鹿科与牛科动物IGF-1基因序列比较及分子进化分析
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- 英文论文题名：Sequence Compariation and Molecular Evolution Analysis of IGF-1 Gene Between Cervidae and Bovidae Species
- 论文作者：宋兴超 ; 徐超 ; 王雷 ; 巴恒星 ; 王桂武 ; 杨福合
- 英文论文作者：SONG Xing-chao ; XU Chao ; WANG Lei ; Ba Heng-xing ; WANG Gui-wu ; YANG Fu-he ; State Key Laboratory of Special Economic Animal Molecular Biology ; Institute of Special Animal and Plant Science of CAAS
- 年：2016
- 作者机构：中国农业科学院特产研究所吉林省特种经济动物分子生物学重点实验室;
- 论文关键词：鹿科 ; 牛科 ; 胰岛素样生长因子-1基因 ; 序列比较 ; 密码子
- 英文论文关键词：Cervidae ; Bovidae ; IGF-1 gene ; Sequence compariation ; Codon
- 会议召开时间：2016-06-01
- 会议录名称：“十二五” 鹿科学研究进展
- 语种：中文
- 分类号：S852.2
- 学会代码：ZXMY
- 学会名称：中国畜牧业协会
- 页数：7
- 文件大小：874k
- 原文格式：O
摘要
以GenBank中已登录的梅花鹿(Cervus nippon)、马鹿(Cervs elaphus)、普通牛(Bos taurus)、水牛(Bubalus bubalis)、山羊(Capra hircus)和绵羊(Ovis aries)胰岛素样生长因子-1(IGF-1)基因mRNA序列为研究材料,通过DNAStar7.0等生物信息学软件对6个物种的IGF-1基因进行序列比较及分子进化分析。结果表明:(1)鹿科与牛科动物IGF-1基因编码区均为465 bp,并且碱基组成表现为G>C>A>T,且G+C含量高于A+T;(2)6个物种IGF-1基因密码子第1位富含A,密码子第2位4种碱基的分布相对均匀,密码子的第3位碱基C含量较高;(3)编码区碱基序列中共检测到20个变异位点,包括10个单一变异和10个简约变异,鹿科和牛科动物IGF-1基因核苷酸和氨基酸序列的相似性均较高;(4)NJ和UPGMA两种方法构建的分子进化树均把6个物种聚为2个大类:鹿科和牛科,其中牛科又分支出牛亚科和羊亚科两类。
Based on the IGF- 1 gene nucleotide and amino acid sequences of Cervus nppon,Cervus elaphus,Bos taurus,Bubalus bubalis,Capra hircus and Ovis aries registed in GenBank,sequence variation and molecular evolution of IGF- 1 gene were analyzed by means of bioinformatics software.The results showed that:(1) The length of Cervidae and Bovidae species IGF- 1 gene coding sequence is 465 bp,which encodes 154 amino acid.Base composition is G>C>A>T and the content of G + C is higher than that of A + T.(2) The first position of IGF- 1 gene codon in 6 species contains A rich,and the four base in the second position distributed relatively even,however,there is higher base C content in the third position.(3) A total of 20 polymorphic sites,including 10 singleton sites and 10 parsimony informative sites were detected among 6 species.In addition,there are higher similarity of nucleotide and amino acid sequence between Cervidae and Bovidae species.(4) The molecular evolutionary tree of 6 species was constructed by NJ and UPGMA method that together into two broad categories:Cervidae and Bovidae,in which Bovidae and Capinae were belong to the Bovidae.
Based on the IGF- 1 gene nucleotide and amino acid sequences of Cervus nppon,Cervus elaphus,Bos taurus,Bubalus bubalis,Capra hircus and Ovis aries registed in GenBank,sequence variation and molecular evolution of IGF- 1 gene were analyzed by means of bioinformatics software.The results showed that:(1) The length of Cervidae and Bovidae species IGF- 1 gene coding sequence is 465 bp,which encodes 154 amino acid.Base composition is G>C>A>T and the content of G + C is higher than that of A + T.(2) The first position of IGF- 1 gene codon in 6 species contains A rich,and the four base in the second position distributed relatively even,however,there is higher base C content in the third position.(3) A total of 20 polymorphic sites,including 10 singleton sites and 10 parsimony informative sites were detected among 6 species.In addition,there are higher similarity of nucleotide and amino acid sequence between Cervidae and Bovidae species.(4) The molecular evolutionary tree of 6 species was constructed by NJ and UPGMA method that together into two broad categories:Cervidae and Bovidae,in which Bovidae and Capinae were belong to the Bovidae.
引文
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[2]Duan C M,Ren H X,Gao S.Insulin-like growth factors(IGFs),IGF receptors,and IGFbinding proteins;Roles in skeletal muscle growth and differentiation[J].General and Comparative Endocrinology,2010,167:344-351.
[3]Niu P X,Kim S W,Choi B H,et al.Porcine insulin-like growth factor 1(IGFI)gene polymorphisms are associated with body size variation[J].Genes Genom,2013,35:523-528.
[4]Maskur,Arman C,Sumantri C,et al.A novel single nucleotide polymorphism in exon 4 of insulin-like growth factor-1 associated with production traits in bali cattle[J].Media Peternakan,2012,8:96-101.
[5]Bahrami A,Behzadi S,Miraei-Ashtiani S R,Roh S G,et al.Genetic polymorphisms and protein structures in growth hormone,growth hormone receptor,ghrelin,insulin-like growth factor 1and leptin in mehraban sheep[J].Gene,2013,527:397-404.
[6]Paswan C,Bhattacharya T K,Nagaraja C S,et al.Nucleotide variability in partial promoter of IGF-1 gene and its association with body weight in fast growing chicken[J].Animal Research,2013,3(1):31-36.
[7]杜智恒,王宇祥,白秀娟.梅花鹿胰岛素样生长因子I(IGF1)基因的克隆及序列分析[J].黑龙江畜牧兽医,2007,5:98-99.
[8]胡薇,孟星宇,田玉华,等.梅花鹿IGF1全长eDNA克隆及在鹿茸组织的表达[J].东北林业大学学报,2011,39(11):71-75.
[9]郝林琳,刘松财,赵志辉,等.马鹿茸IGF-1基因端克隆及生物信息学分析[J].畜牧与兽医,2007,39(12):47-49.
[10]李赵志,严昌国,张立春,等.吉林梅花鹿IGF-1基因启动子区单核苷酸多态性分析[J].安徽农业科学,2010,38(13):6 729-6 730.
[11]王宁,陈润生.基于内含子和外显子的系统发育分析的比较[J].科学通报,1999,44(19):2 095-2 102.
[12]邹思湘.动物生物化学[M].北京,中国农业出版社,第四版,2005:287-306.
[13]李鹏飞,周永东,徐汉祥.大黄鱼、鮸鱼及美国红鱼线粒体DNA的Cyt b基因序列比较[J].南方水产,2008,4(3):43-47.
[14]赵世臻.中国养鹿大成[M].北京:中国农业出版社,2001:257-265.
[15]杨秋丽,哈福,李大林,等.牛科物种FSHb基因外显子3编码区遗传多态性及其生物信息学分析[J].华北农学报,2013,28(2):52-59.
[16]Wada K,Nishibori M,Yokohama M.The complete nucleotide of mitochondrial genome in the Japanese Sika deer(Cervus nippon),and a phylogenetic analysis between Cervidae and Bovidae[J].Small Ruminant Research,2007,69:46-54.
[17]Klungland H,ROED K H,NESBO C,et al.The melanocyte-stimulating hormone receptor(MC1-R)gene as a tool in evolutionary studies of Artiodactyles[J].Hereditas,1999,131;39-46.
[18]侯佩兴,孙立彬,赵洪波.偶蹄目牛科和鹿科SRY基因编码区进化研究[J].上海交通大学学报(农业科学版),2009,27(4):350-352.