基于量子点-血红蛋白体系用于Zn~(2+)的分析检测
详细信息 查看官网全文
- 英文论文题名：The analysis and detection of Zn~(2+) based on QDs-hemoglobin system
- 论文作者：常丽芳 ; 吴昊晟 ; 何锡文 ; 陈朗星 ; 张玉奎
- 英文论文作者：Lifang Chang ; Haocheng Wu ; Xiwen He ; Langxing Chen ; Yukui Zhang ; College of Chemistry ; University of Nankai ; Dalian Institute of Chemical Physics ; Chinese Academy of Sciences
- 年：2016
- 作者机构：南开大学化学学院;中国科学院大连化学物理研究所;
- 论文关键词：锰掺杂硫化锌量子点 ; 牛血红蛋白 ; Zn2+
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十三分会：复杂样品分离分析
- 语种：中文
- 分类号：O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十三分会 ; 复杂样品分离分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：143k
- 原文格式：D
- 会议级别：全国
摘要
在室温条件下,合成了水溶性锰掺杂硫化锌量子点~([1]),其水溶液具有很强的荧光,且发射光谱位于610 nm。随着牛血红蛋白的加入,与量子点发生共振能量转移作用,使得溶液的荧光强度降低约96%。而后,又向其中依次加入浓度增加的Zn~(2+),通过与血红蛋白发生特异性结合,导致荧光又逐渐恢复,线性范围为0.01 mM-2mM,检测限为22 nM。该量子点-血红蛋白体系在生理条件下,对Zn~(2+)具有很高的选择性及灵敏度,很强的抗干扰能力,且具有良好的水溶性、安全无毒,可用于复杂样品中Zn~(2+)的分析检测~([2])。此外,通过控制牛血红蛋白和Zn~(2+)的加入,可实现荧光的关-开模式,使得检测过程可视化。
The water-soluble Mn-doped ZnS QDs was synthesized at room temperature,and its solution has a strong emission in fluorescence spectrum at 610 nm.The fluorescent intensity can be decreased by 96%via resonance energy transfer interaction when hemoglobin with the increasing concentration was added,and then the fluorescence recovered upon the addition of gradient concentration of Zn~(2+) solution,with a linear range of 0.01 mM-2 mM,and detection limit of 22 nM.The QDs-hemoglobin system has a high selectivity and sensitivity and strong anti-interference ability for the detection of Zn~(2+) at physiological conditions,and thus can be applied to trace Zn~(2+)in complex samples with good water solubility and no toxicity.Besides,it can realize fluorescence off-on model,and visualize the process of detection controlled by addition of hemoglobin and Zn~(2+).
The water-soluble Mn-doped ZnS QDs was synthesized at room temperature,and its solution has a strong emission in fluorescence spectrum at 610 nm.The fluorescent intensity can be decreased by 96%via resonance energy transfer interaction when hemoglobin with the increasing concentration was added,and then the fluorescence recovered upon the addition of gradient concentration of Zn~(2+) solution,with a linear range of 0.01 mM-2 mM,and detection limit of 22 nM.The QDs-hemoglobin system has a high selectivity and sensitivity and strong anti-interference ability for the detection of Zn~(2+) at physiological conditions,and thus can be applied to trace Zn~(2+)in complex samples with good water solubility and no toxicity.Besides,it can realize fluorescence off-on model,and visualize the process of detection controlled by addition of hemoglobin and Zn~(2+).
引文
[1]Tu,R.;Liu,B.;Wang,Z.;Wang,H.Ana.l chem.2008,80:3458.
[2]黄池宝,梁兴,曾启华,陈华仕.高等学校化学学报,2015,36(4):646.
[2]黄池宝,梁兴,曾启华,陈华仕.高等学校化学学报,2015,36(4):646.