Kctd10与TBX蛋白相互作用调控房室间隔形成的研究
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- 英文论文题名：The interaction relationship between Kctd10 and TBX proteins during the AVC formation in zebrafish
- 论文作者：朱哲 ; 祖尧 ; 孟祥丽 ; 李伟明
- 英文论文作者：ZHU Zhe ; ZU Yao ; MENG Xiang-Li ; LI Wei-Ming ; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources ; Ministry of Education ; College of Fisheries and Life Science ; Shanghai Ocean University ; Department of Fisheries and Wildlife ; Michigan State University
- 年：2016
- 作者机构：上海海洋大学水产种质资源发掘与利用教育部重点实验室;密歇根州立大学渔业与野生生物系;
- 论文关键词：斑马鱼 ; 心脏环化 ; AVC ; kctd10 ; TBX家族
- 英文论文关键词：Zebrafish ; Heart looping ; AVC ; kctd10 ; TBX gene family
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：Q344
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：538k
- 原文格式：D
- 会议级别：全国
摘要
心脏是脊椎动物中最早形成并行使功能的器官之一。心脏发育过程中,心管经过复杂的环化过程,形成心房和心室间隔即AVC(atrioventricular canal),进而形成心脏的腔室结构,发育成有功能的心脏。已有的研究表明,斑马鱼中kctd10通过抑制tbx5a影响AVC的形成,小鼠中Tbx20和Tbx2b对AVC的形成也存在作用。为了探究斑马鱼AVC形成过程中kctd10与tbx5a、tbx20和tbx2b相关作用的关系,我们利用原位杂交技术检测了突变体kctd10中tbx2b在心脏的表达谱,通过免疫共沉淀技术检测了kctd10与tbx20蛋白的相互作用。结果显示,突变体kctd10中tbx2b基因在心脏的表达谱发生变化,kctd10与tbx20蛋白存在相互作用关系。利用CRISPR/Cas9基因编辑技术分别构建了tbx5a、tbx20和tbx2b的斑马鱼突变体,进一步研究AVC形成过程中,kctd10与TBX家族3个蛋白的相互作用关系。
Heart is one of the first formedfunctional organsinvertebrate. During its development,the heart tube undergoes looping to form atrioventricular canal(AVC) and finally develops into multi-chambered functional organ. Previous studies showed that kctd10 suppresses tbx5a to control AVC in zebrafish and both Tbx2 b and Tbx20 havesignificant effects on formation of AVC in mouse. The results of the in situ hybridization of tbx2 b showed that tbx2 b expression pattern had changed in the kctd10 mutant compared with the wild type zebrafish.The co-immunoprecipitation results indicated that the tbx20 has a interaction relationship with kctd10.Therefore,we constructed the mutants of the three TBX genes of tbx5 a, tbx20 and tbx2 brespectively using CRISPR/Cas9 gene editing technology to further study the relationship between kctd10 and the tree TBX genes during the process of AVC formation.
Heart is one of the first formedfunctional organsinvertebrate. During its development,the heart tube undergoes looping to form atrioventricular canal(AVC) and finally develops into multi-chambered functional organ. Previous studies showed that kctd10 suppresses tbx5a to control AVC in zebrafish and both Tbx2 b and Tbx20 havesignificant effects on formation of AVC in mouse. The results of the in situ hybridization of tbx2 b showed that tbx2 b expression pattern had changed in the kctd10 mutant compared with the wild type zebrafish.The co-immunoprecipitation results indicated that the tbx20 has a interaction relationship with kctd10.Therefore,we constructed the mutants of the three TBX genes of tbx5 a, tbx20 and tbx2 brespectively using CRISPR/Cas9 gene editing technology to further study the relationship between kctd10 and the tree TBX genes during the process of AVC formation.
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