重金属离子神经毒性的细胞代谢组学研究
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- 英文论文题名：Neurotoxic effect of cadmium,copper,lead on PC-12 cells based on cell metabonomics
- 论文作者：宗丽 ; 刘舒 ; 刘志强 ; 宋凤瑞
- 英文论文作者：Li Zong ; Shu Liu ; Zhiqiang Liu ; Fengrui Song ; National Center of Mass Spectrometry in Changchun ; Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry ; Changchun Institute of Applied Chemistry ; Chinese Academy of Sciences ; University of Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院长春应用化学研究所长春质谱中心&吉林省中药化学与质谱重点实验室;中国科学院大学;
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
- 语种：中文
- 分类号：R114
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：167k
- 原文格式：D
- 会议级别：全国
摘要
<正>重金属离子铜、铅和镉是有毒的环境和工业污染物,它们能够透过血脑屏障进入人脑并在脑内蓄积,极性和慢性暴露会导致神经毒性,从而引起各种脑、神经系统等疾病[1]。目前,有关它们神经毒性的报道很多,但是关于它们毒性作用的分子机制研究较少。我们运用细胞代谢组学的研究方法,以LC-MS为分析测
The heavy metals Cd~(2+),Pb ~(2+)or Cd~(2+) are toxic environmental and industrial pollutants.Acute or chronic exposure can result in neurotoxicity.However,the neurotoxicity mechanisms of these metals are still not clear.To explore the underlying mechanism,the neuronal PC-12 cells were used and treated with CuSO_4,PbCl_2 and CdCl_2.And the intracellular and extracellular metabolites were profiled using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.These heavy metals could give rise to excessive oxidative stress to lead to cell death.The metabolic changes were involved in phenylalanine and tryptophan metabolism,pantothenate and CoA biosynthesis,Pyrimidine metabolism,protein biosynthesis and the others.These results provide insight in the mechanism of neurotoxic research of heavy metals.
The heavy metals Cd~(2+),Pb ~(2+)or Cd~(2+) are toxic environmental and industrial pollutants.Acute or chronic exposure can result in neurotoxicity.However,the neurotoxicity mechanisms of these metals are still not clear.To explore the underlying mechanism,the neuronal PC-12 cells were used and treated with CuSO_4,PbCl_2 and CdCl_2.And the intracellular and extracellular metabolites were profiled using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.These heavy metals could give rise to excessive oxidative stress to lead to cell death.The metabolic changes were involved in phenylalanine and tryptophan metabolism,pantothenate and CoA biosynthesis,Pyrimidine metabolism,protein biosynthesis and the others.These results provide insight in the mechanism of neurotoxic research of heavy metals.
引文
[1]Jiang,C.;Yuan,Y.;Hu,F.;Wang,Q.;Zhang,K.;Wang,Y.;Gu,J.;Liu,X.;Bian,J.;Liu,Z.Biol Trace Elem Res.2014,158:249.