丁香有效部位抑制低密度脂蛋白弱氧化修饰
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- 英文论文题名：The antioxidant inhibition effect on minimally modified low density lipoprotein of the effective fraction of clove
- 论文作者：江慎华 ; 万严 ; 杨琼玉 ; 江浩 ; 曾钧杰 ; 黄泽勇 ; 张化浩 ; 马海乐
- 英文论文作者：Jiang Shenhua ; Wan Yan ; Yang Qionyu ; Huang Zeyong ; Zhang Huahao ; Ma Haile ; School of Pharmacy and Life Science ; Jiujiang University ; School of Food and Biological Engineering ; Jiangsu University ; Jiangsu Provincal Key Lab of Physical Processing of Agricultural Products
- 年：2016
- 作者机构：九江学院药学与生命科学学院;江苏大学食品与生物工程学院江苏省农产品物理加工重点实验室;
- 论文关键词：丁香 ; 低密度脂蛋白 ; 弱氧化 ; 氧化修饰
- 英文论文关键词：clove ; low density lipoprotein ; minimally modification ; oxidative modification
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.2
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：106k
- 原文格式：O
- 会议级别：全国
摘要
低密度脂蛋白(LDL)弱氧化修饰是形成动脉粥样硬化(AS)的关键因素。实验室前期工作基础表明丁香具有很强的抗氧化活性,并确定了其抑制LDL氧化修饰的有效部位。为了研究丁香有效部位(EFC)对LDL弱氧化修饰的抑制效果,采用Fe2+体外诱导LDL弱氧化,通过测定脂质过氧化产物共轭二烯(CD)、硫代巴比妥酸反应物(TBARS)的产生、LDL紫外可见光谱及载脂蛋白B-100(apoB-100)中赖氨酸(Lys)游离氨基、色氨酸(Trp)活性的衰减程度反映抑制效果。结果表明,在LDL-弱氧化过程中,对脂质过氧化保护方面,EFC能有效延缓增殖阶段CD产生及降解阶段TBARS生成,并影响LDL紫外可见光谱。对蛋白(apoB-100)氧化保护方面,EFC(5μg/mL)能显著抑制Lys中游离氨基活性衰减(P<0.05);EFC(1μg/mL)能保护apoB-100中Trp免受破坏。结果表明EFC能有效抑制LDL弱氧化,为后续相关功能食品研发提供参考。
Minimally modified low density lipoprotein(mm-LDL) plays an important role in the development and progression of atherosclerosis.Clove has been demonstrated to possess the strong antioxidant capacity,and the effective fraction of clove(EFC) which inhibited oxidation of low density lipoprotein(LDL) has been identified in our laboratory.To further study the inhibition effect of EFC on mm-LDL,mm-LDL was induced by Fe~(2+) in vitro and inhibition effect was examined by following methods;determination of conjugated diene(CD),thiobarbituric acid reactive substances(TBARS),UV-Visible spectra,LDL-tryptophan fluorescence,free amino groups on lysine.Results indicated that the EFC may effectively delay of CD and TBARS generated during mild oxidation of LDL and change the UV-Visible spectra of LDL.Meanwhile,EFC(5μg/mL) can significantly inhibit the decay of free amino groups on lysine,and this fraction(1μg/mL) may effectively protect LDL-tryptophan from destruction(P < 0.05).Therefore,these results proved that EFC had a significandy antioxidant activity on mild oxidation of LDL,which provides reference to further research on functional food.
Minimally modified low density lipoprotein(mm-LDL) plays an important role in the development and progression of atherosclerosis.Clove has been demonstrated to possess the strong antioxidant capacity,and the effective fraction of clove(EFC) which inhibited oxidation of low density lipoprotein(LDL) has been identified in our laboratory.To further study the inhibition effect of EFC on mm-LDL,mm-LDL was induced by Fe~(2+) in vitro and inhibition effect was examined by following methods;determination of conjugated diene(CD),thiobarbituric acid reactive substances(TBARS),UV-Visible spectra,LDL-tryptophan fluorescence,free amino groups on lysine.Results indicated that the EFC may effectively delay of CD and TBARS generated during mild oxidation of LDL and change the UV-Visible spectra of LDL.Meanwhile,EFC(5μg/mL) can significantly inhibit the decay of free amino groups on lysine,and this fraction(1μg/mL) may effectively protect LDL-tryptophan from destruction(P < 0.05).Therefore,these results proved that EFC had a significandy antioxidant activity on mild oxidation of LDL,which provides reference to further research on functional food.
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