人参复合多糖提高猪圆环病毒疫苗免疫效果的研究
详细信息 查看官网全文
- 英文论文题名：Of Ginseng polysaccharides complex enhanced immune response to Porcine Circovirus in pigs
- 论文作者：孙加节 ; 蒋勇 ; 习欠云 ; 张永亮
- 英文论文作者：Sun Jiajie ; Jiang Yong ; Xi Qianyun ; Zhang Yongliang ; Colloege of Animal Science ; South China Agricultural University ; National Engineering Research Center for Breeding Swine Industry
- 年：2016
- 作者机构：华南农业大学动物科学学院国家生猪种业工程技术研究中心;
- 论文关键词：仔猪 ; 人参复合多糖 ; 猪圆环病毒疫苗 ; 免疫
- 英文论文关键词：piglets ; Ginseng polysaccharides complex ; Porcine circovirus ; immunity
- 会议召开时间：2016-06-14
- 会议录名称：第25届广东省科技进步活动月畜牧兽医学术与科技创新发展大会论文集
- 语种：中文
- 分类号：S853.74
- 学会代码：GDKL
- 会议名称：第25届广东省科技进步活动月畜牧兽医学术与科技创新发展大会
- 会议地点：中国广东广州
- 主办单位：广东省畜牧兽医学会
- 学会名称：广东省科学技术协会科技交流部
- 页数：7
- 文件大小：1175k
- 原文格式：O
- 会议级别：地方
摘要
人参复合多糖(Ginseng polysaccharides complex,GPS)是从中国传统中草药人参中提取的多糖复合物,具有增强机体免疫力、降血糖、防辐射、抗感染等功效。本文以哺乳仔猪为研究对象,探讨人参复合多糖对仔猪的免疫调节作用。试验选取7日龄的哺乳仔猪8窝,随机分成4组,每组2窝,分别在基础饲粮中添加100 mg/kg、200 mg/kg和400 mg/kg人参复合多糖。结果表明,添加200 mg/kg人参复合多糖可以显著提高仔猪血清中圆环病毒抗体水平(P<0.05)。添加200 mg/kg和400 mg/kg人参复合多糖组除第7天外,在其余检测时间点均显著促进仔猪血液白细胞中TNF-α和IL-6的mRNA表达水平(P<0.05)。添加200mg/kg人参复合多糖42天时能显著提高仔猪血清中GSH-Px的活性(P<0.05),猪血清中MDA的含量在第14天、42天和56天显著低于对照组(P<0.05)。同时,本实验表明合适剂量GPS对猪圆环病毒疫苗的免疫效果有明显的增强作用,同时有抗氧化作用。
Ginseng polysaccharides complex(GPS) is extracted from traditional Chinese herb ginseng.Many researches indicated that polysaccharides extracted from Chinese herb had the ability to promote the immunity of bodies,and exert anti-virus as well.In this study,the immune stimulating effect of GPS on piglets was therefore investigated in vitro.Seven days old suckling piglets from 8 litters were divided into 4 groups,2 litters per group.The pigs were fed basal diet containing 100mg/kg,200mg/kg and 400mg/kg GPS respectively,adopting continuous feeding freely.The results showed that 200mg/kg GPS increased the porcine circovirus virus antibody level significantly(P<0.05) and 200mg/kg and 400mg/kg GPS promoted the mRNA expression of TNF-a and IL-6 in white blood cells significantly at all time points except on day 7(P<0.05).200mg/kg GPS significantly increased the activity of GSH-Px in serum on day 42(P < 0.05).200mg/kg GPS significantly reduced the MDA concentration in serum at day 14,42 and 56(P<0.05).These findings indicated that the GPS possessed a significant immune enhancing on PCV vaccines in piglets,and increased antioxidant ability of pigs.
Ginseng polysaccharides complex(GPS) is extracted from traditional Chinese herb ginseng.Many researches indicated that polysaccharides extracted from Chinese herb had the ability to promote the immunity of bodies,and exert anti-virus as well.In this study,the immune stimulating effect of GPS on piglets was therefore investigated in vitro.Seven days old suckling piglets from 8 litters were divided into 4 groups,2 litters per group.The pigs were fed basal diet containing 100mg/kg,200mg/kg and 400mg/kg GPS respectively,adopting continuous feeding freely.The results showed that 200mg/kg GPS increased the porcine circovirus virus antibody level significantly(P<0.05) and 200mg/kg and 400mg/kg GPS promoted the mRNA expression of TNF-a and IL-6 in white blood cells significantly at all time points except on day 7(P<0.05).200mg/kg GPS significantly increased the activity of GSH-Px in serum on day 42(P < 0.05).200mg/kg GPS significantly reduced the MDA concentration in serum at day 14,42 and 56(P<0.05).These findings indicated that the GPS possessed a significant immune enhancing on PCV vaccines in piglets,and increased antioxidant ability of pigs.
引文