能够特异性捕获-释放细菌的生物活性表面
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- 英文论文题名：Bioactive surfaces with capability to specifically capture and release bacteria
- 论文作者：渠阳翠 ; 韦婷 ; 占文俊 ; 胡昌明 ; 曹利敏 ; 于谦 ; 陈红
- 英文论文作者：Yangcui Qu ; Ting Wei ; Wenjun Zhan ; Changming Hu ; Limin Cao ; Qian Yu ; Hong Chen ; College of Chemistry ; Chemical Engineering and Materials Science ; Soochow University Suzhou
- 年：2016
- 作者机构：苏州大学材料与化学化工学部;
- 论文关键词：层层组装 ; 主客体相互作用 ; 细菌捕获
- 英文论文关键词：layer-by-layer assembly ; host-guest interaction ; bacterial capture
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十一分会：胶体与界面化学
- 语种：中文
- 分类号：Q93;O631.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十一分会 ; 胶体与界面化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：206k
- 原文格式：D
- 会议级别：全国
摘要
实现材料表面对细菌的特异性捕获在生物检测领域具有重要的实用意义。在本工作中,我们结合层层组装技术和主客体相互作用构建了一种能够特异性捕获-释放细菌的生物活性表面。首先选择具有特异性识别I型鞭毛类大肠杆菌的α-D-甘露糖作为配体,通过点击化学将其固定到主体分子β-环糊精上。而后通过层层组装技术在氨基活化的金表面上交替沉积了含有金刚烷基团的聚丙烯酸共聚物和聚丙烯胺,从而得到含有客体分子金刚烷基团的聚电解质多层膜。最后利用主客体相互作用,将甘露糖分子修饰的β-环糊精引入到表面。通过多种表征手段证实了表面的成功制备。细菌黏附实验结果表明该表面可以高效特异性捕获大肠杆菌(E.coli)而对其他的菌种没有明显的识别作用。同时,利用十二烷基磺酸钠(SDS)溶液处理表面可以破坏主客体相互作用从而释放捕获的细菌,最终使表面恢复初始状态并可用于再次负载甘露糖分子修饰的β-环糊精。该表面在生物检测领域具有潜在的应用价值。
Surfaces with capability to specifically capture bacteria play an important role in biological detection area.In this work,bioactive surfaces that can specifically capture and release bacteria was fabricated via the combination of layer-by-layer assembly and host-guest interaction.First,α-D-mannose with specificity against type 1 fimbrial bacteria was immobilized into host molecule(β-cyclodextrin) by click chemistry.Moreover,the activated surface was alternately deposited with P(AA-co-Ada) and PAH to form the multilayer films including guest molecule(adamantane).Finally,β-cyclodextrin decorated with mannose ligand(CD-M) was incorporated into surface via host-guest interaction.The successful preparation of the surface was monitored by various methods.Results of bacterial adhesion assay showed the surface could capture E.coli with high specificity.Meanwhile,the captured bacteria could be released by dissociation of host-guest interaction using SDS.Moreover,the surface after dissociation of host-guest interaction could be incorporated fresh CD-M molecules repeatedly to realize surface regeneration and reusage.
Surfaces with capability to specifically capture bacteria play an important role in biological detection area.In this work,bioactive surfaces that can specifically capture and release bacteria was fabricated via the combination of layer-by-layer assembly and host-guest interaction.First,α-D-mannose with specificity against type 1 fimbrial bacteria was immobilized into host molecule(β-cyclodextrin) by click chemistry.Moreover,the activated surface was alternately deposited with P(AA-co-Ada) and PAH to form the multilayer films including guest molecule(adamantane).Finally,β-cyclodextrin decorated with mannose ligand(CD-M) was incorporated into surface via host-guest interaction.The successful preparation of the surface was monitored by various methods.Results of bacterial adhesion assay showed the surface could capture E.coli with high specificity.Meanwhile,the captured bacteria could be released by dissociation of host-guest interaction using SDS.Moreover,the surface after dissociation of host-guest interaction could be incorporated fresh CD-M molecules repeatedly to realize surface regeneration and reusage.
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