液相色谱-串联高分辨质谱测定鸡肉中卡巴氧、喹乙醇的代谢物残留
详细信息 查看官网全文
- 英文论文题名：Determination of metabolites of carbadox and olaquindox in chicken by LC-HRMS
- 论文作者：陆桂萍 ; 曲斌 ; 耿士伟 ; 蒋天梅
- 英文论文作者：LU Guiping ; QU Bin ; GENG Shiwei ; JIANG Tianmei ; Jiangsu Quality Inspection and Testing Center for Animal Products
- 年：2016
- 作者机构：江苏省畜产品质量检验测试中心;
- 论文关键词：卡巴氧 ; 喹乙醇 ; 代谢物 ; 喹喔啉-2-羧酸 ; 3-甲基-喹喔啉-2-羧酸 ; 液相色谱-串联高分辨质谱 ; 残留
- 英文论文关键词：carbadox ; olaquindox ; metabolite ; quinoxaline-2-carboxylic acid(QCA) ; methyl-3-quinoxaline-2-carboxylic acid(mQCA) ; residual ; LC-HRMS
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S859.84;O657.63
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：5
- 文件大小：719k
- 原文格式：O
- 会议级别：全国
摘要
建立了鸡肉中卡巴氧、喹乙醇的代谢物残留的液相色谱-串联高分辨质谱测定方法。鸡肉样品经酸解后,固相萃取净化,液相色谱-串联四极杆静电场轨道阱高分辨质谱测定,稳定同位素内标定量。卡巴氧、喹乙醇的代谢物喹喔啉-2-羧酸、3-甲基-喹喔啉-2-羧酸在0.5μg/kg~10μg/kg范围内线性关系良好,最低定量限0.5μg/kg,高中低浓度精密度<20%(RSD%),平均回收率70%~120%。经实际样品的测定,本方法简便、实用,适宜于大批量样品的测定。
An LC-HRMS method was established to determine the metabolites of carbadox and olaquindox in chicken.The samples were acidified,and cleaned-up by SPE.The analytes,quinoxaline-2-carboxylic acid(QCA) and methyl-3-quinoxaline-2-carboxylic acid(mQCA),metabolites of carbadox and olaquindox,were determined by LC-quadrupole Orbitrap MS.Stable isotope was used as internal standard.QCA and mQCA were showed good linearity at the range between 0.5μg/kg and 10 μg/kg.The limit of quantitation was 0.5 μg/kg.The intra-and inter-precision were lower 20%and the average recovery was between 70%and120%.After practice of real samples,the method was simple and suitable to high throughput large scale samples.
An LC-HRMS method was established to determine the metabolites of carbadox and olaquindox in chicken.The samples were acidified,and cleaned-up by SPE.The analytes,quinoxaline-2-carboxylic acid(QCA) and methyl-3-quinoxaline-2-carboxylic acid(mQCA),metabolites of carbadox and olaquindox,were determined by LC-quadrupole Orbitrap MS.Stable isotope was used as internal standard.QCA and mQCA were showed good linearity at the range between 0.5μg/kg and 10 μg/kg.The limit of quantitation was 0.5 μg/kg.The intra-and inter-precision were lower 20%and the average recovery was between 70%and120%.After practice of real samples,the method was simple and suitable to high throughput large scale samples.
引文
[1]曹随忠,张力,梁建平,等.喹恶啉-1,4-二氧化物类抗菌促生长剂特殊毒理学研究进展[J].动物医学进展,2001,22(2):17-20
[2]宋春美,侯玉泽,刘宣兵,等.喹乙醇的危害及检测方法研究进展[J].河南农业科学,2009(12):13-17
[3]中华人民共和国农业部公告第168号.饲料药物添加剂使用规范[M].北京:中国农业科学技术出版社,2014:122
[4]范立鹏,段振娟,方国臻,等.动物源性食品中喹恶啉类药物残留检测方法研究进展[J].中国食品学报,2012,12(9):171-178
[5]吴玉杰,陈冬梅,陶燕飞,等.高效液相色谱法测定动物组织中3-甲基喹喔啉-2-羧酸残留量[J].分析科学学报,2009,25(4):427-430
[6]薛良辰,彭玉芬,刘陆,等.超高效液相色谱-串联质谱法快速检测水产品中喹乙醇及其代谢物3-甲基喹恶啉-2-羧酸的残留[J].现代食品科技,2013,29(2):413-415
[7]Le T,Yu H,Niu XD.Detecting quinoxaline-2-carboxylic acid in animal tissues by using sensitive rapid enzyme-linked immunosorbent assay and time-resolved fiuoroimmunoassay[J].Food Chem,2015,175:85-91
[8]Merou A,Kaklamanos G Theodoridis G Determination of carbadox and metabolites of carbadox and olaquindox in muscle tissue using high performance liquid chromatography-tandem mass spectrometry[J].J Chromatogr B,2012,881:90-95
[9]郭霞,孙振中,戚隽渊,等.南美白对虾中喹乙醇及其代谢物3-甲基喹恶啉-2-羧酸残留的高效液相色谱-串联质谱检测[J].中国农业大学学报,2014,19(1):156-160
[2]宋春美,侯玉泽,刘宣兵,等.喹乙醇的危害及检测方法研究进展[J].河南农业科学,2009(12):13-17
[3]中华人民共和国农业部公告第168号.饲料药物添加剂使用规范[M].北京:中国农业科学技术出版社,2014:122
[4]范立鹏,段振娟,方国臻,等.动物源性食品中喹恶啉类药物残留检测方法研究进展[J].中国食品学报,2012,12(9):171-178
[5]吴玉杰,陈冬梅,陶燕飞,等.高效液相色谱法测定动物组织中3-甲基喹喔啉-2-羧酸残留量[J].分析科学学报,2009,25(4):427-430
[6]薛良辰,彭玉芬,刘陆,等.超高效液相色谱-串联质谱法快速检测水产品中喹乙醇及其代谢物3-甲基喹恶啉-2-羧酸的残留[J].现代食品科技,2013,29(2):413-415
[7]Le T,Yu H,Niu XD.Detecting quinoxaline-2-carboxylic acid in animal tissues by using sensitive rapid enzyme-linked immunosorbent assay and time-resolved fiuoroimmunoassay[J].Food Chem,2015,175:85-91
[8]Merou A,Kaklamanos G Theodoridis G Determination of carbadox and metabolites of carbadox and olaquindox in muscle tissue using high performance liquid chromatography-tandem mass spectrometry[J].J Chromatogr B,2012,881:90-95
[9]郭霞,孙振中,戚隽渊,等.南美白对虾中喹乙醇及其代谢物3-甲基喹恶啉-2-羧酸残留的高效液相色谱-串联质谱检测[J].中国农业大学学报,2014,19(1):156-160