氢氧化钠/尿素法和硫酸法测定烟草木质素含量的对比分析
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- 英文论文题名：Comparison of Na OH/urea Method with Sulfuric Acid Method for Determining the Lignin Content in Tobacco
- 论文作者：孔浩辉 ; 李秀丽 ; 黄翼飞 ; 纪楷滨 ; 楼宏铭 ; 陈森林 ; 邱学青 ; 杨东杰
- 英文论文作者：Kong Haohui ; Li Xiuli ; Huang Yifei ; Ji Kaibin ; Lou Hongming ; Chen Senlin ; Qiu Xueqing ; Yang Dongjie ; Technology Center ; China Tobacco Guangdong Industrial Co. ; Ltd. ; School of Chemistry and Chemical Engineering ; South China University of Technology ; Guangzhou Quality Supervision and Inspection Research Institute ; State Key Laboratory of Pulp and Paper Engineering ; South China University of Technology
- 年：2016
- 作者机构：广东中烟工业有限责任公司技术中心;华南理工大学化学与化工学院;广州市质量监督检测研究院;华南理工大学制浆造纸工程国家重点实验室;
- 论文关键词：烟草 ; 木质素 ; 含量 ; 硫酸 ; 氢氧化钠/尿素
- 英文论文关键词：Tobacco ; Lignin ; Content ; Sulfuric acid ; Sodium hydrate/urea
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草工业主题
- 语种：中文
- 分类号：TS42
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：11
- 文件大小：1850k
- 原文格式：D
摘要
为了对比氢氧化钠/尿素法(NU法)和硫酸法在测定烟草中木质素含量的差异性,采用NU法和硫酸法测定广东、四川、湖南、云南和贵州5个产区烟梗和烟叶的木质素含量,分析了两种方法在预处理过程的干扰物质去除率和酸解过程木质素含量和结构变化。结果表明:①与硫酸法相比,NU法预处理对干扰物质的去除率更高。②NU法采用稀酸酸解,减少了烟草中木质素的降解;选择在325 nm处测定酸溶木质素(ASL)的紫外吸光度,避免了蛋白质和糠醛对ASL的干扰,提高了烟草木质素测定结果的准确度和重现性。③与硫酸法相比,NU法分离得到的酸不溶木质素(AIL)的氧化程度低、结构变化小,因而更能反映烟草木质素的结构特征。NU法比硫酸法更加适用于烟草木质素的含量测定。
In order to compared the difference effect on determining lignin content in the tobacco. The removal rate, lignin content and structural changes were analyzed in pretreatment and acid treatment process. Determining the lignin content in tobacco by Na OH/Urea method and sulfuric acid method, which is produced from province of Guangdong, Sichuan, Yunnan, Guizhou and Hunan, respectively. The study results showed that the removal rate in pretreatmentof Na OH/urea method is higher than that of sulfuric acid method.Na OH/urea method selected UV absorbance in 325 nm to quantify the content of acid-soluble lignin, which will avoid the interference of protein and furfural. By treating it in dilute sulfuric acid solution can reduce the lignin degradation and structural change of tobacco lignin.The lignin content determination by Na OH/urea method has good reproducibility and accuracy. The lignin isolated by Na OH/urea method had lower degree of oxidation and smaller structural change than that of sulfuric acid method. Na OH/urea method is more suitable for tobacco lignin content determination than sulfuric acid method.
In order to compared the difference effect on determining lignin content in the tobacco. The removal rate, lignin content and structural changes were analyzed in pretreatment and acid treatment process. Determining the lignin content in tobacco by Na OH/Urea method and sulfuric acid method, which is produced from province of Guangdong, Sichuan, Yunnan, Guizhou and Hunan, respectively. The study results showed that the removal rate in pretreatmentof Na OH/urea method is higher than that of sulfuric acid method.Na OH/urea method selected UV absorbance in 325 nm to quantify the content of acid-soluble lignin, which will avoid the interference of protein and furfural. By treating it in dilute sulfuric acid solution can reduce the lignin degradation and structural change of tobacco lignin.The lignin content determination by Na OH/urea method has good reproducibility and accuracy. The lignin isolated by Na OH/urea method had lower degree of oxidation and smaller structural change than that of sulfuric acid method. Na OH/urea method is more suitable for tobacco lignin content determination than sulfuric acid method.
引文
[1]武圣江,李先锋,王国良,等.烟叶细胞壁和细胞超微结构研究进展[J].浙江农业科学,2010(1):15-18.
[2]Freudenberg K,Neish A C.Constitution and biosynthesis of lignin[M].Berlin:Springer-Verlag,1968.
[3]Davis D L,Nielsen M T.烟草——生产,化学和技术[M].国家烟草专卖局科技教育司,中国烟草科技信息中心,译.北京:化学工业出版社,2002,255-257.
[4]闰克玉.烟草化学[M].郑州:郑州大学出版社,2002:43-56.
[5]朱大恒,李彩霞,张爱忠,等.烟气有害成分与烟叶化学成分的关系[J].烟草科技,1999(4):25-26.
[6]谭洪,王树荣,骆仲泱,等.木质素快速热裂解试验研究[J].浙江大学学报:工学版,2005,39(5):710-714.
[7]Wu S,Argyropoulos D S.An improved method for isolating lignin in high yield and purity[J].Pulp and Paper Science,2003,29(7):235-240.
[8]Johnson D B,Moore W E,Zank L C.The spectro photometric detemination of lignin insmall wood samples[J].Tappi,1961,44(11):789-793.
[9]童国林,横山朝哉,松本雄二,等.氧漂过程中脱木质素与木质素氧化之间定量关系的研究[J].林产化学与工业,2005,25(4):51-55.
[10]GB/T10337—2008造纸原料和纸浆中酸溶木素的测定[S].
[11]GB/T 747—2003纸浆酸不溶木素的测定[S].
[12]TAPPI T 222 om-02 Acid-insoluble lignin in wood and pulp[S].TAPPI Test Methods,Atlanta,GA,USA:Tappi Press,2002.
[13]TAPPIUM 250Acid soluble lignin in wood and pulp[S].TAPPI Useful Methods,Atlanta,GA,USA:Tappi Press,1991.
[14]邓宇.烟叶中木质素的测定、裂解和降解研究[D].江苏:江南大学食品工程,2006.
[15]杨斌,殷引,张浩博,等.洗涤剂法测定烟草及烟草制品中中性洗涤纤维、酸性洗涤纤维、酸性洗涤木质素的研究[J].中国烟草学报,2012,18(3):10-15.
[16]韩富根.烟草化学[M].北京:中国农业出版社,2010:9.
[17]杨淑蕙.植物纤维化学[M].3版.北京:中国轻工业出版社,2001:6-14.
[18]Cai J,Zhang L N,Liu S L,et al.Dynamic self-assembly induced rapid dissolution of cellulose at low temperatures[J].Macromolecules,2008,41(23):9345-9351.
[19]Rusa C C,Tonelli A E.Separation of polymers by molecular weight through inclusion compound formation with urea andα-cyclodextrinhosts[J].Macromolecules,2000,33(5):1813-1818.
[20]Mascal M,Infantes L,Chisholm J.Water oligomers in crystal hydrates—what's news and what isn't[J].Angew Chem Int Ed,2006,45(1):32-36.
[21]Lee S O,Kariuki B M,Harris K D M.Design of a Bilayer Structure in an Organic Inclusion Compound[J].Angew Chem Int Ed,2002,41(12):2181-2184.
[22]Yuan T Q,Sun R C,He J,et al.Fractionation and physico-chemical analysis of degraded lignins from the black liquor of Eucalyptus pellita KP-AQ pulping[J].Polymer Degradation and Stability,2009,4(7):1146-1147.
[23]Tejado A,Pefia C,Labidi J,et al.Physico-chemical characterization of lignin from different sources for use in phenol-formaldehyde resin synthesis[J].Bioresource Technology,2007,98(8):1657-1659.
[24]孔浩辉,李秀丽,黄翼飞,等.Na OH/尿素低温溶解法测定烟梗木质素含量的研究[J].中国烟草学报,2014,20(3):9-15.
[25]孙素琴,周群,陈建波.中药红外光谱分析与鉴定[M].1版.北京:中国轻工业出版社,2010:68.
[26]Nlewem K C,Thrash Jr M E.Comparison of different pretreatment methods based on residual lignin effect on the enzymatic hydrolysis of switchgrass[J].Bioresource technology,2010,101(14):5426-5430.
[27]Mussatto S I,Fernandes M,Milagres AM F,et al.Effect of hemicellulose and lignin on enzymatic hydrolysis of cellulose from brewer's spent grain[J].Enzyme and Microbial Technology,2008,43(2):124-129.
[28]Faix O.Classification of lignins from different botanical origins by FT-IR spectroscopy[J].Holzforschung,1991,45(S1):21-28.
[29]Ucar G,Meier D,Faix O,et al.Analytical pyrolysis and FTIR spectroscopy of fossil Sequoiadendron giganteum(Lindl.)wood and MWLs isolated hereof[J].Holzals Rohund Werkstoff,2005,63(1):57-63.
[30]Abdulkhani A,Karimi A,Mirshokraie A,et al.Isolation and chemical structure characterization of enzymatic lignin from Populus deltoids wood[J].Applied Polymer Science,2013,130(1):710.
[31]Scopes R K.Measurement of protein by spectrophotometry at 205 nm[J].Analytical biochemistry,1974,59(1):252-277.
[32]Yasuda S,Fukushima K,Kakehi A.Formation and chemical structures of acid-soluble lignin I:Sulfuric acid treatment time and acid-soluble lignin content of hardwood[J].Japan Wood Research Society,2001,47:69-72.
[33]Kung S D,Saunder J A,Tso T C,et al.Tobacco as a potential food source and smoke material:Nutritional evaluation of tobacco leaf protein[J].Journal of food science,1980,45(2):320-322,327.
[34]戴明,李旭东,魏雅冬,等.烟梗组织结构与吸水性关系的研究[J].安徽农业科学,2011,39(4):2309-2311.
[2]Freudenberg K,Neish A C.Constitution and biosynthesis of lignin[M].Berlin:Springer-Verlag,1968.
[3]Davis D L,Nielsen M T.烟草——生产,化学和技术[M].国家烟草专卖局科技教育司,中国烟草科技信息中心,译.北京:化学工业出版社,2002,255-257.
[4]闰克玉.烟草化学[M].郑州:郑州大学出版社,2002:43-56.
[5]朱大恒,李彩霞,张爱忠,等.烟气有害成分与烟叶化学成分的关系[J].烟草科技,1999(4):25-26.
[6]谭洪,王树荣,骆仲泱,等.木质素快速热裂解试验研究[J].浙江大学学报:工学版,2005,39(5):710-714.
[7]Wu S,Argyropoulos D S.An improved method for isolating lignin in high yield and purity[J].Pulp and Paper Science,2003,29(7):235-240.
[8]Johnson D B,Moore W E,Zank L C.The spectro photometric detemination of lignin insmall wood samples[J].Tappi,1961,44(11):789-793.
[9]童国林,横山朝哉,松本雄二,等.氧漂过程中脱木质素与木质素氧化之间定量关系的研究[J].林产化学与工业,2005,25(4):51-55.
[10]GB/T10337—2008造纸原料和纸浆中酸溶木素的测定[S].
[11]GB/T 747—2003纸浆酸不溶木素的测定[S].
[12]TAPPI T 222 om-02 Acid-insoluble lignin in wood and pulp[S].TAPPI Test Methods,Atlanta,GA,USA:Tappi Press,2002.
[13]TAPPIUM 250Acid soluble lignin in wood and pulp[S].TAPPI Useful Methods,Atlanta,GA,USA:Tappi Press,1991.
[14]邓宇.烟叶中木质素的测定、裂解和降解研究[D].江苏:江南大学食品工程,2006.
[15]杨斌,殷引,张浩博,等.洗涤剂法测定烟草及烟草制品中中性洗涤纤维、酸性洗涤纤维、酸性洗涤木质素的研究[J].中国烟草学报,2012,18(3):10-15.
[16]韩富根.烟草化学[M].北京:中国农业出版社,2010:9.
[17]杨淑蕙.植物纤维化学[M].3版.北京:中国轻工业出版社,2001:6-14.
[18]Cai J,Zhang L N,Liu S L,et al.Dynamic self-assembly induced rapid dissolution of cellulose at low temperatures[J].Macromolecules,2008,41(23):9345-9351.
[19]Rusa C C,Tonelli A E.Separation of polymers by molecular weight through inclusion compound formation with urea andα-cyclodextrinhosts[J].Macromolecules,2000,33(5):1813-1818.
[20]Mascal M,Infantes L,Chisholm J.Water oligomers in crystal hydrates—what's news and what isn't[J].Angew Chem Int Ed,2006,45(1):32-36.
[21]Lee S O,Kariuki B M,Harris K D M.Design of a Bilayer Structure in an Organic Inclusion Compound[J].Angew Chem Int Ed,2002,41(12):2181-2184.
[22]Yuan T Q,Sun R C,He J,et al.Fractionation and physico-chemical analysis of degraded lignins from the black liquor of Eucalyptus pellita KP-AQ pulping[J].Polymer Degradation and Stability,2009,4(7):1146-1147.
[23]Tejado A,Pefia C,Labidi J,et al.Physico-chemical characterization of lignin from different sources for use in phenol-formaldehyde resin synthesis[J].Bioresource Technology,2007,98(8):1657-1659.
[24]孔浩辉,李秀丽,黄翼飞,等.Na OH/尿素低温溶解法测定烟梗木质素含量的研究[J].中国烟草学报,2014,20(3):9-15.
[25]孙素琴,周群,陈建波.中药红外光谱分析与鉴定[M].1版.北京:中国轻工业出版社,2010:68.
[26]Nlewem K C,Thrash Jr M E.Comparison of different pretreatment methods based on residual lignin effect on the enzymatic hydrolysis of switchgrass[J].Bioresource technology,2010,101(14):5426-5430.
[27]Mussatto S I,Fernandes M,Milagres AM F,et al.Effect of hemicellulose and lignin on enzymatic hydrolysis of cellulose from brewer's spent grain[J].Enzyme and Microbial Technology,2008,43(2):124-129.
[28]Faix O.Classification of lignins from different botanical origins by FT-IR spectroscopy[J].Holzforschung,1991,45(S1):21-28.
[29]Ucar G,Meier D,Faix O,et al.Analytical pyrolysis and FTIR spectroscopy of fossil Sequoiadendron giganteum(Lindl.)wood and MWLs isolated hereof[J].Holzals Rohund Werkstoff,2005,63(1):57-63.
[30]Abdulkhani A,Karimi A,Mirshokraie A,et al.Isolation and chemical structure characterization of enzymatic lignin from Populus deltoids wood[J].Applied Polymer Science,2013,130(1):710.
[31]Scopes R K.Measurement of protein by spectrophotometry at 205 nm[J].Analytical biochemistry,1974,59(1):252-277.
[32]Yasuda S,Fukushima K,Kakehi A.Formation and chemical structures of acid-soluble lignin I:Sulfuric acid treatment time and acid-soluble lignin content of hardwood[J].Japan Wood Research Society,2001,47:69-72.
[33]Kung S D,Saunder J A,Tso T C,et al.Tobacco as a potential food source and smoke material:Nutritional evaluation of tobacco leaf protein[J].Journal of food science,1980,45(2):320-322,327.
[34]戴明,李旭东,魏雅冬,等.烟梗组织结构与吸水性关系的研究[J].安徽农业科学,2011,39(4):2309-2311.