9种内源酶对南极磷虾中氟迁移的影响
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- 英文论文题名：The effect of nine endogenous enzymes on migration of fluoridein euphausia superba
- 论文作者：吴晶 ; 朱兰兰 ; 郭彤 ; 周德庆
- 英文论文作者：Wu Jing ; Zhu Lanlan ; Guo Tong ; Zhou Deqing ; College of Food Science and Technology ; Shanghai Ocean University ; Yellow Sea Fishery Research Institute Chinese Academy of Fishery Science ; College of Food Science and Technology ; Ocean University of China
- 年：2016
- 作者机构：上海海洋大学食品学院;中国水产科学研究院黄海水产研究所;中国海洋大学食品科学与工程学院;
- 论文关键词：南极磷虾 ; 氟的迁移 ; 内源酶
- 英文论文关键词：euphausia superba ; migration of fluoride ; endogenous enzymes
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS254.1
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：110k
- 原文格式：O
- 会议级别：全国
摘要
大量研究表明,南极磷虾在自溶过程中伴随着氟的不可逆迁移,而南极磷虾体内大量的内源酶对自溶产生重要影响。为研究南极磷虾体内内源酶对氟迁移的影响,以胃蛋白酶、羧肽酶A、甘氨酸羧肽酶、胰凝乳蛋白酶、胰蛋白酶、碱性磷酸酶、羧酸酯酶、胆碱酯酶、芳香基硫酸酯酶9种酶为研究对象,通过添加相应抑制剂来抑制酶活性,探讨9种内源酶对南极磷虾氟迁移的影响。结果表明,羧肽酶A、胰凝乳蛋白酶与虾壳中氟的溶出呈显著正相关趋势;胃蛋白酶、甘氨酸羧肽酶、胰蛋白酶、碱性磷酸酶、胆碱酯酶与虾壳中氟的溶出呈显著负相关趋势;胃蛋白酶、羧肽酶A与虾肉中氟的溶入呈显著正相关趋势;甘氨酸羧肽酶、胰凝乳蛋白酶、碱性磷酸酶、羧酸酯酶、胆碱酯酶与虾肉中氟的溶入呈显著负相关趋势。9种内源酶对南极磷虾中氟迁移的作用效果不一。
A large study showed that the fluorine irreversible migration of Antarctic krill in the process of autolysis.Plenty of endogenous enzymes playan important role inthe process of autolysis.Nine endogenous enzymeswere selected to study the effects of different endogenous enzymes on migration of fluoride,such as pepsin,carboxypeptidase A,Glycine carboxypeptidase,chymotrypsin,trypsin,alkaline phosphatase,carboxylesterase,cholinesterase,arylsulphatase.To inhibit enzyme activity,different inhibitors were added into Antarctic krill.The results showed that the dissolution of fluoride in krill carapacehada significant tendency of positive correlationwith carboxypeptidase A and chymotrypsin.The dissolution of fluoride in krill carapacehada significant tendency of negative correlationwith pepsin,Glycine carboxypeptidase,trypsin,alkaline phosphatase,cholinesterase.Compared with blank group,the integration of fluoride in krill musclehada significant tendency of positive correlationwith pepsin and carboxypeptidase A.Compared with blank group,the integration of fluoride in krill musclehada significant tendency of negative correlationwithglycine carboxypeptidase,chymotrypsin,alkaline phosphatase,carboxylesterase and cholinesterase.Nine endogenous enzymes had different effects on migration of fluoride in Antarctic krill.
A large study showed that the fluorine irreversible migration of Antarctic krill in the process of autolysis.Plenty of endogenous enzymes playan important role inthe process of autolysis.Nine endogenous enzymeswere selected to study the effects of different endogenous enzymes on migration of fluoride,such as pepsin,carboxypeptidase A,Glycine carboxypeptidase,chymotrypsin,trypsin,alkaline phosphatase,carboxylesterase,cholinesterase,arylsulphatase.To inhibit enzyme activity,different inhibitors were added into Antarctic krill.The results showed that the dissolution of fluoride in krill carapacehada significant tendency of positive correlationwith carboxypeptidase A and chymotrypsin.The dissolution of fluoride in krill carapacehada significant tendency of negative correlationwith pepsin,Glycine carboxypeptidase,trypsin,alkaline phosphatase,cholinesterase.Compared with blank group,the integration of fluoride in krill musclehada significant tendency of positive correlationwith pepsin and carboxypeptidase A.Compared with blank group,the integration of fluoride in krill musclehada significant tendency of negative correlationwithglycine carboxypeptidase,chymotrypsin,alkaline phosphatase,carboxylesterase and cholinesterase.Nine endogenous enzymes had different effects on migration of fluoride in Antarctic krill.
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