淀粉基微凝胶与蛋白质相互作用的研究
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- 英文论文题名：Study on the interactions between starch-based microgels and protein
- 论文作者：章宝 ; 陈寒青
- 英文论文作者：Zhang Bao ; Chen Hanqing ; School of Food Science and Technology ; Hefei University of Technology
- 年：2016
- 作者机构：合肥工业大学食品科学与技术学院;
- 论文关键词：淀粉 ; 微凝胶 ; 溶菌酶 ; 静电相互作用
- 英文论文关键词：starch ; microgel ; lysozyme ; electrostatic interactions
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.2
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：115k
- 原文格式：O
- 会议级别：全国
摘要
基于微凝胶与蛋白质之间的静电作用,实现微凝胶时模型蛋白(溶菌酶)的包埋与控释。结果表明,随取代度(DS)的增加,羧甲基淀粉微凝胶对模型蛋白的包埋量增大,随(三偏磷酸钠与羧甲基淀粉质量比)R_(STMP/CMS)和离子强度的增加,微凝胶对模型蛋白的包埋量减少;当体系pH为6时,微凝胶对模型蛋白的包埋量最大。在最优条件下得到的微凝胶对溶菌酶最大包埋量为6.92mg/mg。采用激光共聚焦显微镜观察以FITC标记的溶菌酶在微凝胶内部的分布情况,结果表明:在低R_(STMP/CMS)时,溶菌酶均一分布在微凝胶内部,随R_(STMP/CMS)增加,溶菌酶在微凝胶内部分布减少,并主要集中于微凝胶的表层;高DS微凝胶包埋的溶菌酶荧光强度高于低DS微凝胶,证明了高DS微凝胶对溶菌酶包埋量高于低DS微凝胶;采用FTIR、拉曼光谱技术,证明微凝胶包埋未对溶菌酶的二级结构造成影响。体外模拟胃肠液释放研究结果表明,目标微凝胶可作为溶菌酶载体应用于肠道靶向载体传递系统中,通过圆二色谱表征发现天然溶菌酶和从微凝胶中释放的溶菌酶的二级结构无明显差异,表明在肠道靶向载体传递系统中目标微凝胶对溶菌酶具有很好的保护作用。
The electrostatic interactions between the CMS microgel and protein molecules were evaluated,which provided a stable control of uptaking and releasing model proteins(lysozyme).As a result,the loading capacity of microgel increased with increasing DS while the R_(STMP/CMS) and ionic strength had a negative effect on the loading capacity.A maximal loading capacity of microgels was obtained at 6.92mg/mg.The lysozyme-microgel complex was identified by confocal laser scanning microscopy since the lysozyme was labeled with FITC.It indicated that the lysozyme distribution was rather homogeneous in the microgel with low R_(STMP/CMS).The lysozyme was distributed around the surface area when the microgels had high R_(STMP/CMS).As for the effect of DS on the loading capacity of microgels,which could be as a function of fluorescence intensity,an increase of the DS decreased the fluorescence intensity.Furthermore,FTIR and Raman spectrum analysis results suggested that the secondary structure of lysozyme remained unchanged when lysozyme-microgel complex was formed.The control release of lysozyme under in vitro gastrointestinal tract environment confirmed that microgels can be used as carried materials for lysozyme(model proteins).Circular dichroism spectrum analysis results suggested that microgels provided the lysozyme with potential protection in the gastrointestinal delivery system due to the unchanged structure observed in lysozyme.
The electrostatic interactions between the CMS microgel and protein molecules were evaluated,which provided a stable control of uptaking and releasing model proteins(lysozyme).As a result,the loading capacity of microgel increased with increasing DS while the R_(STMP/CMS) and ionic strength had a negative effect on the loading capacity.A maximal loading capacity of microgels was obtained at 6.92mg/mg.The lysozyme-microgel complex was identified by confocal laser scanning microscopy since the lysozyme was labeled with FITC.It indicated that the lysozyme distribution was rather homogeneous in the microgel with low R_(STMP/CMS).The lysozyme was distributed around the surface area when the microgels had high R_(STMP/CMS).As for the effect of DS on the loading capacity of microgels,which could be as a function of fluorescence intensity,an increase of the DS decreased the fluorescence intensity.Furthermore,FTIR and Raman spectrum analysis results suggested that the secondary structure of lysozyme remained unchanged when lysozyme-microgel complex was formed.The control release of lysozyme under in vitro gastrointestinal tract environment confirmed that microgels can be used as carried materials for lysozyme(model proteins).Circular dichroism spectrum analysis results suggested that microgels provided the lysozyme with potential protection in the gastrointestinal delivery system due to the unchanged structure observed in lysozyme.
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