培养条件对蒿柳花粉萌发及花粉管生长的影响
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- 英文论文题名：Effects of Culture Conditions on Pollen Germination and Pollen Tube Growth of Salix viminalis
- 论文作者：彭向永 ; 刘俊祥 ; 李振坚 ; 程运河 ; 孙振元
- 英文论文作者：PENG Xiang-yong ; LIU Jun-xiang ; LI Zhen-jian ; CHENG Yun-he ; SUN Zhen-yuan ; Research Institute of Forestry ; Chinese Academy of Forestry ; Key Laboratory of Tree Breeding and Cultivation ; State Forestry Administration
- 年：2016
- 作者机构：中国林业科学研究院林业研究所;国家林业局林木培育重点实验室;
- 论文关键词：蒿柳 ; 花粉萌发 ; 离体萌发 ; 萌发率 ; 花粉管
- 英文论文关键词：Salix viminalis ; Pollen germination ; in vitro germination ; Germination rate ; Pollen tube
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S793.9
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：5
- 文件大小：693k
- 原文格式：O
- 会议级别：全国
摘要
采用花粉离体培养法,研究不同的温度、光照及培养基组分对蒿柳花粉萌发的影响。结果表明:培养温度和光照强度均影响蒿柳花粉萌发率和花粉管长度,20~25℃是蒿柳花粉萌发的最适温度区间,1500lx和2000lx是蒿柳花粉萌发的最佳光照强度。正交试验中,一定浓度的蔗糖、H_3BO_3及pH值均可显著影响蒿柳花粉萌发,3个因子最佳组合为蔗糖100g·L~(-1)+H_3BO_3 200mg·L~(-1)+pH5.0,在光照强度为1000lx,温度为25℃时,萌发率高于48.8%。利用试验筛选出的蒿柳花粉最佳离体培养组合培养花粉,并与花粉在柱头上活体萌发相比较,二者花粉管长度无显著差异。
The effects of temperature,light intensity and culture medium composition on Salix viminalis pollen germination and pollen tube growth were investigated with in vitro culture.The results were showed that Salix viminalis pollen germination and tube growth could be affected by cultivation temperature and light intensity.Pollen germination of the optimum temperature range was 15-25 t,and the highest germination rate was under the light intensity of 1500 lx and 2000 lx.Sucrose,H_3BO_3and pH value could promote pollen germination within a certain concentration range remarkably in orthogonal design.The optimal culture medium was sucrose 100 g · L~(-1) + H_3BO_3 200 mg · L~(-1) + pH5.0,and in the medium the rate of pollen germination was higher than 48.8%under the conditions of 1000 lx and 25℃.Pollen germination was conducted on the best combination with in vitro was no significant differences compared with pollen germination on stigma.
The effects of temperature,light intensity and culture medium composition on Salix viminalis pollen germination and pollen tube growth were investigated with in vitro culture.The results were showed that Salix viminalis pollen germination and tube growth could be affected by cultivation temperature and light intensity.Pollen germination of the optimum temperature range was 15-25 t,and the highest germination rate was under the light intensity of 1500 lx and 2000 lx.Sucrose,H_3BO_3and pH value could promote pollen germination within a certain concentration range remarkably in orthogonal design.The optimal culture medium was sucrose 100 g · L~(-1) + H_3BO_3 200 mg · L~(-1) + pH5.0,and in the medium the rate of pollen germination was higher than 48.8%under the conditions of 1000 lx and 25℃.Pollen germination was conducted on the best combination with in vitro was no significant differences compared with pollen germination on stigma.
引文
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21.吴开志,肖千文,廖运洪,等.2008.核桃花粉离体萌发的培养基研究[J].果树学报,25(6):941-945.
22.武喆,刘霞,张光星.2010.不同温度对胡萝卜花粉活力的影响[J].华北农学报,25(4):116-118.
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25.杨中汉,廖祥儒.1994.照光对兰州百合花粉萌发和花粉管生长的影响[J].北京大学学报(自然科学版),30(2):239-244.
26.姚成义,赵洁.钙和硼对蓝猪耳花粉萌发及花粉管生长的影响[J].武汉植物学研究,2004,22(1):1-7.
27.张绍铃,陈迪新,康琅,等.2005.培养基组分及pH值对梨花粉萌发和花粉管生长的影响[J].西北植物学报,25(2):225-230.
28.赵丽娟,李淑娟,于金海,等.2011.小黑杨花粉离体萌发和细胞学分析[J].林业科学,47(6):36-41.
2.Dai XG,Hu QJ,Cai QL,et al.2014.The willow genome and divergent evolution from poplar after the common genome duplication[J].Cell Research,24:1274-1277.
3.Spielman ML,Preuss D,Li FL,et al.1997.Tetraspore is required for male meiotic cytokinesis in Arabidopsis thaliana[J].Development,124,2645-2657.
4.Stanley RG,Linskens HF.1974.Pollen:biology,biochemistry,management[M].Berlin:Springer-Verlag Illustrations,307-308.
5.Weaver ML,Timm H.1988.Influence of temperature and water status on pollen viability in bean[J].Journal of American Society of Horticultural Science,113(1):13-15.
6.Zhai FF,Mao JM,Liu JX,et al.2016.Male and female subpopulations of Salix viminalis present high genetic diversity and high long-term migration rates between them[J].Front.Plant Sci.7:330.doi:10.3389/fpls.2016.00330.
7.陈文涛,袁德义,张日清,等.2013.硼及植物生长调节剂对枣花粉萌发的影响[J].江西农业大学学报,35(3):496-501.
8.崔丽娟,李伟,张曼胤,等.2011.不同湿地植物对污水中氮磷去除的贡献[J].湖泊科学,23(2):203-208.
9.董易,马晓红,沈强,等.2012.荷兰水仙花粉萌发和花粉贮藏性[J].上海交通大学学报(农业科学版),30(5):24-29.
10.杜玉虎,张绍铃,姜雪婷,等.2006.梅花粉离体萌发及花粉管生长特性研究[J].西北植物学报,6(9):1846-1852.
11.顾钢,苏学强,林伟杰,等.1999.橄榄花粉生活力相关因素对育种的影响[J].福建果树,(3):1-4.
12.郭磊,张斌斌,马瑞娟,等.2014.温度对桃离体花药散粉及花粉萌发的影响[J].植物生理学报,50(3):269-274.
13.郭思佳,朱玉菲,刘冬云,等.2014.有斑百合花粉培养条件的研究[J].河北农业大学学报,37(3):24-28.
14.韩志强,袁德义,陈文涛,等.2014.不同营养元素及其配比对枣花粉萌发与花粉管生长的影响[J].江西农业大学学报,36(2):357~363.
15.李旭新,张艳青,冯献宾,等.2009.不同培养条件对黄连木花粉萌发和花粉管生长的影响[J].西北植物学报,29(5):867-873.
16.吕晋慧,赵耀,任意,等.2011.地被菊花粉生活力测定及影响因素研究[J].华北农学报,2011,26(4):189-193.
17.马力耕,崔素娟,徐小冬,等.1997.G蛋白在细胞外钙调素启动花粉萌发和花粉管伸长中的作用[J].自然科学进展,7(6):751-754.
18.马力耕,崔素娟,徐小冬,等.1998.肌醇磷脂信号途径参与胞外钙调素启动花粉萌发和花粉管伸长[J].植物生理学报,24(2):196-200.
19.尚宏芹.2011.培养基成分对芍药花粉萌发及花粉管生长的影响[J].山地农业生物学报,30(4):323-327.
20.王改萍,杨洪宁,倪果果,等.2009.楸树等中梓属树种花粉离体培养条件下的研究[J].植物资源与环境学报,18(2):34-42.
21.吴开志,肖千文,廖运洪,等.2008.核桃花粉离体萌发的培养基研究[J].果树学报,25(6):941-945.
22.武喆,刘霞,张光星.2010.不同温度对胡萝卜花粉活力的影响[J].华北农学报,25(4):116-118.
23.徐进,陈天华,王章荣,等.1998.不同贮藏方法及光照对马尾松花粉活力的影响[J].南京林业大学学报,22(3):71-74.
24.许珂,古松,江莎.2008.金银忍冬花粉离体萌发初探[J].热带亚热带植物学报,16(2):109-115.
25.杨中汉,廖祥儒.1994.照光对兰州百合花粉萌发和花粉管生长的影响[J].北京大学学报(自然科学版),30(2):239-244.
26.姚成义,赵洁.钙和硼对蓝猪耳花粉萌发及花粉管生长的影响[J].武汉植物学研究,2004,22(1):1-7.
27.张绍铃,陈迪新,康琅,等.2005.培养基组分及pH值对梨花粉萌发和花粉管生长的影响[J].西北植物学报,25(2):225-230.
28.赵丽娟,李淑娟,于金海,等.2011.小黑杨花粉离体萌发和细胞学分析[J].林业科学,47(6):36-41.
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