黄芪多糖对H5N1禽流感疫苗的免疫增强效应研究
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- 英文论文题名：The Study of immunity enhancing effect of APS on H5N1 avian influenza vaccine
- 论文作者：许国洋 ; 付利芝 ; 张素辉 ; 徐登峰 ; 戴茜茜 ; 杨金龙
- 英文论文作者：XU Guo-yang ; Fu Li-zhi ; ZHANG Su-hui ; XU Deng-feng ; DAI Xi-xi ; YANG Jin-long ; Chongqing academic of science and techenology ; Branch court of Chongqing academic of science and techenology
- 年：2016
- 作者机构：重庆市畜牧科学院;重庆市畜牧科学院武陵分院;
- 论文关键词：黄芪多糖 ; 肉鹅 ; 禽流感疫苗 ; 免疫 ; 抗体水平
- 英文论文关键词：APS ; meat goose ; avian influenza vaccine ; immune ; antibody level
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S858.33
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：61k
- 原文格式：O
- 会议级别：全国
摘要
为了探讨黄芪多糖配合禽流感鹅的免疫效果的影响,本研究利用水提醇法对黄芪多糖进行有效提取,并对黄芪多糖的纯度进行测定,同时,将40只1日龄肉鹅随机分为两组,试验组肌肉注射禽流感疫苗和黄芪多糖溶液,对照组注射禽流感疫苗和生理盐水,分别在免疫后第10d、20d、30d后,检测其抗体水平,同时,对黄芪多糖的最适用量进行优化。结果发现,试验组的抗体水平均高于对照组,差异显著(P<0.05),对黄芪多糖的用量进行优化后,发现在其注射量为0.6mL/只时,试验组与对照组抗体水平差异极显著(P<0.01),试验表明,黄芪多糖对禽流感疫苗的免疫效果具有促进作用。
To explore the combined effect of APS and H5N1 avian influenza vaccine on the goose,APS was extracted with the water and alcohol.And the purity of APS was measured according to the standard curve of glucose.The 40 geese were random distributed into control group and treatment group.And the treatment group was immuned with APS and avian influenza vaccine,the control group was immuned with avian influenza vaccine and normal saline.And the antibody level was evaluated at 10,20,30 days after the first immunization,in addition,the best dosage of APS was researched according to the antibody level.It was found that the antibody level of the treatment group was significant different with the control group(P<0.05).And it was extremely significant different when the dosage of APS was 0.6mL each goose(P<0.01).The study proved that APS can promote the immune efficacy of avian influenza vaccine.
To explore the combined effect of APS and H5N1 avian influenza vaccine on the goose,APS was extracted with the water and alcohol.And the purity of APS was measured according to the standard curve of glucose.The 40 geese were random distributed into control group and treatment group.And the treatment group was immuned with APS and avian influenza vaccine,the control group was immuned with avian influenza vaccine and normal saline.And the antibody level was evaluated at 10,20,30 days after the first immunization,in addition,the best dosage of APS was researched according to the antibody level.It was found that the antibody level of the treatment group was significant different with the control group(P<0.05).And it was extremely significant different when the dosage of APS was 0.6mL each goose(P<0.01).The study proved that APS can promote the immune efficacy of avian influenza vaccine.
引文
[1]Nuowei Zhang,Jiefeng Li,Yanxin Hu,et al.Effects of astragalus polysaccharide on the immune response to foot-and-mouth disease vaccine in mice[J].Carbohydrate Polymers:Scientific and Technological Aspects of Industrially Important Polysaccharides,2010,82(3):680-686.
[2]杜继红,高亚军,王海良等.黄芪多糖粉对猪瘟疫苗免疫效果及生长性能的影响[J].中国畜牧杂志,2013,49(22):65-68.
[3]黄芪多糖分级组分的提取及其对鸡疫苗免疫效果的影响[J].西北农业学报,2009,18(6):39-42.
[2]杜继红,高亚军,王海良等.黄芪多糖粉对猪瘟疫苗免疫效果及生长性能的影响[J].中国畜牧杂志,2013,49(22):65-68.
[3]黄芪多糖分级组分的提取及其对鸡疫苗免疫效果的影响[J].西北农业学报,2009,18(6):39-42.