诊疗一体化的多功能纳米基因载体构建
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- 英文论文题名：Construction of Multifunctional Nanoparticle-Based Gene Carriers for Theranostics
- 论文作者：赵娜娜 ; 王冉冉 ; 杨亚娟 ; 徐福建
- 英文论文作者：Nana Zhao ; Ranran Wang ; Yajuan Yang ; Fu-Jian Xu ; College of Materials Science & Engineering ; Beijing University of Chemical Technology
- 年：2016
- 作者机构：北京化工大学材料科学与工程学院;
- 论文关键词：无机纳米颗粒 ; 功能化 ; 基因载体 ; 形貌 ; 成像
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十八分会：纳米生物效应与纳米药物化学
- 语种：中文
- 分类号：TB383.1;TQ421.7
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十八分会 ; 纳米生物效应与纳米药物化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：208k
- 原文格式：D
- 会议级别：全国
摘要
基因治疗在癌症等重大疾病的治疗方面有巨大的潜力,而研发和制备低毒、高效的基因载体仍是基因治疗中很大的挑战。本工作以具有独特物理、化学性质的量子点、氧化铁和金等无机纳米颗粒为模型,发展了制备阳离子聚合物功能化的有机/无机复合纳米基因载体的有效方法;所得特殊形貌的纳米基因载体表现出高效的基因转染效率和较低的生物毒性,并且兼具优异的荧光、CT和核磁造影成像功能,在同一纳米结构中实现了诊疗一体化(图1中以量子点氧化硅核壳纳米颗粒为例)。这种复合纳米基因载体的设计和制备为多功能基因/药物传输系统的构建提供了新的思路,也将有利于研究纳米材料与生物体系的相互作用。
Gene therapy provides a promising way to cure cancer and genetic disorders while the development of efficient and safe gene vectors is still the main challenge. In this work, a facile strategy to functionalize quantum dots, iron oxide, and gold nanoparticles with unique physical and chemical properties to fabricate organic/inorganic gene carriers is developed. The obtained gene carriers with special morphologies demonstrate high gene transfection efficiency and low cytotoxicity. In addition, the potential of the gene carriers to be utilized as fluorescence, CT and MR contrast agents for imaging has also been displayed. Therefore, the integration of efficient gene delivery and real-time imaging could be realized within one nanostructure(quantum dots@SiO_2 as an example in Fig. 1). The design and synthesis of nanocomposite gene carriers provide a new avenue for multifunctional gene/drug delivery and gain useful information on the interaction of nanomaterials with biological systems.
Gene therapy provides a promising way to cure cancer and genetic disorders while the development of efficient and safe gene vectors is still the main challenge. In this work, a facile strategy to functionalize quantum dots, iron oxide, and gold nanoparticles with unique physical and chemical properties to fabricate organic/inorganic gene carriers is developed. The obtained gene carriers with special morphologies demonstrate high gene transfection efficiency and low cytotoxicity. In addition, the potential of the gene carriers to be utilized as fluorescence, CT and MR contrast agents for imaging has also been displayed. Therefore, the integration of efficient gene delivery and real-time imaging could be realized within one nanostructure(quantum dots@SiO_2 as an example in Fig. 1). The design and synthesis of nanocomposite gene carriers provide a new avenue for multifunctional gene/drug delivery and gain useful information on the interaction of nanomaterials with biological systems.
引文
[1]Zhao,N.;Lin,X.;Zhang,Q.;Ji,Z.;Xu,F.J.Small 2015,11:6467.
[2]Yan,P.;Wang,R.;Zhao,N.;Zhao,H.;Chen,D.F.;Xu,F.J.Nanoscale 2015,7:5281.
[3]Yan,P.;Zhao,N.;Hu,H.;Lin,X.;Liu,F.;Xu,F.J.Acta Biomater.2014,10:3786.
[2]Yan,P.;Wang,R.;Zhao,N.;Zhao,H.;Chen,D.F.;Xu,F.J.Nanoscale 2015,7:5281.
[3]Yan,P.;Zhao,N.;Hu,H.;Lin,X.;Liu,F.;Xu,F.J.Acta Biomater.2014,10:3786.