纤维素酶法破壁条件对雨生红球藻类胡萝卜素提取效果的影响
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- 英文论文题名：Effects of disrupting conditions on extracting carotenoid from haematococcus pluvialis by cellulase
- 论文作者：黄莹 ; 王凯 ; 董丛丛 ; 倪辉 ; 杨远帆 ; 杜希萍
- 英文论文作者：Huang ying ; Wang Kai ; Dong Congcong ; Ni Hui ; Yang Yuanfan ; Du Xiping ; College of Food and Bioengineering ; Jimei University ; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering ; Research Center of Food Biotechnology of Xiamen City ; Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed ; Xiamen Southern Ocean Technology Center of China
- 年：2016
- 作者机构：集美大学食品与生物工程学院;福建省食品微生物与酶工程重点实验室;厦门市食品与生物工程技术研究中心;厦门南方海洋研究中心海藻资源化利用与深加工重点实验室;
- 论文关键词：雨生红球藻 ; 类胡萝卜素 ; 纤维素酶 ; 提取
- 英文论文关键词：haematococcus pluvialis ; carotenoid ; cellulase ; extraction
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.2
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：112k
- 原文格式：O
- 会议级别：全国
摘要
为了评价纤维素酶法破壁在雨生红球藻类胡萝卜素提取中的应用价值,研究了酶浓度、酶反应pH、破壁温度和破壁时间4个因素对破壁后雨生红球藻类胡萝卜素提取的影响,并对破壁条件进行了优化。试验结果表明:酶浓度、酶反应pH、破壁温度和破壁时间都会对雨生红球藻破壁提取类胡萝卜素产生显著影响,但以破壁温度及酶浓度对类胡萝卜素提取量影响最大。用正交试验法得到优化的纤维素酶法破壁雨生红球藻的条件为:酶浓度0.6mg/mL、酶反应pH 4.0、破壁温度50℃、破壁时间30 min,优化后提取物中总类胡萝卜素的含量为59.62±0.31mg/g DW,比优化前提高了25%。
In order to evaluate the possibility of extracting carotenoid from Haematococcus pluvialis disrupting cell wallby cellulase,influences of several factors such as enzyme concentration,enzyme reaction pH,disrupting temperature and disrupting time on extracting carotenoid from Haematococcus pluvialis were studied and conditions used to disrupt the cell wall were optimized.Results indicated that factors such as enzyme concentration,enzyme reaction pH,disrupting temperature and disrupting time had significant effects on the extracting content of carotenoid,and disrupting temperature and enzyme concentration were the most significant for the extracting content compared with the other factors.The optimal conditions gained by orthogonal experiment were:enzyme concentration 0.6 mg/mL,enzyme reaction pH 4.0,disrupting temperature 50 ℃,disrupting time30 min.Under the optimized condition,thetotal carotenoid content in the extracting liquid was 59.62 ±0.31 mg/g DW,which increased 25%comparing with that of the non-optimized condition.
In order to evaluate the possibility of extracting carotenoid from Haematococcus pluvialis disrupting cell wallby cellulase,influences of several factors such as enzyme concentration,enzyme reaction pH,disrupting temperature and disrupting time on extracting carotenoid from Haematococcus pluvialis were studied and conditions used to disrupt the cell wall were optimized.Results indicated that factors such as enzyme concentration,enzyme reaction pH,disrupting temperature and disrupting time had significant effects on the extracting content of carotenoid,and disrupting temperature and enzyme concentration were the most significant for the extracting content compared with the other factors.The optimal conditions gained by orthogonal experiment were:enzyme concentration 0.6 mg/mL,enzyme reaction pH 4.0,disrupting temperature 50 ℃,disrupting time30 min.Under the optimized condition,thetotal carotenoid content in the extracting liquid was 59.62 ±0.31 mg/g DW,which increased 25%comparing with that of the non-optimized condition.
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