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不同提取方法对猴头菇粗多糖抗氧化活性的影响
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摘要
为探究不同提取方法对猴头菇粗多糖提取效果及抗氧化活性的影响。以猴头菇子实体为原料,通过热水、超声、碱液提取法分别提取猴头菇多糖,得到猴头菇粗多糖提取物:W-HeP、U-HeP和A-HeP;研究和比较了不同提取工艺对多糖得率和抗氧化活性的影响。结果表明:粗多糖得率依次为A-HeP(4.66%)>U-HeP(3.92%)>W-HeP(3.29%)。对3种粗多糖组分的还原力和DPPH·、·OH、ABTS+·清除效果分析表明3种粗多糖均具有一定的抗氧化能力,其中A-HeP对DPPH·、·OH和ABTS+·均有较好的清除效果,其清除率分别可达到72%、75%和85%。由此可见,碱液提取效果较好,碱提猴头菇粗多糖A-HeP具有较好的清除自由基能力,可作为天然抗氧化剂开发利用。
To investigate the effects of various extraction methods on the crude polysaccharides from Hericium erinaceus and their antioxidant activities,three different methods,including hot water extraction,ultrasonic extraction and alkali extraction,were applied to extract polysaccharides from the fruiting body of Hericium erinaceus and obtained three crude polysaccharides(W-HeP,U-HeP and A-HeP,respectively).The yield and antioxidant activity of the three crude polysaccharides were investigated and compared.The results showed that 4.66% of the yield was obtained for A-HeP,while only 3.92% for U-HeP and 3.29% for W-HeP.Based on the analysis of the reducing power,scavenging abilities of DPPH,hydroxyl and ABTS~+ radicals,all of the three polysaccharides showed certain antioxidant activity,especially A-Hep,which exhibited 72% of scavenging effect for DPPH·,75% for·OH and 85% for ABTS~+.These results indicated that the alkali extraction method possessed better extraction effect.Meanwhile,A-HeP had excellent scavenging radicals ability,showing the potential to be a type of natural antioxidant.
引文
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