rGO-SnO_2复合材料:一种双功能亲和探针对于内源性多肽和磷酸化多肽的高效富集
详细信息 查看官网全文
- 英文论文题名：Facile synthesis of rGO-SnO_2 composite:an appealing multifunctional affinity probe for sequential selective enrichment of endogenous peptides and phosphopeptides
- 论文作者：马雯 ; 张丰 ; 李丽萍 ; 白玉 ; 刘虎威
- 英文论文作者：Wen Ma ; Zhang Feng ; Liping Li ; Yu Bai ; Huwei Liu ; Department of Chemistry ; Peking University ; Department of Chemistry ; Capital Normal University
- 年：2016
- 作者机构：北京大学化学与分子工程学院;首都师范大学化学系;
- 论文关键词：内源性多肽 ; 磷酸化多肽 ; 复合材料 ; 质谱
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
- 语种：中文
- 分类号：TB332;O652.6
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：212k
- 原文格式：D
- 会议级别：全国
摘要
低丰度内源性多肽和磷酸化多肽在生理病理学过程中扮演着重要角色~1,体内这两种多肽含量的变化与疾病的发生发展息息相关,因此,对两者的研究在临床检测和生物标志物的发现上有重要意义。然而,内源性多肽的检测受基质干扰很大。另一方面,磷酸化多肽由于丰度低,离子化困难,受其他非磷酸化多肽信号抑制严重而难以用质谱进行直接检测~2。因此,在质谱分析之前,对样品进行选择性富集尤为重要。本工作通过一步水热反应法合成氧化石墨烯-二氧化锡复合物,再通过对其热处理得到还原氧化石墨烯-二氧化锡复合材料rGO-SnO_2。结合rGO的疏水性和SnO_2的高亲和性,该材料可实现对低丰度多肽和磷酸化多肽的分布富集。rGO-SnO_2总共能从BSA酶解液中富集到34条多肽,对磷酸化多肽的检出限达到4×10~(-10)M,成功实现对复杂生物样本人血清中的内源性多肽和磷酸化多肽的分布富集。该结果展示了复合材料的双功能作用,给样品前处理领域的材料设计提供了新的思路。
Low-abundance endogenous peptides and phosphopeptides play crucial roles in physiological and pathological processes.Changes in these two peptides are closely related to various disease initiation and progression,thus related research is important for clinical diagnostic and biomarker discovery.However,the analysis of endogenous peptides suffer from complex matrix and the low stoichiometry,poor ionization,and signal suppression make it difficult to analyse phosphorylation by mass spectrometry(MS) directly.Powerful sample preparation prior MS analysis is in indispensable.Herein,we synthesized composite rGO-SnO_2 through one-pot solventhermal process,followed by thermal reduction.With the hydrophobicity of rGO and high affinity of SnO_2,rGO-SnO_2 was used for sequential enrichment of endogenous peptides and phosphopeptides.As a result,34 peptides were captured from BSA digest and the LOD of phosphopeptides from β-casein digest was 4×10~(-10) M,the material showed feasibility in human serum.This multifunctional affinity probe opens a new perspectives in material design in sample preparation.
Low-abundance endogenous peptides and phosphopeptides play crucial roles in physiological and pathological processes.Changes in these two peptides are closely related to various disease initiation and progression,thus related research is important for clinical diagnostic and biomarker discovery.However,the analysis of endogenous peptides suffer from complex matrix and the low stoichiometry,poor ionization,and signal suppression make it difficult to analyse phosphorylation by mass spectrometry(MS) directly.Powerful sample preparation prior MS analysis is in indispensable.Herein,we synthesized composite rGO-SnO_2 through one-pot solventhermal process,followed by thermal reduction.With the hydrophobicity of rGO and high affinity of SnO_2,rGO-SnO_2 was used for sequential enrichment of endogenous peptides and phosphopeptides.As a result,34 peptides were captured from BSA digest and the LOD of phosphopeptides from β-casein digest was 4×10~(-10) M,the material showed feasibility in human serum.This multifunctional affinity probe opens a new perspectives in material design in sample preparation.
引文
[1]M,Swami.Nat.Rev.Cancer.2010,10:597.
[2]G,Han.;M,Ye.;H,Zou.Analyst.2008,133:1128.
[2]G,Han.;M,Ye.;H,Zou.Analyst.2008,133:1128.