medical College of Jinan University ; The medical college of Shantou university
- 年：2017
- 作者机构：东莞市第五人民医院/暨南大学医学院附属东莞医院重症医学科;汕头大学医学院;
- 论文关键词：重症中暑 ; 急性肺损伤 ; 乌司他丁 ; 肺泡表面活性蛋白B ; 磷脂
- 英文论文关键词：severe heatstroke ; acute lung injury ; ulinastatin ; pulmonary surfactant protein B ; phospholipid
- 会议召开时间：2017-05-26
- 会议录名称：2017年《中国组织化学与细胞化学杂志》临床研讨会论文集
- 语种：中文
- 分类号：R594.12
- 学会代码：ZHXB
- 会议名称：2017年《中国组织化学与细胞化学杂志》临床研讨会
- 会议地点：中国湖北武汉
- 主办单位：《中国组织化学与细胞化学杂志》社有限公司
- 学会名称：《中国组织化学与细胞化学杂志》社有限公司
- 页数：2
- 文件大小：243k
- 原文格式：O
- 会议级别：全国
摘要
目的:观察乌司他丁对重症中暑急性肺损伤大鼠肺泡表面活性物质蛋白B(SP-B)和磷脂浓度的影响。方法:将60只)健康SPF级成年雄性SD大鼠建立重症中暑急性肺损伤模型,建模成功后随机分为研究1组、研究2组、研究3组,每组20只动物,分别给予研究1组大鼠尾静脉注射高剂量乌司他丁(20万U/kg),给予研究2组大鼠尾静脉注射低剂量乌司他丁(5万U/kg),给予研究3组大鼠注射等剂量生理盐水,分别于腹腔注射药物前及第30min、1h、2h每组各分批次处死5只大鼠,采用酶联免疫吸附法(ELISA)法检测肺组织中SP-B的表达水平,使用比色法测定支气管肺泡灌洗液中磷脂成分浓度。结果:注药前3组大鼠肺组织SP-B和磷脂浓度均无显著差异(P>0.05),注药后30min、1h、2h,研究1组大鼠肺组织SP-B和磷脂浓度均明显高于研究2组(P<0.05)和研究3组(P<0.01),研究2组大鼠肺组织中的SP-B和磷脂浓度亦高于研究3组大鼠(P<0.05)。结论:乌司他丁能够有效的延缓重症中暑大鼠模型肺组织中SP-B和磷脂肺泡表面活性物质的减少,对重症中暑所致急性肺损伤具有保护效果。
Objective to investigate the effect of ulinastatin on the expression of pulmonary surfactant protein B(SP-B) and phospholipid in the rats with acute lung injury induced by severe heat-stroke.Methods:Sixty healthy adult male SD rats were used to stimulate acute lung injury with severe heat-stroke.The animals were randomly divided into three groups after onset of heat-stroke(n = 20 in each group).High dose ulinastatin(200000 U/kg) were intravenously injected via tail vein in Group 1.Low dose ulinastatin(50000 U/kg) were intravenously injected in Group 2.Equal volume normal saline were injected intravenously in Group 3.The expression of SP-B in the lung tissue was mesured by enzyme-linked immunosorbent assay(ELISA) and the concentration of phospholipid in bronchoalveolar lavage fluid(BALF) was detected by colorimetry in five sacrificed rodents of every group before administration as well as 30 min,1h and 2h after administration.Results:The level of SP-B and phospholipid had no significant difference among the three groups before administration.(P > 0.05).The concentrations of SP-B and phospholipid in Group 1 were significantly higher than Group 2(P < 0.05) and group 3(P < 0.01) at 30 min,1 h and 2h after ulinastatin treatment Further,the production of SP-B and phospholipid in Group 2 were also more than Group 3 at the same time.(P < 0.05).Conclusions:Ulinastatin has a curative effect on acute lung injury caused by severe heat-stroke.These effect may be related to improve the decrease of SP-B and phospholipid in lung tissue of severe heat-stroke rats effectively
Objective to investigate the effect of ulinastatin on the expression of pulmonary surfactant protein B(SP-B) and phospholipid in the rats with acute lung injury induced by severe heat-stroke.Methods:Sixty healthy adult male SD rats were used to stimulate acute lung injury with severe heat-stroke.The animals were randomly divided into three groups after onset of heat-stroke(n = 20 in each group).High dose ulinastatin(200000 U/kg) were intravenously injected via tail vein in Group 1.Low dose ulinastatin(50000 U/kg) were intravenously injected in Group 2.Equal volume normal saline were injected intravenously in Group 3.The expression of SP-B in the lung tissue was mesured by enzyme-linked immunosorbent assay(ELISA) and the concentration of phospholipid in bronchoalveolar lavage fluid(BALF) was detected by colorimetry in five sacrificed rodents of every group before administration as well as 30 min,1h and 2h after administration.Results:The level of SP-B and phospholipid had no significant difference among the three groups before administration.(P > 0.05).The concentrations of SP-B and phospholipid in Group 1 were significantly higher than Group 2(P < 0.05) and group 3(P < 0.01) at 30 min,1 h and 2h after ulinastatin treatment Further,the production of SP-B and phospholipid in Group 2 were also more than Group 3 at the same time.(P < 0.05).Conclusions:Ulinastatin has a curative effect on acute lung injury caused by severe heat-stroke.These effect may be related to improve the decrease of SP-B and phospholipid in lung tissue of severe heat-stroke rats effectively
引文
[1]全军重症医学专业委员会.2015热射病规范化诊断与治疗专家共识(草案).解放军医学杂志,2015,40(1):1-7.
[2]Varghese GM,John G,Thomas K,et al.Predictors of multi-organ dysfunction in heatstroke[J].Emerg Med J,2005;22(3):185-187.Tong H,Chen R,Yin H,et al.Mesenteric Lymph Duct Ligation Alleviating Lung Injury in Heat-stroke[J].Shock,2016,46(6):696-703.
[3]Pires-Neto RC,Morales MM,Lancas T,et al.Expression of acutephase cytokines,surfactant proteins,and epithelial apoptosis in small airways of human acute respiratory distress syndrome.J Crit Care.2013;28(1):111.e9-111.el5.Dushianthan A,Goss V,Cusack R,et al.Altered molecular specificity of surfactant phosphatidycholine synthesis in patients with acute respiratory distress syndrome.Respir Res.2014;15:128.
[4]Zhang LP,Zhao Y,Liu GJ,et al.Glabridin attenuates lipopolysaccharide-induced acute lung injury by inhibiting p38MAPK/ERK signaling pathway.Oncotarget.2016 Dec 27.doi:10.18632/oncotarget 14277.
[5]陈怿,童华生,张兴钦,等.乌司他丁减轻重症中暑大鼠肺的炎症和氧化损伤[J].中华急诊医学杂志,2014,24(8):884-890.
[6]Feng Z,Shi Q,Fan Y,et al.Ulinastatin and/or thymosin al for severe sepsis:A systematic review and meta-analysis J Trauma Acute Care Surg.2016;80(2):335-340;Karnad DR,Bhadade R,Verma PK,et al.Intravenous administration of ulinastatin(human urinary trypsin inhibitor)in severe sepsis;a multicenter randomized controlled study Intensive Care Med.2014;40(6):830-838.
[7]Mokra D,Kosutova P.Biomarkers in acute lung injury.Respir Physiol Neurobiol.2015;209:52-58.
[8]Wang LZ,Luo MY,Zhang JS,et al.Effect of ulinastatin on serum inflammatory factors in Asian patients with acute pancreatitis before and after treatment;a meta-analysis?.Int J Clin Pharmacol Ther.2016;54(11):890-898;Chen X,Wang Y,
[9]Luo H,et al.Ulinastatin reduces urinary sepsisrelated inflammation by upregulating IL10 and downregulating TNFa levels.Mol Med Rep.2013;8(1):29-34.
[10]Han S,Mallampalli RK.The Role of Surfactant in Lung Disease and Host Defense against Pulmonary InfectionsAnn Am Thorac Soc.2015;12(5):765-774.
[11]Lu B,Li MQ,Cheng SL.Clinical effectiveness of continuous blood purification in combination with ulinastatin in treating thermoplegia.Eur Rev Med Pharmacol Sci.2014;18(22):3464-3467.
[12]常月进,吴升,郝建等.不同剂量乌司他丁对高温气体致豚鼠急性肺损伤的影响[J].临床肺科杂志,2012,17(5):781-783.
[2]Varghese GM,John G,Thomas K,et al.Predictors of multi-organ dysfunction in heatstroke[J].Emerg Med J,2005;22(3):185-187.Tong H,Chen R,Yin H,et al.Mesenteric Lymph Duct Ligation Alleviating Lung Injury in Heat-stroke[J].Shock,2016,46(6):696-703.
[3]Pires-Neto RC,Morales MM,Lancas T,et al.Expression of acutephase cytokines,surfactant proteins,and epithelial apoptosis in small airways of human acute respiratory distress syndrome.J Crit Care.2013;28(1):111.e9-111.el5.Dushianthan A,Goss V,Cusack R,et al.Altered molecular specificity of surfactant phosphatidycholine synthesis in patients with acute respiratory distress syndrome.Respir Res.2014;15:128.
[4]Zhang LP,Zhao Y,Liu GJ,et al.Glabridin attenuates lipopolysaccharide-induced acute lung injury by inhibiting p38MAPK/ERK signaling pathway.Oncotarget.2016 Dec 27.doi:10.18632/oncotarget 14277.
[5]陈怿,童华生,张兴钦,等.乌司他丁减轻重症中暑大鼠肺的炎症和氧化损伤[J].中华急诊医学杂志,2014,24(8):884-890.
[6]Feng Z,Shi Q,Fan Y,et al.Ulinastatin and/or thymosin al for severe sepsis:A systematic review and meta-analysis J Trauma Acute Care Surg.2016;80(2):335-340;Karnad DR,Bhadade R,Verma PK,et al.Intravenous administration of ulinastatin(human urinary trypsin inhibitor)in severe sepsis;a multicenter randomized controlled study Intensive Care Med.2014;40(6):830-838.
[7]Mokra D,Kosutova P.Biomarkers in acute lung injury.Respir Physiol Neurobiol.2015;209:52-58.
[8]Wang LZ,Luo MY,Zhang JS,et al.Effect of ulinastatin on serum inflammatory factors in Asian patients with acute pancreatitis before and after treatment;a meta-analysis?.Int J Clin Pharmacol Ther.2016;54(11):890-898;Chen X,Wang Y,
[9]Luo H,et al.Ulinastatin reduces urinary sepsisrelated inflammation by upregulating IL10 and downregulating TNFa levels.Mol Med Rep.2013;8(1):29-34.
[10]Han S,Mallampalli RK.The Role of Surfactant in Lung Disease and Host Defense against Pulmonary InfectionsAnn Am Thorac Soc.2015;12(5):765-774.
[11]Lu B,Li MQ,Cheng SL.Clinical effectiveness of continuous blood purification in combination with ulinastatin in treating thermoplegia.Eur Rev Med Pharmacol Sci.2014;18(22):3464-3467.
[12]常月进,吴升,郝建等.不同剂量乌司他丁对高温气体致豚鼠急性肺损伤的影响[J].临床肺科杂志,2012,17(5):781-783.