安徽省烟草根腐病病原鉴定及生物学特性研究
详细信息 查看官网全文
- 英文论文题名：Identification and Characterization of Tobacco Root Rot Disease Pathogens in Anhui Province
- 论文作者：江寒 ; 叶磊 ; 王文凤 ; 檀根甲
- 英文论文作者：JIANG Han ; YE Lei ; WANG Wen-feng ; TAN Gen-jia ; School of Plant Protection ; Anhui Agricultural University
- 年：2016
- 作者机构：安徽农业大学植物保护学院;
- 论文关键词：烟草 ; 根腐病 ; 病原菌 ; 生物学特性
- 英文论文关键词：Tobacco ; Root Rot ; Pathogens ; Biological characteristics
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：中文
- 分类号：S435.72
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：10
- 文件大小：1796k
- 原文格式：O
- 会议级别：全国
摘要
烟草是重要的经济作物,其质量和产量受到烟草病虫害等一些重要因素的影响,烟草根腐病是农业生产上的一种重要根部病害,而耕作制度和栽培方式的改变,更是加重了该病害的发生。本文从烟草根腐病病样根部,采用常规组织分离法分离并纯化获得两种病原,通过形态学和分子生物学方法鉴定并确定两种病原菌分别为尖孢镰刀菌(Fusarium oxysporum)和黄色镰刀菌(Fusarium culmorum),并探究了两种病原菌的生物学特性,结果表明:两种病原菌菌丝生长和孢子萌发的最适温度分别是25℃及30℃,最适pH值都是7;两种病原菌菌丝生长最适碳氮源都分别为蔗糖和硝酸钾;尖孢镰刀菌的致死温度为55℃,黄色镰刀菌则为60℃。其结果为烟草根腐病的综合绿色防治提供了科学理论依据.
Tobacco is an important economic crop,one of the factors that infect the quality and yield of tobacco were diseases and insect pest.Tobacco root rot was one kind of root and stem diseases.The adjustment of planting structure aggravates the degree of this disease.From the selected sample root of Tobacco root rot,two pathogens isolated by tissue separation method was obtained.The pathogens were identified morphologically and molecularlly as Fusarium oxysporum and Fusarium culmorum,the biological characteristics of the pathogens was studied.The results indicated that the most suitable temperature for the mycelial growth and spore germination is 25 ℃ and 30 ℃ respectively.The most suitable for mycelia growth,spore germination of pH is 7.The optimum carbon and nitrogen source were sucrose and potassium nitrate.The lethal temperature for Fusarium oxysporum is 55 ℃,for Fusarium culmorum is60℃.The finding shows theoretical basis for the control of tobacco root rot.
Tobacco is an important economic crop,one of the factors that infect the quality and yield of tobacco were diseases and insect pest.Tobacco root rot was one kind of root and stem diseases.The adjustment of planting structure aggravates the degree of this disease.From the selected sample root of Tobacco root rot,two pathogens isolated by tissue separation method was obtained.The pathogens were identified morphologically and molecularlly as Fusarium oxysporum and Fusarium culmorum,the biological characteristics of the pathogens was studied.The results indicated that the most suitable temperature for the mycelial growth and spore germination is 25 ℃ and 30 ℃ respectively.The most suitable for mycelia growth,spore germination of pH is 7.The optimum carbon and nitrogen source were sucrose and potassium nitrate.The lethal temperature for Fusarium oxysporum is 55 ℃,for Fusarium culmorum is60℃.The finding shows theoretical basis for the control of tobacco root rot.
引文
[1]陈芷,张绍升.福建四种烟草根茎病害诊断及其病原学研究[D].福州:福建农林大学,2012.
[2]胡语婕,冯斗.生物柴油树种石栗繁殖技术研究[D].南宁:广西大学,2010.
[3]李锡宏,许汝冰,黎妍妍,等.清江流域烟草病虫害发生与防治技术研究[J].中国烟草科学,2011,32(05):112-116.
[4]叶瑞强.戚益军.拟南芥AGO4/siRNA复合体细胞质内组装和不依赖DCL的小RNA介导DNA甲基化的机理研究[D].杭州:浙江大学,2014.
[5]费丹,檀根甲,罗道宏.安徽省水稻穗腐病病原鉴定及生物学特性研究[J].安徽农业大学学报,2014,41(5):777-782.
[6]窦彦霞,肖崇刚,蒋茜,等.重庆烟草根黑腐病菌的生物学特性[J].烟草科技,2009(11):56-60.
[7]王佳,杜春梅.大豆根腐病生防菌的鉴定及发酵条件的优化[D].哈尔滨:黑龙江大学,2010.
[8]杨继余,王娜,王立事.不同碳、氮源对榆白涩病病菌的影响[J].北方园艺.2012(19):153-154.
[9]蒋晓东,杨晓萍.茶叶提取物对桃软腐菌的抑制机理及其活体保鲜研究[D].武汉:华中农业大学,2014.
[10]陈高航,侯明生.烟草根腐病病原鉴定及其生物学特性观察[D].武汉:华中农业大学,2013.
[11]叶旭红,林先贵,王一明.尖孢镰刀菌致病相关因子及其分子生物学研究进展[J].应用与环境生物学报.2011,17(5):759-762.
[12]李红玫,蒋选利.贵州省草石蚕病害种类调查及其腐烂病化学防治技术的研究[D].贵阳:贵州大学.2010.
[13]何冬云,王生荣.玛曲草原土壤真菌分离鉴定及多样性研究[D].兰州:甘肃农业大学,2010.
[14]]赵杰,孔凡玉.山东省烟草镰刀菌根腐病病原及生物学特性的研究[D].北京:中国农业科学院.2013.
[15]Pietro A D,Madrid M P,Caracuel Z,et al.Fusarium oxysporum;exploring the molecular arsenal of a vascular wilt fungus[J].Molecular Plant Pathology,2003,4(5):315-325.
[2]胡语婕,冯斗.生物柴油树种石栗繁殖技术研究[D].南宁:广西大学,2010.
[3]李锡宏,许汝冰,黎妍妍,等.清江流域烟草病虫害发生与防治技术研究[J].中国烟草科学,2011,32(05):112-116.
[4]叶瑞强.戚益军.拟南芥AGO4/siRNA复合体细胞质内组装和不依赖DCL的小RNA介导DNA甲基化的机理研究[D].杭州:浙江大学,2014.
[5]费丹,檀根甲,罗道宏.安徽省水稻穗腐病病原鉴定及生物学特性研究[J].安徽农业大学学报,2014,41(5):777-782.
[6]窦彦霞,肖崇刚,蒋茜,等.重庆烟草根黑腐病菌的生物学特性[J].烟草科技,2009(11):56-60.
[7]王佳,杜春梅.大豆根腐病生防菌的鉴定及发酵条件的优化[D].哈尔滨:黑龙江大学,2010.
[8]杨继余,王娜,王立事.不同碳、氮源对榆白涩病病菌的影响[J].北方园艺.2012(19):153-154.
[9]蒋晓东,杨晓萍.茶叶提取物对桃软腐菌的抑制机理及其活体保鲜研究[D].武汉:华中农业大学,2014.
[10]陈高航,侯明生.烟草根腐病病原鉴定及其生物学特性观察[D].武汉:华中农业大学,2013.
[11]叶旭红,林先贵,王一明.尖孢镰刀菌致病相关因子及其分子生物学研究进展[J].应用与环境生物学报.2011,17(5):759-762.
[12]李红玫,蒋选利.贵州省草石蚕病害种类调查及其腐烂病化学防治技术的研究[D].贵阳:贵州大学.2010.
[13]何冬云,王生荣.玛曲草原土壤真菌分离鉴定及多样性研究[D].兰州:甘肃农业大学,2010.
[14]]赵杰,孔凡玉.山东省烟草镰刀菌根腐病病原及生物学特性的研究[D].北京:中国农业科学院.2013.
[15]Pietro A D,Madrid M P,Caracuel Z,et al.Fusarium oxysporum;exploring the molecular arsenal of a vascular wilt fungus[J].Molecular Plant Pathology,2003,4(5):315-325.