一种基于超顺磁性纳米粒子的磁弛豫时间适配体传感器检测铜绿假单胞菌
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- 英文论文题名：An MRSw aptasensor for the rapid detection of Pseudomonas aeruginosa using superparamagnetic nanoparticles
- 论文作者：贾飞 ; 徐雷 ; 闫文杰 ; 邬威 ; 喻倩倩 ; 田晓静 ; 戴瑞彤 ; 李兴民
- 英文论文作者：Jia Fei ; Xu Lei ; Yan Wenjie ; Wu Wei ; Yu Qianqian ; Tian Xiaojing ; Dai Ruitong ; Li Xingmin ; College of Food Science and Nutritional Engineering ; China Agricultural University ; College of Applied Arts and Science ; Beijing Union University
- 年：2016
- 作者机构：中国农业大学食品科学与营养工程学院;北京联合大学应用文理学院;
- 论文关键词：磁弛豫时间 ; 超顺磁性纳米材料 ; 适配体传感器 ; 快速检测 ; 铜绿假单胞菌
- 英文论文关键词：magnetic relaxation switch ; superparamagnetic iron oxide(SPIO) ; aptasensor ; rapid detection ; pseudomonas aeruginos
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS207.4
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：1
- 文件大小：62k
- 原文格式：O
- 会议级别：全国
摘要
本文构建了一种磁弛豫时间适配体传感器快速检测铜绿假单胞菌。本传感器基于适配体识别技术和磁弛豫时间原理。首先利用酰胺反应将铜绿假单胞菌适配体共价结合于超顺磁性纳米材料表面。超顺磁性纳米材料的磁放大能力和适配体识别体系的高亲和力保证检测方法的灵敏度和选择性。在铜绿假单胞菌菌存在条件下,适配体能特异性将铜绿假单胞菌菌捕获,引起超顺磁性纳米粒子的聚集,从而导致传感器中弛豫时间T_2的变化。根据弛豫时间T_2的变化实现对铜绿假单胞菌的定量检测。在优化条件下,T_2的变化差值与铜绿假单胞菌菌菌液浓度的对数值成线性关系,线性范围为10~2cfu/mL~10~6cfu/mL,检测限为50cfu/mL(S/N\=3)。将构建的适配体传感器用于食品和饮用水中铜绿假单胞菌的检测,并用平板计数法进行验证,证明本方法可用于实际样本的检测。同时,本文构建的传感器灵敏度高、造价低廉、简便易用,能够应用于其他食源性致病菌的检测当中。
A simple,rapid and sensitive magnetic relaxation switch(MRSw) aptasensor was developed for the detection of Pseudomonas aeruginosa.This aptasensor is based on an aptamer-recognition system and magnetic relaxation switch assay.The anti-P.aeruginosa aptamer was covalently bound to the superparamagnetic iron oxide(SPIO) nanoparticles through amide bonds.The magnetic amplification of SPIO nanoparticles and the high affinity of the aptamer-recognition system ensured the highly sensitivity and selectivity of the assay.When incubated with P.aeruginosa,the aptamer selectively captured target bacteria and hence affected the formation of SPIO aggregates,resulting in a dramatic increase in the spin-spin relaxation time(T_2),which can be utilized to quantify the bacteria.Under optimized conditions,the aptasensor possessed a linear range of10~2cfu/mL to 10~6cfu/mL and a detection limit of 50cfu/mL(S/N = 3).The proposed method was then applied to detect P.aeruginosa in real food and drinking water samples and was validated by the traditional plate counting method.The results showed that the MRSw aptasensor is a promising platform for the determination of P.aeruginosa and could be extended to other food-borne bacteria or biomolecules due to its high sensitivity,low-cost,simplicity and ease of use.
A simple,rapid and sensitive magnetic relaxation switch(MRSw) aptasensor was developed for the detection of Pseudomonas aeruginosa.This aptasensor is based on an aptamer-recognition system and magnetic relaxation switch assay.The anti-P.aeruginosa aptamer was covalently bound to the superparamagnetic iron oxide(SPIO) nanoparticles through amide bonds.The magnetic amplification of SPIO nanoparticles and the high affinity of the aptamer-recognition system ensured the highly sensitivity and selectivity of the assay.When incubated with P.aeruginosa,the aptamer selectively captured target bacteria and hence affected the formation of SPIO aggregates,resulting in a dramatic increase in the spin-spin relaxation time(T_2),which can be utilized to quantify the bacteria.Under optimized conditions,the aptasensor possessed a linear range of10~2cfu/mL to 10~6cfu/mL and a detection limit of 50cfu/mL(S/N = 3).The proposed method was then applied to detect P.aeruginosa in real food and drinking water samples and was validated by the traditional plate counting method.The results showed that the MRSw aptasensor is a promising platform for the determination of P.aeruginosa and could be extended to other food-borne bacteria or biomolecules due to its high sensitivity,low-cost,simplicity and ease of use.
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