斑点叉尾鮰维氏气单胞菌的分离鉴定及其病理损伤研究
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- 英文论文题名：Isolation, identification and pathological lesion reaserch of Aeromonas veronii from channel catfish
- 论文作者：段亚佼 ; 黄小丽 ; 杜宗君 ; 汪开毓 ; 樊威 ; 段靖 ; 邓永强 ; 耿毅 ; 陈德芳
- 英文论文作者：DUAN Yajiao ; HUANG Xiaoli ; Du Zongjun ; WANG Kaiyu ; FAN Wei ; DUAN Jing ; DENG Yongqiang ; GENG Yi ; CHEN Defang ; College of Animal Scicence and Technology ; Sichuan Agricultural University ; College of Veterinary Medicine ; Sichuan Agricultural University ; Animal Disease Prevention and Control Center of Sichuan
- 年：2016
- 作者机构：四川农业大学动物科技学院水产系;四川农业大学动物医学院;四川省动物疫病预防控制中心;
- 论文关键词：斑点叉尾鮰 ; 维氏气单胞菌 ; 16S rRNA ; gyrB ; 病理损伤
- 英文论文关键词：Channel catfish ; Aeromonas veronii ; 16S rRNA ; gyrB gene ; Pathological lesion
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S943
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：560k
- 原文格式：D
- 会议级别：全国
摘要
为确定一起斑点叉尾鮰体表褪色及胃肠道炎症的病原,为斑点叉尾鮰肠炎的正确诊断及防治提供理论依据。从病鱼脾脏和肾脏分离到两株优势菌(DYJ0320-1、DYJ0320-2),检测分离菌的形态、生理生化特性,以腹腔注射的方式进行人工感染试验,同时对16S rRNA和gyrB基因序列测定(Genebank登录号分别为KU925194和KU925195)与系统发育分析,并进行药敏试验。两株菌的生理生化特性均与已报道的维氏气单胞菌(Aeromonas veronii)相一致;16S rRNA和gyrB基因序列测定将其鉴定为维氏气单胞菌。病鱼多个组织器官具有典型的病理变化,分别引起坏死性、出血性胃肠炎,出血性脾炎及间质性肾炎,坏死性、溃疡性皮炎。药敏结果显示该菌对恩诺沙星、头孢西丁及左氧氟沙星等药物敏感。导致斑点叉尾鮰本次体表褪色及胃肠道炎症的主要病原菌为维氏气单胞菌。
To identify the pathogens caused the faded skin and inflammation of the digestive tract in Channel catfish, and provide a theoretical basis on correct diagnosis and prevention of channel catfish enteritis. Two dominant bacteria(DYJ0320-1、DYJ0320-2)had been isolated from the kidney and spleen of diseased fish. They were identified as Pseudomonas putida based on morphology, biochemical, physiology characteristics and sequencing of 16 S rRNA and gyrase B subunit(gyrB) gene(Gene Bank accession number were KP693689 and KP693690 respectively).They were also proved to be the pathogen of the disease by artificial infection experiment.Histologically, Damaged organs characterized by hemorrhagic necrotizing inflammation of the digestive tract, hemorrhagic inflammation of the spleen and interstitial nephritis, necrotizing, and ulcerative dermatitis. The result of drug sensitivity test showed that the isolates were senitive to the Enrofloxacin, Cefoxitin, Azithromycin, Levofloxacin, but were resistant to Ampiciuin,Doxycycline, novobiocin, Amoxicillin, Rifarnpin. The isolates proved to be the pathogen of channel catfish enteritis and was identified as Aeromonas veronii.
To identify the pathogens caused the faded skin and inflammation of the digestive tract in Channel catfish, and provide a theoretical basis on correct diagnosis and prevention of channel catfish enteritis. Two dominant bacteria(DYJ0320-1、DYJ0320-2)had been isolated from the kidney and spleen of diseased fish. They were identified as Pseudomonas putida based on morphology, biochemical, physiology characteristics and sequencing of 16 S rRNA and gyrase B subunit(gyrB) gene(Gene Bank accession number were KP693689 and KP693690 respectively).They were also proved to be the pathogen of the disease by artificial infection experiment.Histologically, Damaged organs characterized by hemorrhagic necrotizing inflammation of the digestive tract, hemorrhagic inflammation of the spleen and interstitial nephritis, necrotizing, and ulcerative dermatitis. The result of drug sensitivity test showed that the isolates were senitive to the Enrofloxacin, Cefoxitin, Azithromycin, Levofloxacin, but were resistant to Ampiciuin,Doxycycline, novobiocin, Amoxicillin, Rifarnpin. The isolates proved to be the pathogen of channel catfish enteritis and was identified as Aeromonas veronii.
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