鸡心包积液-肝炎综合征病原初步研究
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- 英文论文题名：Preliminary studies on chicken pericardial effusion and hepatitis syndrome
- 论文作者：袁万哲 ; 张姗 ; 陈萍 ; 崔元 ; 经美 ; 李佳暖 ; 左奕 ; 刘聚祥 ; 孙继国
- 英文论文作者：YUAN Wanzhe ; LI Yubao ; WANG Jichang ; ZHANG Shan ; CHEN Ping ; CUI Yuan ; JING Mei ; LI Jiannuan ; ZUO Yi ; DONG Shishan ; CHEN Ligong ; LIU Juxiang ; SUN Ji-guo ; College of Animal Medicine ; Agricultural University of Hebei
- 年：2016
- 作者机构：河北农业大学动物医学院;
- 论文关键词：鸡心包积液-肝炎综合征 ; 肉鸡 ; 禽腺病毒4型
- 英文论文关键词：Chicken pericardial effusion and hepatitis syndrome(CPEHS) ; broiler ; fowl adenovirus 4
- 会议召开时间：2016-07-20
- 会议录名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会论文集
- 语种：中文
- 分类号：S858.31
- 学会代码：SYGW
- 会议名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会
- 会议地点：中国内蒙古通辽
- 主办单位：中国畜牧兽医学会兽医公共卫生学分会
- 学会名称：中国畜牧兽医学会兽医公共卫生学分会
- 页数：6
- 文件大小：728k
- 原文格式：D
- 会议级别：全国
摘要
2015年6月以来,我国部分地区所饲养的肉鸡与蛋鸡发生一种疾病,以心包积液,肝炎为特点,根据病变,暂将该病称为鸡心包积液-肝炎综合征。从不同地方的发病鸡群采集20份病料,发病鸡均能检出禽腺病毒,将阳性病料接种9日龄SPF鸡胚,获得1株禽腺病毒分离株SDSX1。用SDSX1株的hexon基因序列进行分析表明,SDSX1株与禽腺病毒4型具有相近的遗传进化关系,用其感染1日龄SPF鸡,可复制出心包积液,肝炎的病变。结果表明,从鸡心包积液-肝炎自然病例分离到的一种禽腺病毒可能与鸡心包积液-肝炎综合征有关。
From June in 2015,a disease characterized by pericardial effusion and hepatitis was observed in many broiler and egg-laying farm in China.Based on the pathological changes,the disease was tentatively called chicken pericardial effusion and hepatitis syndrome.Twenty samples were collected from the diseased chicken in different regions and were subjected to virus isolation using SPF chicken embryos.One isolate was obtained from livers.PCR results showed that the isolate was positive for fowl adenovirus,designated SDSX1.The isolate was further investigation.Sequence analysis based on a 743 bp hexon gene revealed that strain SDSX1 was most closely related to fowl adenovirus 4(FAV-4).Experimental infections resulted in pericardial effusion and hepatitis.The results suggested that FAV-4 was isolated from the chicken pericardial effusion and hepatitis syndrome(CPEHS) cases,which may be caused by FAV-4 infection.
From June in 2015,a disease characterized by pericardial effusion and hepatitis was observed in many broiler and egg-laying farm in China.Based on the pathological changes,the disease was tentatively called chicken pericardial effusion and hepatitis syndrome.Twenty samples were collected from the diseased chicken in different regions and were subjected to virus isolation using SPF chicken embryos.One isolate was obtained from livers.PCR results showed that the isolate was positive for fowl adenovirus,designated SDSX1.The isolate was further investigation.Sequence analysis based on a 743 bp hexon gene revealed that strain SDSX1 was most closely related to fowl adenovirus 4(FAV-4).Experimental infections resulted in pericardial effusion and hepatitis.The results suggested that FAV-4 was isolated from the chicken pericardial effusion and hepatitis syndrome(CPEHS) cases,which may be caused by FAV-4 infection.
引文
[1]袁万哲,邹云婧,孙继国,等.禽流感病毒与新城疫病毒二重RT-PCR检测方法的建立[J].河北农业大学学报,2015,27(6):296-299.
[2]扈荣良.现代动物病毒学[M].中国农业出版社,2014,681-683.
[3]Grgic H,Poljak Z,Sharif S,et al.Pathogenicity and cytokine gene expression pattern of a serotype 4 fowl adenovirus isolate[J].PLo S One,2013,8,e77601.
[2]扈荣良.现代动物病毒学[M].中国农业出版社,2014,681-683.
[3]Grgic H,Poljak Z,Sharif S,et al.Pathogenicity and cytokine gene expression pattern of a serotype 4 fowl adenovirus isolate[J].PLo S One,2013,8,e77601.