新型皮质醇电化学传感器研究
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- 英文论文题名：Investigate a novel electrochemical sensor of cortisol
- 论文作者：孙伯禄 ; 胡芳弟
- 英文论文作者：Bolu Sun ; Fangdi Hu ; School of Pharmacy ; Lanzhou University
- 年：2016
- 作者机构：兰州大学药学院;
- 论文关键词：石墨烯 ; 电化学传感器 ; 皮质醇
- 英文论文关键词：Graphene ; Electrochemical sensor ; Cortisol(Cor)
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十九分会：电化学材料
- 语种：中文
- 分类号：O657.1;TP212.2
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十九分会 ; 电化学材料
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：310k
- 原文格式：D
- 会议级别：全国
摘要
构建了一种基于金纳米粒子(AuNPs)/Fe_3O_4磁功能化石墨烯(MrGO)的竞争型皮质醇电化学免疫传感器。首先通过化学交联的方法成功制备了MrGO,并将其用于Nafion预处理的玻碳电极的修饰,接着通过电化学沉积的方法将AuNPs修饰在电极表面,借助AuN Ps/MrG O较大的比表面积和良好的生物相容性大量牢固的负载皮质醇(Cor)形成Cor/AuNPs/MrGO/Nafion@GCE基础电极,采用交流阻抗法和循环伏安法对基础电极的构建过程进行了表征。由于固定在电极表面的Cor与待测样品中的Cor能竞争性的与辣根过氧化物酶标记的皮质醇抗体(HRP-Strept-Biotin-Ab)特异性结合,而形成的HRP-Strept-Biotin-Ab-Cor/AuNPs/MrGO@Nafion/GCE免疫传感器在测试底液中的电化学响应与样品中Cor浓度呈负相关性。AuNPs/MrGO的高导电性使得构建的传感器的电化学响应得到了极大的增强,并具有良好的精密度和较好的稳定性。在优化的条件下,实现了人血浆皮质醇的灵敏、高效、实时的检测,也为临床皮质醇的检测建立了一种新颖便捷的检测方法。
A sensitively competitive electrochemical immunosensor for the detection of cortisol was successfully developed based on gold nanoparticles and magnetic functionalized reduced graphene oxide(AuN Ps/MrGO). The immunosensor design involves the combination of MrGO-modified nafion pretreated glassy carbon electrodes and electrochemical deposition of AuN Ps, to efficiently immobilize cortisol, with a competitive immunoassay involving biotinylated cortisol antibody(Biotin-Cor), and a differential pulse voltammetric detection step using horseradish peroxidase-labelled streptavidin(HRP-Strept) and test liquid. The AuN Ps/MrGO with excellent electrical conductivity being applied, the electrochemical response of the immunosensor was immensely amplified. Moreover, the proposed method showed good precision, acceptable stability and reproducibility, and could be used for the sensitive, efficient and real-time detection of cortisol in real samples, which provides a novel and convenient method for clinical determination of cortisol.
A sensitively competitive electrochemical immunosensor for the detection of cortisol was successfully developed based on gold nanoparticles and magnetic functionalized reduced graphene oxide(AuN Ps/MrGO). The immunosensor design involves the combination of MrGO-modified nafion pretreated glassy carbon electrodes and electrochemical deposition of AuN Ps, to efficiently immobilize cortisol, with a competitive immunoassay involving biotinylated cortisol antibody(Biotin-Cor), and a differential pulse voltammetric detection step using horseradish peroxidase-labelled streptavidin(HRP-Strept) and test liquid. The AuN Ps/MrGO with excellent electrical conductivity being applied, the electrochemical response of the immunosensor was immensely amplified. Moreover, the proposed method showed good precision, acceptable stability and reproducibility, and could be used for the sensitive, efficient and real-time detection of cortisol in real samples, which provides a novel and convenient method for clinical determination of cortisol.
引文
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[2]O’Connor D B,Ferguson E,Green J A,et al.Psychoneuroendocrinology,2016,63:370-379.
[2]O’Connor D B,Ferguson E,Green J A,et al.Psychoneuroendocrinology,2016,63:370-379.