经膜分离的丝胶肽的理化性质、冷冻保护活性和作用机制研究

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• 英文论文题名：Investigation of physiochemicalproperties,cryoprotective activity and possible mechanism of sericin peptides derived from membrane separation
• 论文作者：李灵 ; 汪少芸
• 英文论文作者：Li Ling ; Wang Shaoyun ; College of Biological Science and Engineering ; Fuzhou University
• 年：2016
• 作者机构：福州大学生物科学与工程学院;
• 论文关键词：丝胶肽 ; 理化性质 ; 冷冻保护活性 ; 保加利亚德氏乳酸杆菌 ; 机制
• 英文论文关键词：sericin peptides ; physiochemical property ; cryoprotective activity ; lactobacillus delbrueckii subsp.bulgaricus mechanism
• 会议召开时间：2016-11-09
• 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
• 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
• 语种：中文
• 分类号：TS201.2
• 学会代码：ZGSP
• 会议名称：中国食品科学技术学会第十三届年会
• 会议地点：中国北京
• 主办单位：中国食品科学技术学会
• 学会名称：中国食品科学技术学会
• 页数：2
• 文件大小：110k
• 原文格式：O
• 会议级别：全国

摘要

为了开发3K-SP工业应用,对经3k膜分离的丝胶肽(3K-SP)的理化性质和冷冻保护功能进行了研究。结果表明,72%的3K-SP分子量分布于180~1000Da之间,富含丝氨酸(26.14%),天门冬氨酸(15.83%),甘氨酸(8.88%),苏氨酸(7.51%),谷氨酸(6.89%),冰结构蛋白的抗冻活性与这些特定氨基酸有关。与对照组相比,添加有3K-SP的冷冻液能在较低的温度和较短的时间内融化。此外,3K-SP能够在冷冻液中保持小冰晶的尺寸,具有冰晶重结晶抑制活性。此外,在冷冻过程中,3K-SP对保加利亚德氏乳酸杆菌表现出高的低温保护活性。3K-SP保护细胞的最佳条件为:质量浓度1mg/mL,pH 7.0,细胞在此条件下的存活率高达84.78±3.07%。流式细胞仪和扫描电子显微镜分析发现,添加有3K-SP实验组的细胞的存活率从52.9%增加到80.1%,这表明3K-SP可能通过与细胞膜问的相互作用来介导它的保护效果.由此使细胞包围在玻璃状基质中.维持细胞膜完整性。
In order to exploit their industrial applications,sericin peptides(3K-SP) were obtained by membrane separation and their physiochemical properties and cryoprotective function were investigated.Results showed that the molecular mass of most(72%) 3K-SP were between 180 and 1000 Da,and 3K-SP was rich in Ser(26.14%),Asp(15.83%),Gly(8.88%),Thr(7.51%),and Glu(6.89%),which have been associated with the cryoprotective activity of ice-structuring proteins.Addition of 3K-SP to a frozen solution led to a reduction in melting temperature and a reduction in melting time compared to control solution.In addition,3K-SP could inhibited ice recrystallization,since it was able to maintain small ice crystal sizes within a frozen solution.Furthermore,3K-SP demonstrated excellent cryogenic protection activity on Lactobacillus delbrueckii Subsp.Bulgaricus during freezing.3K-SP provided optimal protection of cells at conditions in which its concentration was 1.0 mg/mL and the pH of the solution was 7.0.In these conditions,the percentage of surviving cells was as high as84.78 ±3.07%.Flow cytometric and scanning electron microscopy analyses showed that treatment with 3K-SP increased the percentage of viable cells from 52.9%to 80.1%,suggesting that 3K-SP may mediate its protective effects through interaction with cell membranes,whereby it surrounds cells in a glassy matrix that helps maintain their membrane integrity.

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