Proteomic analysis in maize reveals the expression characteristics of proteins involved in the early grain development
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- 英文论文题名：Proteomic analysis in maize reveals the expression characteristics of proteins involved in the early grain development
- 论文作者：YU Tao ; LI Geng ; LIU Peng ; DONG Shu-ting ; ZHANG Ji-wang ; ZHAO Bin
- 英文论文作者：YU Tao ; LI Geng ; LIU Peng ; DONG Shu-ting ; ZHANG Ji-wang ; ZHAO Bin ; Agronomy College of Shandong Agricultural University/State Key Laboratory of Crop Biology
- 年：2016
- 作者机构：Agronomy College of Shandong Agricultural University/State Key Laboratory of Crop Biology;
- 英文论文关键词：Maize ; Grain developmental ; iTRAQ ; Proteomics ; Protein function
- 会议召开时间：2016-10-26
- 会议录名称：2016年全国青年作物栽培与生理学术研讨会论文集
- 语种：中文
- 分类号：S513
- 学会代码：CSSC
- 会议名称：2016年全国青年作物栽培与生理学术研讨会
- 会议地点：中国山东泰安
- 主办单位：中国作物学会
- 学会名称：中国作物学会
- 页数：16
- 文件大小：1526k
- 原文格式：O
- 会议级别：全国
摘要
The early phase of maize grain development mainly involved active cell division and enlargement,leading to increase the grain sink size for subsequent accumulation of storage material.To explore the protein synthesis,accumulation and regulation during early maize grain development,Denghai661 was used as experimental material and the middle grains were harvested after flowering artificial saturation pollination at 3,S,10 d,respectively,and then were analyzed by isobaric tags for relative and absolute quantitation(iTRAQ)proteomics.A total of 2639 proteins were identified and quantified in maize grain,and these proteins were involved in diverse biological processes and molecular functions,of which the metabolic process and molecular processes were the main biological processes,and the catalytic activity and binding function occupied the largest molecular categories,showing that these biological processes and molecular functions played important roles in maize grain development Quantitative analysis detected 137 proteins that were significantly differentially expressed "during early maize grain development,and these proteins were mainly involved in protein,stress response,cell growth and division,carbohydrate and energy,transport,secondary,starch synthesis,transcription,signal transduction and amino acid processes.Expression pattern clustering analysis showed that these different functional categories proteins synergy to express,which regulated the early maize grain development.Together,the results provide important biological basis for understanding the physiological and biochemical process and potential protein regulation during the early maize grain development.
The early phase of maize grain development mainly involved active cell division and enlargement,leading to increase the grain sink size for subsequent accumulation of storage material.To explore the protein synthesis,accumulation and regulation during early maize grain development,Denghai661 was used as experimental material and the middle grains were harvested after flowering artificial saturation pollination at 3,S,10 d,respectively,and then were analyzed by isobaric tags for relative and absolute quantitation(iTRAQ)proteomics.A total of 2639 proteins were identified and quantified in maize grain,and these proteins were involved in diverse biological processes and molecular functions,of which the metabolic process and molecular processes were the main biological processes,and the catalytic activity and binding function occupied the largest molecular categories,showing that these biological processes and molecular functions played important roles in maize grain development Quantitative analysis detected 137 proteins that were significantly differentially expressed "during early maize grain development,and these proteins were mainly involved in protein,stress response,cell growth and division,carbohydrate and energy,transport,secondary,starch synthesis,transcription,signal transduction and amino acid processes.Expression pattern clustering analysis showed that these different functional categories proteins synergy to express,which regulated the early maize grain development.Together,the results provide important biological basis for understanding the physiological and biochemical process and potential protein regulation during the early maize grain development.
The early phase of maize grain development mainly involved active cell division and enlargement,leading to increase the grain sink size for subsequent accumulation of storage material.To explore the protein synthesis,accumulation and regulation during early maize grain development,Denghai661 was used as experimental material and the middle grains were harvested after flowering artificial saturation pollination at 3,S,10 d,respectively,and then were analyzed by isobaric tags for relative and absolute quantitation(iTRAQ)proteomics.A total of 2639 proteins were identified and quantified in maize grain,and these proteins were involved in diverse biological processes and molecular functions,of which the metabolic process and molecular processes were the main biological processes,and the catalytic activity and binding function occupied the largest molecular categories,showing that these biological processes and molecular functions played important roles in maize grain development Quantitative analysis detected 137 proteins that were significantly differentially expressed "during early maize grain development,and these proteins were mainly involved in protein,stress response,cell growth and division,carbohydrate and energy,transport,secondary,starch synthesis,transcription,signal transduction and amino acid processes.Expression pattern clustering analysis showed that these different functional categories proteins synergy to express,which regulated the early maize grain development.Together,the results provide important biological basis for understanding the physiological and biochemical process and potential protein regulation during the early maize grain development.
引文
[1]Sabelli P A,Larkins B A.The development of endosperm in grasses.Plant Physiol,2009,149(1):14-26
[2]旷仁平,姜孝成,刘姜瑾,张春来,Adrian S.胚乳的发育及其调控.植物生理学报,2006,42(2):182-190Kuang R P,Jiang X C,Liu J J,Zhang C L,Adrian S.Endosperm development and its regulation.J Plant Physiol,2006,42(2):182-190(in Chinese with English abstract)
[3]左振朋,王婧,董鲁浩,马登超,孙庆泉,董树亭.不同品质类型玉米籽粒充实期的胚乳细胞增殖与生理活性比较.作物学报,2010,36(5):848-855Zuo Z P,Wang J,Dong L H,Ma D C,Sun Q Q,Dong S T.Comparison of multiplication of endosperm cell and physiological activity in developing kernels among normal corn,glutinous corn,and pop corn.Acta Agron Sin,2010,36(5):848-855(in Chinese with English abstract)
[4]Li G S,Wang D F,Yang R L,Logan K,Chen H,Zhang S S,Skaggs M I,Lloyd A,Burnett W J,Laurie J D,Hunter B G,Dannenhoffer J M,Larkins B A,Drews G N,Wang X F,Yadegari R.Temporal patterns of gene expression in developing maize endosperm identified through transcriptome sequencing.PNAS,2014,111(21):7582-7587
[5]Chen J,Zeng B,Zhang M,Xie S,Wang G,Hauck A.Dynamic transcriptome landscape of maize embryo and endosperm development.Plant Physiol,2014,166(1):252-264
[6]Hajduch M,Hearne L B,Miemyk J A,Casteel J E,Joshi T,Agrawal G K.Systems analysis of seed filling in Arabidopsis:using general linear modeling to assess concordance of transcript and protein expression.Plant Physiol,2010,152(4):2078-2087
[7]Nadaud I,Girousse C,Debiton C,Chambon C,Bouzidi M F,Martre P.Proteomic and morphological analysis of early stages of wheat grain development.Proteomics,2010,10(16):2901-2910
[8]Mechin V,Balliau T,Chateau-Joubert S,Davanture M,Langella O,Negroni L.A two-dimensional proteome map of maize endosperm.Phytochemistry,2004,65(11):1609-1618
[9]Jin X,Fu Z,Ding D,Li W,Liu Z,Tang J.Proteomic identification of genes associated with maize grain-filling rate.Plos One,2013,8(3):e59353
[10]Huang H,Mller I M,Song S Q.Proteomics of desiccation tolerance during development and germination of maize embryos.J Proteomics,2011,75(4):1247-1262
[11]吴林坤,陈军,吴红淼,王娟英,秦贤金,张重义,林文雄.地黄连作胁迫响应机制的块根蛋白质组学分析.作物学报,2016,42(2):243-254Wu L K,Chen J,Wu H M,Wang J Y,Qin X J,Zhang Z Y,Lin W X.Comparative Proteomics analysis of R.glutinosa tuber root in response to consecutive monoculture.Acta Agron Sin,2016,42(2):243-254(in Chinese with English abstract)
[12]韩平安,逯晓萍,米福贵,张瑞霞,李美娜,薛春雷,董婧,丛梦露.基于蛋白质组学的高丹草苗期杂种优势分析.作物学报,2016,42(5):696-705Han P A,Lu X P,Mi F G,Zhang R X,Li M N,Xue C L,Dong J,Cong M L.Analysis of heterosis in sorghum-sudangrass hybrid seedlings based on proteomics.Acta Agron Sin,2016,42(5):696-705(in Chinese with English abstract)
[13]于涛,李耕,刘鹏,董树亭,张吉旺,赵斌,柏晗.玉米早期发育阶段粒位效应的蛋白质组学分析.中国农业科学,2016,49(1):54-68Yu T,Li Q Liu P,Dong S T,Zhang J W,Zhao B,Bai H.Proteomics analysis of grain position effects during early developmental stages of maize.Sci Agric Sin,2016,49(1):54-68(in Chinese with English abstract)
[14]Lilley K S,Razzaq A,Dupree P.Two-dimensional gel electrophoresis:recent advances in sample preparation,detection and quantitation.Curr Opin Chem Biol,2002,6(1):46-50
[15]Ma C,Zhou J,Chen G,Bian Y,Lv D,Li X Wang Z,Yan Y.iTRAQ-based quantitative proteome and phosphoprotein characterization reveals the central metabolism changes involved in wheat grain development BMC Genomics,2014,15(1):1-20
[16]Wasteneys G O,Yang Z.New views on the plant cytoskeleton.Plant Physiol,2004,136(4):3884-3891
[17]Miernyk J A.Seed proteomics.J Proteomics,2011,74(4):389-400
[18]Schippers J H M,Mueller-Roeber B.Ribosomal composition and control of leaf development.Plant Sci,2010,179(4):307-315
[19]Lee J,Koh H J.A label-free quantitative shotgun proteomics analysis of rice grain development.Proteome Sci,2011,9(1):1-10
[20]Zhang Z Y,Li J H,Liu H H,Chong K,Xu Y Y.Roles of ubiquitination-mediated protein degradation in plant responses to abiotic stresses.Environ Exp Bot,2015,114:92-103
[21]Gutierrez-Marcos J F,Costa L M,Evans M M S.Maternal gametophytic baseless 1 is required for development of the central cell and early endosperm patterning in maize(Zea mays).Genetics,2006,174(1):317-329
[22]Gutierrez L,Van W O,Castelain M,Bellini C.Combined networks regulating seed maturation.Trends Plant Sci,2007,12(7):294-300
[23]Kroger N J,Schaewen A V.The oxidative pentose phosphate pathway:structure and organisation.Curr Opin Plant Biol,2003,6(3):236-246
[24]张海艳.玉米胚乳细胞淀粉质体的发育和增殖方式.玉米科学,2009,17(4):58-60Zhang H Y.Amyloplast development and proliferation in maize starch endosperm cell.J Maize Sci,2009,17(4):58-60(in Chinese with English abstract)
[25]RoUetschek H,Koch K,Wobus U,Borisjuk L.Positional cues for thestarch/lipid balance in maize kernels and resource partitioning to the embryo.Plant J,2005,42(1):69-83
[26]Mittler R,Vanderauwera S,Gollery M,Breusegem F V.Reactive oxygen gene network of plants.Trends Plant Sci,2004,9(10):490-498
[27]Kolomiets M V,Hannapel D J,Chen H,Tymeson M,Gladon R J.Lipoxygenase is involved in the control of potato tuber development.Plant Cell,2001,13(3):613-626
[28]宫长荣,李艳梅,杨立均.水分胁迫下离体烟叶中腊氧合酶活性、水杨酸与荣莉酸积累的关系.中国农业科学,2003,36(3):269-272Gong C R,Li Y M,Yang L J.Relationship between LOX activity and SA and JA accumulations in tobacco leave under water stress.Sci Agric Sin,2003,36(3):269-272(in Chinese with English abstract)
[29]Fontecave M,Atta M,Mulliez E.S-adenosylmethionine:nothing goes to waste.Trends Biochem Sci,2004,29(5):243-249
[30]Feng H Y,Wang Z.M,Kong F N,Zhang M J,Zhou S L.Roles of carbohydrate supply and ethylene,polyamines in maize kernel set.J Integr Plant Biol,2011,53(5):388-398
[2]旷仁平,姜孝成,刘姜瑾,张春来,Adrian S.胚乳的发育及其调控.植物生理学报,2006,42(2):182-190Kuang R P,Jiang X C,Liu J J,Zhang C L,Adrian S.Endosperm development and its regulation.J Plant Physiol,2006,42(2):182-190(in Chinese with English abstract)
[3]左振朋,王婧,董鲁浩,马登超,孙庆泉,董树亭.不同品质类型玉米籽粒充实期的胚乳细胞增殖与生理活性比较.作物学报,2010,36(5):848-855Zuo Z P,Wang J,Dong L H,Ma D C,Sun Q Q,Dong S T.Comparison of multiplication of endosperm cell and physiological activity in developing kernels among normal corn,glutinous corn,and pop corn.Acta Agron Sin,2010,36(5):848-855(in Chinese with English abstract)
[4]Li G S,Wang D F,Yang R L,Logan K,Chen H,Zhang S S,Skaggs M I,Lloyd A,Burnett W J,Laurie J D,Hunter B G,Dannenhoffer J M,Larkins B A,Drews G N,Wang X F,Yadegari R.Temporal patterns of gene expression in developing maize endosperm identified through transcriptome sequencing.PNAS,2014,111(21):7582-7587
[5]Chen J,Zeng B,Zhang M,Xie S,Wang G,Hauck A.Dynamic transcriptome landscape of maize embryo and endosperm development.Plant Physiol,2014,166(1):252-264
[6]Hajduch M,Hearne L B,Miemyk J A,Casteel J E,Joshi T,Agrawal G K.Systems analysis of seed filling in Arabidopsis:using general linear modeling to assess concordance of transcript and protein expression.Plant Physiol,2010,152(4):2078-2087
[7]Nadaud I,Girousse C,Debiton C,Chambon C,Bouzidi M F,Martre P.Proteomic and morphological analysis of early stages of wheat grain development.Proteomics,2010,10(16):2901-2910
[8]Mechin V,Balliau T,Chateau-Joubert S,Davanture M,Langella O,Negroni L.A two-dimensional proteome map of maize endosperm.Phytochemistry,2004,65(11):1609-1618
[9]Jin X,Fu Z,Ding D,Li W,Liu Z,Tang J.Proteomic identification of genes associated with maize grain-filling rate.Plos One,2013,8(3):e59353
[10]Huang H,Mller I M,Song S Q.Proteomics of desiccation tolerance during development and germination of maize embryos.J Proteomics,2011,75(4):1247-1262
[11]吴林坤,陈军,吴红淼,王娟英,秦贤金,张重义,林文雄.地黄连作胁迫响应机制的块根蛋白质组学分析.作物学报,2016,42(2):243-254Wu L K,Chen J,Wu H M,Wang J Y,Qin X J,Zhang Z Y,Lin W X.Comparative Proteomics analysis of R.glutinosa tuber root in response to consecutive monoculture.Acta Agron Sin,2016,42(2):243-254(in Chinese with English abstract)
[12]韩平安,逯晓萍,米福贵,张瑞霞,李美娜,薛春雷,董婧,丛梦露.基于蛋白质组学的高丹草苗期杂种优势分析.作物学报,2016,42(5):696-705Han P A,Lu X P,Mi F G,Zhang R X,Li M N,Xue C L,Dong J,Cong M L.Analysis of heterosis in sorghum-sudangrass hybrid seedlings based on proteomics.Acta Agron Sin,2016,42(5):696-705(in Chinese with English abstract)
[13]于涛,李耕,刘鹏,董树亭,张吉旺,赵斌,柏晗.玉米早期发育阶段粒位效应的蛋白质组学分析.中国农业科学,2016,49(1):54-68Yu T,Li Q Liu P,Dong S T,Zhang J W,Zhao B,Bai H.Proteomics analysis of grain position effects during early developmental stages of maize.Sci Agric Sin,2016,49(1):54-68(in Chinese with English abstract)
[14]Lilley K S,Razzaq A,Dupree P.Two-dimensional gel electrophoresis:recent advances in sample preparation,detection and quantitation.Curr Opin Chem Biol,2002,6(1):46-50
[15]Ma C,Zhou J,Chen G,Bian Y,Lv D,Li X Wang Z,Yan Y.iTRAQ-based quantitative proteome and phosphoprotein characterization reveals the central metabolism changes involved in wheat grain development BMC Genomics,2014,15(1):1-20
[16]Wasteneys G O,Yang Z.New views on the plant cytoskeleton.Plant Physiol,2004,136(4):3884-3891
[17]Miernyk J A.Seed proteomics.J Proteomics,2011,74(4):389-400
[18]Schippers J H M,Mueller-Roeber B.Ribosomal composition and control of leaf development.Plant Sci,2010,179(4):307-315
[19]Lee J,Koh H J.A label-free quantitative shotgun proteomics analysis of rice grain development.Proteome Sci,2011,9(1):1-10
[20]Zhang Z Y,Li J H,Liu H H,Chong K,Xu Y Y.Roles of ubiquitination-mediated protein degradation in plant responses to abiotic stresses.Environ Exp Bot,2015,114:92-103
[21]Gutierrez-Marcos J F,Costa L M,Evans M M S.Maternal gametophytic baseless 1 is required for development of the central cell and early endosperm patterning in maize(Zea mays).Genetics,2006,174(1):317-329
[22]Gutierrez L,Van W O,Castelain M,Bellini C.Combined networks regulating seed maturation.Trends Plant Sci,2007,12(7):294-300
[23]Kroger N J,Schaewen A V.The oxidative pentose phosphate pathway:structure and organisation.Curr Opin Plant Biol,2003,6(3):236-246
[24]张海艳.玉米胚乳细胞淀粉质体的发育和增殖方式.玉米科学,2009,17(4):58-60Zhang H Y.Amyloplast development and proliferation in maize starch endosperm cell.J Maize Sci,2009,17(4):58-60(in Chinese with English abstract)
[25]RoUetschek H,Koch K,Wobus U,Borisjuk L.Positional cues for thestarch/lipid balance in maize kernels and resource partitioning to the embryo.Plant J,2005,42(1):69-83
[26]Mittler R,Vanderauwera S,Gollery M,Breusegem F V.Reactive oxygen gene network of plants.Trends Plant Sci,2004,9(10):490-498
[27]Kolomiets M V,Hannapel D J,Chen H,Tymeson M,Gladon R J.Lipoxygenase is involved in the control of potato tuber development.Plant Cell,2001,13(3):613-626
[28]宫长荣,李艳梅,杨立均.水分胁迫下离体烟叶中腊氧合酶活性、水杨酸与荣莉酸积累的关系.中国农业科学,2003,36(3):269-272Gong C R,Li Y M,Yang L J.Relationship between LOX activity and SA and JA accumulations in tobacco leave under water stress.Sci Agric Sin,2003,36(3):269-272(in Chinese with English abstract)
[29]Fontecave M,Atta M,Mulliez E.S-adenosylmethionine:nothing goes to waste.Trends Biochem Sci,2004,29(5):243-249
[30]Feng H Y,Wang Z.M,Kong F N,Zhang M J,Zhou S L.Roles of carbohydrate supply and ethylene,polyamines in maize kernel set.J Integr Plant Biol,2011,53(5):388-398