3-OH-BDE47对两栖动物的TR信号干扰效应
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- 英文论文题名：3-OH-BDE47 disrupts amphibians development via TR Signaling Pathway
- 论文作者：贾亚丽 ; 秦占芬
- 英文论文作者：Yali Jia ; Zhanfen Qin ; Research Center for Eco-Environmental Sciences ; Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院生态环境研究中心;
- 论文关键词：羟基多溴联苯醚 ; TH信号 ; 非洲爪蟾
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十六分会：环境化学
- 语种：中文
- 分类号：X503.22
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十六分会 ; 环境化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：166k
- 原文格式：D
- 会议级别：全国
摘要
作为一类新型的溴代有机污染物,OH-PBDEs已在多个营养级别的生物体内被广泛检测出,包括人类。近年来,研究发现OH-PBDEs会干扰动物甲状腺激素系统的正常生理功能。本研究以非洲爪蟾为模式生物,评价化合物3-OH-BDE47对TR信号通路的干扰效应。对52期蝌蚪采用短期6天的T3与3-OH-BDE47共暴露,从形态学和组织切片学分析蝌蚪在T3诱导的变态下,肠和脑组织整体形态的影响。利用RT-PCR分析不同暴露组蝌蚪的基因表达水平,从分子水平研究其对TR信号通路的影响。暴露自然变态的58期蝌蚪,进一步验证化学品的干扰作用。实验现象发现,高浓度的3-OH-BDE47对T3诱导发育的过程有促进作用,同时促进T3对有关TH响应基因(MMP13、TH/bzip、BTEB、Dio3)的表达。
As a new organic pollutants,OH-PBDEs is widely detected in many trophic level organisms,including human.In the rescent years,OH-PBDEs was reported to interefere with the THs fuction.In this study,we evaluated the effect of 3-OH-BDE47 on TR signaling pathway in Xenopus Laevis,what is a model organism.The tadpoles at stage 52 from a clutch were exposed to a series of concentrations of 3-OH-BDE47 in the presence of T3 for 6 days.we examined intestinal and brain tissues at the morphological and histological levels.RT-PCR was used to analyze the gene expression levels in different exposed groups,to analysis the effect of 3-OH-BDE47 on the TR signaling pathway from the molecular level.To prove this hypothesis,the prometamorphic tadpoles at stage 58 from a clutch were exposed to a series of concentrations of 3-OH-BDE47.We found that 3-OH-BDE47 significantly promoted T3-induced transcription of TH-response genes.Correspondingly,3-OH-BDE47 promoted T3-induced development at the morphological level.
As a new organic pollutants,OH-PBDEs is widely detected in many trophic level organisms,including human.In the rescent years,OH-PBDEs was reported to interefere with the THs fuction.In this study,we evaluated the effect of 3-OH-BDE47 on TR signaling pathway in Xenopus Laevis,what is a model organism.The tadpoles at stage 52 from a clutch were exposed to a series of concentrations of 3-OH-BDE47 in the presence of T3 for 6 days.we examined intestinal and brain tissues at the morphological and histological levels.RT-PCR was used to analyze the gene expression levels in different exposed groups,to analysis the effect of 3-OH-BDE47 on the TR signaling pathway from the molecular level.To prove this hypothesis,the prometamorphic tadpoles at stage 58 from a clutch were exposed to a series of concentrations of 3-OH-BDE47.We found that 3-OH-BDE47 significantly promoted T3-induced transcription of TH-response genes.Correspondingly,3-OH-BDE47 promoted T3-induced development at the morphological level.
引文
[1]DARNERUD P O,ERIKSEN G S,JOHANNESSON T,et al.Polybrominated diphenyl ethers:Occurrence,dietary exposure,and toxicology[J].Environmental Health Perspectives,2001,109:49-68.