一种水凝胶敷料的制备及其对SD大鼠深Ⅱ度烧伤创面的愈合作用
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- 英文论文题名：Preparation of Hydrogel Dressings and Its Effect on The Healing of Deep Second Degree Burn Wounds in SD Rats
- 论文作者：张丽娟 ; 韩潇扬 ; 高原 ; 王卓 ; 姚翀 ; 高申
- 英文论文作者：ZHANG Lijuan ; HAN Xiaoyang ; GAO Yuan ; WANG Zhuo ; YAO Chong ; SHEN Gao ; Department of Pharmaceutics ; Changhai Hospital ; Second Military Medical University ; Outpatient Department of Space City ; The 306th Hospital of PLA
- 年：2016
- 作者机构：第二军医大学长海医院药学部;解放军总装备部后勤部航天城门诊部药局;
- 论文关键词：水凝胶敷料 ; SD大鼠 ; 深Ⅱ度烧伤 ; 创面愈合
- 英文论文关键词：hydrogel dressing ; SD rats ; deep second degree burn ; wound healing
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R943;R96
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：8
- 文件大小：2593k
- 原文格式：O
- 会议级别：全国
摘要
目的制备一种水凝胶敷料并考察其对SD大鼠深Ⅱ度烧伤创面的愈合作用。方法:制备水凝胶敷料,并筛选其灭菌方法。采用新西兰兔考察其皮肤刺激性。采用热固体烫伤法制备SD大鼠深Ⅱ度烧伤模型,采用清得佳及长海医院院内制剂脱痂软膏为阳性对照,考察水凝胶敷料对其创面愈合作用。结果:制备的水凝胶敷料澄清、透明,黏度与阳性对照药物清得佳一致。辐射灭菌后,水凝胶敷料黏度急剧下降,高压蒸汽灭菌前后,黏度无显著性变化。水凝胶敷料对兔皮肤无显著刺激性。与清得佳及脱痂软膏相比,实验组愈合时间无显著性差异;与模型对照组相比,愈合时间有显著性差异(P<0.01)。结论:以羟乙基纤维素为基质制备的水凝胶敷料,黏度适宜,澄清透明,易于观察创面愈合情况,无显著皮肤刺激性,可显著促进SD大鼠深Ⅱ度烧伤创面的愈合,具有广阔的应用前景。
OBJECTTVE Prepare a hydrogel dressing and investigate its effect on the healing of deep second degree burn wounds in SD rats.METHODS Prepare a hydrogel dressing and screen its sterilization method.The skin irritation of hydrogel dressing was investigated by New Zealand rabbits.A deep second degree burn model of SD rats was prepared by hot solid scald method.Tuojiaointment and IntrasiteGel were adopted as positive controls to investigate its effect on the wound healing.RESULTS The hydrogel dressing was clear and transparent,and the viscosity was well consistent with Intrasite Gel.After radiation sterilization,the viscosity of the hydrogel was sharply decreased.However,the viscosity was not significantly changed before and after high pressure steam sterilization.The hydrogel had no significant skin irritation.The healing time of the experimental group was not significantly better than that of the positive control,but has significant difference compared with the vehicle control group(P<0.01).CONCLUSIONSHydroxyethyl cellulose was used as the matrix of hydrogel dressing.The hydrogel dressing prepared has appropriate viscosity,clarification,and no obvious skin irritation.Besides,it hasremarkable healing effectof deep second degree burn wounds in SD rats.Above all,it has broad application prospects.
OBJECTTVE Prepare a hydrogel dressing and investigate its effect on the healing of deep second degree burn wounds in SD rats.METHODS Prepare a hydrogel dressing and screen its sterilization method.The skin irritation of hydrogel dressing was investigated by New Zealand rabbits.A deep second degree burn model of SD rats was prepared by hot solid scald method.Tuojiaointment and IntrasiteGel were adopted as positive controls to investigate its effect on the wound healing.RESULTS The hydrogel dressing was clear and transparent,and the viscosity was well consistent with Intrasite Gel.After radiation sterilization,the viscosity of the hydrogel was sharply decreased.However,the viscosity was not significantly changed before and after high pressure steam sterilization.The hydrogel had no significant skin irritation.The healing time of the experimental group was not significantly better than that of the positive control,but has significant difference compared with the vehicle control group(P<0.01).CONCLUSIONSHydroxyethyl cellulose was used as the matrix of hydrogel dressing.The hydrogel dressing prepared has appropriate viscosity,clarification,and no obvious skin irritation.Besides,it hasremarkable healing effectof deep second degree burn wounds in SD rats.Above all,it has broad application prospects.
引文
[1]TAVARES PEREIRA DDOS S,LIMA RIBEIRO MH,DE PONTES FILHO NT,et al.Development of animal model for studying deep second-degree thermal burns[J].Journal of Biomedicine&Biotechnology,2012,201 2:1-7.
[2]STASHAK T S,FARSTVEDTV E.OTHIC A.Update on wound dressings:indications and best use[J].Clinical Techniques in Equine Practice,2004,3(2):148-163.
[3]LUO J B,FANG G F,XIE X Y.Progress in the studies on hydrogel burn dressings[J].J Biomed Eng(生物医学工程杂志),2004,21(1):156-159.
[4]JONES A,VAUGHAN D.Hydrogel dressings in the management of a variety of wound types:A review[J].Journal of Orthopaedic Nursing,2005,9(Suppl,1):S 1-S11.
[5]MAHER M S,ANTAR A.MEBT/MEBO Changing the ordinary concept of burn wound management[J].The Chinese Journal of Burns Wounds&Surface Ulcers(中国烧伤疮疡杂志),2013,25(5):332-341.
[6]STRAND O,SAN MIGUEL L,ROWAN S,et al.Retrospective comparison of two years in a paediatric bums unit,with and without acticoat as a standard dressing[J].Annals of Burns and Fire Disasters,2010,X X III(4):182-185.
[7]PILAKASIRI K MP,SRINGERNYUANG D.SANGJUN N,el al.Efficacy of chitin-PAA-GTMAC gel in promoting wound healing:animal study[J].Journal of Materials Science Materials in Medicine,2011,22(11):2497-2504.
[8]HAJIAGHAALIPOUR F,KANTHIMATHI MS,ABDULLA MA,et al.The Effect of Camellia sinensis on Wound Healing Potential in an Animal Model[J].Evidence-based Complementary and Alternative Medicine:eCAM,2013,2013:1-7.
[9]GETHTN G,COWMAN S.Manuka honey vs.hydrogel—a prospective,open label,multicentre,randomised controlled trial to compare desloughing efficacy and healing outcomes in venous ulcers[J].Journal ofClinical Nursing,2009,l8(3):466-474.
[10]ZHANG J P,WEI YH,ZHANG G Q,et al.Dermal Irritation and Long-term Toxicity of Total Alkaloid of Sophora Alopecuroides Thermo-sensitive Gel[J].Chinese Journal of Modern Applied Pharmacy(中国现代应用程序),2015,32(10):1180-1184.
[11]HU J H.Dermatoptermacology(皮肤药理学)[M].Beijing:Chemical Industry Press,2008:259-261.
[12]LIU Q,DIAO B,ZHANG Y Progress in preparation methodology of animal models of bum injuries[J].Laboratory Animal and Comparative Medicine(实验动物与比较医学),2013,33(2):162-168.
[13]YU W R.SUN JL,CHEN Y h,et al.The effect of traditional Chinese medicine tuojiagao on bum wound[J].Academic Journal of Second Militray Medical University(第二军医大学学报),1999,20(3):167-168.
[14]YU W R,BEN DF.Effect of the Chinese traditional medicine'Tuojiagao"on hydrofluoric acid bums in shipmen[J].Chin J Naut Med&Hyperbar Med(中华航海医学与高气压医学杂志),2006,13(3):150-152.
[15]GONG Z H,YAO J,JI J F,ef al.Effect of ionic silver dressing combined with hydrogel on degree II bum wound healing[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2009,13(42):8373-8376.
[16]AZIZ Z,ABU SF,CHONG N J.A systematic review of silver-containing dressings and topical silver agents(used with dressings)for bum wounds[J].Burns:Journal of the International Society for Burn Injuries,2012,38(3):'S07-318.
[17]MAHMMOD AA.KHALED AA,HAPIPAH MA,et al.Wound healing activities of Rafflesia Hasseltii extract in rats[J].J Clin Biochem Nutr,2009,45(3):304-308.
[18]YANG H Z,CHEN S,CHEN Z H,et al.Multi-center clinical trial of FLAMIGEL(hydrogel dressing)for the treatment of residual burn wound[J].Chin J Burns(中华烧伤杂志),2013,29(2):177-180.
[19]DAVE L V P,ANTOON L,DANNY],et al.Treatment of recalcitrant wounds of diverse etiology with a new hydroactive gel[J].Wounds,2009,21(9):243-248.
[20]CHAO S W,LI Y,WANG X Z,et al.Application of hydroactive colloid wound gel in bum wound[J].Chin J Injury Repair Wound Healing(中华损伤与修复杂志),2008,3(3):9-10.
[21]ZHONG Z F,ZHOU Q,LI H X,et al.Clinical application of instrasite on the surfers burns[J].Chin J Prim Med Pharm(中国基层医药),2005,12(1):77-78.
[22]GE H,ZHANG DL,YANG G H.Biological effect of hydrogel on residual bum wounds[J].Chinese Journal of Histochemistry and Cytochemistry(中国组织化学与细胞化学杂志),2010,19(1):58-65.
[2]STASHAK T S,FARSTVEDTV E.OTHIC A.Update on wound dressings:indications and best use[J].Clinical Techniques in Equine Practice,2004,3(2):148-163.
[3]LUO J B,FANG G F,XIE X Y.Progress in the studies on hydrogel burn dressings[J].J Biomed Eng(生物医学工程杂志),2004,21(1):156-159.
[4]JONES A,VAUGHAN D.Hydrogel dressings in the management of a variety of wound types:A review[J].Journal of Orthopaedic Nursing,2005,9(Suppl,1):S 1-S11.
[5]MAHER M S,ANTAR A.MEBT/MEBO Changing the ordinary concept of burn wound management[J].The Chinese Journal of Burns Wounds&Surface Ulcers(中国烧伤疮疡杂志),2013,25(5):332-341.
[6]STRAND O,SAN MIGUEL L,ROWAN S,et al.Retrospective comparison of two years in a paediatric bums unit,with and without acticoat as a standard dressing[J].Annals of Burns and Fire Disasters,2010,X X III(4):182-185.
[7]PILAKASIRI K MP,SRINGERNYUANG D.SANGJUN N,el al.Efficacy of chitin-PAA-GTMAC gel in promoting wound healing:animal study[J].Journal of Materials Science Materials in Medicine,2011,22(11):2497-2504.
[8]HAJIAGHAALIPOUR F,KANTHIMATHI MS,ABDULLA MA,et al.The Effect of Camellia sinensis on Wound Healing Potential in an Animal Model[J].Evidence-based Complementary and Alternative Medicine:eCAM,2013,2013:1-7.
[9]GETHTN G,COWMAN S.Manuka honey vs.hydrogel—a prospective,open label,multicentre,randomised controlled trial to compare desloughing efficacy and healing outcomes in venous ulcers[J].Journal ofClinical Nursing,2009,l8(3):466-474.
[10]ZHANG J P,WEI YH,ZHANG G Q,et al.Dermal Irritation and Long-term Toxicity of Total Alkaloid of Sophora Alopecuroides Thermo-sensitive Gel[J].Chinese Journal of Modern Applied Pharmacy(中国现代应用程序),2015,32(10):1180-1184.
[11]HU J H.Dermatoptermacology(皮肤药理学)[M].Beijing:Chemical Industry Press,2008:259-261.
[12]LIU Q,DIAO B,ZHANG Y Progress in preparation methodology of animal models of bum injuries[J].Laboratory Animal and Comparative Medicine(实验动物与比较医学),2013,33(2):162-168.
[13]YU W R.SUN JL,CHEN Y h,et al.The effect of traditional Chinese medicine tuojiagao on bum wound[J].Academic Journal of Second Militray Medical University(第二军医大学学报),1999,20(3):167-168.
[14]YU W R,BEN DF.Effect of the Chinese traditional medicine'Tuojiagao"on hydrofluoric acid bums in shipmen[J].Chin J Naut Med&Hyperbar Med(中华航海医学与高气压医学杂志),2006,13(3):150-152.
[15]GONG Z H,YAO J,JI J F,ef al.Effect of ionic silver dressing combined with hydrogel on degree II bum wound healing[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2009,13(42):8373-8376.
[16]AZIZ Z,ABU SF,CHONG N J.A systematic review of silver-containing dressings and topical silver agents(used with dressings)for bum wounds[J].Burns:Journal of the International Society for Burn Injuries,2012,38(3):'S07-318.
[17]MAHMMOD AA.KHALED AA,HAPIPAH MA,et al.Wound healing activities of Rafflesia Hasseltii extract in rats[J].J Clin Biochem Nutr,2009,45(3):304-308.
[18]YANG H Z,CHEN S,CHEN Z H,et al.Multi-center clinical trial of FLAMIGEL(hydrogel dressing)for the treatment of residual burn wound[J].Chin J Burns(中华烧伤杂志),2013,29(2):177-180.
[19]DAVE L V P,ANTOON L,DANNY],et al.Treatment of recalcitrant wounds of diverse etiology with a new hydroactive gel[J].Wounds,2009,21(9):243-248.
[20]CHAO S W,LI Y,WANG X Z,et al.Application of hydroactive colloid wound gel in bum wound[J].Chin J Injury Repair Wound Healing(中华损伤与修复杂志),2008,3(3):9-10.
[21]ZHONG Z F,ZHOU Q,LI H X,et al.Clinical application of instrasite on the surfers burns[J].Chin J Prim Med Pharm(中国基层医药),2005,12(1):77-78.
[22]GE H,ZHANG DL,YANG G H.Biological effect of hydrogel on residual bum wounds[J].Chinese Journal of Histochemistry and Cytochemistry(中国组织化学与细胞化学杂志),2010,19(1):58-65.