pH调节法优化鸢乌贼分离蛋白制备的工艺研究

详细信息    查看官网全文

• 英文论文题名：Optimization of preparation process of protein isolates from Sthenoteuthis oualaniensis by pH-shift methodology
• 论文作者：董淑华 ; 黄卉 ; 李来好 ; 杨贤庆 ; 魏涯 ; 赵永强 ; 林婉玲
• 英文论文作者：DONG Shu-hua ; HUANG Hui ; LI Lai-hao ; YANG Xian-qing ; WEI Ya ; ZHAO Yong-qiang ; LIN Wan-ling ; Key Laboratory of Aquatic Product Processing ; Ministry of Agriculture ; National R&D Center for Aquatic Product Processing ; South China Sea Fisheries Research Institute ; Chinese Academy of Fishery Science ; College of Food Science and Technology ; Shanghai Ocean University
• 年：2016
• 作者机构：中国水产科学研究院南海水产研究所农业部水产品加工重点实验室国家水产品加工技术研发中心;上海海洋大学食品学院;
• 论文关键词：鸢乌贼 ; 提取 ; 分离蛋白 ; pH调节法
• 英文论文关键词：Sthenoteuthis oualaniensis ; extraction ; protein isolates ; pH-shift
• 会议召开时间：2016-11-02
• 会议录名称：2016年中国水产学会学术年会论文摘要集
• 语种：中文
• 分类号：TS201.21
• 学会代码：OGSB
• 会议名称：2016年中国水产学会学术年会
• 会议地点：中国四川成都
• 主办单位：中国水产学会、四川省水产学会
• 学会名称：中国水产学会
• 页数：1
• 文件大小：505k
• 原文格式：D
• 会议级别：全国

摘要

研究了pH调节法对鸢乌贼分离蛋白制备的优化工艺,优化条件如下:在提取pH值12.0、提取温度4℃、提取时间10min、料液比1:9条件下,制备得到的分离蛋白溶解得率最高,达到80%;在最佳酸、碱溶解pH值前提下沉淀蛋白,pH值5.5沉淀条件下回收蛋白的得率最高,且酸沉得到的分离蛋白沉淀得率高达60%,大于碱沉;pH对鸢乌贼分离蛋白各组分的影响不同,肌球蛋白重链(MHC)、副肌球蛋白(PM)在各pH值条件下降解速率较快,酸性条件对原肌球蛋白(TM)降解效果影响较大,而肌动蛋白(A)在酸、碱性环境下变化较不明显。
The paper studied optimization of preparation process of protein isolates from Sthenoteuthis oualaniensis by pH-shift methodology.The results of optimum preparation conditions were as follow:the water to raw material 1:9(g/mL),extraction pH value 12.0,extraction temperature 4℃ and extraction time 10 min.Result showed that dissolution rate of isolated protein was the highest and reached 80%.The yield of protein recovered was the highest when pH reached 5.5 with the optimum condition of acid or alkaline dissolution pH value and the precipitation rate of isolated protein precipitated by acid precipitation is as high as 60%, which is higher than that of alkaline precipitation. The effects of different pH values on the components of protein isolates from Sthenoteuthis oualaniensis were different. Myosin heavy chain(MHC) and paramyosin(PM) were affected by different pH values and degradated quickly, actin(A) was less affected by acid and alkaline conditions, Tropomyosin(TM) was affected by acidic conditions and the degradation effect was great.

引文