冰温贮藏过程中罗非鱼片蛋白质表达谱分析

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• 英文论文题名：Protein profiling analysis of skeletal muscle of tilapia,Oreochromis Niloticus, during modified atmosphere-packaged iced storage
• 论文作者：何燕富 ; 李来好 ; 黄卉 ; 杨贤庆 ; 赵永强 ; 吴燕燕
• 英文论文作者：HE Yanfu ; LI Laihao ; HUANG Hui ; YANG Xianqing ; ZHAO Yongqiang ; WU Yanyan ; South China Sea Fisheries Research Institute ; Chinese Academy of Fishery Sciences
• 年：2016
• 作者机构：农业部水产品加工重点实验室南海水产研究所中国水产科学研究院;南京农业大学无锡渔业学院学院;
• 论文关键词：罗非鱼 ; 骨骼肌 ; label free定量 ; 鲜度 ; 品质 ; 蛋白质表达谱
• 英文论文关键词：Tilapia ; skeletal muscle ; label-free quantitative ; freshness ; quality ; protein profiling
• 会议召开时间：2016-11-02
• 会议录名称：2016年中国水产学会学术年会论文摘要集
• 语种：中文
• 分类号：TS254.4
• 学会代码：OGSB
• 会议名称：2016年中国水产学会学术年会
• 会议地点：中国四川成都
• 主办单位：中国水产学会、四川省水产学会
• 学会名称：中国水产学会
• 页数：1
• 文件大小：537k
• 原文格式：D
• 会议级别：全国

摘要

本文采用了label free定量法鉴定了冰温贮藏过程中0天和7天罗非鱼片的蛋白质表达谱。一共鉴定出了902种蛋白质,其中154种为差异表达蛋白,294种缺失蛋白。对全部蛋白进行GO功能注释和KEGG代谢通路分析发现,生物过程主要为细胞过程,代谢通路则为主要的KEGG通路。差异表达蛋白的主要分子功能为结合以及催化活性功能。缺失的蛋白质主要涉及代谢过程,氧化还原过程,翻译和运输等。该研究对罗非鱼骨骼肌蛋白质组与鱼肉鲜度和品质的关系有显著的贡献,并为鱼肉鲜度变化机制及功能研究提供了新的视角。
Protein profile of the skeletal muscle of tilapia, Oreochromis Niloticus, was carried out with label-free quantitative proteomic analysis. A total of 902 tilapia muscle proteins were identified, and 154 differentially expressed proteins and 294 absent proteins from the pool of all experiments run in this study. Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG) were carried out for functional annotation and for pathway annotation. The analysis of total proteins differentially expressed proteins revealed that the top-ranking biological process was cellular process; and metabolic pathway was the top-ranking KEGG pathway. The differentially expressed proteins were focused on binding and catalytic activity in molecular function. The major biological processes of absent proteins were metabolic process, oxidation-reduction process, translation and transport. This work makes a significant contribution towards the freshness and quality of tilapia skeletal muscle proteome and provides novel insights into its role in freshness mechanism and function.

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