基于昆虫糖基水解酶的抑制剂筛选
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- 英文论文题名：Inhibitor screening of insect glycosyl hydrolases
- 论文作者：陈磊 ; 刘田 ; 段燕伟 ; 杨青
- 英文论文作者：Lei Chen ; Tian Liu ; Yanwei Duan ; Qing Yang ; School of Life Science and Biotechnology ; Dalian University of Technology
- 年：2016
- 作者机构：大连理工大学生命科学与技术学院;
- 论文关键词：昆虫 ; 蜕皮 ; 几丁质 ; 糖基水解酶 ; 抑制剂
- 英文论文关键词：insect ; molting ; chitin ; glycosyl hydrolases ; inhibitors
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：TQ450.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：199k
- 原文格式：D
- 会议级别：全国
摘要
几丁质作为昆虫表皮的重要组成部分,在昆虫的生长发育特别是周期性蜕皮过程中具有重要的作用。昆虫体内存在非常高效的几丁质降解系统,包含有大量的糖基水解酶负责其蜕皮过程,其中属于糖基水解酶20家族的N-乙酰己糖胺酶Hex1及18家族的几丁质酶h发挥了极为重要的作用,因此被认为是潜在的绿色农药靶标。本文以来源于亚洲玉米螟的N-乙酰己糖胺酶OfHex1及几丁质酶OfChi-h为研究对象,从微生物代谢库中筛选获得了抑制剂283和312,两者对OfHex1及OfChi-h均表现出较高的抑制活性和选择性。本研究工作为针对昆虫蜕皮过程的绿色农药开发提供了先导化合物。
Chitin,plays an important role in the molting,is an essential part of the insect cuticle.An efficient chitin degradation system which containing a large number of glycosyl hydrolases responseing molting is explored.Among all the glycosyl hydrolases,P-acetylglucosaminidases and chitinase h,which belong to family 20 and family 18 respectively,are considered to be extremely important and as a potential target for developing green pesticide.Here,an inhibitor screening of OfHex1 and OfChi-h from Ostriniafurnacalis is performed and two microbial metabolites 283 and 312 which show both high inhibitory activity and selectivity toward OfHex 1 and OfChi-h are obtained.The work here provides lead compounds for the green pesticide development.
Chitin,plays an important role in the molting,is an essential part of the insect cuticle.An efficient chitin degradation system which containing a large number of glycosyl hydrolases responseing molting is explored.Among all the glycosyl hydrolases,P-acetylglucosaminidases and chitinase h,which belong to family 20 and family 18 respectively,are considered to be extremely important and as a potential target for developing green pesticide.Here,an inhibitor screening of OfHex1 and OfChi-h from Ostriniafurnacalis is performed and two microbial metabolites 283 and 312 which show both high inhibitory activity and selectivity toward OfHex 1 and OfChi-h are obtained.The work here provides lead compounds for the green pesticide development.
引文