空间诱变野鸦椿组培快繁技术研究
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- 英文论文题名：Study on Tissue Propagation of Euscaphis japonica through Space Mutation
- 论文作者：陈熙 ; 马丽娟 ; 张秀英 ; 蔡邦平
- 英文论文作者：CHEN Xi ; MA Li-juan ; ZHANG Xiu-ying ; CAI Bang-ping ; College of Landscape Architecture ; Fujian Agriculture and Forestry University ; Xiamen Botanical Garden
- 年：2016
- 作者机构：福建农林大学园林学院;厦门市园林植物园;
- 论文关键词：空间诱变 ; 野鸦椿 ; 带芽茎段 ; 组织培养
- 英文论文关键词：Space mutation ; Euscaphis japonica ; Stem segment with bud ; Tissue culture
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S686
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：4
- 文件大小：509k
- 原文格式：O
- 会议级别：全国
摘要
以空间诱变野鸦椿带芽茎段为材料,进行组织培养快速繁殖研究。结果表明:最佳消毒方式为采摘后室内放置5d再进行消毒,以75%酒精消毒30s结合0.1%升汞溶液消毒8min效果最佳,污染率与死亡率均处于相对较低水平;适宜的初代培养基为MS+1.0mg·L~(-1)+NAA 0.1mg·L~(-1),萌芽率为94.44%;适宜的增殖培养基为MS+1.0mg·L~(-1)+NAA 0.1mg·L~(-1),增殖系数为4.12;适宜的生根培养基为1/3WPM+IBA0.3mg·L~(-1)+NAA0.3mg·L~(-1),生根率为69.23%。
Stem segments with buds were used as explants to study tissue culture and rapid propagation of Euscaphis japonica(Thunb.) Kanitz,which seeds had been through space mutation.The results indicated that the explants in the room for 5d after 75%alcohol disinfection 30 s and then in 0.1%HgCl_2 disinfection 8 min is suitable,the contamination rate and mortality rate are in lower level.The suitable culture medium for primary culture was MS + 1.0mg · L~(-1)+ NAA 0.1mg · L~(-1) with the rate of effective germination of 94.44%.The suitable culture medium for subculture was MS + 1.0mg · L~(-1) + NAA 0.1mg ·L~(-1) with the proliferation value of 4.12.The suitable culture medium for rooting was 1/3WPM +IBA 0.3 mg · L~(-1)+ NAA0.3mg · L~(-1),the rate of rooting was 69.23%.
Stem segments with buds were used as explants to study tissue culture and rapid propagation of Euscaphis japonica(Thunb.) Kanitz,which seeds had been through space mutation.The results indicated that the explants in the room for 5d after 75%alcohol disinfection 30 s and then in 0.1%HgCl_2 disinfection 8 min is suitable,the contamination rate and mortality rate are in lower level.The suitable culture medium for primary culture was MS + 1.0mg · L~(-1)+ NAA 0.1mg · L~(-1) with the rate of effective germination of 94.44%.The suitable culture medium for subculture was MS + 1.0mg · L~(-1) + NAA 0.1mg ·L~(-1) with the proliferation value of 4.12.The suitable culture medium for rooting was 1/3WPM +IBA 0.3 mg · L~(-1)+ NAA0.3mg · L~(-1),the rate of rooting was 69.23%.
引文
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4.杨燕凌.打破圆齿野鸦椿种子休眠及外植体选择诱导实验研究[D].福州:福建农林大学.2008.
5.涂淑萍,曹蕾,喻苏琴.圆齿野鸦椿芽继代增殖的影响因素研究[J].安徽农业科学,2009,37(28):13486-13487,13413.
6.何碧珠,何官榕,邹双全.圆齿野鸦椿叶片的植株再生及快速繁殖[J].福建农林大学学报(自然科学版),2010,39(3):257-262.
7.李玉平,邹双全,何碧珠.圆齿野鸦椿种子外植体的快繁体系[J].福建农林大学学报(自然科学版),2010,39(5):480-483.
8.刘录祥,郭会君,赵林姝,等.我国作物航天育种20年的基本成就与展望[J].核农学报,2007,21(6):589-592.
9.周秀艳,金晓霞,秦智伟,等.航天诱变育种及其在蔬菜中的应用[J].中国农学通报,2008,24(6):291-295.
2.葛玉珍.野鸦椿资源及其利用[J].中国野生植物资源,2004,23(5):24-25.
3.万志兵,刘霞,吴林金.野鸦椿组织培养的初步研究[J].湖南农业科学,2010,(13):26-27,31.
4.杨燕凌.打破圆齿野鸦椿种子休眠及外植体选择诱导实验研究[D].福州:福建农林大学.2008.
5.涂淑萍,曹蕾,喻苏琴.圆齿野鸦椿芽继代增殖的影响因素研究[J].安徽农业科学,2009,37(28):13486-13487,13413.
6.何碧珠,何官榕,邹双全.圆齿野鸦椿叶片的植株再生及快速繁殖[J].福建农林大学学报(自然科学版),2010,39(3):257-262.
7.李玉平,邹双全,何碧珠.圆齿野鸦椿种子外植体的快繁体系[J].福建农林大学学报(自然科学版),2010,39(5):480-483.
8.刘录祥,郭会君,赵林姝,等.我国作物航天育种20年的基本成就与展望[J].核农学报,2007,21(6):589-592.
9.周秀艳,金晓霞,秦智伟,等.航天诱变育种及其在蔬菜中的应用[J].中国农学通报,2008,24(6):291-295.