Thermodesulfatator autotrophicus sp.nov.,a thermophilic sulfate-reducing bacterium from the Indian Ocean

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• 英文论文题名：Thermodesulfatator autotrophicus sp.nov.,a thermophilic sulfate-reducing bacterium from the Indian Ocean
• 论文作者：Qiliang Lai ; Junwei Cao ; Samuel Dupont ; Zongze Shao ; Mohamed Jebbar ; Karine Alain
• 英文论文作者：Qiliang Lai ; Junwei Cao ; Samuel Dupont ; Zongze Shao ; Mohamed Jebbar ; Karine Alain ; Key Laboratory of Marine Genetic Resources ; The Third Institute of State Oceanic Administration ; Université de Bretagne Occidentale (UBO ; UEB) ; Institut Universitaire Européen de la Mer (IUEM)-UMR 6197 ; Laboratoire de Microbiologie des Environnements Extremes (LM2E) ; Rue Dumont d'Urville ; CNRS ; IUEM-UMR 6197 ; Laboratoire de Microbiologie des Environnements Extremes (LM2E) ; Rue Dumont d'Urville ; Ifremer ; UMR 6197 ; Laboratoire de Microbiologie des Environnements Extremes (LM2E) ; Technopole Pointe du diable ; School of Municipal and Environmental Engineering ; Harbin Institute of Technology
• 年：2016
• 作者机构：Key Laboratory of Marine Genetic Resources,The Third Institute of State Oceanic Administration;Université de Bretagne Occidentale (UBO,UEB),Institut Universitaire Européen de la Mer (IUEM)-UMR 6197,Laboratoire de Microbiologie des Environnements Extremes (LM2E),Rue Dumont d'Urville;CNRS,IUEM-UMR 6197,Laboratoire de Microbiologie des Environnements Extremes (LM2E),Rue Dumont d'Urville;Ifremer,UMR 6197,Laboratoire de Microbiologie des Environnements Extremes (LM2E),Technopole Pointe du diable;School of Municipal and Environmental Engineering,Harbin Institute of Technology;
• 会议召开时间：2016-11-11
• 会议录名称：福建省海洋学会2016年学术年会论文摘要集
• 语种：英文
• 分类号：Q93
• 学会代码：FJHY
• 会议名称：福建省海洋学会2016年学术年会
• 会议地点：中国福建厦门
• 主办单位：福建省海洋学会、福建省水产研究所
• 学会名称：福建省海洋学会
• 页数：1
• 文件大小：48k
• 原文格式：O
• 会议级别：地方

摘要

A novel sulfate-reducing bacterium,strain S606~T,was isolated from a sulfide sample collected at a depth of 2764 m from a deep-sea vent chimney wall in the Indian Ocean.Phylogenetic 16 S rRNA gene sequence analyses placed strain S606~T within the genus Thermodesulfatator,with highest sequence similarity of 98.2%to Thermodesulfatator indicus DSM 15286~T,followed by Thermodesulfatator atlanticus AT1325~T(97.4%).The average nucleotide identity(ANI) values between strain S606~T and the two other type strains(T.indicus DSM 15286~T and T.atlanticus AT1325~T) were 79.2%and 71.5%,respectively.The digital DNA-DNA hybridization estimate values between strain S606~T and these two type strains were22.7±2.4%and 18.1=1=2.3%,respectively.Cells were Gram-negative,anaerobic,motile rods(1-1.8×0.5-0.7 μm).The novel isolate grew at NaCl concentrations ranging from 1.5 to 4.5%(optimum2.5-3%),from pH 5.5 to 8(optimum 6.5-7) and at temperatures between 50 and 80 ℃(optimum65-70 ℃).Strain S606 grew chemolithoautotrophically in an H_2/CO_2 atmosphere(80/20,v/v;200 kPa),used sulfate as a terminal electron acceptor,but not sulfur,sulfite nor thiosulfate.The predominant fatty acids were C_(16:0)(24.2%),summed feature 8(C_(18:1) ω6c and/or C_(18:1) ω7c,26.3%).C_(18:0)(22.2%) and C_(18:1) ω9c(9.2%).The DNA G+C content of the chromosomal DNA was 43.1mol%.The combined genotypic,chemotaxonomic and phenotypic traits show that strain S606~T should be described as a novel species of the genus Thermodesulfatator,for which the name Thermodesulfatator autotrophicus sp.nov.is proposed.The type strain is S606~T(= DSM 101864~T= MCCC 1A01871~T)
A novel sulfate-reducing bacterium,strain S606~T,was isolated from a sulfide sample collected at a depth of 2764 m from a deep-sea vent chimney wall in the Indian Ocean.Phylogenetic 16 S rRNA gene sequence analyses placed strain S606~T within the genus Thermodesulfatator,with highest sequence similarity of 98.2%to Thermodesulfatator indicus DSM 15286~T,followed by Thermodesulfatator atlanticus AT1325~T(97.4%).The average nucleotide identity(ANI) values between strain S606~T and the two other type strains(T.indicus DSM 15286~T and T.atlanticus AT1325~T) were 79.2%and 71.5%,respectively.The digital DNA-DNA hybridization estimate values between strain S606~T and these two type strains were22.7±2.4%and 18.1=1=2.3%,respectively.Cells were Gram-negative,anaerobic,motile rods(1-1.8×0.5-0.7 μm).The novel isolate grew at NaCl concentrations ranging from 1.5 to 4.5%(optimum2.5-3%),from pH 5.5 to 8(optimum 6.5-7) and at temperatures between 50 and 80 ℃(optimum65-70 ℃).Strain S606 grew chemolithoautotrophically in an H_2/CO_2 atmosphere(80/20,v/v;200 kPa),used sulfate as a terminal electron acceptor,but not sulfur,sulfite nor thiosulfate.The predominant fatty acids were C_(16:0)(24.2%),summed feature 8(C_(18:1) ω6c and/or C_(18:1) ω7c,26.3%).C_(18:0)(22.2%) and C_(18:1) ω9c(9.2%).The DNA G+C content of the chromosomal DNA was 43.1mol%.The combined genotypic,chemotaxonomic and phenotypic traits show that strain S606~T should be described as a novel species of the genus Thermodesulfatator,for which the name Thermodesulfatator autotrophicus sp.nov.is proposed.The type strain is S606~T(= DSM 101864~T= MCCC 1A01871~T)

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