重庆巫溪烟区烟草赤星病病原鉴定
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- 英文论文题名：Identification Of Tobacco Brown Spot Pathogen In Wuxi County Of Chongqing
- 论文作者：耿莉娜 ; 帅红 ; 赵敏 ; 江厚龙 ; 李钠钾 ; 夏茜 ; 唐梅 ; 陈益银
- 英文论文作者：Geng Lina ; Shuai Hong ; Zhao Min ; Jiang Houlong ; Li Najia ; Xiaxi ; Tang Mei ; Chen Yiyin ; Chongqing Tobacco Science Research Institute ; Wuxi tobacco company of Chongqing
- 年：2016
- 作者机构：重庆烟草科学研究所;重庆市烟草公司巫溪分公司;
- 论文关键词：烟草赤星病 ; rDNA-ITS ; 细极链格孢
- 英文论文关键词：tobacco brown spot ; rDNA-ITS ; Alternaria tenuissima
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S435.72
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：7
- 文件大小：1185k
- 原文格式：D
摘要
为明确重庆市巫溪烟区烟草赤星病病原菌的种类,从重庆巫溪通城烟站不同的田块采集烟草赤星病典型病斑,分离病原菌并进行鉴定。结果显示分离到一株优势致病菌株Tb-wx-1,被证明是烟草赤星病病原菌,经对致病菌株的rDNA-ITS区序列进行测序,确定为细极链格孢Alternaria tenuissima,这是首次在重庆烟区发现细极链格孢Alternaria tenuissima引起烟草赤星病。
Typical disease spot specimens were collected from Tongcheng in Wuxi county of Chongqing and pathogen isolation and pathogenicity tests were carried out to determine tobacco brown spot pathogen species in Wuxi county of Chongqing. Results showed that the strains Tb-wx-1 isolated from disease spot was confirmed to be pathogens of tobacco brown spot. It was identified as the species of Alternaria by morphology characteristics using standard methods. The 5.8S rDNA-ITS gene sequences of the strains is identified as Alternaria tenuissima.This is the first report of Alternaria tenuissima causing tobacco brown spot.
Typical disease spot specimens were collected from Tongcheng in Wuxi county of Chongqing and pathogen isolation and pathogenicity tests were carried out to determine tobacco brown spot pathogen species in Wuxi county of Chongqing. Results showed that the strains Tb-wx-1 isolated from disease spot was confirmed to be pathogens of tobacco brown spot. It was identified as the species of Alternaria by morphology characteristics using standard methods. The 5.8S rDNA-ITS gene sequences of the strains is identified as Alternaria tenuissima.This is the first report of Alternaria tenuissima causing tobacco brown spot.
引文
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[9]祖艳青,蒋士君,王海涛等.河南省烟草赤星病病原鉴定[J].中国烟草学报,2013,04(04):73-77.
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[2]李岩,童治军,焦芳婵等.基于SSR标记对33份烟草材料的聚类分析[J].西北植物学报,2013,33(10):1972-1980.
[3]Mason E.Annotated account of fungi received at the Imperial Bureau of Mycology.ListⅡ(Fase.Ⅰ)[M].Imp Bur Mycol,Kew,Surrey.1928:43
[4]Lucas G.Alternaria alternata(Fries)Keissler,the correct name for A.tenuis and A.longipes[J].Tobacco Science,1971,15(1):37-42.
[5]张天宇,陈伟群.关于烟草赤星病病原名称的再思考[M].中国植物病理学会第六届代表大会暨学术年会.1998
[6]彭希文,刘光珍,杨永柱等.云南省烟草赤星病(Tobacco brown spot)病原研究及其防治药剂的筛选[J].西南农业大学学报:自然科学版,2004,22(2):153-101.
[7]关博元.重庆烟草赤星病菌种级地位及致病力分化研究[D];西南大学,2007.
[8]胡中会,赵立华,严恩平等.云南烟草赤星病菌及近似种rDNA-ITS序列分析[J].云南农业大学学报:自然科学版,2012,5(05):670-676.
[9]祖艳青,蒋士君,王海涛等.河南省烟草赤星病病原鉴定[J].中国烟草学报,2013,04(04):73-77.
[10]方中达.植病研究方法[M].第三版.北京:中国农业出版社,1998:122-140.
[11]陈锋菊,李百元,杨冰等.一种经济快速提取丝状真菌基因组DNA的方法[J].生命科学研究,2010,14(2):122-124.
[12]张天宇.中国真菌志(第十六卷)——链格孢属[M].第一版.北京:科学出版社,2003:1-18.