类风湿关节炎患者外周血单核细胞高表达Siglec-1及其在单个核细胞对自身抗原反应中的作用（英文）
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- 论文作者：熊怡淞 ; 程悦 ; 林秋水 ; 吴艾霖 ; 俞娟 ; 李畅 ; 孙懿 ; 仲人前 ; 吴丽娟
- 年：2013
- 作者机构：成都军区总医院临床实验医学研究与保障中心;成都军区总医院肾内科;中国人民解放军411医院骨科;第二军医大学附属长征医院实验诊断科;第二军医大学附属长海医院实验诊断科;
- 英文论文关键词：Siglec-1 ; Biological markers ; Rheumatoid arthritis ; Disease Activity Score ; Proliferation ; Activation
- 会议召开时间：2013-09-13
- 会议录名称：第四届中国临床微生物学大会暨微生物学与免疫学论坛论文汇编
- 语种：英文
- 分类号：R593.22
- 学会代码：WSWZ
- 会议名称：第四届中国临床微生物学大会暨微生物学与免疫学论坛
- 会议地点：中国江苏无锡
- 主办单位：中国微生物学会临床微生物学专业委员会、医学参考报社、宁波大学医学院附属医院
- 学会名称：中国微生物学会临床微生物学专业委员会
- 页数：1
- 文件大小：49k
- 原文格式：O
- 会议级别：全国
摘要
Objectives Elevated expression of Siglec-1 on circulating monocytes has been reported in some inflammatory and autoimmune diseases,but its expression and role in rheumatoid arthritis(RA)has not been elucidated.Methods Siglec-1protein and mRNA levels in 42 RA patients,39 osteoarthritis patients,28 SLE patients and 42 normal controls were determined by flow cytometry and QRT-PCR,respectively.In addition,ten patients with active RA were received DMARDs for 12 weeks and the frequencies of Siglec-1(+)cells and DAS28 were assessed before and after therapy.Furthermore,TNF-α,IFN-γand typeⅡcollagen were used to up-regulate Siglec-1 and PBMCs from different groups were stimulated with mitogens or antigens and cells proliferation and cytokines production were determined.Results The protein and mRNA levels of Siglec-1 on PBMCs and monocytes in RA patients were significantly higher than those in OA patients and healthy controls.Moreover,the expression of Siglec-1 protein on PBMCs was positively correlated with DAS28,ESR,hs-CRP and IgM-RF,but not with anti-CCP antibody.Interestingly,Siglec-1 expression was decreased in parallel with the decrease of DAS28 after12-week antirheumatic treatment.Furthermore,TNF-α,IFN-γand typeⅡcollagen can up-regulate Siglec-1 on PBMCs.Elevated PBMCs proliferation and pro-inflammatory cytokines production to collagen stimulation in RA patients reduced when Siglec-1 was inhibited by anti-Siglec-1 antibody.Conclusions Elevated Siglec-1 expression on PBMCs and monocytes can potentially serve as a biomarker for monitoring disease activity in RA.Siglec-1 may also play a proinflammatory role in stimulating lymphoeytes proliferation and activation in RA.
Objectives Elevated expression of Siglec-1 on circulating monocytes has been reported in some inflammatory and autoimmune diseases,but its expression and role in rheumatoid arthritis(RA) has not been elucidated.Methods Siglec-1protein and mRNA levels in 42 RA patients,39 osteoarthritis patients,28 SLE patients and 42 normal controls were determined by flow cytometry and QRT-PCR,respectively.In addition,ten patients with active RA were received DMARDs for 12 weeks and the frequencies of Siglec-1(+) cells and DAS28 were assessed before and after therapy.Furthermore,TNF-a,IFN-y and type II collagen were used to up-regulate Siglec-1 and PBMCs from different groups were stimulated with mitogens or antigens and cells proliferation and cytokines production were determined.Results The protein and mRNA levels of Siglec-1 on PBMCs and monocytes in RA patients were significantly higher than those in OA patients and healthy controls.Moreover,the expression of Siglec-1 protein on PBMCs was positively correlated with DAS28,ESR,hs-CRP and IgM-RF,but not with anti-CCP antibody.Interestingly,Siglec-1 expression was decreased in parallel with the decrease of DAS28 after12-week antirheumatic treatment.Furthermore,TNF-α,IFN-γ and type Ⅱ collagen can up-regulate Siglec-1 on PBMCs.Elevated PBMCs proliferation and pro-inflammatory cytokines production to collagen stimulation in RA patients reduced when Siglec-1 was inhibited by anti-Siglec-1 antibody.Conclusions Elevated Siglec-1 expression on PBMCs and monocytes can potentially serve as a biomarker for monitoring disease activity in RA.Siglec-1 may also play a proinflammatory role in stimulating lymphocytes proliferation and activation in RA.
Objectives Elevated expression of Siglec-1 on circulating monocytes has been reported in some inflammatory and autoimmune diseases,but its expression and role in rheumatoid arthritis(RA) has not been elucidated.Methods Siglec-1protein and mRNA levels in 42 RA patients,39 osteoarthritis patients,28 SLE patients and 42 normal controls were determined by flow cytometry and QRT-PCR,respectively.In addition,ten patients with active RA were received DMARDs for 12 weeks and the frequencies of Siglec-1(+) cells and DAS28 were assessed before and after therapy.Furthermore,TNF-a,IFN-y and type II collagen were used to up-regulate Siglec-1 and PBMCs from different groups were stimulated with mitogens or antigens and cells proliferation and cytokines production were determined.Results The protein and mRNA levels of Siglec-1 on PBMCs and monocytes in RA patients were significantly higher than those in OA patients and healthy controls.Moreover,the expression of Siglec-1 protein on PBMCs was positively correlated with DAS28,ESR,hs-CRP and IgM-RF,but not with anti-CCP antibody.Interestingly,Siglec-1 expression was decreased in parallel with the decrease of DAS28 after12-week antirheumatic treatment.Furthermore,TNF-α,IFN-γ and type Ⅱ collagen can up-regulate Siglec-1 on PBMCs.Elevated PBMCs proliferation and pro-inflammatory cytokines production to collagen stimulation in RA patients reduced when Siglec-1 was inhibited by anti-Siglec-1 antibody.Conclusions Elevated Siglec-1 expression on PBMCs and monocytes can potentially serve as a biomarker for monitoring disease activity in RA.Siglec-1 may also play a proinflammatory role in stimulating lymphocytes proliferation and activation in RA.
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