正常与高血脂家兔全身动脉铸型腐蚀方法的比较

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• 英文论文题名：Comparison of the methods of systemic arterial infusion in normal and hyperlipidemic rabbits
• 论文作者：殷远行 ; 申彪 ; 张积霞 ; 陈施彤 ; 李泽坤
• 英文论文作者：YIN Yuan-hang ; SHEN Biao ; CHEN Shi-tong ; LI Ze-kun ; Xinxiang Medical University ; College of pharmacy ; Xinxiang Medical University
• 年：2017
• 作者机构：新乡医学院;新乡医学院药学院;
• 论文关键词：家兔 ; 高血脂 ; 血管铸型 ; 混合腐蚀法
• 英文论文关键词：rabbit ; hyperlipidemia ; vascular cast ; the method of mixed corrosion method
• 会议召开时间：2017-08-07
• 会议录名称：中国解剖学会第六届全国解剖学技术学术会议论文汇编
• 语种：中文
• 分类号：R-332;R589.2
• 学会代码：ZGJP
• 会议名称：中国解剖学会第六届全国解剖学技术学术会议
• 会议地点：中国江西九江
• 主办单位：中国解剖学会科技开发和咨询工作委员会
• 学会名称：中国解剖学会
• 页数：3
• 文件大小：1985k
• 原文格式：D
• 会议级别：全国

摘要

目的比较正常家兔与高血脂家兔全身动脉铸型的不同,为临床研究和诊治高血脂疾病提供形态学资料。方法从家兔左心室开始插管到冠状动脉升主动脉结束并结扎,将填充剂从插管注入,待填充剂凝固后,采用新的创新技术"水煮法"结合"酸腐蚀法"进行腐蚀,去除骨骼和有机软组织,得到其血管铸型。用高脂配方喂养家兔,建造高血脂动物模型,按照正常家兔血管铸型的方法得到病理家兔血管铸型。观察正常与高血脂家兔大体形态结构血管构筑和组织结构。结果 1.正常家兔的全身动脉血管铸型显示:血管粗且光滑,填充剂灌注均匀;高血脂家兔的全身动脉血管铸型血管细且粗糙,灌注不均匀,有部分血管没有被显示。2.组织切片显示:正常家兔的主动脉膜结构完整,内皮光滑且内皮处没有异物沉积。高血脂家兔主动脉内膜粗糙且增厚,内皮处有大量的泡沫细胞。结论混合腐蚀法(水煮法结合酸腐蚀法)所制作的家兔动脉铸型比较清晰完整,是较好的制作血管铸型的技术方法。
Objective To compare the difference between the normal rabbits' and the hyperlipidemia rabbts ' the whole body arterial casting mould,and to provide morphological data for clinical research and diagnosis and treatment of hyperlipidemia.Methods The cannula from rabbit left ventricle to the coronary artery into the ascending aorta and end,ligated,filler is injected from the cannula,solidified,the new technology innovation "water-boiling method " combined with acid etching method were used for corroding,removal of bone and soft tissue.There were their vascular casts.The rabbits were fed with high fat diet and the animal model of hyperlipidemia was established,and then the blood vessels were obtained according to the normal rabbit blood vessel technique.To observe the difference of vascular intima and morphology between normal and hyperlipemia rabbits.Results 1.The arterial blood vessels of normal rabbits showed that the vessels were thick and smooth,uniform filling.The arterial blood vessels of rabbits with high blood fat were fine and rough,perfusion wes not uniform,some blood vessels were not filled with filler.2.Histological sections showed that the membrane structure of rabbit aorta was intact,the endothelium wes smooth and there wes no foreign body deposition in the endothelium.The tunica intima of aorta in rabbits with hyperlipemia was rough and thick,and there were a large number of foam cells.Conclusion The method of mixed corrosion method(boiling method combined with acid etching method) is clearer and more complete,and it is a good method to make blood vessel cast.

引文

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